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The map label for this gene is cysJ [H]

Identifier: 227080596

GI number: 227080596

Start: 381603

End: 383447

Strand: Direct

Name: cysJ [H]

Synonym: VCM66_0369

Alternate gene names: 227080596

Gene position: 381603-383447 (Clockwise)

Preceding gene: 227080595

Following gene: 227080597

Centisome position: 13.19

GC content: 48.67

Gene sequence:

>1845_bases
ATGAATAGGGAAGTCGTGACGATGTCTACAGGAAACACTTTACCCCCGGCGCTAGCGGCACTCGCTAGTCCGTTAAATGA
TGCACAGCTCAATCAACTCCAACAAACCGTAACCCAACTGAATGCTCAGCAATTAGCTTGGGTCAGTGGTTATTTCTGGG
GATTGAGTCAATCCAATGCATTGAGCGTTCCACACATTTCCGCTGGGCAGACTGCGTCTGCTGCTTCGGGTAAGCTGACG
ATCATTTTCGCTTCGCAAACGGGAAATGCGAAAGGCGTTGCTCAGGCGTTATTGAAAGAAGCGCAAGCTGCAGGCATTCA
GGCCCAGTTATTTGACGCGAGCGATTACAAAGGCAAAGACTTAGCCAAAGAAACCCATGTAATTTTTGTCGCCTCAACCA
ATGGTGAAGGTGAAGCGCCGGATAATGCACTGGCACTGCATGAGTTTCTCAAATCCAAAAAAGCCCCCAAGCTGCCTAAT
CTTAAATATGGCGTCTTAGGATTGGGGGATTCCAGTTATCAGTTTTTCTGCCAAACCGGCAAAGACTTTGATCAGTTTTT
AGAAAACCTTGGGGCGCAACGTCTGGTTGAGCGCTTAGATGCGGATGTCGATTATCAGGCTGCAGCGACCGAGTGGCGCA
AGCAAGTCCTGTCTATTTTGAAAGATGAGCTGACAGGAGCCGCGGCAGTGACGAGCGTGGCTACTTTCGCAGTGAGCCAG
ACAGCTGAGAGTCACTACAGTAAAGAGCAACCTTACACGGCGAGCTTATCGACGAGCCAAAAAATTACTGGCCGTGATTC
AGGCAAAGATGTACGCCACATCGAAATTGATTTGGCCGATTCTGGCATCACATACCAACCGGGTGATGCATTAGGTGTGT
GGTATGAAAACCGTCCACAGCTGGTTAATGCCCTACTCGATAGCGTAGGACTTTCTGGTCATGAAGAAGTACAAGTGGAT
GGCGAAACGCTTTCTCTGCATTCGGCACTGACTCATCATTATGAAATTACTGCAGCTAATCCGCAGTTGGTGGCGCAATT
TGCTGAGCTTGCACAAAGCGAAAAGCTCACCTCTTTAGCCCAAGATAAAGAGGCACTGCGTGAATACGCGACGCGTACGC
AAGTGATTGATGTGTTGCGCGAAGAAAAAGTCACCCTTTCCGCGATTCAACTGTTATCACTTCTCCGTCGTTTAACGCCG
CGTCTTTATTCGATTGCCTCTAGTCAAAGCGAGGTGGGGGAAGAGGTGCATCTGACGGTTGGGGTGGTCGAGTATGAGTA
TGAGGGAGAGCAACGCTTGGGTGGTGCATCCAGCTTCTTAGCTCATCAATTAGAAGAGGGCGCTCCGGTTAAAGTCTTCG
TCGAACACAATAACAACTTCAAATTGCCGAGTGATGATAATGCGCCGCTGATCATGGTTGGCCCAGGAACCGGAATTGCC
CCTTTCCGTAGCTTCATCCAAGAGCGCGAAAACCGTGGTGCAGCAGGAAAAAACTGGCTGTTGTTTGGTGACCGCACTTT
TACCCAAGATTTCCTCTACCAAGTCGAGTGGCAAAAGTACCTGAAGTCTGGTGTGTTAAATCGCTTAGATGTGGCTTTTA
GTCGCGACCAGCACGAAAAGGTGTATGTGCAGCATCGCCTTTTAGAACAAGCAGAGTTGGTATGGCAATGGTTACAAGAA
GGCGCTTACTTCTACGTATGTGGTGATGCATCACGCATGGCGAAGGATGTGCACCAAGCATTAATTACTGTTGTCGAGCA
ACAAGGTGGCTTGAATCGTGAGCAAGCTGAAGAGTATGTGAGTGAACTGCGTAAAGCAAAACGTTATCAAAGGGATGTCT
ACTAA

Upstream 100 bases:

>100_bases
ATTGAAAACCAGTAGCGAGATGGTTAAGTCAAAAAGTTATAAAACATTCACATCTATTCTTTCTTGTTATTAAAAAGAGT
GATTAATCTATCGTCATGCA

Downstream 100 bases:

>100_bases
TGAGCGCAAATCAAAACCCATCAGTACAAGAGGTGCTTGGCGAAGTGCTCGGCCCATGGTCGGATAATGAACGCCTAAAG
CGCGAGAGTCATTTTCTACG

Product: sulfite reductase (NADPH) flavoprotein alpha-component

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MNREVVTMSTGNTLPPALAALASPLNDAQLNQLQQTVTQLNAQQLAWVSGYFWGLSQSNALSVPHISAGQTASAASGKLT
IIFASQTGNAKGVAQALLKEAQAAGIQAQLFDASDYKGKDLAKETHVIFVASTNGEGEAPDNALALHEFLKSKKAPKLPN
LKYGVLGLGDSSYQFFCQTGKDFDQFLENLGAQRLVERLDADVDYQAAATEWRKQVLSILKDELTGAAAVTSVATFAVSQ
TAESHYSKEQPYTASLSTSQKITGRDSGKDVRHIEIDLADSGITYQPGDALGVWYENRPQLVNALLDSVGLSGHEEVQVD
GETLSLHSALTHHYEITAANPQLVAQFAELAQSEKLTSLAQDKEALREYATRTQVIDVLREEKVTLSAIQLLSLLRRLTP
RLYSIASSQSEVGEEVHLTVGVVEYEYEGEQRLGGASSFLAHQLEEGAPVKVFVEHNNNFKLPSDDNAPLIMVGPGTGIA
PFRSFIQERENRGAAGKNWLLFGDRTFTQDFLYQVEWQKYLKSGVLNRLDVAFSRDQHEKVYVQHRLLEQAELVWQWLQE
GAYFYVCGDASRMAKDVHQALITVVEQQGGLNREQAEEYVSELRKAKRYQRDVY

Sequences:

>Translated_614_residues
MNREVVTMSTGNTLPPALAALASPLNDAQLNQLQQTVTQLNAQQLAWVSGYFWGLSQSNALSVPHISAGQTASAASGKLT
IIFASQTGNAKGVAQALLKEAQAAGIQAQLFDASDYKGKDLAKETHVIFVASTNGEGEAPDNALALHEFLKSKKAPKLPN
LKYGVLGLGDSSYQFFCQTGKDFDQFLENLGAQRLVERLDADVDYQAAATEWRKQVLSILKDELTGAAAVTSVATFAVSQ
TAESHYSKEQPYTASLSTSQKITGRDSGKDVRHIEIDLADSGITYQPGDALGVWYENRPQLVNALLDSVGLSGHEEVQVD
GETLSLHSALTHHYEITAANPQLVAQFAELAQSEKLTSLAQDKEALREYATRTQVIDVLREEKVTLSAIQLLSLLRRLTP
RLYSIASSQSEVGEEVHLTVGVVEYEYEGEQRLGGASSFLAHQLEEGAPVKVFVEHNNNFKLPSDDNAPLIMVGPGTGIA
PFRSFIQERENRGAAGKNWLLFGDRTFTQDFLYQVEWQKYLKSGVLNRLDVAFSRDQHEKVYVQHRLLEQAELVWQWLQE
GAYFYVCGDASRMAKDVHQALITVVEQQGGLNREQAEEYVSELRKAKRYQRDVY
>Mature_614_residues
MNREVVTMSTGNTLPPALAALASPLNDAQLNQLQQTVTQLNAQQLAWVSGYFWGLSQSNALSVPHISAGQTASAASGKLT
IIFASQTGNAKGVAQALLKEAQAAGIQAQLFDASDYKGKDLAKETHVIFVASTNGEGEAPDNALALHEFLKSKKAPKLPN
LKYGVLGLGDSSYQFFCQTGKDFDQFLENLGAQRLVERLDADVDYQAAATEWRKQVLSILKDELTGAAAVTSVATFAVSQ
TAESHYSKEQPYTASLSTSQKITGRDSGKDVRHIEIDLADSGITYQPGDALGVWYENRPQLVNALLDSVGLSGHEEVQVD
GETLSLHSALTHHYEITAANPQLVAQFAELAQSEKLTSLAQDKEALREYATRTQVIDVLREEKVTLSAIQLLSLLRRLTP
RLYSIASSQSEVGEEVHLTVGVVEYEYEGEQRLGGASSFLAHQLEEGAPVKVFVEHNNNFKLPSDDNAPLIMVGPGTGIA
PFRSFIQERENRGAAGKNWLLFGDRTFTQDFLYQVEWQKYLKSGVLNRLDVAFSRDQHEKVYVQHRLLEQAELVWQWLQE
GAYFYVCGDASRMAKDVHQALITVVEQQGGLNREQAEEYVSELRKAKRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI7657393, Length=599, Percent_Identity=33.3889816360601, Blast_Score=258, Evalue=9e-69,
Organism=Homo sapiens, GI127139033, Length=645, Percent_Identity=31.1627906976744, Blast_Score=258, Evalue=1e-68,
Organism=Homo sapiens, GI221316709, Length=599, Percent_Identity=33.3889816360601, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI221316705, Length=608, Percent_Identity=32.8947368421053, Blast_Score=251, Evalue=2e-66,
Organism=Homo sapiens, GI24041029, Length=600, Percent_Identity=31.8333333333333, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI221316707, Length=528, Percent_Identity=33.7121212121212, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI10835173, Length=651, Percent_Identity=29.9539170506912, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI40254422, Length=657, Percent_Identity=29.2237442922374, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI169790958, Length=429, Percent_Identity=30.5361305361305, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI169790956, Length=429, Percent_Identity=30.5361305361305, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI1789123, Length=591, Percent_Identity=56.175972927242, Blast_Score=668, Evalue=0.0,
Organism=Escherichia coli, GI1789154, Length=125, Percent_Identity=35.2, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17554134, Length=593, Percent_Identity=30.0168634064081, Blast_Score=249, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI17566446, Length=595, Percent_Identity=28.2352941176471, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17531441, Length=395, Percent_Identity=25.3164556962025, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI32563863, Length=125, Percent_Identity=36, Blast_Score=70, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321832, Length=661, Percent_Identity=29.803328290469, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6325305, Length=631, Percent_Identity=25.1980982567353, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6321143, Length=392, Percent_Identity=29.0816326530612, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6322597, Length=162, Percent_Identity=32.7160493827161, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17137192, Length=608, Percent_Identity=30.4276315789474, Blast_Score=249, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24582192, Length=516, Percent_Identity=30.6201550387597, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24660907, Length=593, Percent_Identity=28.3305227655987, Blast_Score=195, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24660903, Length=593, Percent_Identity=28.3305227655987, Blast_Score=195, Evalue=1e-49,
Organism=Drosophila melanogaster, GI78706872, Length=524, Percent_Identity=28.8167938931298, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24583543, Length=524, Percent_Identity=28.8167938931298, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI78706876, Length=524, Percent_Identity=28.8167938931298, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24647438, Length=415, Percent_Identity=24.578313253012, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 67702; Mature: 67702

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREVVTMSTGNTLPPALAALASPLNDAQLNQLQQTVTQLNAQQLAWVSGYFWGLSQSNA
CCCEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LSVPHISAGQTASAASGKLTIIFASQTGNAKGVAQALLKEAQAAGIQAQLFDASDYKGKD
CCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCC
LAKETHVIFVASTNGEGEAPDNALALHEFLKSKKAPKLPNLKYGVLGLGDSSYQFFCQTG
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECCC
KDFDQFLENLGAQRLVERLDADVDYQAAATEWRKQVLSILKDELTGAAAVTSVATFAVSQ
CCHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TAESHYSKEQPYTASLSTSQKITGRDSGKDVRHIEIDLADSGITYQPGDALGVWYENRPQ
HHHHHCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEEECCCCEECCCCCEEEEECCCHH
LVNALLDSVGLSGHEEVQVDGETLSLHSALTHHYEITAANPQLVAQFAELAQSEKLTSLA
HHHHHHHHHCCCCCCEEEECCCEEHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHHHH
QDKEALREYATRTQVIDVLREEKVTLSAIQLLSLLRRLTPRLYSIASSQSEVGEEVHLTV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEEE
GVVEYEYEGEQRLGGASSFLAHQLEEGAPVKVFVEHNNNFKLPSDDNAPLIMVGPGTGIA
EEEEEEECCHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCHH
PFRSFIQERENRGAAGKNWLLFGDRTFTQDFLYQVEWQKYLKSGVLNRLDVAFSRDQHEK
HHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VYVQHRLLEQAELVWQWLQEGAYFYVCGDASRMAKDVHQALITVVEQQGGLNREQAEEYV
HHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
SELRKAKRYQRDVY
HHHHHHHHHHHCCC
>Mature Secondary Structure
MNREVVTMSTGNTLPPALAALASPLNDAQLNQLQQTVTQLNAQQLAWVSGYFWGLSQSNA
CCCEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LSVPHISAGQTASAASGKLTIIFASQTGNAKGVAQALLKEAQAAGIQAQLFDASDYKGKD
CCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCC
LAKETHVIFVASTNGEGEAPDNALALHEFLKSKKAPKLPNLKYGVLGLGDSSYQFFCQTG
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECCC
KDFDQFLENLGAQRLVERLDADVDYQAAATEWRKQVLSILKDELTGAAAVTSVATFAVSQ
CCHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TAESHYSKEQPYTASLSTSQKITGRDSGKDVRHIEIDLADSGITYQPGDALGVWYENRPQ
HHHHHCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEEECCCCEECCCCCEEEEECCCHH
LVNALLDSVGLSGHEEVQVDGETLSLHSALTHHYEITAANPQLVAQFAELAQSEKLTSLA
HHHHHHHHHCCCCCCEEEECCCEEHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHHHH
QDKEALREYATRTQVIDVLREEKVTLSAIQLLSLLRRLTPRLYSIASSQSEVGEEVHLTV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEEE
GVVEYEYEGEQRLGGASSFLAHQLEEGAPVKVFVEHNNNFKLPSDDNAPLIMVGPGTGIA
EEEEEEECCHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCHH
PFRSFIQERENRGAAGKNWLLFGDRTFTQDFLYQVEWQKYLKSGVLNRLDVAFSRDQHEK
HHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VYVQHRLLEQAELVWQWLQEGAYFYVCGDASRMAKDVHQALITVVEQQGGLNREQAEEYV
HHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
SELRKAKRYQRDVY
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA