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The map label for this gene is hemX [H]

Identifier: 227080354

GI number: 227080354

Start: 112715

End: 113914

Strand: Reverse

Name: hemX [H]

Synonym: VCM66_0118

Alternate gene names: 227080354

Gene position: 113914-112715 (Counterclockwise)

Preceding gene: 227080355

Following gene: 227080353

Centisome position: 3.94

GC content: 52.0

Gene sequence:

>1200_bases
ATGACCAACAACAATAAACCGCAACAAGAACCTGAAATCACAGTAAACCCAAGCTCGGCAGCGGTGACTCCCCCGACCGC
GCCCGACAAACAAGATAGCCCAGCGCCAAACCAACCGGCGGCCAAGAAAACCTTGGACAGCACACCAGCTCAAGACAAGC
CGCCACAACCAAACTCGCAAGGGAAAAAGTTGGCGAGCGTGGCGCTTTTGCTGGTATTAGCGCTCGGCGCAGGTTTTGCC
TATGTGCATCAGCAGCAAAAAAGTGAATTTAACAACCAGTTGCAAGCCGTACGCGCAGAGCTGAAACAGACTCGTGATGC
GCTCAATGCGCAGTTGGAACAAACGGTATCGAAAGCCACGTCACAAGCCACTGAGATTACTCATCGCGCAGAAACCGTGC
TGGAACAGCAACAAAAAAGTATTGAAAGCTTGCAGCTCGCCGTGGCGGATATTAAAGGCCGTCGCCCAAATGATTGGCTA
CTTGCAGAAGCGGATTACTTGGTCAAGCTCGCGGGTCGCAAGCTGTTTCTTGAGCACGATGTGGAAACCGCAACTCAATT
GATGGAAAGTGCCGATCAGCGCATCGCTGCGCTTAATGACCCTAGCCTCACGGCGCTGCGTAAAGCGATGGCCAATGACA
TCACTACCCTGAAAAATATCCCGCTCATTGACCGCGATGGCTTAGTGCTGCGCCTCATCAGCTTGCAACAGCAGATCGAT
AGCTTACCGCTGGCCAATGCCATCTTGCCTGCCGATCAGCCACAAACAAGCCAAGCGGTATCAGAAAACATCGATGACTG
GCAAACCAACCTGAAAAATTCGCTCAAAGCGTTTGCCGATAACTTCATCACTTTCCGCTCACGTGATGGCAATGTGATCC
CTCTTCTCTCTCCTACTCAGCATTTTTATTTGCGAGAGAATCTGCAGGCCAAGTTAGAAACGGCGATCAAAGGGGTGTAC
ACGGAACAACAAACACTCTATCGCATCGCTCTCGACACAGCCGCCCAGTGGTCAACATCGTTCTTCAATCCAGACGATAA
AGCCGTACAAAGCTTTAACGCAGCGTTGGCCCAGCTCGCCGAGCAGCAAGTGCAAGTGGAATACCCCGTTAAATTACAGG
CTCAGCAACAGCTCGCCGATGTGATCAACGAACGCTTGCGCCGCAGTGTTTCACCACTAACCACGGGGGCTGAGCAATGA

Upstream 100 bases:

>100_bases
CACCAACTAGGATTTCATCGCATCGTGGTCACGCTAAGTGCTGCTAATCGCGATTTAGTCGCGGCATTGATGCCCGAAAA
ACCAACAGGAACTCTGAGCA

Downstream 100 bases:

>100_bases
TTCGTCTCATATTCCTCTTTGTTGTCTTAGGACTTGGCCTGTTTGTGGGAACGCAATACGCTGGCCAACAAGGCTATGTG
CTGATCTCGATCGCCAATCG

Product: putative uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen-III methylase; ORF X [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTNNNKPQQEPEITVNPSSAAVTPPTAPDKQDSPAPNQPAAKKTLDSTPAQDKPPQPNSQGKKLASVALLLVLALGAGFA
YVHQQQKSEFNNQLQAVRAELKQTRDALNAQLEQTVSKATSQATEITHRAETVLEQQQKSIESLQLAVADIKGRRPNDWL
LAEADYLVKLAGRKLFLEHDVETATQLMESADQRIAALNDPSLTALRKAMANDITTLKNIPLIDRDGLVLRLISLQQQID
SLPLANAILPADQPQTSQAVSENIDDWQTNLKNSLKAFADNFITFRSRDGNVIPLLSPTQHFYLRENLQAKLETAIKGVY
TEQQTLYRIALDTAAQWSTSFFNPDDKAVQSFNAALAQLAEQQVQVEYPVKLQAQQQLADVINERLRRSVSPLTTGAEQ

Sequences:

>Translated_399_residues
MTNNNKPQQEPEITVNPSSAAVTPPTAPDKQDSPAPNQPAAKKTLDSTPAQDKPPQPNSQGKKLASVALLLVLALGAGFA
YVHQQQKSEFNNQLQAVRAELKQTRDALNAQLEQTVSKATSQATEITHRAETVLEQQQKSIESLQLAVADIKGRRPNDWL
LAEADYLVKLAGRKLFLEHDVETATQLMESADQRIAALNDPSLTALRKAMANDITTLKNIPLIDRDGLVLRLISLQQQID
SLPLANAILPADQPQTSQAVSENIDDWQTNLKNSLKAFADNFITFRSRDGNVIPLLSPTQHFYLRENLQAKLETAIKGVY
TEQQTLYRIALDTAAQWSTSFFNPDDKAVQSFNAALAQLAEQQVQVEYPVKLQAQQQLADVINERLRRSVSPLTTGAEQ
>Mature_398_residues
TNNNKPQQEPEITVNPSSAAVTPPTAPDKQDSPAPNQPAAKKTLDSTPAQDKPPQPNSQGKKLASVALLLVLALGAGFAY
VHQQQKSEFNNQLQAVRAELKQTRDALNAQLEQTVSKATSQATEITHRAETVLEQQQKSIESLQLAVADIKGRRPNDWLL
AEADYLVKLAGRKLFLEHDVETATQLMESADQRIAALNDPSLTALRKAMANDITTLKNIPLIDRDGLVLRLISLQQQIDS
LPLANAILPADQPQTSQAVSENIDDWQTNLKNSLKAFADNFITFRSRDGNVIPLLSPTQHFYLRENLQAKLETAIKGVYT
EQQTLYRIALDTAAQWSTSFFNPDDKAVQSFNAALAQLAEQQVQVEYPVKLQAQQQLADVINERLRRSVSPLTTGAEQ

Specific function: Siroheme and cobalamin biosynthesis. [C]

COG id: COG2959

COG function: function code H; Uncharacterized enzyme of heme biosynthesis

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790235, Length=301, Percent_Identity=36.8770764119601, Blast_Score=201, Evalue=1e-52,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007470 [H]

Pfam domain/function: PF04375 HemX [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 44024; Mature: 43893

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNNKPQQEPEITVNPSSAAVTPPTAPDKQDSPAPNQPAAKKTLDSTPAQDKPPQPNSQ
CCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHH
GKKLASVALLLVLALGAGFAYVHQQQKSEFNNQLQAVRAELKQTRDALNAQLEQTVSKAT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQATEITHRAETVLEQQQKSIESLQLAVADIKGRRPNDWLLAEADYLVKLAGRKLFLEHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHCCHHEEHHH
VETATQLMESADQRIAALNDPSLTALRKAMANDITTLKNIPLIDRDGLVLRLISLQQQID
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
SLPLANAILPADQPQTSQAVSENIDDWQTNLKNSLKAFADNFITFRSRDGNVIPLLSPTQ
CCCCHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCH
HFYLRENLQAKLETAIKGVYTEQQTLYRIALDTAAQWSTSFFNPDDKAVQSFNAALAQLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
EQQVQVEYPVKLQAQQQLADVINERLRRSVSPLTTGAEQ
HHHHEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TNNNKPQQEPEITVNPSSAAVTPPTAPDKQDSPAPNQPAAKKTLDSTPAQDKPPQPNSQ
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHH
GKKLASVALLLVLALGAGFAYVHQQQKSEFNNQLQAVRAELKQTRDALNAQLEQTVSKAT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQATEITHRAETVLEQQQKSIESLQLAVADIKGRRPNDWLLAEADYLVKLAGRKLFLEHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHCCHHEEHHH
VETATQLMESADQRIAALNDPSLTALRKAMANDITTLKNIPLIDRDGLVLRLISLQQQID
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
SLPLANAILPADQPQTSQAVSENIDDWQTNLKNSLKAFADNFITFRSRDGNVIPLLSPTQ
CCCCHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCH
HFYLRENLQAKLETAIKGVYTEQQTLYRIALDTAAQWSTSFFNPDDKAVQSFNAALAQLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
EQQVQVEYPVKLQAQQQLADVINERLRRSVSPLTTGAEQ
HHHHEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3062586; 3054815; 1379743; 9278503; 9298646 [H]