Click here to switch to the map view.

The map label for this gene is def

Identifier: 227080283

GI number: 227080283

Start: 44298

End: 44807

Strand: Reverse

Name: def

Synonym: VCM66_0046

Alternate gene names: 227080283

Gene position: 44807-44298 (Counterclockwise)

Preceding gene: 227080288

Following gene: 227080282

Centisome position: 1.55

GC content: 47.84

Gene sequence:

>510_bases
ATGTCTGTATTGCAAGTATTAACATTCCCAGATGATCGACTGCGTACCGTTGCGAAACCGGTCGAACAAGTCACCCCAGA
AATCCAACAAATCGTCGATGATATGTTGGAAACCATGTATGCCGAAGAAGGCATCGGTTTAGCGGCGACCCAAGTCGATA
TTCACCAACGTATCGTGGTGATCGATATTTCTGAAACTCGCGATCAGCCTATGGTGTTGATTAACCCAGAGATCATCGAA
AAGCGCGGTGAAGATGGCATTGAAGAAGGCTGTTTGTCCGTCCCGGGTGCGCGAGCTTTAGTGCCTCGCGCCGCAGAAGT
GACCGTAAAAGCGCTGGATCGCAATGGTCAAGAGTACCAGTTTGATGCAGATGACCTACTGGCTATCTGTGTACAGCACG
AACTCGATCACCTCGCGGGCAAACTGTTTGTTGACTATCTGTCACCACTCAAACGCAACCGCATCAAAGAGAAGCTAGAG
AAAATCAAACGCTTCAATGAGAAAAAATAA

Upstream 100 bases:

>100_bases
AGAAATCACGTCCGGATGCTGTCATTTGGGTCATAAAATGTCTAGAATTGAGCCAACACGCATTAAGCTGTTTCGGCACA
GTTCAACATTTCGAGTGTAT

Downstream 100 bases:

>100_bases
ATTACTGTGAGGTAACCTTGAGCCAATCACTACGTATTGTCTTTGCAGGTACTCCGGATTTCGCCGCCCGTCACTTGGCG
GCGTTATTGTCTTCGGAGCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MSVLQVLTFPDDRLRTVAKPVEQVTPEIQQIVDDMLETMYAEEGIGLAATQVDIHQRIVVIDISETRDQPMVLINPEIIE
KRGEDGIEEGCLSVPGARALVPRAAEVTVKALDRNGQEYQFDADDLLAICVQHELDHLAGKLFVDYLSPLKRNRIKEKLE
KIKRFNEKK

Sequences:

>Translated_169_residues
MSVLQVLTFPDDRLRTVAKPVEQVTPEIQQIVDDMLETMYAEEGIGLAATQVDIHQRIVVIDISETRDQPMVLINPEIIE
KRGEDGIEEGCLSVPGARALVPRAAEVTVKALDRNGQEYQFDADDLLAICVQHELDHLAGKLFVDYLSPLKRNRIKEKLE
KIKRFNEKK
>Mature_168_residues
SVLQVLTFPDDRLRTVAKPVEQVTPEIQQIVDDMLETMYAEEGIGLAATQVDIHQRIVVIDISETRDQPMVLINPEIIEK
RGEDGIEEGCLSVPGARALVPRAAEVTVKALDRNGQEYQFDADDLLAICVQHELDHLAGKLFVDYLSPLKRNRIKEKLEK
IKRFNEKK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=158, Percent_Identity=31.6455696202532, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1789682, Length=168, Percent_Identity=70.2380952380952, Blast_Score=251, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24645728, Length=138, Percent_Identity=31.1594202898551, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF1_VIBCH (Q9KVU3)

Other databases:

- EMBL:   AE003852
- PIR:   A82373
- RefSeq:   NP_229705.1
- PDB:   3FWX
- PDBsum:   3FWX
- ProteinModelPortal:   Q9KVU3
- SMR:   Q9KVU3
- GeneID:   2614445
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0046
- TIGR:   VC_0046
- HOGENOM:   HBG665227
- OMA:   RQLVDDM
- ProtClustDB:   PRK00150
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19147; Mature: 19016

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLQVLTFPDDRLRTVAKPVEQVTPEIQQIVDDMLETMYAEEGIGLAATQVDIHQRIVV
CCCCCEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEHEEEHHHCEEEE
IDISETRDQPMVLINPEIIEKRGEDGIEEGCLSVPGARALVPRAAEVTVKALDRNGQEYQ
EEECCCCCCCEEEECHHHHHHCCCCCHHHHHHCCCCCHHHCCCHHHHHHHHHHCCCCEEE
FDADDLLAICVQHELDHLAGKLFVDYLSPLKRNRIKEKLEKIKRFNEKK
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SVLQVLTFPDDRLRTVAKPVEQVTPEIQQIVDDMLETMYAEEGIGLAATQVDIHQRIVV
CCCCEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEHEEEHHHCEEEE
IDISETRDQPMVLINPEIIEKRGEDGIEEGCLSVPGARALVPRAAEVTVKALDRNGQEYQ
EEECCCCCCCEEEECHHHHHHCCCCCHHHHHHCCCCCHHHCCCHHHHHHHHHHCCCCEEE
FDADDLLAICVQHELDHLAGKLFVDYLSPLKRNRIKEKLEKIKRFNEKK
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301