Click here to switch to the map view.

The map label for this gene is cysI [H]

Identifier: 227080597

GI number: 227080597

Start: 383447

End: 385180

Strand: Direct

Name: cysI [H]

Synonym: VCM66_0370

Alternate gene names: 227080597

Gene position: 383447-385180 (Clockwise)

Preceding gene: 227080596

Following gene: 227080598

Centisome position: 13.26

GC content: 49.19

Gene sequence:

>1734_bases
ATGAGCGCAAATCAAAACCCATCAGTACAAGAGGTGCTTGGCGAAGTGCTCGGCCCATGGTCGGATAATGAACGCCTAAA
GCGCGAGAGTCATTTTCTACGTGGGACGATCGAGCAAGATCTGCAAGATCGCATTACCGGCGGCTTTACCGCGGATAACT
TCCAGCTCATCCGCTTTCATGGCATGTATCAGCAAGATGATCGTGATATCCGTGCTGAGCGTAGCAAGCAGAAGCTTGAG
CCTTTGCATAATGTCATGCTCCGTGCGCGTATGCCGGGAGGGATCATCACTCCTCACCAGTGGCTCGCGATCGATAAGTT
TGCAACTGAACATACTCTGTACGGCAGTATCCGTTTAACCACACGACAAACGTTTCAGTTTCACGGTGTGCTTAAGCCGA
ATATCAAACTGATGCATCAAACCTTAAACAGCATTGGTATCGATTCGATTGCGACCGCAGGGGATGTGAACCGTAACGTA
TTGTGCACCTCTAACCCCGTGGAATCTGAACTGCATCTGCAAGCCTACGAGTGGGCAAAAAAGATCAGTGAACACCTACT
ACCGAAAACACGCGCTTATGCCGAAATTTGGTTGGATGGCGAAAAGATCGAAGGACCCGATGAAGAGCCCATTTTAGGTT
CAAACTATTTGCCGCGTAAGTTCAAAACGACCGTTGTGATCCCGCCACACAATGATGTGGATGTGCACGCCAATGACTTG
AACTTTGTCGCGATTGGTGAAAATGGCCAGTTGGTCGGTTTTAACGTGTTGGTGGGTGGGGGCCTTGCGATGACGCACGG
CGATACTTCAACCTATCCGCGTCGTGCCGATGACTTTGGTTTTATTCCTCTGGAAAAAACCTTGGAGGTGGCGGCTGCAG
TCGTGAGCACGCAACGCGATTGGGGGAACCGTTCTAACCGTAAAAATGCCAAAACCAAGTACACCTTAGATCGTGTCGGG
GTTGAGGTATTTAAAGCCGAAGTTGAAAAACGCGCGGGAATTACGTTTGCACCAAGCCGAGCTTATGAGTTTACCAGCCG
TGGCGATCGGATTGGTTGGGTTGAAGGCATCGATGGCAAACATCATTTAACCCTGTTTATTGAAAATGGCCGCATATTGG
ATTTTCCGGGTAAGCCACTGAAAACGGGTGTTGCTGAAATTGCTAAGGTGCATCAAGGCGATTTCCGGATGACGGCTAAC
CAGAACTTGATTGTGGCTGGTGTGCCAGCGGATCAGAAACCGCACATTGAGCAGCTCGCACGTGAACATGGCTTGATTGA
TGATGGTGTGAGTGAGCAGCGTATCAATTCTATGGCGTGTGTCGCCTTTCCAACTTGTCCATTGGCGATGGCGGAAGCCG
AGCGTTTTCTACCTTCTTTTGTCACAGAAGTGGAAGGTATTTTGGCAAAACATGCGTTACCAAAAGAAGAGAACATCATT
TTGCGTGTGACGGGCTGCCCGAATGGGTGCGGTCGAGCCATGTTGGCTGAGATTGGCCTAGTCGGTAAAGCACCGGGTCG
CTACAACCTCCATTTAGGCGGCAATCGAAATGGAACGCGTATTCCTAAGATGTATAAAGAGAACATTACCGATACGCAAA
TTCTTCAAGAAATTGATGAGTTGGTGGGACGTTGGGCTAGCGAGCGTCTGGACGGTGAAGGATTTGGTGATTTCACTATT
CGTGCAGGCATTATCGAAGAGGTAATCATCTCGAAGAGGGATTTCTATGCCTAA

Upstream 100 bases:

>100_bases
TTACTGTTGTCGAGCAACAAGGTGGCTTGAATCGTGAGCAAGCTGAAGAGTATGTGAGTGAACTGCGTAAAGCAAAACGT
TATCAAAGGGATGTCTACTA

Downstream 100 bases:

>100_bases
TCGTACCGTTCCAACACTAGAAGAGTTACTAACGTTAAATAAGGTGCAGCAAACGCTGCGCCTAACGGAAGTCAATCAGC
ATCTGGAATCCCTCACTGCG

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP [H]

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MSANQNPSVQEVLGEVLGPWSDNERLKRESHFLRGTIEQDLQDRITGGFTADNFQLIRFHGMYQQDDRDIRAERSKQKLE
PLHNVMLRARMPGGIITPHQWLAIDKFATEHTLYGSIRLTTRQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNV
LCTSNPVESELHLQAYEWAKKISEHLLPKTRAYAEIWLDGEKIEGPDEEPILGSNYLPRKFKTTVVIPPHNDVDVHANDL
NFVAIGENGQLVGFNVLVGGGLAMTHGDTSTYPRRADDFGFIPLEKTLEVAAAVVSTQRDWGNRSNRKNAKTKYTLDRVG
VEVFKAEVEKRAGITFAPSRAYEFTSRGDRIGWVEGIDGKHHLTLFIENGRILDFPGKPLKTGVAEIAKVHQGDFRMTAN
QNLIVAGVPADQKPHIEQLAREHGLIDDGVSEQRINSMACVAFPTCPLAMAEAERFLPSFVTEVEGILAKHALPKEENII
LRVTGCPNGCGRAMLAEIGLVGKAPGRYNLHLGGNRNGTRIPKMYKENITDTQILQEIDELVGRWASERLDGEGFGDFTI
RAGIIEEVIISKRDFYA

Sequences:

>Translated_577_residues
MSANQNPSVQEVLGEVLGPWSDNERLKRESHFLRGTIEQDLQDRITGGFTADNFQLIRFHGMYQQDDRDIRAERSKQKLE
PLHNVMLRARMPGGIITPHQWLAIDKFATEHTLYGSIRLTTRQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNV
LCTSNPVESELHLQAYEWAKKISEHLLPKTRAYAEIWLDGEKIEGPDEEPILGSNYLPRKFKTTVVIPPHNDVDVHANDL
NFVAIGENGQLVGFNVLVGGGLAMTHGDTSTYPRRADDFGFIPLEKTLEVAAAVVSTQRDWGNRSNRKNAKTKYTLDRVG
VEVFKAEVEKRAGITFAPSRAYEFTSRGDRIGWVEGIDGKHHLTLFIENGRILDFPGKPLKTGVAEIAKVHQGDFRMTAN
QNLIVAGVPADQKPHIEQLAREHGLIDDGVSEQRINSMACVAFPTCPLAMAEAERFLPSFVTEVEGILAKHALPKEENII
LRVTGCPNGCGRAMLAEIGLVGKAPGRYNLHLGGNRNGTRIPKMYKENITDTQILQEIDELVGRWASERLDGEGFGDFTI
RAGIIEEVIISKRDFYA
>Mature_576_residues
SANQNPSVQEVLGEVLGPWSDNERLKRESHFLRGTIEQDLQDRITGGFTADNFQLIRFHGMYQQDDRDIRAERSKQKLEP
LHNVMLRARMPGGIITPHQWLAIDKFATEHTLYGSIRLTTRQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVL
CTSNPVESELHLQAYEWAKKISEHLLPKTRAYAEIWLDGEKIEGPDEEPILGSNYLPRKFKTTVVIPPHNDVDVHANDLN
FVAIGENGQLVGFNVLVGGGLAMTHGDTSTYPRRADDFGFIPLEKTLEVAAAVVSTQRDWGNRSNRKNAKTKYTLDRVGV
EVFKAEVEKRAGITFAPSRAYEFTSRGDRIGWVEGIDGKHHLTLFIENGRILDFPGKPLKTGVAEIAKVHQGDFRMTANQ
NLIVAGVPADQKPHIEQLAREHGLIDDGVSEQRINSMACVAFPTCPLAMAEAERFLPSFVTEVEGILAKHALPKEENIIL
RVTGCPNGCGRAMLAEIGLVGKAPGRYNLHLGGNRNGTRIPKMYKENITDTQILQEIDELVGRWASERLDGEGFGDFTIR
AGIIEEVIISKRDFYA

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate [H]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=561, Percent_Identity=71.6577540106952, Blast_Score=864, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322597, Length=578, Percent_Identity=45.5017301038062, Blast_Score=513, Evalue=1e-146,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 64379; Mature: 64248

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANQNPSVQEVLGEVLGPWSDNERLKRESHFLRGTIEQDLQDRITGGFTADNFQLIRFH
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
GMYQQDDRDIRAERSKQKLEPLHNVMLRARMPGGIITPHQWLAIDKFATEHTLYGSIRLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHCCEEEEEEEEEE
TRQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVLCTSNPVESELHLQAYEWAK
EEEEEEEECEECCCHHHHHHHHHHCCCCHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHH
KISEHLLPKTRAYAEIWLDGEKIEGPDEEPILGSNYLPRKFKTTVVIPPHNDVDVHANDL
HHHHHHCCCHHCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCE
NFVAIGENGQLVGFNVLVGGGLAMTHGDTSTYPRRADDFGFIPLEKTLEVAAAVVSTQRD
EEEEECCCCCEEEEEEEEECCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
WGNRSNRKNAKTKYTLDRVGVEVFKAEVEKRAGITFAPSRAYEFTSRGDRIGWVEGIDGK
CCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCEECCCCCEECCCCCCEEEEEECCCCC
HHLTLFIENGRILDFPGKPLKTGVAEIAKVHQGDFRMTANQNLIVAGVPADQKPHIEQLA
EEEEEEEECCEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCHHHHHHH
REHGLIDDGVSEQRINSMACVAFPTCPLAMAEAERFLPSFVTEVEGILAKHALPKEENII
HHCCCCCCCCCHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LRVTGCPNGCGRAMLAEIGLVGKAPGRYNLHLGGNRNGTRIPKMYKENITDTQILQEIDE
EEEECCCCCHHHHHHHHHCCEECCCCEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHH
LVGRWASERLDGEGFGDFTIRAGIIEEVIISKRDFYA
HHHHHHHHCCCCCCCCCEEEEHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SANQNPSVQEVLGEVLGPWSDNERLKRESHFLRGTIEQDLQDRITGGFTADNFQLIRFH
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
GMYQQDDRDIRAERSKQKLEPLHNVMLRARMPGGIITPHQWLAIDKFATEHTLYGSIRLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHCCEEEEEEEEEE
TRQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVLCTSNPVESELHLQAYEWAK
EEEEEEEECEECCCHHHHHHHHHHCCCCHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHH
KISEHLLPKTRAYAEIWLDGEKIEGPDEEPILGSNYLPRKFKTTVVIPPHNDVDVHANDL
HHHHHHCCCHHCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCE
NFVAIGENGQLVGFNVLVGGGLAMTHGDTSTYPRRADDFGFIPLEKTLEVAAAVVSTQRD
EEEEECCCCCEEEEEEEEECCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
WGNRSNRKNAKTKYTLDRVGVEVFKAEVEKRAGITFAPSRAYEFTSRGDRIGWVEGIDGK
CCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCEECCCCCEECCCCCCEEEEEECCCCC
HHLTLFIENGRILDFPGKPLKTGVAEIAKVHQGDFRMTANQNLIVAGVPADQKPHIEQLA
EEEEEEEECCEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCHHHHHHH
REHGLIDDGVSEQRINSMACVAFPTCPLAMAEAERFLPSFVTEVEGILAKHALPKEENII
HHCCCCCCCCCHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LRVTGCPNGCGRAMLAEIGLVGKAPGRYNLHLGGNRNGTRIPKMYKENITDTQILQEIDE
EEEECCCCCHHHHHHHHHCCEECCCCEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHH
LVGRWASERLDGEGFGDFTIRAGIIEEVIISKRDFYA
HHHHHHHHCCCCCCCCCEEEEHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA