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The map label for this gene is birA [H]

Identifier: 227080531

GI number: 227080531

Start: 317401

End: 318363

Strand: Direct

Name: birA [H]

Synonym: VCM66_0303

Alternate gene names: 227080531

Gene position: 317401-318363 (Clockwise)

Preceding gene: 227080530

Following gene: 227080533

Centisome position: 10.97

GC content: 48.81

Gene sequence:

>963_bases
ATGAAAGAGCACAGCGCTAAACTAGCCATACTCAAACAATTAGCCGATGGCGATTTTCACTCTGGTGAAGTGTTGGGTGC
TCAGCTGGGGATCTCGCGAGCAGCCATCAGTAAGCATATTCAGGGCATCCGCGACTGGGGTGTGGATGTGTTTCGTGTAC
AGGGTAAAGGGTATCAACTTGCTCAAGCGATGACGTTGCTCGATCAATCGGTAATCCAATCGCAAGTGAATAATCCTGTC
GAGCTGCATCCGATTATTGGCTCGACCAATCAATATCTGCTCGATCATGTCGAGACGCTGGTATCGGGAACGGTCTGTTT
AGCAGAATATCAAGCCAGTGGTCGTGGTCGGCGTGGTCGGCATTGGGTTTCTCCATTTGGCGCTAATCTTTATCTCTCGA
TCTATTGGCGTTTAGATGCCGGTATGGCAGCCGCGATGGGGCTGAGTTTAGTCGTGGGTGTGGCCATTGTTGAAGCGCTG
GAAGCCATGGGAGTGGATGGCGTTAAACTAAAATGGCCAAACGATCTCTATTATCAAGATAAAAAGTTGGCGGGCATTTT
AGTCGAAATGTCTGGTCAAGCTGGTGCGGCAGCACATTTAGTGATCGGCATGGGGATCAACCTTGCTATGCGCGATAACG
AGGGCAATATCGATCAGCCTTGGATAAGTCTTGCAGAAGTGACTGGCCAAAGCCGGATTGATCGTAATGCTTTAGCGATT
AATCTGATTGCTGCGCTAGATCGCACCTTACGCCAGTATGAAATTTCAGGTATGCAAAACTTTGTTGAGCGTTGGAATCG
TTGGGATAACTTCATCGGTCGTCCAGTTAAACTGCTGATGGGGGCGAATGAGGTGCGCGGTATTGAGCGCGGGATTGATG
AACACGGCGGCGTATTATTAGAAACCGAAGAGGGGTTGAAAAGTTTTATTGGCGGTGAGATATCCCTACGTAAAAACGAC
TAG

Upstream 100 bases:

>100_bases
AGCGCGTGTTCAACTGCTATGGCATTGAACTTGAACATGAAGTGCGATTTATTGGCGAAAGTGAAGAAACCAATTTAAAG
CAATGGATGAGTGAACAAGC

Downstream 100 bases:

>100_bases
AGCAGAATTAAAGCCAAATAAAGAGGCGTCAGCCTCTTTTTCTTGAGCACTATTTACGGAGCAGGACTTCTTCTACCGTA
TGGTTAAGCCCTTTGCGTAA

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKEHSAKLAILKQLADGDFHSGEVLGAQLGISRAAISKHIQGIRDWGVDVFRVQGKGYQLAQAMTLLDQSVIQSQVNNPV
ELHPIIGSTNQYLLDHVETLVSGTVCLAEYQASGRGRRGRHWVSPFGANLYLSIYWRLDAGMAAAMGLSLVVGVAIVEAL
EAMGVDGVKLKWPNDLYYQDKKLAGILVEMSGQAGAAAHLVIGMGINLAMRDNEGNIDQPWISLAEVTGQSRIDRNALAI
NLIAALDRTLRQYEISGMQNFVERWNRWDNFIGRPVKLLMGANEVRGIERGIDEHGGVLLETEEGLKSFIGGEISLRKND

Sequences:

>Translated_320_residues
MKEHSAKLAILKQLADGDFHSGEVLGAQLGISRAAISKHIQGIRDWGVDVFRVQGKGYQLAQAMTLLDQSVIQSQVNNPV
ELHPIIGSTNQYLLDHVETLVSGTVCLAEYQASGRGRRGRHWVSPFGANLYLSIYWRLDAGMAAAMGLSLVVGVAIVEAL
EAMGVDGVKLKWPNDLYYQDKKLAGILVEMSGQAGAAAHLVIGMGINLAMRDNEGNIDQPWISLAEVTGQSRIDRNALAI
NLIAALDRTLRQYEISGMQNFVERWNRWDNFIGRPVKLLMGANEVRGIERGIDEHGGVLLETEEGLKSFIGGEISLRKND
>Mature_320_residues
MKEHSAKLAILKQLADGDFHSGEVLGAQLGISRAAISKHIQGIRDWGVDVFRVQGKGYQLAQAMTLLDQSVIQSQVNNPV
ELHPIIGSTNQYLLDHVETLVSGTVCLAEYQASGRGRRGRHWVSPFGANLYLSIYWRLDAGMAAAMGLSLVVGVAIVEAL
EAMGVDGVKLKWPNDLYYQDKKLAGILVEMSGQAGAAAHLVIGMGINLAMRDNEGNIDQPWISLAEVTGQSRIDRNALAI
NLIAALDRTLRQYEISGMQNFVERWNRWDNFIGRPVKLLMGANEVRGIERGIDEHGGVLLETEEGLKSFIGGEISLRKND

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=319, Percent_Identity=54.858934169279, Blast_Score=347, Evalue=4e-97,
Organism=Drosophila melanogaster, GI24644246, Length=230, Percent_Identity=28.2608695652174, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 35041; Mature: 35041

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEHSAKLAILKQLADGDFHSGEVLGAQLGISRAAISKHIQGIRDWGVDVFRVQGKGYQL
CCCCHHHHHHHHHHHCCCCCCCCEEHHHHCHHHHHHHHHHHHHHHCCCEEEEECCCCHHH
AQAMTLLDQSVIQSQVNNPVELHPIIGSTNQYLLDHVETLVSGTVCLAEYQASGRGRRGR
HHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHEEEHHHCCCCCCCC
HWVSPFGANLYLSIYWRLDAGMAAAMGLSLVVGVAIVEALEAMGVDGVKLKWPNDLYYQD
CCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
KKLAGILVEMSGQAGAAAHLVIGMGINLAMRDNEGNIDQPWISLAEVTGQSRIDRNALAI
CHHEEEEEEECCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHCCHHCCCHHHHH
NLIAALDRTLRQYEISGMQNFVERWNRWDNFIGRPVKLLMGANEVRGIERGIDEHGGVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHCHHHHCCEEE
ETEEGLKSFIGGEISLRKND
ECHHHHHHHHCCEEEEECCC
>Mature Secondary Structure
MKEHSAKLAILKQLADGDFHSGEVLGAQLGISRAAISKHIQGIRDWGVDVFRVQGKGYQL
CCCCHHHHHHHHHHHCCCCCCCCEEHHHHCHHHHHHHHHHHHHHHCCCEEEEECCCCHHH
AQAMTLLDQSVIQSQVNNPVELHPIIGSTNQYLLDHVETLVSGTVCLAEYQASGRGRRGR
HHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHEEEHHHCCCCCCCC
HWVSPFGANLYLSIYWRLDAGMAAAMGLSLVVGVAIVEALEAMGVDGVKLKWPNDLYYQD
CCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
KKLAGILVEMSGQAGAAAHLVIGMGINLAMRDNEGNIDQPWISLAEVTGQSRIDRNALAI
CHHEEEEEEECCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHCCHHCCCHHHHH
NLIAALDRTLRQYEISGMQNFVERWNRWDNFIGRPVKLLMGANEVRGIERGIDEHGGVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHCHHHHCCEEE
ETEEGLKSFIGGEISLRKND
ECHHHHHHHHCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]