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The map label for this gene is hemC [H]

Identifier: 227080356

GI number: 227080356

Start: 114726

End: 115679

Strand: Reverse

Name: hemC [H]

Synonym: VCM66_0120

Alternate gene names: 227080356

Gene position: 115679-114726 (Counterclockwise)

Preceding gene: 227080357

Following gene: 227080355

Centisome position: 4.0

GC content: 54.19

Gene sequence:

>954_bases
ATGGATAGGAACATCATAATGACCGAGACGCCGATTCGCATTGCAACCCGCCAGAGTCCACTGGCTTTGTGGCAAGCCAA
CTATGTTAAAGACGCCTTAATGGCAGCCCACCCCGGACTGCAGGTGGAGTTGGTGACTATGGTCACGCGCGGCGATGTGA
TTCTGGATACTCCACTGGCTAAAGTGGGCGGTAAAGGCTTATTCGTGAAAGAGCTGGAAATTGCCATGCTCGAAGGGCGC
GCCGATTTAGCCGTGCATTCAATGAAAGATGTTCCGGTCGATTTCCCTGACGGTCTGGGCTTAGTCACCATCTGCGAACG
GGAAGATCCGCGTGATGCGTTTGTCTCAAATACCTACGCCAAGATTGAAGATCTGCCCTCGGGCGCTATCGTCGGTACCT
GCAGTTTACGCCGTCAATGCCAACTCAAAGCGGCACGGCCAGATTTAGTGATTAAAGAGCTGCGCGGCAACGTGGGGACT
CGCCTAAGTAAGCTTGATGCGGGCGAATACGATGCCATCATTTTAGCGGCAGCAGGCCTAAAACGCCTTGAGCTCGAAAG
CCGTATTCGTAGCTTTATTGAACCGGAACAGTCCCTACCTGCAGTTGGCCAAGGTGCCGTTGGTATTGAATGTCGCGTGA
ATGATCAACGCGTGCGCGCCCTACTCGCACCTCTTAACCATGCTGATACCGCCGATCGAGTGCGCTGCGAACGCGCGATG
AATCTCACGTTACAAGGTGGCTGTCAGGTGCCGATTGGTAGCTATGCACTGCTGGAGGGTGACACTATTTGGCTACGCGC
TCTTGTGGGTGAACCCGATGGTTCGCAAATCGTGCGCGGCGAAATTCGCGGACCTCGCACACAAGCTGAGCAACTTGGCA
TCACGCTGGCTGAGCAACTGCTTAGCCAAGGAGCCAAAGAGATCTTGGAGCGTCTCTACTGTGACCACGAATAA

Upstream 100 bases:

>100_bases
AAGCGGGAAAACGACATTCTTCTGCGCAAAATCGTTCCGACTCGAACTAGTTGTAAGCATTTTAAGCCCCAAGTGATAGG
ATTAGGCGATTTTAAAACAG

Downstream 100 bases:

>100_bases
TGGTAAGCCTATGTCCGCACGCATAGACTGATTGCTTATCTCGCATTGATTGAGAGTGTTATGACAGTACTGGTCACTCG
TCCTGATGAACAAGGCGCAG

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MDRNIIMTETPIRIATRQSPLALWQANYVKDALMAAHPGLQVELVTMVTRGDVILDTPLAKVGGKGLFVKELEIAMLEGR
ADLAVHSMKDVPVDFPDGLGLVTICEREDPRDAFVSNTYAKIEDLPSGAIVGTCSLRRQCQLKAARPDLVIKELRGNVGT
RLSKLDAGEYDAIILAAAGLKRLELESRIRSFIEPEQSLPAVGQGAVGIECRVNDQRVRALLAPLNHADTADRVRCERAM
NLTLQGGCQVPIGSYALLEGDTIWLRALVGEPDGSQIVRGEIRGPRTQAEQLGITLAEQLLSQGAKEILERLYCDHE

Sequences:

>Translated_317_residues
MDRNIIMTETPIRIATRQSPLALWQANYVKDALMAAHPGLQVELVTMVTRGDVILDTPLAKVGGKGLFVKELEIAMLEGR
ADLAVHSMKDVPVDFPDGLGLVTICEREDPRDAFVSNTYAKIEDLPSGAIVGTCSLRRQCQLKAARPDLVIKELRGNVGT
RLSKLDAGEYDAIILAAAGLKRLELESRIRSFIEPEQSLPAVGQGAVGIECRVNDQRVRALLAPLNHADTADRVRCERAM
NLTLQGGCQVPIGSYALLEGDTIWLRALVGEPDGSQIVRGEIRGPRTQAEQLGITLAEQLLSQGAKEILERLYCDHE
>Mature_317_residues
MDRNIIMTETPIRIATRQSPLALWQANYVKDALMAAHPGLQVELVTMVTRGDVILDTPLAKVGGKGLFVKELEIAMLEGR
ADLAVHSMKDVPVDFPDGLGLVTICEREDPRDAFVSNTYAKIEDLPSGAIVGTCSLRRQCQLKAARPDLVIKELRGNVGT
RLSKLDAGEYDAIILAAAGLKRLELESRIRSFIEPEQSLPAVGQGAVGIECRVNDQRVRALLAPLNHADTADRVRCERAM
NLTLQGGCQVPIGSYALLEGDTIWLRALVGEPDGSQIVRGEIRGPRTQAEQLGITLAEQLLSQGAKEILERLYCDHE

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps [H]

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family [H]

Homologues:

Organism=Homo sapiens, GI66933009, Length=332, Percent_Identity=41.566265060241, Blast_Score=231, Evalue=6e-61,
Organism=Homo sapiens, GI20149500, Length=332, Percent_Identity=41.566265060241, Blast_Score=231, Evalue=6e-61,
Organism=Escherichia coli, GI48994974, Length=307, Percent_Identity=75.8957654723127, Blast_Score=466, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6319996, Length=319, Percent_Identity=38.2445141065831, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI20130425, Length=260, Percent_Identity=42.6923076923077, Blast_Score=200, Evalue=8e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418 [H]

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC [H]

EC number: =2.5.1.61 [H]

Molecular weight: Translated: 34592; Mature: 34592

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRNIIMTETPIRIATRQSPLALWQANYVKDALMAAHPGLQVELVTMVTRGDVILDTPLA
CCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEEECCHH
KVGGKGLFVKELEIAMLEGRADLAVHSMKDVPVDFPDGLGLVTICEREDPRDAFVSNTYA
HHCCCCEEEEEEEEEEECCCCHHEEEHHCCCCCCCCCCCCEEEEECCCCCCHHHHHCHHH
KIEDLPSGAIVGTCSLRRQCQLKAARPDLVIKELRGNVGTRLSKLDAGEYDAIILAAAGL
HHHCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHCCCCCCCEEEEEECCC
KRLELESRIRSFIEPEQSLPAVGQGAVGIECRVNDQRVRALLAPLNHADTADRVRCERAM
HHHHHHHHHHHHHCCHHCCCCCCCCCEEEEEEECHHHHHHHHHCCCCCCCHHHHHHHHHH
NLTLQGGCQVPIGSYALLEGDTIWLRALVGEPDGSQIVRGEIRGPRTQAEQLGITLAEQL
CEEECCCCCCCCCCEEEECCCEEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHH
LSQGAKEILERLYCDHE
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDRNIIMTETPIRIATRQSPLALWQANYVKDALMAAHPGLQVELVTMVTRGDVILDTPLA
CCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEEECCHH
KVGGKGLFVKELEIAMLEGRADLAVHSMKDVPVDFPDGLGLVTICEREDPRDAFVSNTYA
HHCCCCEEEEEEEEEEECCCCHHEEEHHCCCCCCCCCCCCEEEEECCCCCCHHHHHCHHH
KIEDLPSGAIVGTCSLRRQCQLKAARPDLVIKELRGNVGTRLSKLDAGEYDAIILAAAGL
HHHCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHCCCCCCCEEEEEECCC
KRLELESRIRSFIEPEQSLPAVGQGAVGIECRVNDQRVRALLAPLNHADTADRVRCERAM
HHHHHHHHHHHHHCCHHCCCCCCCCCEEEEEEECHHHHHHHHHCCCCCCCHHHHHHHHHH
NLTLQGGCQVPIGSYALLEGDTIWLRALVGEPDGSQIVRGEIRGPRTQAEQLGITLAEQL
CEEECCCCCCCCCCEEEECCCEEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHH
LSQGAKEILERLYCDHE
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]