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The map label for this gene is pheA [H]

Identifier: 227080885

GI number: 227080885

Start: 712277

End: 713452

Strand: Reverse

Name: pheA [H]

Synonym: VCM66_0663

Alternate gene names: 227080885

Gene position: 713452-712277 (Counterclockwise)

Preceding gene: 227080886

Following gene: 227080884

Centisome position: 24.67

GC content: 47.36

Gene sequence:

>1176_bases
ATGACAGACAAACAATACTCACTCGACGATATTCGCTTACGGCTAAACGAACTCGACGATCAACTGCTAAACCTGCTGTC
AGAACGACGCAAAATGAGTATTGAGGTCGCCAAAAGCAAAGTCGAAACCGCGAAACCTGTACGTGATCCGGCTCGTGAAC
AGCAGCTACTGGTAAAACTCATCAATGCTGGCAAAGAGAAATATCAGCTCGATCCTCAATACATCACCAAAATTTTCCAC
ACCATCATTGAAGATTCGGTTTTGCTTCAGCAATCCTATCTGCAAAACCTTGCGAATCCACAAAGTCGTAAGCCATTGGC
TAGAGTCGCCTTTTTAGGCGCTAAAGGCTCTTATTCACATCTGGCGACTCGCGAGTATTTCAGCCGCAAAAATACAGAGC
TGATTGAGCTCAACTGCGACCATTTTAAAGAGGTGGCAAGAACCGTGGAATCCGGCCATGCGGATTATGGTGTGCTACCG
ATTGAAAACACCAGCTCAGGCTCCATCAACGAAGTTTACGATTTGCTGCAGCACACCACACTGTACATAGTGGGTGAGTT
AACGCAGCCAATAGAGCATTGCCTGGTGGCCACGCAAGAGATTCGTTTGGAGGATCTGAAAGTCCTCTATTCCCATCCAC
AGCCTCACCAGCAGTGCAGCGAATTTCTTAGCCGCTTAAAAGGGGTCAAGTTAGAAAGTTGCGCCAGTACTGCAGATGCC
ATGAAAAAAGTGCAAGAGCTCAATCGTGCGGATGTAGCAGCGATTGGCAACTCAGCCAGTGGAAAGCTGTACGGACTGCA
ACCGATTCAAGGAAATATTGCCAACCAAACCGAAAATCACACTCGCTTTATCGTGGTGGCTCGTAAACCCGTCGACGTTT
CACCACAAATTCCAGCCAAAACCACCTTGATTATGTCAACTTCACAAGAGGCGGGCTCGCTGGTTTCGACCTTACTCGTG
CTGCAACGTTACGGCATTAATATGACTAAGCTGGAATCGCGTCCGATTATGGGTAATCCGTGGGAAGAAATGTTCTACGT
AGATTTAGAAGCGCACATTGACTCCGATGAGATGCAGCAAGCGTTGGCAGAACTCACTCAACTGACTCGACACCTTAAAG
TGCTTGGCTGCTACCCTAGTGAAAACGTCAAACCCACTCAGGTGAAATTCATTTAG

Upstream 100 bases:

>100_bases
TTCTCCAATAATGTTTGGAGGCTCGCTCGTTGTTCAAAGACAAGTCAAAAACGAACAGAAAGCCTCCTCTTAAGGGGGCT
TTTTTTATAAGGATAACTTT

Downstream 100 bases:

>100_bases
CGTCAATAAATTGAACCTTTTAAGCTGACAGCTTCACCCGCTCTGATTATAATGCGACCAAATTATACATAGAGTTAGGT
GATCCAGGCTGTCAGCGTAG

Product: chorismate mutase/prephenate dehydratase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTDKQYSLDDIRLRLNELDDQLLNLLSERRKMSIEVAKSKVETAKPVRDPAREQQLLVKLINAGKEKYQLDPQYITKIFH
TIIEDSVLLQQSYLQNLANPQSRKPLARVAFLGAKGSYSHLATREYFSRKNTELIELNCDHFKEVARTVESGHADYGVLP
IENTSSGSINEVYDLLQHTTLYIVGELTQPIEHCLVATQEIRLEDLKVLYSHPQPHQQCSEFLSRLKGVKLESCASTADA
MKKVQELNRADVAAIGNSASGKLYGLQPIQGNIANQTENHTRFIVVARKPVDVSPQIPAKTTLIMSTSQEAGSLVSTLLV
LQRYGINMTKLESRPIMGNPWEEMFYVDLEAHIDSDEMQQALAELTQLTRHLKVLGCYPSENVKPTQVKFI

Sequences:

>Translated_391_residues
MTDKQYSLDDIRLRLNELDDQLLNLLSERRKMSIEVAKSKVETAKPVRDPAREQQLLVKLINAGKEKYQLDPQYITKIFH
TIIEDSVLLQQSYLQNLANPQSRKPLARVAFLGAKGSYSHLATREYFSRKNTELIELNCDHFKEVARTVESGHADYGVLP
IENTSSGSINEVYDLLQHTTLYIVGELTQPIEHCLVATQEIRLEDLKVLYSHPQPHQQCSEFLSRLKGVKLESCASTADA
MKKVQELNRADVAAIGNSASGKLYGLQPIQGNIANQTENHTRFIVVARKPVDVSPQIPAKTTLIMSTSQEAGSLVSTLLV
LQRYGINMTKLESRPIMGNPWEEMFYVDLEAHIDSDEMQQALAELTQLTRHLKVLGCYPSENVKPTQVKFI
>Mature_390_residues
TDKQYSLDDIRLRLNELDDQLLNLLSERRKMSIEVAKSKVETAKPVRDPAREQQLLVKLINAGKEKYQLDPQYITKIFHT
IIEDSVLLQQSYLQNLANPQSRKPLARVAFLGAKGSYSHLATREYFSRKNTELIELNCDHFKEVARTVESGHADYGVLPI
ENTSSGSINEVYDLLQHTTLYIVGELTQPIEHCLVATQEIRLEDLKVLYSHPQPHQQCSEFLSRLKGVKLESCASTADAM
KKVQELNRADVAAIGNSASGKLYGLQPIQGNIANQTENHTRFIVVARKPVDVSPQIPAKTTLIMSTSQEAGSLVSTLLVL
QRYGINMTKLESRPIMGNPWEEMFYVDLEAHIDSDEMQQALAELTQLTRHLKVLGCYPSENVKPTQVKFI

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=378, Percent_Identity=55.026455026455, Blast_Score=422, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6324013, Length=323, Percent_Identity=22.6006191950464, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010952
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 44131; Mature: 44000

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00857 PREPHENATE_DEHYDR_1 ; PS00858 PREPHENATE_DEHYDR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKQYSLDDIRLRLNELDDQLLNLLSERRKMSIEVAKSKVETAKPVRDPAREQQLLVKL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
INAGKEKYQLDPQYITKIFHTIIEDSVLLQQSYLQNLANPQSRKPLARVAFLGAKGSYSH
HHCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCHHH
LATREYFSRKNTELIELNCDHFKEVARTVESGHADYGVLPIENTSSGSINEVYDLLQHTT
HHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
LYIVGELTQPIEHCLVATQEIRLEDLKVLYSHPQPHQQCSEFLSRLKGVKLESCASTADA
HEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
MKKVQELNRADVAAIGNSASGKLYGLQPIQGNIANQTENHTRFIVVARKPVDVSPQIPAK
HHHHHHHCHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCC
TTLIMSTSQEAGSLVSTLLVLQRYGINMTKLESRPIMGNPWEEMFYVDLEAHIDSDEMQQ
EEEEEECCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHEEEEEEHHCCCHHHHHH
ALAELTQLTRHLKVLGCYPSENVKPTQVKFI
HHHHHHHHHHHHHEEECCCCCCCCCCEEEEC
>Mature Secondary Structure 
TDKQYSLDDIRLRLNELDDQLLNLLSERRKMSIEVAKSKVETAKPVRDPAREQQLLVKL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
INAGKEKYQLDPQYITKIFHTIIEDSVLLQQSYLQNLANPQSRKPLARVAFLGAKGSYSH
HHCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCHHH
LATREYFSRKNTELIELNCDHFKEVARTVESGHADYGVLPIENTSSGSINEVYDLLQHTT
HHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
LYIVGELTQPIEHCLVATQEIRLEDLKVLYSHPQPHQQCSEFLSRLKGVKLESCASTADA
HEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
MKKVQELNRADVAAIGNSASGKLYGLQPIQGNIANQTENHTRFIVVARKPVDVSPQIPAK
HHHHHHHCHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCC
TTLIMSTSQEAGSLVSTLLVLQRYGINMTKLESRPIMGNPWEEMFYVDLEAHIDSDEMQQ
EEEEEECCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHEEEEEEHHCCCHHHHHH
ALAELTQLTRHLKVLGCYPSENVKPTQVKFI
HHHHHHHHHHHHHEEECCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1512561; 1444388 [H]