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The map label for this gene is rhtC [H]

Identifier: 227080413

GI number: 227080413

Start: 183114

End: 183782

Strand: Reverse

Name: rhtC [H]

Synonym: VCM66_0179

Alternate gene names: 227080413

Gene position: 183782-183114 (Counterclockwise)

Preceding gene: 227080414

Following gene: 227080409

Centisome position: 6.35

GC content: 50.97

Gene sequence:

>669_bases
ATGAACGAAATGTCCATTTTAGCCACCTTAGCCGGTGTGCATTTTATTGCCTTATTAAGCCCGGGTCCTGATGTCGCCTT
AGTGGTGCAAAACGCCACACAGCACGGGCGTAAAACTGGCGTGATGATCGCGTTAGGTTTGTCGTGCGGCATTTTAGTCC
ATTTGATTTTGAGCCTGACCGGCATTAGCTACTTAGTCAAACAGCAGCCAATGCTGTTTAACCTGTTACAACTGGCTGGC
GGCAGCTACTTGCTTTATCTGGGAGTCGGCGCTTTACAATCAGTGGTGGCGAAAAAAAACGCCAGCACACCGACGCACAG
CCCTGCGCTCTCCATTCTCGGCAACCGCCGTCAAGCCTTCACAAAAGGCATGATGACCAATCTCTTGAATCCCAAAGCTC
TCGTGTTTTTTGTCAGCCTGTTATCTAGCCTGATCCCAGCCAGCATGTCTGTGTCTGGCAAGGTGAGTGCCGCCCTGATT
TTGGTTGGCTTATCCCTAACTTGGTTCTCCTGTTTAGCTTGGCTGCTCACCACGTCTGCCATGCAACAACGTATGCAGCG
CATTACGCGATCCGTCGATAGCATATGCGCTGCAGTGTTTATCTTGGCCGGTGGCGTGATTTTATGGCAAGCAAGCCGCG
CTATAGCACAAACCTTCGGATGGCTATGA

Upstream 100 bases:

>100_bases
CTTCTTTGATCACTCGCTTTTCTTAACCACGCTGTCAAAAATTTACAAGCTCCTCGCGCTCACCGCGCGGCATACTCCGT
GAATAATGTGAGGAGAAAGC

Downstream 100 bases:

>100_bases
TCCGATTTTGGCCGATCATGCTGTGCGAGATCTCTTACAAAGCGGTAGGACAAATTCGCTTTCTGCGATAAAAAACACTG
ACAAAAACAACTTAAATAAT

Product: Threonine efflux protein

Products: Proton [Cytoplasm]; L-threonine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MNEMSILATLAGVHFIALLSPGPDVALVVQNATQHGRKTGVMIALGLSCGILVHLILSLTGISYLVKQQPMLFNLLQLAG
GSYLLYLGVGALQSVVAKKNASTPTHSPALSILGNRRQAFTKGMMTNLLNPKALVFFVSLLSSLIPASMSVSGKVSAALI
LVGLSLTWFSCLAWLLTTSAMQQRMQRITRSVDSICAAVFILAGGVILWQASRAIAQTFGWL

Sequences:

>Translated_222_residues
MNEMSILATLAGVHFIALLSPGPDVALVVQNATQHGRKTGVMIALGLSCGILVHLILSLTGISYLVKQQPMLFNLLQLAG
GSYLLYLGVGALQSVVAKKNASTPTHSPALSILGNRRQAFTKGMMTNLLNPKALVFFVSLLSSLIPASMSVSGKVSAALI
LVGLSLTWFSCLAWLLTTSAMQQRMQRITRSVDSICAAVFILAGGVILWQASRAIAQTFGWL
>Mature_222_residues
MNEMSILATLAGVHFIALLSPGPDVALVVQNATQHGRKTGVMIALGLSCGILVHLILSLTGISYLVKQQPMLFNLLQLAG
GSYLLYLGVGALQSVVAKKNASTPTHSPALSILGNRRQAFTKGMMTNLLNPKALVFFVSLLSSLIPASMSVSGKVSAALI
LVGLSLTWFSCLAWLLTTSAMQQRMQRITRSVDSICAAVFILAGGVILWQASRAIAQTFGWL

Specific function: Conducts the efflux of threonine [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994978, Length=192, Percent_Identity=31.7708333333333, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1788099, Length=186, Percent_Identity=29.5698924731183, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1786522, Length=207, Percent_Identity=27.0531400966184, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI48994979, Length=127, Percent_Identity=28.3464566929134, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23554; Mature: 23554

Theoretical pI: Translated: 10.97; Mature: 10.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEMSILATLAGVHFIALLSPGPDVALVVQNATQHGRKTGVMIALGLSCGILVHLILSLT
CCHHHHHHHHHHHHHHHEECCCCCEEEEEECCHHCCCCCCCEEEEHHHHHHHHHHHHHHH
GISYLVKQQPMLFNLLQLAGGSYLLYLGVGALQSVVAKKNASTPTHSPALSILGNRRQAF
HHHHHHHCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHH
TKGMMTNLLNPKALVFFVSLLSSLIPASMSVSGKVSAALILVGLSLTWFSCLAWLLTTSA
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQQRMQRITRSVDSICAAVFILAGGVILWQASRAIAQTFGWL
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNEMSILATLAGVHFIALLSPGPDVALVVQNATQHGRKTGVMIALGLSCGILVHLILSLT
CCHHHHHHHHHHHHHHHEECCCCCEEEEEECCHHCCCCCCCEEEEHHHHHHHHHHHHHHH
GISYLVKQQPMLFNLLQLAGGSYLLYLGVGALQSVVAKKNASTPTHSPALSILGNRRQAF
HHHHHHHCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHH
TKGMMTNLLNPKALVFFVSLLSSLIPASMSVSGKVSAALILVGLSLTWFSCLAWLLTTSA
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQQRMQRITRSVDSICAAVFILAGGVILWQASRAIAQTFGWL
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-threonine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + L-threonine [Cytoplasm] = Proton [Cytoplasm] + L-threonine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]