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The map label for this gene is intA [H]

Identifier: 227081023

GI number: 227081023

Start: 870308

End: 871576

Strand: Reverse

Name: intA [H]

Synonym: VCM66_0804

Alternate gene names: 227081023

Gene position: 871576-870308 (Counterclockwise)

Preceding gene: 227081024

Following gene: 227081021

Centisome position: 30.13

GC content: 46.34

Gene sequence:

>1269_bases
ATGGCAAAAAACACAGTAAAGCTGACAGATAAAGAGATCAAAGCCTCCAAACCTAGAGAGAAAGAATATAATCTTTTCGA
TGGTGATGGCTTGCGACTTAGAATCAAACCTAACGGTTCAAAGCAGTGGATCTTCAACTACTATCGCCCCACAAACGGTA
AACGAGCTAACTTAAGCTTTGGAATGTACCCAGACATCTCCCTTTTACAAGCAAGAAAAAGCTTACTTTCTGCCAAAGAG
CTGATCGCACAAGGCATCGATCCACAAGACGATCGAAAACGCCAACAACAAGTACATAAAGAGATCCATGAGCACACCTT
TGAGAACGTCACAAAGGATTGGTTTGCCATCAAACAGAATGATGTGACACCGGATTACGCCCTTGATATCTGGCGTTCAT
TAGAGCTACATATCTTCCCGCACATTTCCGACAAACCGGTAAAAGCGATCACCGCTCCGGAGATCATAGAACTACTCAAA
CCGATTGAAGCCAAAGGTAGCCTAGAAACCGTCAAACGTTTAACACAGCGACTCAATGAAGTGATGAACTTCGCCACTAA
CTGCGGTCTTATCCAGGCCAATCCATTAACTGGCATCAAAGCCGCCTTCAAGAAACCAAAAAAAGAAAACATGGCAGCAC
TGACACCAGCAGAACTGCCCGAACTCATGAGCGCAATCGCTAATGCCAGTATCAAACGCACTACCCGTTGTTTACTTGAG
TGGCAACTACACACCATGACGCGCCCAGCGGAAGCATCTGGTGCACGTTGGGATGAAATTAATTGGGAAGAAAAGGTTTG
GACCATTCCTGCTGAAAGAATGAAAAAAAGAAGAGAACACCGCATCCCGCTCACAGAGCAGATGCTGGCGCTATTGGAAG
TAATGAAACCGATAAGTGGCCATCGTGATTTTATTTTTCCGTCGGATAGAGCCCCGAAAAAGCCCTGCAACAGCCAAACG
GCAAATATGGCATTGAAACGTATGGGGTTTGCTGGAAGGCTGGTTAGTCACGGTTTGCGCTCTCTAGCGAGTACCACACT
CAACGAACAAGGCTTTGATCCAGACTTGGTTGAGTCGGCATTGGCTCACGTGGACGATAACCAAGTACGCAGTGCCTATA
ACCGCACTGACTACTTAGAACGCAGAAAACCAATGATGTGCTGGTGGAGCGAGCATATAGAAGAGGCAGCCAGAGGTAGC
CTATCTGTGACTGGTACAAAGCAATTGAAAAATAAACTTCCACCGCCCCCCACATGGAAGCAGATGTGA

Upstream 100 bases:

>100_bases
TTAGCATTCATAGCTATTCCACAAGATTCCATAGCAGCTTTGATTTTTAGGTACACTACAAGGTACACTGATTTTATCGT
GTTAATCTGGTGTACCCATC

Downstream 100 bases:

>100_bases
TCCTAACGGCATTCTTTTTGTTCTAAGAACTGGTTGTCAGTGGAATACGCTTAACGCGGCGAGTATTTTTACCTCTAGCT
CTGCACATCGTCGCTTTCAA

Product: integrase, phage family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MAKNTVKLTDKEIKASKPREKEYNLFDGDGLRLRIKPNGSKQWIFNYYRPTNGKRANLSFGMYPDISLLQARKSLLSAKE
LIAQGIDPQDDRKRQQQVHKEIHEHTFENVTKDWFAIKQNDVTPDYALDIWRSLELHIFPHISDKPVKAITAPEIIELLK
PIEAKGSLETVKRLTQRLNEVMNFATNCGLIQANPLTGIKAAFKKPKKENMAALTPAELPELMSAIANASIKRTTRCLLE
WQLHTMTRPAEASGARWDEINWEEKVWTIPAERMKKRREHRIPLTEQMLALLEVMKPISGHRDFIFPSDRAPKKPCNSQT
ANMALKRMGFAGRLVSHGLRSLASTTLNEQGFDPDLVESALAHVDDNQVRSAYNRTDYLERRKPMMCWWSEHIEEAARGS
LSVTGTKQLKNKLPPPPTWKQM

Sequences:

>Translated_422_residues
MAKNTVKLTDKEIKASKPREKEYNLFDGDGLRLRIKPNGSKQWIFNYYRPTNGKRANLSFGMYPDISLLQARKSLLSAKE
LIAQGIDPQDDRKRQQQVHKEIHEHTFENVTKDWFAIKQNDVTPDYALDIWRSLELHIFPHISDKPVKAITAPEIIELLK
PIEAKGSLETVKRLTQRLNEVMNFATNCGLIQANPLTGIKAAFKKPKKENMAALTPAELPELMSAIANASIKRTTRCLLE
WQLHTMTRPAEASGARWDEINWEEKVWTIPAERMKKRREHRIPLTEQMLALLEVMKPISGHRDFIFPSDRAPKKPCNSQT
ANMALKRMGFAGRLVSHGLRSLASTTLNEQGFDPDLVESALAHVDDNQVRSAYNRTDYLERRKPMMCWWSEHIEEAARGS
LSVTGTKQLKNKLPPPPTWKQM
>Mature_421_residues
AKNTVKLTDKEIKASKPREKEYNLFDGDGLRLRIKPNGSKQWIFNYYRPTNGKRANLSFGMYPDISLLQARKSLLSAKEL
IAQGIDPQDDRKRQQQVHKEIHEHTFENVTKDWFAIKQNDVTPDYALDIWRSLELHIFPHISDKPVKAITAPEIIELLKP
IEAKGSLETVKRLTQRLNEVMNFATNCGLIQANPLTGIKAAFKKPKKENMAALTPAELPELMSAIANASIKRTTRCLLEW
QLHTMTRPAEASGARWDEINWEEKVWTIPAERMKKRREHRIPLTEQMLALLEVMKPISGHRDFIFPSDRAPKKPCNSQTA
NMALKRMGFAGRLVSHGLRSLASTTLNEQGFDPDLVESALAHVDDNQVRSAYNRTDYLERRKPMMCWWSEHIEEAARGSL
SVTGTKQLKNKLPPPPTWKQM

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=410, Percent_Identity=53.4146341463415, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI145693166, Length=399, Percent_Identity=33.0827067669173, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788690, Length=392, Percent_Identity=29.8469387755102, Blast_Score=202, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 48363; Mature: 48232

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNTVKLTDKEIKASKPREKEYNLFDGDGLRLRIKPNGSKQWIFNYYRPTNGKRANLSF
CCCCCEEECCHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEECCCC
GMYPDISLLQARKSLLSAKELIAQGIDPQDDRKRQQQVHKEIHEHTFENVTKDWFAIKQN
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
DVTPDYALDIWRSLELHIFPHISDKPVKAITAPEIIELLKPIEAKGSLETVKRLTQRLNE
CCCCHHHHHHHHCCCEEEECCCCCCCCHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
VMNFATNCGLIQANPLTGIKAAFKKPKKENMAALTPAELPELMSAIANASIKRTTRCLLE
HHHHHHCCCEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
WQLHTMTRPAEASGARWDEINWEEKVWTIPAERMKKRREHRIPLTEQMLALLEVMKPISG
HHHHHCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
HRDFIFPSDRAPKKPCNSQTANMALKRMGFAGRLVSHGLRSLASTTLNEQGFDPDLVESA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LAHVDDNQVRSAYNRTDYLERRKPMMCWWSEHIEEAARGSLSVTGTKQLKNKLPPPPTWK
HHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCHHHHHHCCCCCCCCC
QM
CC
>Mature Secondary Structure 
AKNTVKLTDKEIKASKPREKEYNLFDGDGLRLRIKPNGSKQWIFNYYRPTNGKRANLSF
CCCCEEECCHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEECCCC
GMYPDISLLQARKSLLSAKELIAQGIDPQDDRKRQQQVHKEIHEHTFENVTKDWFAIKQN
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
DVTPDYALDIWRSLELHIFPHISDKPVKAITAPEIIELLKPIEAKGSLETVKRLTQRLNE
CCCCHHHHHHHHCCCEEEECCCCCCCCHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
VMNFATNCGLIQANPLTGIKAAFKKPKKENMAALTPAELPELMSAIANASIKRTTRCLLE
HHHHHHCCCEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
WQLHTMTRPAEASGARWDEINWEEKVWTIPAERMKKRREHRIPLTEQMLALLEVMKPISG
HHHHHCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
HRDFIFPSDRAPKKPCNSQTANMALKRMGFAGRLVSHGLRSLASTTLNEQGFDPDLVESA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LAHVDDNQVRSAYNRTDYLERRKPMMCWWSEHIEEAARGSLSVTGTKQLKNKLPPPPTWK
HHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCHHHHHHCCCCCCCCC
QM
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]