Click here to switch to the map view.

The map label for this gene is tufB [H]

Identifier: 227080533

GI number: 227080533

Start: 320052

End: 321236

Strand: Direct

Name: tufB [H]

Synonym: VCM66_0305

Alternate gene names: 227080533

Gene position: 320052-321236 (Clockwise)

Preceding gene: 227080531

Following gene: 227080534

Centisome position: 11.06

GC content: 49.03

Gene sequence:

>1185_bases
ATGTCTAAAGAGAAATTTGAACGTACGAAACCGCACGTAAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACAAC
TCTAACTGCAGCTATCTGTACTGTACTTGCAAAAGTATACGGCGGTAAAGCTCGTGACTTCGCGTCAATCGACAACGCAC
CAGAAGAGCGTGAGCGTGGTATTACAATCAATACTTCTCACGTAGAGTACGATACTCCAAACCGTCACTACGCACACGTA
GACTGTCCAGGACACGCGGATTATGTTAAGAACATGATCACCGGTGCAGCACAGATGGACGGCGGTATCCTAGTTGTAGC
GGCAACTGACGGTCCAATGCCACAAACTCGTGAGCACATCCTGCTGGGTCGCCAAGTAGGTATTCCTTACATCATCGTAT
TCATGAACAAGTGTGACATGGTTGACGATGAAGAGCTTCTAGAGCTGGTAGAGATGGAAGTTCGTGAGCTGCTGTCTGAG
TACGATTTCCCAGGTGATGACCTGCCAGTAATCCAAGGTTCAGCACTAGGCGCGCTAAACGGCGAAGCACAGTGGGAAGC
GAAGATTGTTGAGCTAGCAGAAGCACTGGATACTTATATTCCAGAGCCAGAGCGTGCAGTAGACATGGCATTCCTGATGC
CAATCGAAGACGTATTCTCAATCCAAGGTCGTGGTACAGTAGTAACTGGCCGTATCGAGCGCGGCATCCTGAAAGTGGGT
GACGAAGTAGCGATCGTTGGTATCAAAGAGACAGTAAAAACGACCTGTACAGGTGTAGAGATGTTCCGTAAGCTGCTTGA
CGAAGGTCGTGCAGGTGAGAACGTAGGTGCACTACTACGTGGTACTAAGCGTGAAGAAGTAGAGCGTGGTCAAGTACTGG
CGAAGCCAGGTTCAATCACACCACACACTAAGTTCGAATCAGAAGTATACGTACTGTCAAAAGATGAAGGTGGCCGTCAT
ACTCCATTCTTCAAAGGTTACCGTCCACAGTTCTACTTCCGTACAACTGACGTAACAGGCAGCATCGAGCTACCAGAGGG
CGTAGAAATGGTAATGCCAGGCGACAACGTGAAGATGGTTGTAGACCTGATTGCACCAATCGCGATGGACGAAGGTCTAC
GCTTCGCTATCCGTGAAGGTGGCCGTACTGTAGGTGCTGGTGTTGTAGCTAAGATCATCGCTTAA

Upstream 100 bases:

>100_bases
TAAGCAAAGTTTTCCTTTTGATATATACTTTACTACCGATATTTTTTGGTTGCGTGGTCATCTCAAAAGCCACCTAAATC
CGTACCTAGAGGGACAACTC

Downstream 100 bases:

>100_bases
TTATTTGACTAAGCACTACTAAAAAGGGCATCATTTGATGCCCTTTTTCTGCGCTGCGCAATAATGTGTCGATTTCTTGA
TCAAACGCAGCCAAAATTTG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTVLAKVYGGKARDFASIDNAPEERERGITINTSHVEYDTPNRHYAHV
DCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLGRQVGIPYIIVFMNKCDMVDDEELLELVEMEVRELLSE
YDFPGDDLPVIQGSALGALNGEAQWEAKIVELAEALDTYIPEPERAVDMAFLMPIEDVFSIQGRGTVVTGRIERGILKVG
DEVAIVGIKETVKTTCTGVEMFRKLLDEGRAGENVGALLRGTKREEVERGQVLAKPGSITPHTKFESEVYVLSKDEGGRH
TPFFKGYRPQFYFRTTDVTGSIELPEGVEMVMPGDNVKMVVDLIAPIAMDEGLRFAIREGGRTVGAGVVAKIIA

Sequences:

>Translated_394_residues
MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTVLAKVYGGKARDFASIDNAPEERERGITINTSHVEYDTPNRHYAHV
DCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLGRQVGIPYIIVFMNKCDMVDDEELLELVEMEVRELLSE
YDFPGDDLPVIQGSALGALNGEAQWEAKIVELAEALDTYIPEPERAVDMAFLMPIEDVFSIQGRGTVVTGRIERGILKVG
DEVAIVGIKETVKTTCTGVEMFRKLLDEGRAGENVGALLRGTKREEVERGQVLAKPGSITPHTKFESEVYVLSKDEGGRH
TPFFKGYRPQFYFRTTDVTGSIELPEGVEMVMPGDNVKMVVDLIAPIAMDEGLRFAIREGGRTVGAGVVAKIIA
>Mature_393_residues
SKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTVLAKVYGGKARDFASIDNAPEERERGITINTSHVEYDTPNRHYAHVD
CPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLGRQVGIPYIIVFMNKCDMVDDEELLELVEMEVRELLSEY
DFPGDDLPVIQGSALGALNGEAQWEAKIVELAEALDTYIPEPERAVDMAFLMPIEDVFSIQGRGTVVTGRIERGILKVGD
EVAIVGIKETVKTTCTGVEMFRKLLDEGRAGENVGALLRGTKREEVERGQVLAKPGSITPHTKFESEVYVLSKDEGGRHT
PFFKGYRPQFYFRTTDVTGSIELPEGVEMVMPGDNVKMVVDLIAPIAMDEGLRFAIREGGRTVGAGVVAKIIA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=56.2025316455696, Blast_Score=461, Evalue=1e-130,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=29.5454545454545, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=29.5454545454545, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI4503475, Length=446, Percent_Identity=28.0269058295964, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=28.3482142857143, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI54607086, Length=343, Percent_Identity=27.9883381924198, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI194018522, Length=333, Percent_Identity=28.2282282282282, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI194018520, Length=333, Percent_Identity=28.2282282282282, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI194097354, Length=333, Percent_Identity=28.2282282282282, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI46094014, Length=322, Percent_Identity=29.8136645962733, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4503507, Length=277, Percent_Identity=27.4368231046931, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI157426893, Length=265, Percent_Identity=26.7924528301887, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI53729339, Length=232, Percent_Identity=34.051724137931, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI53729337, Length=232, Percent_Identity=34.051724137931, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=34.0740740740741, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=87.3096446700508, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=87.5318066157761, Blast_Score=686, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=377, Percent_Identity=29.4429708222812, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789108, Length=330, Percent_Identity=27.8787878787879, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI48994988, Length=237, Percent_Identity=30.379746835443, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788922, Length=327, Percent_Identity=25.3822629969419, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=51.6290726817043, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25141371, Length=385, Percent_Identity=45.4545454545455, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=453, Percent_Identity=29.5805739514349, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17569207, Length=453, Percent_Identity=29.5805739514349, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=27.8801843317972, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI115532067, Length=444, Percent_Identity=28.1531531531532, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=441, Percent_Identity=28.1179138321995, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=451, Percent_Identity=25.0554323725055, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17506081, Length=298, Percent_Identity=28.8590604026846, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=34.8993288590604, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17509919, Length=279, Percent_Identity=26.1648745519713, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=230, Percent_Identity=27.8260869565217, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=62.7848101265823, Blast_Score=507, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6325337, Length=349, Percent_Identity=32.378223495702, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=349, Percent_Identity=32.378223495702, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320377, Length=337, Percent_Identity=29.3768545994065, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322937, Length=453, Percent_Identity=23.6203090507726, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=272, Percent_Identity=27.2058823529412, Blast_Score=83, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=252, Percent_Identity=27.7777777777778, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=31.1111111111111, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=55.470737913486, Blast_Score=460, Evalue=1e-130,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=55.470737913486, Blast_Score=460, Evalue=1e-130,
Organism=Drosophila melanogaster, GI19921738, Length=387, Percent_Identity=50.1291989664083, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI45553807, Length=453, Percent_Identity=28.0353200883002, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI45553816, Length=453, Percent_Identity=28.0353200883002, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24651721, Length=453, Percent_Identity=28.0353200883002, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17864154, Length=453, Percent_Identity=28.0353200883002, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24652838, Length=455, Percent_Identity=28.3516483516484, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI17137572, Length=455, Percent_Identity=28.3516483516484, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI19922690, Length=302, Percent_Identity=29.8013245033113, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI45550900, Length=438, Percent_Identity=27.3972602739726, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI17137380, Length=341, Percent_Identity=28.7390029325513, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24647046, Length=278, Percent_Identity=27.3381294964029, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24647048, Length=278, Percent_Identity=27.3381294964029, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28572034, Length=175, Percent_Identity=34.8571428571429, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43127; Mature: 42996

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTVLAKVYGGKARDFASIDNAPEERERG
CCHHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCCCHHHHCC
ITINTSHVEYDTPNRHYAHVDCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHI
EEEECCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHH
LLGRQVGIPYIIVFMNKCDMVDDEELLELVEMEVRELLSEYDFPGDDLPVIQGSALGALN
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
GEAQWEAKIVELAEALDTYIPEPERAVDMAFLMPIEDVFSIQGRGTVVTGRIERGILKVG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCHHHHHCCCCCCEEEECHHHCCHHHCC
DEVAIVGIKETVKTTCTGVEMFRKLLDEGRAGENVGALLRGTKREEVERGQVLAKPGSIT
CCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHCCCEEECCCCCC
PHTKFESEVYVLSKDEGGRHTPFFKGYRPQFYFRTTDVTGSIELPEGVEMVMPGDNVKMV
CCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCHHHH
VDLIAPIAMDEGLRFAIREGGRTVGAGVVAKIIA
HHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
SKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTVLAKVYGGKARDFASIDNAPEERERG
CHHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCCCHHHHCC
ITINTSHVEYDTPNRHYAHVDCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHI
EEEECCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHH
LLGRQVGIPYIIVFMNKCDMVDDEELLELVEMEVRELLSEYDFPGDDLPVIQGSALGALN
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
GEAQWEAKIVELAEALDTYIPEPERAVDMAFLMPIEDVFSIQGRGTVVTGRIERGILKVG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCHHHHHCCCCCCEEEECHHHCCHHHCC
DEVAIVGIKETVKTTCTGVEMFRKLLDEGRAGENVGALLRGTKREEVERGQVLAKPGSIT
CCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHCCCEEECCCCCC
PHTKFESEVYVLSKDEGGRHTPFFKGYRPQFYFRTTDVTGSIELPEGVEMVMPGDNVKMV
CCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCHHHH
VDLIAPIAMDEGLRFAIREGGRTVGAGVVAKIIA
HHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA