Click here to switch to the map view.

The map label for this gene is purK [H]

Identifier: 227080288

GI number: 227080288

Start: 48323

End: 49456

Strand: Reverse

Name: purK [H]

Synonym: VCM66_0051

Alternate gene names: 227080288

Gene position: 49456-48323 (Counterclockwise)

Preceding gene: 227080289

Following gene: 227080283

Centisome position: 1.71

GC content: 51.32

Gene sequence:

>1134_bases
ATGCGTGTGTTGGTTCTCGGCGCTGGTCAGCTGGCGCGCATGATGTCGCTCGCCGGAGCACCGCTCAATATTGAAACGAT
CGCTTTTGATGTGGGTAGCGAAAACATTGTGCACCCCTTAACGCAAACTGTGCTTGGGCATGGATTGGAGCAAGCGATTG
AACAAGTCGATGTGATCACCGCTGAGTTTGAACACATTCCGCATCCGATCCTCGATCTCTGTGCACGCAGTGGCAAACTT
TACCCAAGCGCTGAAGCTATCAAAGCTGGCGGCGATCGTCGTTTAGAAAAAGCCTTGCTGGATCGCGCCCAAGTGGCGAA
TGCACGTTATACGATGATCCGCAGCCGAGACGACCTAACCTCAGCCATCGCCGAGATTGGATTGCCTATGGTGCTGAAAA
GTGCACTCGGAGGCTACGATGGAAAAGGCCAATGGCGCTTGAAAGAACCAACGCAGATCGAATCGGTTTGGCAAGAACTT
GCGCAATATCTGGCAGCCAACCCCGAACAAGCAATTGTGGCAGAAGAATTTGTCGCTTTTGATCGCGAAGTGTCACTGGT
CGGCGCACGTAACCTAGCCGGCGATGTTGTGGTGTATCCTTTAGCGGAGAACGTTCATACCCAAGGTGTGTTGAGCCTTT
CTATCGCCATTGATGCTCCTGCGCTACAAGCTCAAGCGGAAGCCATGTTTAAAGCGGTAGCCGAGCAGCTCAATTATGTC
GGTGTATTAGCGCTGGAGTTTTTTGAAGTACAAGGCCAGTTACTGGTCAATGAAATTGCACCACGAGTTCATAACTCCGG
TCACTGGACTCAGCAAGGTGCGGAAACCTGTCAGTTCGAAAACCACTTACGCGCAGTGTGTGGCTTACCGCTGGGTAGCA
CCAAACTGGTTCGTGAGACCGCGATGATTAATATTCTTGGTGAAGATCAGCTGCCCGCAGAAGTATTGGCACTGGAAGGC
TGCCACGTACATTGGTACGGCAAAGCCAAACGCCCGGGACGCAAAATGGGGCATATGAATGTGACCGCTGATTACAGTGG
TGAGTTACAGCGCAAACTGTGTCAATTAGCAACTATGTTAGATGAGAAGGCTTTTCCTGCCGTACACGCCGTAGCAAAGG
AAATTCAGCCTTAA

Upstream 100 bases:

>100_bases
CGGCATTCATAACCCAGAAGTGATGAGTAAAGTGGAAGCTTTCCGCGCTAAGCAGACTCAATCTGTGTTGGATAACCCAA
ATCCGGCTGAGGAGTAACAG

Downstream 100 bases:

>100_bases
TCCATACCTCACTAATCCAATAAAGCCGCGTCTAACGCGGCTTTAACTTTGTATGCGATGAGCATGATGAAGCTAGCATT
CCTGAATATGCCCACATTGA

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MRVLVLGAGQLARMMSLAGAPLNIETIAFDVGSENIVHPLTQTVLGHGLEQAIEQVDVITAEFEHIPHPILDLCARSGKL
YPSAEAIKAGGDRRLEKALLDRAQVANARYTMIRSRDDLTSAIAEIGLPMVLKSALGGYDGKGQWRLKEPTQIESVWQEL
AQYLAANPEQAIVAEEFVAFDREVSLVGARNLAGDVVVYPLAENVHTQGVLSLSIAIDAPALQAQAEAMFKAVAEQLNYV
GVLALEFFEVQGQLLVNEIAPRVHNSGHWTQQGAETCQFENHLRAVCGLPLGSTKLVRETAMINILGEDQLPAEVLALEG
CHVHWYGKAKRPGRKMGHMNVTADYSGELQRKLCQLATMLDEKAFPAVHAVAKEIQP

Sequences:

>Translated_377_residues
MRVLVLGAGQLARMMSLAGAPLNIETIAFDVGSENIVHPLTQTVLGHGLEQAIEQVDVITAEFEHIPHPILDLCARSGKL
YPSAEAIKAGGDRRLEKALLDRAQVANARYTMIRSRDDLTSAIAEIGLPMVLKSALGGYDGKGQWRLKEPTQIESVWQEL
AQYLAANPEQAIVAEEFVAFDREVSLVGARNLAGDVVVYPLAENVHTQGVLSLSIAIDAPALQAQAEAMFKAVAEQLNYV
GVLALEFFEVQGQLLVNEIAPRVHNSGHWTQQGAETCQFENHLRAVCGLPLGSTKLVRETAMINILGEDQLPAEVLALEG
CHVHWYGKAKRPGRKMGHMNVTADYSGELQRKLCQLATMLDEKAFPAVHAVAKEIQP
>Mature_377_residues
MRVLVLGAGQLARMMSLAGAPLNIETIAFDVGSENIVHPLTQTVLGHGLEQAIEQVDVITAEFEHIPHPILDLCARSGKL
YPSAEAIKAGGDRRLEKALLDRAQVANARYTMIRSRDDLTSAIAEIGLPMVLKSALGGYDGKGQWRLKEPTQIESVWQEL
AQYLAANPEQAIVAEEFVAFDREVSLVGARNLAGDVVVYPLAENVHTQGVLSLSIAIDAPALQAQAEAMFKAVAEQLNYV
GVLALEFFEVQGQLLVNEIAPRVHNSGHWTQQGAETCQFENHLRAVCGLPLGSTKLVRETAMINILGEDQLPAEVLALEG
CHVHWYGKAKRPGRKMGHMNVTADYSGELQRKLCQLATMLDEKAFPAVHAVAKEIQP

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=361, Percent_Identity=37.9501385041551, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1788155, Length=305, Percent_Identity=24.5901639344262, Blast_Score=79, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324702, Length=391, Percent_Identity=30.690537084399, Blast_Score=172, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 41030; Mature: 41030

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLVLGAGQLARMMSLAGAPLNIETIAFDVGSENIVHPLTQTVLGHGLEQAIEQVDVIT
CEEEEECCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
AEFEHIPHPILDLCARSGKLYPSAEAIKAGGDRRLEKALLDRAQVANARYTMIRSRDDLT
HHHHHCCCHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCHHEEEECHHHHH
SAIAEIGLPMVLKSALGGYDGKGQWRLKEPTQIESVWQELAQYLAANPEQAIVAEEFVAF
HHHHHCCHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DREVSLVGARNLAGDVVVYPLAENVHTQGVLSLSIAIDAPALQAQAEAMFKAVAEQLNYV
CCCEEEEECCCCCCCEEEEECCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
GVLALEFFEVQGQLLVNEIAPRVHNSGHWTQQGAETCQFENHLRAVCGLPLGSTKLVRET
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
AMINILGEDQLPAEVLALEGCHVHWYGKAKRPGRKMGHMNVTADYSGELQRKLCQLATML
HHEEEECCCCCCHHHHEECCCEEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
DEKAFPAVHAVAKEIQP
HHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MRVLVLGAGQLARMMSLAGAPLNIETIAFDVGSENIVHPLTQTVLGHGLEQAIEQVDVIT
CEEEEECCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
AEFEHIPHPILDLCARSGKLYPSAEAIKAGGDRRLEKALLDRAQVANARYTMIRSRDDLT
HHHHHCCCHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCHHEEEECHHHHH
SAIAEIGLPMVLKSALGGYDGKGQWRLKEPTQIESVWQELAQYLAANPEQAIVAEEFVAF
HHHHHCCHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DREVSLVGARNLAGDVVVYPLAENVHTQGVLSLSIAIDAPALQAQAEAMFKAVAEQLNYV
CCCEEEEECCCCCCCEEEEECCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
GVLALEFFEVQGQLLVNEIAPRVHNSGHWTQQGAETCQFENHLRAVCGLPLGSTKLVRET
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
AMINILGEDQLPAEVLALEGCHVHWYGKAKRPGRKMGHMNVTADYSGELQRKLCQLATML
HHEEEECCCCCCHHHHEECCCEEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
DEKAFPAVHAVAKEIQP
HHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]