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The map label for this gene is fmt [H]

Identifier: 227080282

GI number: 227080282

Start: 43333

End: 44280

Strand: Reverse

Name: fmt [H]

Synonym: VCM66_0045

Alternate gene names: 227080282

Gene position: 44280-43333 (Counterclockwise)

Preceding gene: 227080283

Following gene: 227080281

Centisome position: 1.53

GC content: 51.79

Gene sequence:

>948_bases
TTGAGCCAATCACTACGTATTGTCTTTGCAGGTACTCCGGATTTCGCCGCCCGTCACTTGGCGGCGTTATTGTCTTCGGA
GCATGAGATTATTGCGGTCTACACCCAACCGGATCGCCCAGCGGGGCGCGGCAAAAAACTCACCGCCAGCCCAGTCAAAA
CCCTTGCACTTGAGCACAACGTTCCGGTTTATCAGCCAGAGAACTTTAAATCAGACGAGTCTAAACAACAGCTTGCCGCA
CTCAACGCCGATTTAATGGTGGTCGTGGCTTACGGCTTGCTACTGCCTAAAGTAGTTTTGGATACACCTAAACTTGGCTG
TATTAATGTGCACGGTTCGATTCTGCCGCGTTGGCGGGGTGCTGCGCCGATCCAGCGCTCCATTTGGGCGGGTGATAGCG
AAACTGGCGTCACCATCATGCAGATGGATGTCGGCCTTGATACGGGCGATATGCTGAAAATCGCGACGCTGCCGATTGAA
GCGAGCGACACCAGTGCCTCGATGTACGACAAACTGGCAGAACTTGGTCCACAAGCGCTGCTCGAGTGCTTACAAGAGAT
CGCTCAAGGCACAGCGGTTGCGGTCAAACAAGACGATGCGCTGGCCAACTATGCTCATAAGCTTAGTAAAGAAGAAGCCC
GTATTAACTGGAATGATGAAGCCGCCCATATTGAGCGCTGCATTCGCGCCTTTAATCCTTGGCCGATGAGCCATTTTGAA
GTAGCAGAAAACAGCATTAAGGTTTGGCAAGCGCGTGTTGAAACACGAGCAGTAACTCAAACTCCGGGTACCATTATCCA
AGCAGATAAGAGCGGGATTTACGTGGCAACCGGGCAAGATGTACTAGTGCTCGAAAGCCTGCAAATTCCGGGCAAGAAAG
CCTTGCCAGTACAAGATATTCTCAATGCGCGTGCCGACTGGTTTAGTGTCGGTTCCCAACTCAGTTAA

Upstream 100 bases:

>100_bases
TTGTTGACTATCTGTCACCACTCAAACGCAACCGCATCAAAGAGAAGCTAGAGAAAATCAAACGCTTCAATGAGAAAAAA
TAAATTACTGTGAGGTAACC

Downstream 100 bases:

>100_bases
TCCTGAACCCTAGCCTATGGATGGCTAGGGTTTGGCTTTTTGTTTAGGTTTTATTATGAATGTTCGCGCCGCTGCTGCGT
CGGCTCTTTACCAAGTGGTT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSQSLRIVFAGTPDFAARHLAALLSSEHEIIAVYTQPDRPAGRGKKLTASPVKTLALEHNVPVYQPENFKSDESKQQLAA
LNADLMVVVAYGLLLPKVVLDTPKLGCINVHGSILPRWRGAAPIQRSIWAGDSETGVTIMQMDVGLDTGDMLKIATLPIE
ASDTSASMYDKLAELGPQALLECLQEIAQGTAVAVKQDDALANYAHKLSKEEARINWNDEAAHIERCIRAFNPWPMSHFE
VAENSIKVWQARVETRAVTQTPGTIIQADKSGIYVATGQDVLVLESLQIPGKKALPVQDILNARADWFSVGSQLS

Sequences:

>Translated_315_residues
MSQSLRIVFAGTPDFAARHLAALLSSEHEIIAVYTQPDRPAGRGKKLTASPVKTLALEHNVPVYQPENFKSDESKQQLAA
LNADLMVVVAYGLLLPKVVLDTPKLGCINVHGSILPRWRGAAPIQRSIWAGDSETGVTIMQMDVGLDTGDMLKIATLPIE
ASDTSASMYDKLAELGPQALLECLQEIAQGTAVAVKQDDALANYAHKLSKEEARINWNDEAAHIERCIRAFNPWPMSHFE
VAENSIKVWQARVETRAVTQTPGTIIQADKSGIYVATGQDVLVLESLQIPGKKALPVQDILNARADWFSVGSQLS
>Mature_314_residues
SQSLRIVFAGTPDFAARHLAALLSSEHEIIAVYTQPDRPAGRGKKLTASPVKTLALEHNVPVYQPENFKSDESKQQLAAL
NADLMVVVAYGLLLPKVVLDTPKLGCINVHGSILPRWRGAAPIQRSIWAGDSETGVTIMQMDVGLDTGDMLKIATLPIEA
SDTSASMYDKLAELGPQALLECLQEIAQGTAVAVKQDDALANYAHKLSKEEARINWNDEAAHIERCIRAFNPWPMSHFEV
AENSIKVWQARVETRAVTQTPGTIIQADKSGIYVATGQDVLVLESLQIPGKKALPVQDILNARADWFSVGSQLS

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI164663775, Length=329, Percent_Identity=30.3951367781155, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI21614513, Length=318, Percent_Identity=25.1572327044025, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI238814322, Length=325, Percent_Identity=26.1538461538462, Blast_Score=106, Evalue=2e-23,
Organism=Escherichia coli, GI1789683, Length=314, Percent_Identity=64.6496815286624, Blast_Score=426, Evalue=1e-121,
Organism=Escherichia coli, GI1788589, Length=261, Percent_Identity=29.5019157088123, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI133930964, Length=319, Percent_Identity=26.3322884012539, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319458, Length=204, Percent_Identity=32.3529411764706, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI45550868, Length=303, Percent_Identity=29.042904290429, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28571984, Length=225, Percent_Identity=31.1111111111111, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585660, Length=266, Percent_Identity=30.4511278195489, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 34270; Mature: 34138

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSLRIVFAGTPDFAARHLAALLSSEHEIIAVYTQPDRPAGRGKKLTASPVKTLALEHN
CCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEECCHHHHEEEECC
VPVYQPENFKSDESKQQLAALNADLMVVVAYGLLLPKVVLDTPKLGCINVHGSILPRWRG
CCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
AAPIQRSIWAGDSETGVTIMQMDVGLDTGDMLKIATLPIEASDTSASMYDKLAELGPQAL
CCCCHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCHHHH
LECLQEIAQGTAVAVKQDDALANYAHKLSKEEARINWNDEAAHIERCIRAFNPWPMSHFE
HHHHHHHHCCCEEEEECCHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHCCCCCCCHHH
VAENSIKVWQARVETRAVTQTPGTIIQADKSGIYVATGQDVLVLESLQIPGKKALPVQDI
HHHHHHHHHHHHHHHHHEECCCCCEEEECCCCEEEECCCCEEEEEECCCCCCCCCCHHHH
LNARADWFSVGSQLS
HHCCHHHHHCCCCCC
>Mature Secondary Structure 
SQSLRIVFAGTPDFAARHLAALLSSEHEIIAVYTQPDRPAGRGKKLTASPVKTLALEHN
CCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEECCHHHHEEEECC
VPVYQPENFKSDESKQQLAALNADLMVVVAYGLLLPKVVLDTPKLGCINVHGSILPRWRG
CCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
AAPIQRSIWAGDSETGVTIMQMDVGLDTGDMLKIATLPIEASDTSASMYDKLAELGPQAL
CCCCHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCHHHH
LECLQEIAQGTAVAVKQDDALANYAHKLSKEEARINWNDEAAHIERCIRAFNPWPMSHFE
HHHHHHHHCCCEEEEECCHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHCCCCCCCHHH
VAENSIKVWQARVETRAVTQTPGTIIQADKSGIYVATGQDVLVLESLQIPGKKALPVQDI
HHHHHHHHHHHHHHHHHEECCCCCEEEECCCCEEEECCCCEEEEEECCCCCCCCCCHHHH
LNARADWFSVGSQLS
HHCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA