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The map label for this gene is cysH

Identifier: 227080598

GI number: 227080598

Start: 385173

End: 385934

Strand: Direct

Name: cysH

Synonym: VCM66_0371

Alternate gene names: 227080598

Gene position: 385173-385934 (Clockwise)

Preceding gene: 227080597

Following gene: 227080599

Centisome position: 13.32

GC content: 45.41

Gene sequence:

>762_bases
ATGCCTAATCGTACCGTTCCAACACTAGAAGAGTTACTAACGTTAAATAAGGTGCAGCAAACGCTGCGCCTAACGGAAGT
CAATCAGCATCTGGAATCCCTCACTGCGCAAGAGCGCGTCGTCTGGGGTCTAGAAAATCTGCAGGGCAATCATGCACTGT
CATCGAGCTTTGGTATCCAAGCCGCAGTGATGCTTCATTTATTAACTTCAGTGAAGAGTGACATTCCTGTCGTTTTAACC
GATACCGGCTATCTGTTCCCAGAAACCTATCAGTTTATCGATGAGTTGACTGAGCGGCTCAATCTGAACTTAAAAGTTTA
CTCCGCGCCTGTATCAGCGGCTTGGCAAGAAGCTCGCTACGGAAAACTGTGGGAACAAGGTGTTGAAGGAATCGAACGCT
ACAACCAGATTAATAAAGTAGAACCGATGCGTAGAGCATTAGATGAACTCAACATTGGCACTTGGTTCTCGGGCTTACGA
CGTGAACAGTCACAATCACGAGCAAGCCTACCGATTCTATCGGTACAAAATGGCGTGTTTAAATTCCTGCCAGTGATCGA
TTGGACCAATAAAGAGGTGCACTATTATCTCAAGGATAACGATCTACCTTATCACCCACTTTGGGAACAAGGTTATCTTT
CGGTTGGTGATACACACACCACACAGAAATGGCAGCCGGGAATGAATGAAGAGCAAACTCGCTTCTTTGGTTTAAAGCGT
GAGTGTGGGTTACACGAAGATCATAACGATACACATCAATAA

Upstream 100 bases:

>100_bases
GGACGTTGGGCTAGCGAGCGTCTGGACGGTGAAGGATTTGGTGATTTCACTATTCGTGCAGGCATTATCGAAGAGGTAAT
CATCTCGAAGAGGGATTTCT

Downstream 100 bases:

>100_bases
ACGCGTTCATTTTCTATTTACACATAATAAAGAGAGGCTGCCGTGATGTAGCCTCTTGCTTTATCTCAGCCTACAAATGA
ATAACTCTGTGGATAAGTTG

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MPNRTVPTLEELLTLNKVQQTLRLTEVNQHLESLTAQERVVWGLENLQGNHALSSSFGIQAAVMLHLLTSVKSDIPVVLT
DTGYLFPETYQFIDELTERLNLNLKVYSAPVSAAWQEARYGKLWEQGVEGIERYNQINKVEPMRRALDELNIGTWFSGLR
REQSQSRASLPILSVQNGVFKFLPVIDWTNKEVHYYLKDNDLPYHPLWEQGYLSVGDTHTTQKWQPGMNEEQTRFFGLKR
ECGLHEDHNDTHQ

Sequences:

>Translated_253_residues
MPNRTVPTLEELLTLNKVQQTLRLTEVNQHLESLTAQERVVWGLENLQGNHALSSSFGIQAAVMLHLLTSVKSDIPVVLT
DTGYLFPETYQFIDELTERLNLNLKVYSAPVSAAWQEARYGKLWEQGVEGIERYNQINKVEPMRRALDELNIGTWFSGLR
REQSQSRASLPILSVQNGVFKFLPVIDWTNKEVHYYLKDNDLPYHPLWEQGYLSVGDTHTTQKWQPGMNEEQTRFFGLKR
ECGLHEDHNDTHQ
>Mature_252_residues
PNRTVPTLEELLTLNKVQQTLRLTEVNQHLESLTAQERVVWGLENLQGNHALSSSFGIQAAVMLHLLTSVKSDIPVVLTD
TGYLFPETYQFIDELTERLNLNLKVYSAPVSAAWQEARYGKLWEQGVEGIERYNQINKVEPMRRALDELNIGTWFSGLRR
EQSQSRASLPILSVQNGVFKFLPVIDWTNKEVHYYLKDNDLPYHPLWEQGYLSVGDTHTTQKWQPGMNEEQTRFFGLKRE
CGLHEDHNDTHQ

Specific function: Reduction of activated sulfate into sulfite

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily

Homologues:

Organism=Escherichia coli, GI1789121, Length=238, Percent_Identity=70.1680672268908, Blast_Score=356, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6325425, Length=226, Percent_Identity=33.6283185840708, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSH_VIBC3 (A5F3I6)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F3I6
- SMR:   A5F3I6
- STRING:   A5F3I6
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2797
- eggNOG:   COG0175
- HOGENOM:   HBG758022
- OMA:   QVNPSAK
- ProtClustDB:   PRK02090
- BioCyc:   VCHO345073:VC0395_A2797-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00063
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR011800
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00434
- TIGRFAMs:   TIGR02057

Pfam domain/function: PF01507 PAPS_reduct

EC number: =1.8.4.8

Molecular weight: Translated: 29249; Mature: 29118

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNRTVPTLEELLTLNKVQQTLRLTEVNQHLESLTAQERVVWGLENLQGNHALSSSFGIQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHH
AAVMLHLLTSVKSDIPVVLTDTGYLFPETYQFIDELTERLNLNLKVYSAPVSAAWQEARY
HHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
GKLWEQGVEGIERYNQINKVEPMRRALDELNIGTWFSGLRREQSQSRASLPILSVQNGVF
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCCHH
KFLPVIDWTNKEVHYYLKDNDLPYHPLWEQGYLSVGDTHTTQKWQPGMNEEQTRFFGLKR
HHHHCEECCCCEEEEEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHCCHH
ECGLHEDHNDTHQ
HCCCCCCCCCCCC
>Mature Secondary Structure 
PNRTVPTLEELLTLNKVQQTLRLTEVNQHLESLTAQERVVWGLENLQGNHALSSSFGIQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHH
AAVMLHLLTSVKSDIPVVLTDTGYLFPETYQFIDELTERLNLNLKVYSAPVSAAWQEARY
HHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
GKLWEQGVEGIERYNQINKVEPMRRALDELNIGTWFSGLRREQSQSRASLPILSVQNGVF
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCCHH
KFLPVIDWTNKEVHYYLKDNDLPYHPLWEQGYLSVGDTHTTQKWQPGMNEEQTRFFGLKR
HHHHCEECCCCEEEEEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHCCHH
ECGLHEDHNDTHQ
HCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA