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The map label for this gene is rlmH

Identifier: 227081125

GI number: 227081125

Start: 973559

End: 974029

Strand: Reverse

Name: rlmH

Synonym: VCM66_0907

Alternate gene names: 227081125

Gene position: 974029-973559 (Counterclockwise)

Preceding gene: 227081126

Following gene: 227081124

Centisome position: 33.67

GC content: 51.38

Gene sequence:

>471_bases
GTGAAGATTCAACTGATCGCCGTCGGTACTAAGATGCCTAAATGGGTCGAAGAAGGCTTCCAAGAGTATCGTCGTCGCTT
CCCTCATGATATGCCTTTGGAATTGGTAGAAATTACCGCCGGTAAACGCGGTAAAAATGCTGATATTGCGCGTATCTTAC
AAAAAGAGGGCGAAGCGATGCTGGCCGCAGTGCCCAAAGGCAATCGAATCGTCACCTTAGATATTCCCGGCAAACGTTGG
GATACGGAAGAGCTCGCAGTACAGTTGGAAAGCTGGAAACTGGATGGGCGTGACGTCTCGATCCTGATTGGTGGACCAGA
AGGCCTTGCGCCCGCCTGTAAAGCCGCGGCAGACCAAAGTTGGTCTCTTTCTCCACTGACCTTGCCTCATCCACTAGTGC
GCGTGGTGATGGCAGAAAGTTTATACCGTGCGTGGAGCATTACTACTAACCACCCTTACCACCGAGAATAG

Upstream 100 bases:

>100_bases
AAGGTGAATGGGTCGTTCTGGATATGGGTAGCGTCATGTTGCATGTGATGCAAGAAGCGCCACGCGAACTCTATCAATTG
GAAAAACTCTGGGGTTAAGT

Downstream 100 bases:

>100_bases
AGTGACATGTTACGCCAGCATACCCCTATCCGCGACCATCAAGCCGAAACGCATCTGTTTCGTAATCGTGCCATCGTCTC
TTTTGTCGGGATCTTGATCT

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKIQLIAVGTKMPKWVEEGFQEYRRRFPHDMPLELVEITAGKRGKNADIARILQKEGEAMLAAVPKGNRIVTLDIPGKRW
DTEELAVQLESWKLDGRDVSILIGGPEGLAPACKAAADQSWSLSPLTLPHPLVRVVMAESLYRAWSITTNHPYHRE

Sequences:

>Translated_156_residues
MKIQLIAVGTKMPKWVEEGFQEYRRRFPHDMPLELVEITAGKRGKNADIARILQKEGEAMLAAVPKGNRIVTLDIPGKRW
DTEELAVQLESWKLDGRDVSILIGGPEGLAPACKAAADQSWSLSPLTLPHPLVRVVMAESLYRAWSITTNHPYHRE
>Mature_156_residues
MKIQLIAVGTKMPKWVEEGFQEYRRRFPHDMPLELVEITAGKRGKNADIARILQKEGEAMLAAVPKGNRIVTLDIPGKRW
DTEELAVQLESWKLDGRDVSILIGGPEGLAPACKAAADQSWSLSPLTLPHPLVRVVMAESLYRAWSITTNHPYHRE

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=156, Percent_Identity=81.4102564102564, Blast_Score=261, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_VIBC3 (A5F2Y7)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F2Y7
- SMR:   A5F2Y7
- STRING:   A5F2Y7
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0474
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- BioCyc:   VCHO345073:VC0395_A0474-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17521; Mature: 17521

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQLIAVGTKMPKWVEEGFQEYRRRFPHDMPLELVEITAGKRGKNADIARILQKEGEAM
CEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEE
LAAVPKGNRIVTLDIPGKRWDTEELAVQLESWKLDGRDVSILIGGPEGLAPACKAAADQS
EEECCCCCEEEEEECCCCCCCHHHHHEEHHHEECCCCEEEEEEECCCCCCHHHHHHCCCC
WSLSPLTLPHPLVRVVMAESLYRAWSITTNHPYHRE
CCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCC
>Mature Secondary Structure
MKIQLIAVGTKMPKWVEEGFQEYRRRFPHDMPLELVEITAGKRGKNADIARILQKEGEAM
CEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEE
LAAVPKGNRIVTLDIPGKRWDTEELAVQLESWKLDGRDVSILIGGPEGLAPACKAAADQS
EEECCCCCEEEEEECCCCCCCHHHHHEEHHHEECCCCEEEEEEECCCCCCHHHHHHCCCC
WSLSPLTLPHPLVRVVMAESLYRAWSITTNHPYHRE
CCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA