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The map label for this gene is citA [H]

Identifier: 227080970

GI number: 227080970

Start: 803739

End: 805436

Strand: Reverse

Name: citA [H]

Synonym: VCM66_0749

Alternate gene names: 227080970

Gene position: 805436-803739 (Counterclockwise)

Preceding gene: 227080971

Following gene: 227080969

Centisome position: 27.85

GC content: 49.76

Gene sequence:

>1698_bases
ATGAAAATATCACTAGCGCAATTTTTACATACCAAGGTCTGTCAGACTCTTTCATTTCAACAAAGGGTCGGCGCTCTTTT
GGTGGCTATGGTGGTGATCCAGCTTTCGCTGGTGGCTGGCTTTTTTCATCAAACCTTAAGTGAAACCTTACAAGATCAGA
TCAGCACCAAAGCGCTAATCCAAGCGCGCGAAATTGCGACCGATCCCAATCTCATTGTGCTGATCCAACAAAATCGGCTT
GCTGAAGTGCAGGCCAAAATCGATCGTTTACAACGCATCTCGGATGCCAATTTTATTGTGATTGGTGATGCCAATGGCAT
TCGCATCGCGCATCCGGATGAGCAAAAAATTGGTTTACCGATGCAAGGCGGTGACAGCCGTCGCGCATTAAAAGAGGGCG
AATATTACACCTCAACCCAAAAAGGCAGCCTAGGCTGGGCCATTCGTGGCAAAGCCGCGATTGTCGCCCCTTCGGGAGAG
ATCCTCGGCGTGGTTTCTGTTGGCTACCTGCTCGATAACATCAGCAGTTGGCTGCGGGTTTACTCCTACCCAGTGATTTT
CACTGTGCTGCTTTTAATGCTGCTTTCTGCGCTCGGCGCTTGGATCTTTACCCGCCATATCAAACAGCAGATGTTCAATA
TGGAGCCGGAAGAGATCGCCATGAACCTCAACCTACAGCAGTCGATTTTACAAAGTGTGTATGAAGGTATCGTCGCGATC
AGCTTGAAAGGGGAAATCCTGTCCGTCAATGCTAAGGCACTGAATATTTTGGGCATTGCCCATCAGCCGACTCATTTGAT
TGGGCGTAATGTTCAAGAGTTCATCACTCCAACCTGCTTTTTTATGGGCGCAAGCCCGTTTGGCAAGTTGGCGCAGCAAA
ACCGTGTCAGCCAGCAAGATGAACTGATCAGCTGTAATGGTGAAACCTTGGTCGCGAATCGGGTACCGATCAACTCTGGG
CAGCAACAAATCGGCTGGGTGGTCAGCTTTCGTCGCCGCAATGATTTCAACACACTCACCTCGCAGCTCACTCAGATCCG
TCAACATAACGATAATCTGCGCGTGATGAGTCACGAGTTTGCCAACCGCCTCTCAACCATCGGCGGCTTAATTCAAATCG
GTGCGTATGACGAAGCGGTGAAAACCATTCGCCGCGAAACGGCAGAGCAGCAACAGCTGATCGATTTCATCGCCCAAACT
TTTCACCCGAAAGTGATCGCGGGGTTACTGCTGGGAAAATACAGCCGCGCCAAAGAGTTAGGACTGTGTTTAGAGTTCGA
TCCGCTATCGCATCTGCACCAAGAGCCGCAGTGCATGACTTCAGATGAACTGGCGGCGGTACTTGGCAATCTGCTGGATA
ACGCGTTTGAAGCCACGCTAAAAAATCCACACAGCAATAAAACCATTTCCCTGCTGCTGACCGATAACGGTGCGGAGTTG
GTGATTGAAGTGGCGGACAACGGCATCGGGATCTCGGCCGACATCGCCCAAACTTTGTTTCTCAAGGGAGTGAGTAGCAA
AAATCAAGAAGGACACGGTATCGGACTTTACCTTGTCCATCAATTTGTCACTCAGGCTCATGGATCGATTCTTATCGACA
GTGCGGAGCCGCAAGGGACTATTTTTTCTATTTTTATTCCCAATAGACCCAAAACGCTTGCGGCGGAAAACCTTCCAGCG
CTTGCCGAACGGGCATAA

Upstream 100 bases:

>100_bases
GGGGTAATGATCAAATATGTGATGGGCTAAACATAAATGGTTGGGGTTTCTTAGACCCCAATCAATAAGCCCCGTTAATA
GCAAGGATAGACTCGCCGGC

Downstream 100 bases:

>100_bases
GAAGAGTTTGATTATGATGGAATTAATCGACGTATTGATCGTAGAGGATGAAACGAGTATTGCTGACGTGCACAGCTTTT
ATCTCAAACAAACCGCCCGC

Product: sensor kinase citA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MKISLAQFLHTKVCQTLSFQQRVGALLVAMVVIQLSLVAGFFHQTLSETLQDQISTKALIQAREIATDPNLIVLIQQNRL
AEVQAKIDRLQRISDANFIVIGDANGIRIAHPDEQKIGLPMQGGDSRRALKEGEYYTSTQKGSLGWAIRGKAAIVAPSGE
ILGVVSVGYLLDNISSWLRVYSYPVIFTVLLLMLLSALGAWIFTRHIKQQMFNMEPEEIAMNLNLQQSILQSVYEGIVAI
SLKGEILSVNAKALNILGIAHQPTHLIGRNVQEFITPTCFFMGASPFGKLAQQNRVSQQDELISCNGETLVANRVPINSG
QQQIGWVVSFRRRNDFNTLTSQLTQIRQHNDNLRVMSHEFANRLSTIGGLIQIGAYDEAVKTIRRETAEQQQLIDFIAQT
FHPKVIAGLLLGKYSRAKELGLCLEFDPLSHLHQEPQCMTSDELAAVLGNLLDNAFEATLKNPHSNKTISLLLTDNGAEL
VIEVADNGIGISADIAQTLFLKGVSSKNQEGHGIGLYLVHQFVTQAHGSILIDSAEPQGTIFSIFIPNRPKTLAAENLPA
LAERA

Sequences:

>Translated_565_residues
MKISLAQFLHTKVCQTLSFQQRVGALLVAMVVIQLSLVAGFFHQTLSETLQDQISTKALIQAREIATDPNLIVLIQQNRL
AEVQAKIDRLQRISDANFIVIGDANGIRIAHPDEQKIGLPMQGGDSRRALKEGEYYTSTQKGSLGWAIRGKAAIVAPSGE
ILGVVSVGYLLDNISSWLRVYSYPVIFTVLLLMLLSALGAWIFTRHIKQQMFNMEPEEIAMNLNLQQSILQSVYEGIVAI
SLKGEILSVNAKALNILGIAHQPTHLIGRNVQEFITPTCFFMGASPFGKLAQQNRVSQQDELISCNGETLVANRVPINSG
QQQIGWVVSFRRRNDFNTLTSQLTQIRQHNDNLRVMSHEFANRLSTIGGLIQIGAYDEAVKTIRRETAEQQQLIDFIAQT
FHPKVIAGLLLGKYSRAKELGLCLEFDPLSHLHQEPQCMTSDELAAVLGNLLDNAFEATLKNPHSNKTISLLLTDNGAEL
VIEVADNGIGISADIAQTLFLKGVSSKNQEGHGIGLYLVHQFVTQAHGSILIDSAEPQGTIFSIFIPNRPKTLAAENLPA
LAERA
>Mature_565_residues
MKISLAQFLHTKVCQTLSFQQRVGALLVAMVVIQLSLVAGFFHQTLSETLQDQISTKALIQAREIATDPNLIVLIQQNRL
AEVQAKIDRLQRISDANFIVIGDANGIRIAHPDEQKIGLPMQGGDSRRALKEGEYYTSTQKGSLGWAIRGKAAIVAPSGE
ILGVVSVGYLLDNISSWLRVYSYPVIFTVLLLMLLSALGAWIFTRHIKQQMFNMEPEEIAMNLNLQQSILQSVYEGIVAI
SLKGEILSVNAKALNILGIAHQPTHLIGRNVQEFITPTCFFMGASPFGKLAQQNRVSQQDELISCNGETLVANRVPINSG
QQQIGWVVSFRRRNDFNTLTSQLTQIRQHNDNLRVMSHEFANRLSTIGGLIQIGAYDEAVKTIRRETAEQQQLIDFIAQT
FHPKVIAGLLLGKYSRAKELGLCLEFDPLSHLHQEPQCMTSDELAAVLGNLLDNAFEATLKNPHSNKTISLLLTDNGAEL
VIEVADNGIGISADIAQTLFLKGVSSKNQEGHGIGLYLVHQFVTQAHGSILIDSAEPQGTIFSIFIPNRPKTLAAENLPA
LAERA

Specific function: Member of the two-component regulatory system CitA/CitB. Probably activates CitB by phosphorylation. The periplasmic domain binds H-citrate(2-), which is essential for induction of the citrate-fermentation genes [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=540, Percent_Identity=38.5185185185185, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1790567, Length=530, Percent_Identity=30.9433962264151, Blast_Score=214, Evalue=9e-57,
Organism=Escherichia coli, GI1790436, Length=121, Percent_Identity=34.7107438016529, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 62166; Mature: 62166

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISLAQFLHTKVCQTLSFQQRVGALLVAMVVIQLSLVAGFFHQTLSETLQDQISTKALI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAREIATDPNLIVLIQQNRLAEVQAKIDRLQRISDANFIVIGDANGIRIAHPDEQKIGLP
HHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCC
MQGGDSRRALKEGEYYTSTQKGSLGWAIRGKAAIVAPSGEILGVVSVGYLLDNISSWLRV
CCCCCHHHHHHCCCCEECCCCCCCCEEEECCEEEEECCCCEEHHHHHHHHHHHHHHHHHH
YSYPVIFTVLLLMLLSALGAWIFTRHIKQQMFNMEPEEIAMNLNLQQSILQSVYEGIVAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEECCCHHHHHHHHHHHCEEEE
SLKGEILSVNAKALNILGIAHQPTHLIGRNVQEFITPTCFFMGASPFGKLAQQNRVSQQD
EECCCEEEECHHEEEEEEECCCCHHHHHCCHHHHHCCHHEEECCCHHHHHHHHHCCCCCC
ELISCNGETLVANRVPINSGQQQIGWVVSFRRRNDFNTLTSQLTQIRQHNDNLRVMSHEF
CEEECCCCEEEEECCCCCCCCHHHHHHEEEHHCCCHHHHHHHHHHHHHCCCCCEEHHHHH
ANRLSTIGGLIQIGAYDEAVKTIRRETAEQQQLIDFIAQTFHPKVIAGLLLGKYSRAKEL
HHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHC
GLCLEFDPLSHLHQEPQCMTSDELAAVLGNLLDNAFEATLKNPHSNKTISLLLTDNGAEL
CCEEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEE
VIEVADNGIGISADIAQTLFLKGVSSKNQEGHGIGLYLVHQFVTQAHGSILIDSAEPQGT
EEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCC
IFSIFIPNRPKTLAAENLPALAERA
EEEEEECCCCCCHHHCCCCHHHCCC
>Mature Secondary Structure
MKISLAQFLHTKVCQTLSFQQRVGALLVAMVVIQLSLVAGFFHQTLSETLQDQISTKALI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAREIATDPNLIVLIQQNRLAEVQAKIDRLQRISDANFIVIGDANGIRIAHPDEQKIGLP
HHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCC
MQGGDSRRALKEGEYYTSTQKGSLGWAIRGKAAIVAPSGEILGVVSVGYLLDNISSWLRV
CCCCCHHHHHHCCCCEECCCCCCCCEEEECCEEEEECCCCEEHHHHHHHHHHHHHHHHHH
YSYPVIFTVLLLMLLSALGAWIFTRHIKQQMFNMEPEEIAMNLNLQQSILQSVYEGIVAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEECCCHHHHHHHHHHHCEEEE
SLKGEILSVNAKALNILGIAHQPTHLIGRNVQEFITPTCFFMGASPFGKLAQQNRVSQQD
EECCCEEEECHHEEEEEEECCCCHHHHHCCHHHHHCCHHEEECCCHHHHHHHHHCCCCCC
ELISCNGETLVANRVPINSGQQQIGWVVSFRRRNDFNTLTSQLTQIRQHNDNLRVMSHEF
CEEECCCCEEEEECCCCCCCCHHHHHHEEEHHCCCHHHHHHHHHHHHHCCCCCEEHHHHH
ANRLSTIGGLIQIGAYDEAVKTIRRETAEQQQLIDFIAQTFHPKVIAGLLLGKYSRAKEL
HHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHC
GLCLEFDPLSHLHQEPQCMTSDELAAVLGNLLDNAFEATLKNPHSNKTISLLLTDNGAEL
CCEEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEE
VIEVADNGIGISADIAQTLFLKGVSSKNQEGHGIGLYLVHQFVTQAHGSILIDSAEPQGT
EEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCC
IFSIFIPNRPKTLAAENLPALAERA
EEEEEECCCCCCHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8748036; 10447894 [H]