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The map label for this gene is citF [H]

Identifier: 227080978

GI number: 227080978

Start: 812843

End: 814447

Strand: Direct

Name: citF [H]

Synonym: VCM66_0757

Alternate gene names: 227080978

Gene position: 812843-814447 (Clockwise)

Preceding gene: 227080977

Following gene: 227080979

Centisome position: 28.1

GC content: 52.59

Gene sequence:

>1605_bases
ATGGCCCCATCATCGAACGCGCACGTTGGACACTGCAACGTGCTGAATCCGGCATCAAACACTAAAGGTCATCTCATGAG
TATCAATACATTAAATAACCCTCTGTCTGTGCTGGAAGTGGAACGCTACGCGTTGACGCCTTACACCCAAGCGCGCGCGA
TCACGCCGCATCTGCACGATGAGCAAGCGAAAAAAAGCCGCAAAGTGAAAAGCTCGATCCGCGAAGCGATTGTCGATCTT
GGCTTAAAAGATGGCATGACCATCTCTTTCCACCATGCGTTTCGTGGCGGCGATAAAACCGTCAATCTGGTGATGGAGGT
GATTGCTGAATTGGGAATTAAAAATCTCACGCTCGCCTCAAGCTCGCTGACCTCTTGTCATTCGCCTTTGATTGAACACA
TCAAAAACGGCGTAGTGAGCAAGATTTACACCTCAGGGATCCGCGGAGCGCTGGCCGACAGCATTTCTAACGGCCTGCTT
GATGAGCCCGTGCATATTCACTCGCACGGTGGTCGTGTGCATTTGATCCAAAGCGGTGAGCTGTACATCGACATGGCGTT
TATTGGCGTACCTTGTTGCGATGAATTTGGTAACGCTAACGGTTTTAAAGGCAAGTCCAATTGTGGCTCACTCGGCTATG
CCAAAGTTGATGCCGAGCACGCTGAAAAAGTGGTGATGCTGACCGAAGCGATCGTCGGTTTCCCGAATTTCCCTGCATCG
ATTACTCAAGATCGTGTTGATGCGGTGGTGCAAGTAGCCGAAGTGGGCGACCCAAGCAAAATCGGTGGGGATGCCACGCG
CATGACCACCAACCCACGCGAGCTGTTGATCGCCAAACGCGCTGCGGAAGTCATTGAGCATTCTGGCTATTTCTATGATG
GTTTTTCAATGCAGACTGGTTCGGGCGGTGCATCGCTCGCGGTTGCGCGCTTTTTGAAAGAGAAGATGCTGCGCCAAGAC
ATCCGTGCTTCTTTCGCTCTCGGTGGTATCACCGCAACCATGGTCGATATGCATGAGCAGGGGCTGATTGATTACTTGCT
CGATGTGCAGTGTTTCGATTCGGTGGCGGCGGGCTCGTTAGCGCGCAATCCGCATCATCTCGAAATCTCTGCTAACGAAT
ACGCCAACCCAAGCTCGAAAGGGGCGGCGGTGGATAGGCTGGATGTGGTTATCTTGAGTGCGCTGGAGATTGATACCCAG
TTTAACGTTAACGTGATCACCGGCTCTGATGGGGTAATTCGCGGTGCATCCGGCGGCCACTGCGACACGGCGGCTGCGGC
AAACTTATCGATTATTGTGGCGCCTTTGGTGCGTGGCCGCATTCCGACCGTGGTCGAGAAGGTCACTAACGTCATCACCC
CAGGATCCACCATTGATGTGTTGGTGACGGATCAAGGTATCGCGGTAAACCCGAATCGTCCCGAACTGAAAGCACGTTTC
ATCGAGGCGCAACTGCCCGTTGTCGAGATTGAAGCGTTGCAACAGCGTGCCGAGTTGCTGACGGGCAAACCCCAGCCGTT
GCAATTTGAAGACAAAACCGTTGCCTTTGTTCACTACCGTGACGGTTCGATCATTGATGTTATCAAACAGGTAAAAAGCC
TATGA

Upstream 100 bases:

>100_bases
ACCCAAAAAGAGGTCGATCACGCCATCGCAGTCATCGAAGCCGCCGAAGAAGCCGCAGCGAAAGGGCTAGGCGTGGTTTC
CCTTAACGGCAAAATGGTCG

Downstream 100 bases:

>100_bases
GTCACCATCCGGATCTTTCAGTCAGTCTGGACCAGCTCCTTCTGCGTAAAGAAGTGCGGGTTCGGCAACAAGGTGAGTGG
CTAAAGCGCCATTCACTGCC

Product: citrate lyase, alpha subunit

Products: NA

Alternate protein names: Citrase alpha chain; Citrate (pro-3S)-lyase alpha chain; Citrate CoA-transferase subunit [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MAPSSNAHVGHCNVLNPASNTKGHLMSINTLNNPLSVLEVERYALTPYTQARAITPHLHDEQAKKSRKVKSSIREAIVDL
GLKDGMTISFHHAFRGGDKTVNLVMEVIAELGIKNLTLASSSLTSCHSPLIEHIKNGVVSKIYTSGIRGALADSISNGLL
DEPVHIHSHGGRVHLIQSGELYIDMAFIGVPCCDEFGNANGFKGKSNCGSLGYAKVDAEHAEKVVMLTEAIVGFPNFPAS
ITQDRVDAVVQVAEVGDPSKIGGDATRMTTNPRELLIAKRAAEVIEHSGYFYDGFSMQTGSGGASLAVARFLKEKMLRQD
IRASFALGGITATMVDMHEQGLIDYLLDVQCFDSVAAGSLARNPHHLEISANEYANPSSKGAAVDRLDVVILSALEIDTQ
FNVNVITGSDGVIRGASGGHCDTAAAANLSIIVAPLVRGRIPTVVEKVTNVITPGSTIDVLVTDQGIAVNPNRPELKARF
IEAQLPVVEIEALQQRAELLTGKPQPLQFEDKTVAFVHYRDGSIIDVIKQVKSL

Sequences:

>Translated_534_residues
MAPSSNAHVGHCNVLNPASNTKGHLMSINTLNNPLSVLEVERYALTPYTQARAITPHLHDEQAKKSRKVKSSIREAIVDL
GLKDGMTISFHHAFRGGDKTVNLVMEVIAELGIKNLTLASSSLTSCHSPLIEHIKNGVVSKIYTSGIRGALADSISNGLL
DEPVHIHSHGGRVHLIQSGELYIDMAFIGVPCCDEFGNANGFKGKSNCGSLGYAKVDAEHAEKVVMLTEAIVGFPNFPAS
ITQDRVDAVVQVAEVGDPSKIGGDATRMTTNPRELLIAKRAAEVIEHSGYFYDGFSMQTGSGGASLAVARFLKEKMLRQD
IRASFALGGITATMVDMHEQGLIDYLLDVQCFDSVAAGSLARNPHHLEISANEYANPSSKGAAVDRLDVVILSALEIDTQ
FNVNVITGSDGVIRGASGGHCDTAAAANLSIIVAPLVRGRIPTVVEKVTNVITPGSTIDVLVTDQGIAVNPNRPELKARF
IEAQLPVVEIEALQQRAELLTGKPQPLQFEDKTVAFVHYRDGSIIDVIKQVKSL
>Mature_533_residues
APSSNAHVGHCNVLNPASNTKGHLMSINTLNNPLSVLEVERYALTPYTQARAITPHLHDEQAKKSRKVKSSIREAIVDLG
LKDGMTISFHHAFRGGDKTVNLVMEVIAELGIKNLTLASSSLTSCHSPLIEHIKNGVVSKIYTSGIRGALADSISNGLLD
EPVHIHSHGGRVHLIQSGELYIDMAFIGVPCCDEFGNANGFKGKSNCGSLGYAKVDAEHAEKVVMLTEAIVGFPNFPASI
TQDRVDAVVQVAEVGDPSKIGGDATRMTTNPRELLIAKRAAEVIEHSGYFYDGFSMQTGSGGASLAVARFLKEKMLRQDI
RASFALGGITATMVDMHEQGLIDYLLDVQCFDSVAAGSLARNPHHLEISANEYANPSSKGAAVDRLDVVILSALEIDTQF
NVNVITGSDGVIRGASGGHCDTAAAANLSIIVAPLVRGRIPTVVEKVTNVITPGSTIDVLVTDQGIAVNPNRPELKARFI
EAQLPVVEIEALQQRAELLTGKPQPLQFEDKTVAFVHYRDGSIIDVIKQVKSL

Specific function: Represents a citrate:acetyl-ACP transferase [H]

COG id: COG3051

COG function: function code C; Citrate lyase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786832, Length=473, Percent_Identity=71.2473572938689, Blast_Score=697, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006472 [H]

Pfam domain/function: PF04223 CitF [H]

EC number: =4.1.3.6; =2.8.3.10 [H]

Molecular weight: Translated: 57156; Mature: 57025

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPSSNAHVGHCNVLNPASNTKGHLMSINTLNNPLSVLEVERYALTPYTQARAITPHLHD
CCCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHEEEHHHCCCCCHHCCCCCCCCCH
EQAKKSRKVKSSIREAIVDLGLKDGMTISFHHAFRGGDKTVNLVMEVIAELGIKNLTLAS
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECC
SSLTSCHSPLIEHIKNGVVSKIYTSGIRGALADSISNGLLDEPVHIHSHGGRVHLIQSGE
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCC
LYIDMAFIGVPCCDEFGNANGFKGKSNCGSLGYAKVDAEHAEKVVMLTEAIVGFPNFPAS
EEEEEEEECCCCHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCH
ITQDRVDAVVQVAEVGDPSKIGGDATRMTTNPRELLIAKRAAEVIEHSGYFYDGFSMQTG
HHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCEEECEEEECC
SGGASLAVARFLKEKMLRQDIRASFALGGITATMVDMHEQGLIDYLLDVQCFDSVAAGSL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARNPHHLEISANEYANPSSKGAAVDRLDVVILSALEIDTQFNVNVITGSDGVIRGASGGH
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHEEECCEEEEEEEECCCCEEECCCCCC
CDTAAAANLSIIVAPLVRGRIPTVVEKVTNVITPGSTIDVLVTDQGIAVNPNRPELKARF
CCCHHHCCCEEEEEHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCHHHHHH
IEAQLPVVEIEALQQRAELLTGKPQPLQFEDKTVAFVHYRDGSIIDVIKQVKSL
HHHCCCCHHHHHHHHHHHHHCCCCCCCEECCCEEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
APSSNAHVGHCNVLNPASNTKGHLMSINTLNNPLSVLEVERYALTPYTQARAITPHLHD
CCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHEEEHHHCCCCCHHCCCCCCCCCH
EQAKKSRKVKSSIREAIVDLGLKDGMTISFHHAFRGGDKTVNLVMEVIAELGIKNLTLAS
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECC
SSLTSCHSPLIEHIKNGVVSKIYTSGIRGALADSISNGLLDEPVHIHSHGGRVHLIQSGE
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCC
LYIDMAFIGVPCCDEFGNANGFKGKSNCGSLGYAKVDAEHAEKVVMLTEAIVGFPNFPAS
EEEEEEEECCCCHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCH
ITQDRVDAVVQVAEVGDPSKIGGDATRMTTNPRELLIAKRAAEVIEHSGYFYDGFSMQTG
HHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCEEECEEEECC
SGGASLAVARFLKEKMLRQDIRASFALGGITATMVDMHEQGLIDYLLDVQCFDSVAAGSL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARNPHHLEISANEYANPSSKGAAVDRLDVVILSALEIDTQFNVNVITGSDGVIRGASGGH
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHEEECCEEEEEEEECCCCEEECCCCCC
CDTAAAANLSIIVAPLVRGRIPTVVEKVTNVITPGSTIDVLVTDQGIAVNPNRPELKARF
CCCHHHCCCEEEEEHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCHHHHHH
IEAQLPVVEIEALQQRAELLTGKPQPLQFEDKTVAFVHYRDGSIIDVIKQVKSL
HHHCCCCHHHHHHHHHHHHHCCCCCCCEECCCEEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]