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The map label for this gene is citE [H]

Identifier: 227080977

GI number: 227080977

Start: 812017

End: 812907

Strand: Direct

Name: citE [H]

Synonym: VCM66_0756

Alternate gene names: 227080977

Gene position: 812017-812907 (Clockwise)

Preceding gene: 227080976

Following gene: 227080978

Centisome position: 28.07

GC content: 54.88

Gene sequence:

>891_bases
GTGGAGCCAGCTATCATGACCAAACTTCGTCGCAGTATGTTATTTGTGCCGGGCGCGAATGCCGCCATGCTCAGCAACAC
CTTTATCTATCAACCGGATTCGATCATGTTCGATCTGGAAGACTCGGTCGCGTTGCGTGAAAAAGACACCGCGCGGATGT
TAGTGTTCCATGCGCTGCAGCACCCTATGTACCAAGAGATGGAAACCGTGGTACGGGTCAATCCGCTCGATTCTGAATTC
GGCATCAAAGACCTTAATGCCGTGGTGCGCGGCGGAGCCAAAGTGGTGCGTCTGCCGAAAACCGATAACGCCAACGATGT
GATTGAGATGGAAAAAGTCATCGAGCAGATCGAGCGTGCATGCGGACGTGAAGTGGGTAGCACGCGTATGTTGGCGGCGA
TTGAAAGCGCGCAAGGCATCAACAACGCGGTTGAGATTGCCTTTAGCTCACGCCGCCTGATTGGGATTGCGCTCGGCGCG
GAAGACTATGTGCGCGATCTGCGCACGCAGCGTTCAGCGGAAGGGATTGAGCTGCTGTTTGCACGCTGCTCCATTCTGCA
AGCGGCGCGTGCTGCCGGGATCATGGCGTTTGATACCGTCTATTCCGATGCCAATAACGAAGAGGGATTTTTGCGTGAAG
CGGAGCACATCAAGCAGCTTGGCTTTGATGGCAAGTCACTGATTAACCCGCGCCAGATTGACCTGCTGCACAACGTGTTT
GCTCCAACCCAAAAAGAGGTCGATCACGCCATCGCAGTCATCGAAGCCGCCGAAGAAGCCGCAGCGAAAGGGCTAGGCGT
GGTTTCCCTTAACGGCAAAATGGTCGATGGCCCCATCATCGAACGCGCACGTTGGACACTGCAACGTGCTGAATCCGGCA
TCAAACACTAA

Upstream 100 bases:

>100_bases
ATGCGCTGGTCACCATCGAAGACAAAGGTGCGCTGGATTGTGTGATCCGCGCGCGCGTACAGGCGGCGGTGATGCGCGCT
TGTGATGTTCAGAACATTGA

Downstream 100 bases:

>100_bases
AGGTCATCTCATGAGTATCAATACATTAAATAACCCTCTGTCTGTGCTGGAAGTGGAACGCTACGCGTTGACGCCTTACA
CCCAAGCGCGCGCGATCACG

Product: citrate lyase, beta subunit

Products: NA

Alternate protein names: Citrase beta chain; Citrate (pro-3S)-lyase subunit beta; Citryl-CoA lyase subunit [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MEPAIMTKLRRSMLFVPGANAAMLSNTFIYQPDSIMFDLEDSVALREKDTARMLVFHALQHPMYQEMETVVRVNPLDSEF
GIKDLNAVVRGGAKVVRLPKTDNANDVIEMEKVIEQIERACGREVGSTRMLAAIESAQGINNAVEIAFSSRRLIGIALGA
EDYVRDLRTQRSAEGIELLFARCSILQAARAAGIMAFDTVYSDANNEEGFLREAEHIKQLGFDGKSLINPRQIDLLHNVF
APTQKEVDHAIAVIEAAEEAAAKGLGVVSLNGKMVDGPIIERARWTLQRAESGIKH

Sequences:

>Translated_296_residues
MEPAIMTKLRRSMLFVPGANAAMLSNTFIYQPDSIMFDLEDSVALREKDTARMLVFHALQHPMYQEMETVVRVNPLDSEF
GIKDLNAVVRGGAKVVRLPKTDNANDVIEMEKVIEQIERACGREVGSTRMLAAIESAQGINNAVEIAFSSRRLIGIALGA
EDYVRDLRTQRSAEGIELLFARCSILQAARAAGIMAFDTVYSDANNEEGFLREAEHIKQLGFDGKSLINPRQIDLLHNVF
APTQKEVDHAIAVIEAAEEAAAKGLGVVSLNGKMVDGPIIERARWTLQRAESGIKH
>Mature_296_residues
MEPAIMTKLRRSMLFVPGANAAMLSNTFIYQPDSIMFDLEDSVALREKDTARMLVFHALQHPMYQEMETVVRVNPLDSEF
GIKDLNAVVRGGAKVVRLPKTDNANDVIEMEKVIEQIERACGREVGSTRMLAAIESAQGINNAVEIAFSSRRLIGIALGA
EDYVRDLRTQRSAEGIELLFARCSILQAARAAGIMAFDTVYSDANNEEGFLREAEHIKQLGFDGKSLINPRQIDLLHNVF
APTQKEVDHAIAVIEAAEEAAAKGLGVVSLNGKMVDGPIIERARWTLQRAESGIKH

Specific function: Represents a citryl-ACP lyase [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=295, Percent_Identity=27.1186440677966, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI87081764, Length=290, Percent_Identity=77.5862068965517, Blast_Score=444, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17557458, Length=299, Percent_Identity=29.4314381270903, Blast_Score=123, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR006475
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.6; =4.1.3.34 [H]

Molecular weight: Translated: 32663; Mature: 32663

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPAIMTKLRRSMLFVPGANAAMLSNTFIYQPDSIMFDLEDSVALREKDTARMLVFHALQ
CCCHHHHHHHHHEEECCCCCHHHHCCEEEECCCCEEEECCCCHHHHCCHHHHHHHHHHHH
HPMYQEMETVVRVNPLDSEFGIKDLNAVVRGGAKVVRLPKTDNANDVIEMEKVIEQIERA
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
CGREVGSTRMLAAIESAQGINNAVEIAFSSRRLIGIALGAEDYVRDLRTQRSAEGIELLF
HCCCCCCHHHHHHHHHHCCCCHHEEEEECCCEEEEEEECCHHHHHHHHHHHCHHHHHHHH
ARCSILQAARAAGIMAFDTVYSDANNEEGFLREAEHIKQLGFDGKSLINPRQIDLLHNVF
HHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHH
APTQKEVDHAIAVIEAAEEAAAKGLGVVSLNGKMVDGPIIERARWTLQRAESGIKH
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEPAIMTKLRRSMLFVPGANAAMLSNTFIYQPDSIMFDLEDSVALREKDTARMLVFHALQ
CCCHHHHHHHHHEEECCCCCHHHHCCEEEECCCCEEEECCCCHHHHCCHHHHHHHHHHHH
HPMYQEMETVVRVNPLDSEFGIKDLNAVVRGGAKVVRLPKTDNANDVIEMEKVIEQIERA
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
CGREVGSTRMLAAIESAQGINNAVEIAFSSRRLIGIALGAEDYVRDLRTQRSAEGIELLF
HCCCCCCHHHHHHHHHHCCCCHHEEEEECCCEEEEEEECCHHHHHHHHHHHCHHHHHHHH
ARCSILQAARAAGIMAFDTVYSDANNEEGFLREAEHIKQLGFDGKSLINPRQIDLLHNVF
HHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHH
APTQKEVDHAIAVIEAAEEAAAKGLGVVSLNGKMVDGPIIERARWTLQRAESGIKH
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]