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The map label for this gene is smpB

Identifier: 227081024

GI number: 227081024

Start: 872178

End: 872663

Strand: Reverse

Name: smpB

Synonym: VCM66_0805

Alternate gene names: 227081024

Gene position: 872663-872178 (Counterclockwise)

Preceding gene: 227081027

Following gene: 227081023

Centisome position: 30.17

GC content: 48.35

Gene sequence:

>486_bases
ATGACAAAAAAGAAAACCAATACCAAAGCGGGTAGCAACACTATCGCGCTGAACAAAAAAGCTCGCCACGAATACTTCAT
CGAAGATGAATTTGAAGCCGGCATGGAGCTACAAGGCTGGGAAGTCAAATCACTTCGCCAAGGTAAAGCCAACATTGCAG
AAAGCTACGTCTACATCAAAGATGGCGAAGCGTTCATTTCCGGTATGACCATCATTCCGTTGCAGCAAGCCTCCACTCAC
GTGGTAGCCAACCCTACCCGTATCCGCAAGCTGCTACTTTCACGCCGCGAGCTGGACAACCTGTTTGGCCGCATCAACCG
TGAAGGGATGACACTAACCGCATTGTCACTCTACTGGTCTCGTTCGTGGGTCAAAATCAAAATTGGCGTTGCGAAAGGTA
AGAAACTGCACGACAAACGTGAAGATCTGAAAGAAAAAGAGTGGCAACGCCAAAAAGATCGAGTGATGAAGAGCGCGTTG
CGCTAA

Upstream 100 bases:

>100_bases
ACAACACAATACAGCGCTTGAGTCATCGCGCAGATAACCGTATGCTACGGCAAACAAAAGCGTTTCCTAACCGCTGACCA
ATCCGTATAATGCGCCCACT

Downstream 100 bases:

>100_bases
TAGTAACCACTTGAAAAATGGGAGCTAGACAGAAGCACTTATTCTGGTACTATGCACCCGTTACCTCTGGGGCTGATTCA
GGATTCGACGGGAATTTTGC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MTKKKTNTKAGSNTIALNKKARHEYFIEDEFEAGMELQGWEVKSLRQGKANIAESYVYIKDGEAFISGMTIIPLQQASTH
VVANPTRIRKLLLSRRELDNLFGRINREGMTLTALSLYWSRSWVKIKIGVAKGKKLHDKREDLKEKEWQRQKDRVMKSAL
R

Sequences:

>Translated_161_residues
MTKKKTNTKAGSNTIALNKKARHEYFIEDEFEAGMELQGWEVKSLRQGKANIAESYVYIKDGEAFISGMTIIPLQQASTH
VVANPTRIRKLLLSRRELDNLFGRINREGMTLTALSLYWSRSWVKIKIGVAKGKKLHDKREDLKEKEWQRQKDRVMKSAL
R
>Mature_160_residues
TKKKTNTKAGSNTIALNKKARHEYFIEDEFEAGMELQGWEVKSLRQGKANIAESYVYIKDGEAFISGMTIIPLQQASTHV
VANPTRIRKLLLSRRELDNLFGRINREGMTLTALSLYWSRSWVKIKIGVAKGKKLHDKREDLKEKEWQRQKDRVMKSALR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=161, Percent_Identity=69.5652173913043, Blast_Score=237, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_VIBC3 (A5F375)

Other databases:

- EMBL:   U39068
- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F375
- SMR:   A5F375
- STRING:   A5F375
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0374
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   VCHO345073:VC0395_A0374-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18637; Mature: 18506

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKKTNTKAGSNTIALNKKARHEYFIEDEFEAGMELQGWEVKSLRQGKANIAESYVYIK
CCCCCCCCCCCCCEEEECCCCCCCCCCCCHHCCCCCCCCCCHHHHHCCCCCHHHCEEEEE
DGEAFISGMTIIPLQQASTHVVANPTRIRKLLLSRRELDNLFGRINREGMTLTALSLYWS
CCHHHHCCEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHC
RSWVKIKIGVAKGKKLHDKREDLKEKEWQRQKDRVMKSALR
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKKKTNTKAGSNTIALNKKARHEYFIEDEFEAGMELQGWEVKSLRQGKANIAESYVYIK
CCCCCCCCCCCCEEEECCCCCCCCCCCCHHCCCCCCCCCCHHHHHCCCCCHHHCEEEEE
DGEAFISGMTIIPLQQASTHVVANPTRIRKLLLSRRELDNLFGRINREGMTLTALSLYWS
CCHHHHCCEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHC
RSWVKIKIGVAKGKKLHDKREDLKEKEWQRQKDRVMKSALR
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA