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The map label for this gene is citB [H]

Identifier: 227080969

GI number: 227080969

Start: 803042

End: 803725

Strand: Reverse

Name: citB [H]

Synonym: VCM66_0748

Alternate gene names: 227080969

Gene position: 803725-803042 (Counterclockwise)

Preceding gene: 227080970

Following gene: 227080965

Centisome position: 27.79

GC content: 47.95

Gene sequence:

>684_bases
ATGATGGAATTAATCGACGTATTGATCGTAGAGGATGAAACGAGTATTGCTGACGTGCACAGCTTTTATCTCAAACAAAC
CGCCCGCTTTCGCCCAGTCGGCGTGGCTCAAAGTATCAACGAAGCGCGCAATATGGTGCGCATTCTCAAACCTAAACTGA
TTTTCTTGGATAACTACCTGCCTGATGGTCGTGGCATTGAGTTTTTGAAAGAGCTAACCCATCAACCGCAAGCGCCGGAT
GTCATTTTTATCACCGCCGCTTCGGATATGGAGACCGTGCGCGAAGCCGTACGCTGCGGAGTTTTTGATTACCTGTTAAA
ACCGATCGCCTACGATCGAGTACAAAACTCGCTGGAACGGTATCTGAAATACATCAGCTCGCTGCGCGCCAATGACAGTG
TGAATCAAAGACACGTCGATGAGCTGTTTAATTTTCAAGCCAAAACCGATCAGCTTGATGGGTTGCCGAAAGGGATTGAT
GAGCTGACCTTAATCAAAATCCAAGAGATTTATCACCATGAACCGCAAGCCGCCTACACCGCAGAATTGTTAGGACAAGC
GATTGGGATCAGCAAAACCACGGCCAGACGTTATTTGGAATATTGTGTGGCCAGTGGTTTTCTGGAAGCCTGTATTCAGC
ATGGTCGGGTGGGCCGTCCCGAACGACTTTATCAAAAGCGCTAA

Upstream 100 bases:

>100_bases
CAAGGGACTATTTTTTCTATTTTTATTCCCAATAGACCCAAAACGCTTGCGGCGGAAAACCTTCCAGCGCTTGCCGAACG
GGCATAAGAAGAGTTTGATT

Downstream 100 bases:

>100_bases
CCCACTCAGCATGCCGAATGCATCAGGCATGAGTTACAAAGTGTAGTGAAAGGGGATCACTTCGGTGCTCTCCCCTATTT
CCAACATTCCTTCTAAGTTG

Product: transcriptional regulator CitB

Products: NA

Alternate protein names: Destabilizer of plasmid inheritance [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MMELIDVLIVEDETSIADVHSFYLKQTARFRPVGVAQSINEARNMVRILKPKLIFLDNYLPDGRGIEFLKELTHQPQAPD
VIFITAASDMETVREAVRCGVFDYLLKPIAYDRVQNSLERYLKYISSLRANDSVNQRHVDELFNFQAKTDQLDGLPKGID
ELTLIKIQEIYHHEPQAAYTAELLGQAIGISKTTARRYLEYCVASGFLEACIQHGRVGRPERLYQKR

Sequences:

>Translated_227_residues
MMELIDVLIVEDETSIADVHSFYLKQTARFRPVGVAQSINEARNMVRILKPKLIFLDNYLPDGRGIEFLKELTHQPQAPD
VIFITAASDMETVREAVRCGVFDYLLKPIAYDRVQNSLERYLKYISSLRANDSVNQRHVDELFNFQAKTDQLDGLPKGID
ELTLIKIQEIYHHEPQAAYTAELLGQAIGISKTTARRYLEYCVASGFLEACIQHGRVGRPERLYQKR
>Mature_227_residues
MMELIDVLIVEDETSIADVHSFYLKQTARFRPVGVAQSINEARNMVRILKPKLIFLDNYLPDGRGIEFLKELTHQPQAPD
VIFITAASDMETVREAVRCGVFDYLLKPIAYDRVQNSLERYLKYISSLRANDSVNQRHVDELFNFQAKTDQLDGLPKGID
ELTLIKIQEIYHHEPQAAYTAELLGQAIGISKTTARRYLEYCVASGFLEACIQHGRVGRPERLYQKR

Specific function: Member of the two-component regulatory system DpiA/DpiB, which is essential for expression of citrate-specific fermentation genes and genes involved in plasmid inheritance. Could be involved in response to both the presence of citrate and external redox c

COG id: COG4565

COG function: function code KT; Response regulator of citrate/malate metabolism

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786838, Length=226, Percent_Identity=49.5575221238938, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI1790566, Length=220, Percent_Identity=27.2727272727273, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790437, Length=128, Percent_Identity=34.375, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1786599, Length=130, Percent_Identity=30.7692307692308, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 26033; Mature: 26033

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMELIDVLIVEDETSIADVHSFYLKQTARFRPVGVAQSINEARNMVRILKPKLIFLDNYL
CHHHHEEHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHEEEECCC
PDGRGIEFLKELTHQPQAPDVIFITAASDMETVREAVRCGVFDYLLKPIAYDRVQNSLER
CCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLKYISSLRANDSVNQRHVDELFNFQAKTDQLDGLPKGIDELTLIKIQEIYHHEPQAAYT
HHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHH
AELLGQAIGISKTTARRYLEYCVASGFLEACIQHGRVGRPERLYQKR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCC
>Mature Secondary Structure
MMELIDVLIVEDETSIADVHSFYLKQTARFRPVGVAQSINEARNMVRILKPKLIFLDNYL
CHHHHEEHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHEEEECCC
PDGRGIEFLKELTHQPQAPDVIFITAASDMETVREAVRCGVFDYLLKPIAYDRVQNSLER
CCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLKYISSLRANDSVNQRHVDELFNFQAKTDQLDGLPKGIDELTLIKIQEIYHHEPQAAYT
HHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHH
AELLGQAIGISKTTARRYLEYCVASGFLEACIQHGRVGRPERLYQKR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]