Click here to switch to the map view.

The map label for this gene is hflK

Identifier: 227080561

GI number: 227080561

Start: 356507

End: 357694

Strand: Direct

Name: hflK

Synonym: VCM66_0333

Alternate gene names: 227080561

Gene position: 356507-357694 (Clockwise)

Preceding gene: 227080560

Following gene: 227080562

Centisome position: 12.33

GC content: 49.49

Gene sequence:

>1188_bases
ATGGCGTGGAATGAGCCTGGTAATAATAACGGCAACAATGGCCGCGATAATGACCCATGGGGGAACAACAATCGCGGTAA
CAAAGGTGGCCGAGATCAAGGACCGCCAGATTTAGACGAAGTGTTTAATAAACTGAGTCAAAAGCTGGGGGGCAAGTTTG
GTGGCAAAGGTGGTAAAGGCCCTTCGTTTTCCGGTGGCGGTGCGATTGGCTTTGGTGTCATTGCAGCTATCGCTGTTGCG
GTTTGGTTCTTTACTGGTTTTTATACCATCGGTGAAGCTGAGCGCGGCGTTGTGCTACGCCTTGGCAAATACGACCGCAT
TGTTGATCCGGGTCTGAACTGGCGTCCACGCTTTATTGATGAAGTGACACCAGTCAACGTGCAAGCGATTCGCTCTCTGC
GCGCCTCTGGCTTGATGCTGACCAAAGATGAAAACGTGGTAACGGTATCGATGGATGTGCAATATCGTATTGCCGATCCA
TACAAATACTTGTATCGCGTGACCAACGCGGATGACAGCTTGCGTCAAGCAACGGACTCTGCGCTTCGTGCCGTAGTCGG
TGACTCACTGATGGATAGCATCCTAACCAGTGGTCGTCAGCAAATTCGCCAAAGCACGCAGCAGACACTCAACCAAGTCA
TCGATAGCTATGATATGGGCTTGATGATTGTTGATGTGAACTTCCAGTCGGCTCGTCCACCTGAGCAAGTAAAAGATGCC
TTTGACGATGCGATTGCAGCCCGTGAGGATGAAGAGCGTTTCATTCGCGAAGCGGAAGCTTACAAGAACGAAATTTTGCC
TAAAGCCACAGGCCGTGCTGAGCGTCTGAAGAAAGAAGCGCAAGGTTACAACGAGCGCACCATCAATGAAGCGCTCGGTC
AGGTTGCTCAGTTTGAAAAGCTGTTGCCTGAGTATCAAGCTGCACCGAAAGTAACCCGTGACCGTCTCTATTTGGATGCG
ATGGAGCAGGTGTATAGCAATACTTCAAAAGTACTGATTGATTCTGAATCGAGCGGTAATCTGCTTTATCTGCCGATTGA
TAAGTTAGCGGGTCAAGACAATAAAACGGCGCAGCCGCGTCCAAATAAGTCTTCATCAGCCTACGATCAAATTGAGCTTG
AATCACAAACGACAGAAACGAATACCGACACTCAGTCGCGTAGCACCACACGTCAAGGGAGATACTAA

Upstream 100 bases:

>100_bases
GGCTAGACTAGAAAAACGAGAGCAAGCAGATTTATGTGACTTTATAGTTACTTAAAGGACTGCTAGAGTATAACGTCATA
TCAAATGATGGAGTTTTCTA

Downstream 100 bases:

>100_bases
GAATGCGTAAGTTATTAATCCCTAGTATCGTTCTGATCATCGCTGCTTTATTGATGTCGATGTTCGTGATCCCAGAGGGC
GAGCGCGGAATCGTGATCCG

Product: hflK protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAWNEPGNNNGNNGRDNDPWGNNNRGNKGGRDQGPPDLDEVFNKLSQKLGGKFGGKGGKGPSFSGGGAIGFGVIAAIAVA
VWFFTGFYTIGEAERGVVLRLGKYDRIVDPGLNWRPRFIDEVTPVNVQAIRSLRASGLMLTKDENVVTVSMDVQYRIADP
YKYLYRVTNADDSLRQATDSALRAVVGDSLMDSILTSGRQQIRQSTQQTLNQVIDSYDMGLMIVDVNFQSARPPEQVKDA
FDDAIAAREDEERFIREAEAYKNEILPKATGRAERLKKEAQGYNERTINEALGQVAQFEKLLPEYQAAPKVTRDRLYLDA
MEQVYSNTSKVLIDSESSGNLLYLPIDKLAGQDNKTAQPRPNKSSSAYDQIELESQTTETNTDTQSRSTTRQGRY

Sequences:

>Translated_395_residues
MAWNEPGNNNGNNGRDNDPWGNNNRGNKGGRDQGPPDLDEVFNKLSQKLGGKFGGKGGKGPSFSGGGAIGFGVIAAIAVA
VWFFTGFYTIGEAERGVVLRLGKYDRIVDPGLNWRPRFIDEVTPVNVQAIRSLRASGLMLTKDENVVTVSMDVQYRIADP
YKYLYRVTNADDSLRQATDSALRAVVGDSLMDSILTSGRQQIRQSTQQTLNQVIDSYDMGLMIVDVNFQSARPPEQVKDA
FDDAIAAREDEERFIREAEAYKNEILPKATGRAERLKKEAQGYNERTINEALGQVAQFEKLLPEYQAAPKVTRDRLYLDA
MEQVYSNTSKVLIDSESSGNLLYLPIDKLAGQDNKTAQPRPNKSSSAYDQIELESQTTETNTDTQSRSTTRQGRY
>Mature_394_residues
AWNEPGNNNGNNGRDNDPWGNNNRGNKGGRDQGPPDLDEVFNKLSQKLGGKFGGKGGKGPSFSGGGAIGFGVIAAIAVAV
WFFTGFYTIGEAERGVVLRLGKYDRIVDPGLNWRPRFIDEVTPVNVQAIRSLRASGLMLTKDENVVTVSMDVQYRIADPY
KYLYRVTNADDSLRQATDSALRAVVGDSLMDSILTSGRQQIRQSTQQTLNQVIDSYDMGLMIVDVNFQSARPPEQVKDAF
DDAIAAREDEERFIREAEAYKNEILPKATGRAERLKKEAQGYNERTINEALGQVAQFEKLLPEYQAAPKVTRDRLYLDAM
EQVYSNTSKVLIDSESSGNLLYLPIDKLAGQDNKTAQPRPNKSSSAYDQIELESQTTETNTDTQSRSTTRQGRY

Specific function: HflC and HflK could encode or regulate a protease

COG id: COG0330

COG function: function code O; Membrane protease subunits, stomatin/prohibitin homologs

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the band 7/mec-2 family. HflK subfamily

Homologues:

Organism=Escherichia coli, GI1790616, Length=381, Percent_Identity=56.9553805774278, Blast_Score=390, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HFLK_VIBCH (Q9KV09)

Other databases:

- EMBL:   AE003852
- PIR:   E82334
- RefSeq:   NP_230003.1
- ProteinModelPortal:   Q9KV09
- SMR:   Q9KV09
- GeneID:   2615062
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0349
- TIGR:   VC_0349
- HOGENOM:   HBG465357
- OMA:   WNEPGGN
- ProtClustDB:   CLSK873942
- InterPro:   IPR001107
- InterPro:   IPR010201
- InterPro:   IPR020980
- InterPro:   IPR001972
- PANTHER:   PTHR10264
- PRINTS:   PR00721
- SMART:   SM00244
- TIGRFAMs:   TIGR01933

Pfam domain/function: PF01145 Band_7; PF12221 HflK_N

EC number: NA

Molecular weight: Translated: 43815; Mature: 43684

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x24a5183c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWNEPGNNNGNNGRDNDPWGNNNRGNKGGRDQGPPDLDEVFNKLSQKLGGKFGGKGGKG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
PSFSGGGAIGFGVIAAIAVAVWFFTGFYTIGEAERGVVLRLGKYDRIVDPGLNWRPRFID
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHH
EVTPVNVQAIRSLRASGLMLTKDENVVTVSMDVQYRIADPYKYLYRVTNADDSLRQATDS
CCCCCCHHHHHHHHHCCEEEECCCCEEEEEEEEEEEECCHHHHHHHHCCCCHHHHHHHHH
ALRAVVGDSLMDSILTSGRQQIRQSTQQTLNQVIDSYDMGLMIVDVNFQSARPPEQVKDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHH
FDDAIAAREDEERFIREAEAYKNEILPKATGRAERLKKEAQGYNERTINEALGQVAQFEK
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LLPEYQAAPKVTRDRLYLDAMEQVYSNTSKVLIDSESSGNLLYLPIDKLAGQDNKTAQPR
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEHHHHCCCCCCCCCCC
PNKSSSAYDQIELESQTTETNTDTQSRSTTRQGRY
CCCCCCCHHHEEECCCCCCCCCCCHHHHCCCCCCH
>Mature Secondary Structure 
AWNEPGNNNGNNGRDNDPWGNNNRGNKGGRDQGPPDLDEVFNKLSQKLGGKFGGKGGKG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
PSFSGGGAIGFGVIAAIAVAVWFFTGFYTIGEAERGVVLRLGKYDRIVDPGLNWRPRFID
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHH
EVTPVNVQAIRSLRASGLMLTKDENVVTVSMDVQYRIADPYKYLYRVTNADDSLRQATDS
CCCCCCHHHHHHHHHCCEEEECCCCEEEEEEEEEEEECCHHHHHHHHCCCCHHHHHHHHH
ALRAVVGDSLMDSILTSGRQQIRQSTQQTLNQVIDSYDMGLMIVDVNFQSARPPEQVKDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHH
FDDAIAAREDEERFIREAEAYKNEILPKATGRAERLKKEAQGYNERTINEALGQVAQFEK
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LLPEYQAAPKVTRDRLYLDAMEQVYSNTSKVLIDSESSGNLLYLPIDKLAGQDNKTAQPR
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEHHHHCCCCCCCCCCC
PNKSSSAYDQIELESQTTETNTDTQSRSTTRQGRY
CCCCCCCHHHEEECCCCCCCCCCCHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301