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The map label for this gene is hemD [H]

Identifier: 227080355

GI number: 227080355

Start: 113898

End: 114680

Strand: Reverse

Name: hemD [H]

Synonym: VCM66_0119

Alternate gene names: 227080355

Gene position: 114680-113898 (Counterclockwise)

Preceding gene: 227080356

Following gene: 227080354

Centisome position: 3.96

GC content: 50.32

Gene sequence:

>783_bases
TTGATTGAGAGTGTTATGACAGTACTGGTCACTCGTCCTGATGAACAAGGCGCAGAGCTGTGCCAGCTGCTGCACCAGCA
GGGCATCCCCGCCCTGCACCATCCGCTACTCGCCATCTCTGCCAGCCCTCAACTTGCTGCGCTGGCAGAAGATTTATCTT
CATTCGATATCATCATTGCCGTCAGTCAACATGCCGTCATCTTCAGTGACCAATTTCTGCAACATCGCCGAATTTGTTGG
CCAAATAACGCTATTTACCTTGCCGTTGGTCAAAAAACCGCGCATGTTTTCAGCAAAGCCTGCCAACAATCAGTAGACTA
TCCAGAAATAAGCGATAGTGAACATCTGCTGGCACTCAGTATGCTGCGTGATGTCGCAGGTAAGAAGGTATTGATCTTAC
GTGGTAATGGTGGTCGTGAGCTGATTTATTCCACCTTAGTGGAAAGAGGAGCGACTGTGCGCTATCAAGAAGCGTATTGT
CGGCACGAGCGGCCATTTGCCGCCGCCGAGTGTGTCCAAAAATGGCAGTTGGCGAAGGTCGACTCACTGGTGATCACCAG
CAGTGAGCAGTTGGCTTTTTTTATCCGTCAGTTCGAGACATCACACTTGCCATGGGTGCAGCAGTTAACTTTGCTCGTAC
CCAGCCAACGGATTGCCACTCTCGCCCACCAACTAGGATTTCATCGCATCGTGGTCACGCTAAGTGCTGCTAATCGCGAT
TTAGTCGCGGCATTGATGCCCGAAAAACCAACAGGAACTCTGAGCAATGACCAACAACAATAA

Upstream 100 bases:

>100_bases
GCTTAGCCAAGGAGCCAAAGAGATCTTGGAGCGTCTCTACTGTGACCACGAATAATGGTAAGCCTATGTCCGCACGCATA
GACTGATTGCTTATCTCGCA

Downstream 100 bases:

>100_bases
ACCGCAACAAGAACCTGAAATCACAGTAAACCCAAGCTCGGCAGCGGTGACTCCCCCGACCGCGCCCGACAAACAAGATA
GCCCAGCGCCAAACCAACCG

Product: uroporphyrinogen-III synthase

Products: NA

Alternate protein names: UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MIESVMTVLVTRPDEQGAELCQLLHQQGIPALHHPLLAISASPQLAALAEDLSSFDIIIAVSQHAVIFSDQFLQHRRICW
PNNAIYLAVGQKTAHVFSKACQQSVDYPEISDSEHLLALSMLRDVAGKKVLILRGNGGRELIYSTLVERGATVRYQEAYC
RHERPFAAAECVQKWQLAKVDSLVITSSEQLAFFIRQFETSHLPWVQQLTLLVPSQRIATLAHQLGFHRIVVTLSAANRD
LVAALMPEKPTGTLSNDQQQ

Sequences:

>Translated_260_residues
MIESVMTVLVTRPDEQGAELCQLLHQQGIPALHHPLLAISASPQLAALAEDLSSFDIIIAVSQHAVIFSDQFLQHRRICW
PNNAIYLAVGQKTAHVFSKACQQSVDYPEISDSEHLLALSMLRDVAGKKVLILRGNGGRELIYSTLVERGATVRYQEAYC
RHERPFAAAECVQKWQLAKVDSLVITSSEQLAFFIRQFETSHLPWVQQLTLLVPSQRIATLAHQLGFHRIVVTLSAANRD
LVAALMPEKPTGTLSNDQQQ
>Mature_260_residues
MIESVMTVLVTRPDEQGAELCQLLHQQGIPALHHPLLAISASPQLAALAEDLSSFDIIIAVSQHAVIFSDQFLQHRRICW
PNNAIYLAVGQKTAHVFSKACQQSVDYPEISDSEHLLALSMLRDVAGKKVLILRGNGGRELIYSTLVERGATVRYQEAYC
RHERPFAAAECVQKWQLAKVDSLVITSSEQLAFFIRQFETSHLPWVQQLTLLVPSQRIATLAHQLGFHRIVVTLSAANRD
LVAALMPEKPTGTLSNDQQQ

Specific function: Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III [H]

COG id: COG1587

COG function: function code H; Uroporphyrinogen-III synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uroporphyrinogen-III synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790236, Length=247, Percent_Identity=41.2955465587045, Blast_Score=155, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003754 [H]

Pfam domain/function: PF02602 HEM4 [H]

EC number: =4.2.1.75 [H]

Molecular weight: Translated: 28938; Mature: 28938

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIESVMTVLVTRPDEQGAELCQLLHQQGIPALHHPLLAISASPQLAALAEDLSSFDIIIA
CHHHHEEHEEECCCHHHHHHHHHHHHCCCCHHHCCEEEEECCCHHHHHHHHHCCCEEEEE
VSQHAVIFSDQFLQHRRICWPNNAIYLAVGQKTAHVFSKACQQSVDYPEISDSEHLLALS
ECCCEEEECHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
MLRDVAGKKVLILRGNGGRELIYSTLVERGATVRYQEAYCRHERPFAAAECVQKWQLAKV
HHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEHHHHHHHCCCCCHHHHHHHHHHHHHH
DSLVITSSEQLAFFIRQFETSHLPWVQQLTLLVPSQRIATLAHQLGFHRIVVTLSAANRD
CEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHCCEEEEEEEECCCCC
LVAALMPEKPTGTLSNDQQQ
EEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MIESVMTVLVTRPDEQGAELCQLLHQQGIPALHHPLLAISASPQLAALAEDLSSFDIIIA
CHHHHEEHEEECCCHHHHHHHHHHHHCCCCHHHCCEEEEECCCHHHHHHHHHCCCEEEEE
VSQHAVIFSDQFLQHRRICWPNNAIYLAVGQKTAHVFSKACQQSVDYPEISDSEHLLALS
ECCCEEEECHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
MLRDVAGKKVLILRGNGGRELIYSTLVERGATVRYQEAYCRHERPFAAAECVQKWQLAKV
HHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEHHHHHHHCCCCCHHHHHHHHHHHHHH
DSLVITSSEQLAFFIRQFETSHLPWVQQLTLLVPSQRIATLAHQLGFHRIVVTLSAANRD
CEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHCCEEEEEEEECCCCC
LVAALMPEKPTGTLSNDQQQ
EEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3054815; 3320969; 3277628; 3040684; 1379743; 9278503 [H]