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The map label for this gene is ilvD

Identifier: 227080265

GI number: 227080265

Start: 24792

End: 26633

Strand: Reverse

Name: ilvD

Synonym: VCM66_0028

Alternate gene names: 227080265

Gene position: 26633-24792 (Counterclockwise)

Preceding gene: 227080266

Following gene: 227080264

Centisome position: 0.92

GC content: 54.72

Gene sequence:

>1842_bases
ATGCCTAAGTACCGTTCCGCCACCACCACACACGGACGTAACATGGCGGGTGCGCGCGCATTATGGCGCGCCACCGGCGT
CAAAGAGGAAGATTTTGGTAAACCGATCATTGCGGTGGTCAACTCCTTCACCCAATTTGTTCCCGGCCATGTGCATTTAA
AAGATCTCGGCCAACTGGTCGCACGCGAAATTGAAGCCGCGGGCGGCATTGCCAAAGAATTCAACACCATTGCGGTCGAT
GACGGCATCGCCATGGGCCACGGCGGCATGCTCTACTCGCTGCCATCGCGTGAACTGATCGCCGATTCCGTCGAATACAT
GGTCAATGCGCACTGCGCCGATGCCATGGTGTGTATCTCCAACTGTGACAAAATCACTCCAGGAATGCTGATGGCGGCGA
TGCGCCTTAATATTCCGGCGATTTTCGTCTCTGGCGGCCCGATGGAAGCGGGAAAAACCAAGCTTTCTGACCAAATCATC
AAGCTCGACTTGGTCGATGCCATGATGCAAGGCGCTGACCCCAAAGTATCGGATGCCCAAAGTGAGCAGATTGAACGCTC
GGCCTGTCCTACTTGTGGCTCTTGCTCGGGGATGTTTACCGCCAACTCAATGAACTGCTTGACCGAAGCGCTGGGTCTCT
CTCAGCCGGGTAACGGCTCACTGCTCGCCACGCATGCCGATCGCAAACAGCTGTTTTTGACTGCTGGGCAACGGATTGTT
GAGCTGACTAAACGTTACTACGAGCAAGATGATGCCTCAGTTTTGCCACGCAACATTGCCAACAAAGCAGCGTTTGAAAA
CGCCATTGCGCTGGATATTGCGATGGGCGGCTCCACCAACACGGTGTTACACCTGTTGGCCGCTGCGCAGGAAGGCGAAG
TGGAATTTGATATGACCGATATCGATCGCATGTCTCGCCAAGTGCCACACTTATGTAAAGTCGCGCCTTCCACTCAGAAA
TACCATATGGAAGATGTACACCGCGCGGGCGGTGTAATGGGCATTTTGGGTGAGCTACAACGTGCAGGTCTACTCAAGGA
TCAAACCCGCACCGTACTAGGCATCTCGCTGCAAGAGCAGCTGGCGCAATACGACGTGAAACAAACCCAAGATCCAGCGG
TACATACTATGTTCCGCGCAGGCCCTGCTGGCATTCGCACCACTCAAGCTTTCTCACAAGATTGCCGTTGGGATACGCTC
GATGACGATCGCCAAGAGGGGTGTATTCGCGATAAAGCGCACGCATTCAGTCAAGATGGTGGCCTTGCCGTACTCAAAGG
CAACTTGGCGATTGACGGCTGCATCGTAAAAACTGCTGGCGTGGATGAAAGTATCCTCAAATTCCGTGGTCCTGCTGTGG
TGTATGAAAGTCAGGAAGACGCGGTCAACGGCATTCTTGGCGGCCAAGTCAAAGCCGGTGACGTCGTGGTTATTCGTTAT
GAAGGGCCAAAAGGCGGTCCGGGCATGCAAGAGATGCTCTACCCAACCACCTACCTCAAATCGATGGGACTGGGTAAACA
GTGCGCGCTACTGACCGATGGCCGCTTCTCCGGTGGTACGTCCGGCTTGTCGATTGGTCACGCCTCTCCGGAAGCGGCGA
ATGGCGGTACGATCGGCTTGGTACGCAGTGGCGATAGCATCGCGATTGATATTCCCAACCGTTCGATCACGCTCGAAGTC
AGCGAATCTGAGCTAGCAGCTCGCCGCGCTGAACAAGATAAACTCGGCTGGAAACCAGTGGATCGCCAGCGTACCGTTTC
TTTGGCGCTGAAAGCTTATGCCAGCATGGCAACCAGCGCCGACAAAGGTGCAGTGCGCGATAAGTCCAAACTTGAGGGCT
AA

Upstream 100 bases:

>100_bases
GGTAGTACAAGATGCTTACTTTGGTCTGTTCAAAGGCACCACAGAAGATAAGTGGGGCTGGTTGGATTACGTTTACCCAG
CAGCGAAGTAAGGAGTTTCC

Downstream 100 bases:

>100_bases
GCCATGGGGTTAACCAATCAAACTGGCGCAGAGTATCTGCGCCAGATCTTGCGCTCTCCGGTGTATGAGGTAGCGAACGT
CACGCCATTGCAAACCATGC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MPKYRSATTTHGRNMAGARALWRATGVKEEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGIAKEFNTIAVD
DGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPAIFVSGGPMEAGKTKLSDQII
KLDLVDAMMQGADPKVSDAQSEQIERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFLTAGQRIV
ELTKRYYEQDDASVLPRNIANKAAFENAIALDIAMGGSTNTVLHLLAAAQEGEVEFDMTDIDRMSRQVPHLCKVAPSTQK
YHMEDVHRAGGVMGILGELQRAGLLKDQTRTVLGISLQEQLAQYDVKQTQDPAVHTMFRAGPAGIRTTQAFSQDCRWDTL
DDDRQEGCIRDKAHAFSQDGGLAVLKGNLAIDGCIVKTAGVDESILKFRGPAVVYESQEDAVNGILGGQVKAGDVVVIRY
EGPKGGPGMQEMLYPTTYLKSMGLGKQCALLTDGRFSGGTSGLSIGHASPEAANGGTIGLVRSGDSIAIDIPNRSITLEV
SESELAARRAEQDKLGWKPVDRQRTVSLALKAYASMATSADKGAVRDKSKLEG

Sequences:

>Translated_613_residues
MPKYRSATTTHGRNMAGARALWRATGVKEEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGIAKEFNTIAVD
DGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPAIFVSGGPMEAGKTKLSDQII
KLDLVDAMMQGADPKVSDAQSEQIERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFLTAGQRIV
ELTKRYYEQDDASVLPRNIANKAAFENAIALDIAMGGSTNTVLHLLAAAQEGEVEFDMTDIDRMSRQVPHLCKVAPSTQK
YHMEDVHRAGGVMGILGELQRAGLLKDQTRTVLGISLQEQLAQYDVKQTQDPAVHTMFRAGPAGIRTTQAFSQDCRWDTL
DDDRQEGCIRDKAHAFSQDGGLAVLKGNLAIDGCIVKTAGVDESILKFRGPAVVYESQEDAVNGILGGQVKAGDVVVIRY
EGPKGGPGMQEMLYPTTYLKSMGLGKQCALLTDGRFSGGTSGLSIGHASPEAANGGTIGLVRSGDSIAIDIPNRSITLEV
SESELAARRAEQDKLGWKPVDRQRTVSLALKAYASMATSADKGAVRDKSKLEG
>Mature_612_residues
PKYRSATTTHGRNMAGARALWRATGVKEEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGIAKEFNTIAVDD
GIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPAIFVSGGPMEAGKTKLSDQIIK
LDLVDAMMQGADPKVSDAQSEQIERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFLTAGQRIVE
LTKRYYEQDDASVLPRNIANKAAFENAIALDIAMGGSTNTVLHLLAAAQEGEVEFDMTDIDRMSRQVPHLCKVAPSTQKY
HMEDVHRAGGVMGILGELQRAGLLKDQTRTVLGISLQEQLAQYDVKQTQDPAVHTMFRAGPAGIRTTQAFSQDCRWDTLD
DDRQEGCIRDKAHAFSQDGGLAVLKGNLAIDGCIVKTAGVDESILKFRGPAVVYESQEDAVNGILGGQVKAGDVVVIRYE
GPKGGPGMQEMLYPTTYLKSMGLGKQCALLTDGRFSGGTSGLSIGHASPEAANGGTIGLVRSGDSIAIDIPNRSITLEVS
ESELAARRAEQDKLGWKPVDRQRTVSLALKAYASMATSADKGAVRDKSKLEG

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=616, Percent_Identity=83.2792207792208, Blast_Score=1073, Evalue=0.0,
Organism=Escherichia coli, GI1788157, Length=577, Percent_Identity=28.7694974003466, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI2367371, Length=559, Percent_Identity=28.9803220035778, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1786464, Length=536, Percent_Identity=28.544776119403, Blast_Score=158, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6322476, Length=612, Percent_Identity=38.2352941176471, Blast_Score=348, Evalue=1e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_VIBC3 (A5F497)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- STRING:   A5F497
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2491
- eggNOG:   COG0129
- HOGENOM:   HBG671001
- OMA:   KVPCLSK
- ProtClustDB:   PRK12448
- BioCyc:   VCHO345073:VC0395_A2491-MONOMER
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 65437; Mature: 65306

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKYRSATTTHGRNMAGARALWRATGVKEEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLV
CCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AREIEAAGGIAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCIS
HHHHHHHCCHHHHCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMAAMRLNIPAIFVSGGPMEAGKTKLSDQIIKLDLVDAMMQGADPKVSDAQ
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCH
SEQIERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFLTAGQRIV
HHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHH
ELTKRYYEQDDASVLPRNIANKAAFENAIALDIAMGGSTNTVLHLLAAAQEGEVEFDMTD
HHHHHHHCCCCHHHCCHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHCCCCCEEECHHH
IDRMSRQVPHLCKVAPSTQKYHMEDVHRAGGVMGILGELQRAGLLKDQTRTVLGISLQEQ
HHHHHHHCCHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCHHCCHHEEEECHHHH
LAQYDVKQTQDPAVHTMFRAGPAGIRTTQAFSQDCRWDTLDDDRQEGCIRDKAHAFSQDG
HHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHCCCC
GLAVLKGNLAIDGCIVKTAGVDESILKFRGPAVVYESQEDAVNGILGGQVKAGDVVVIRY
CEEEEECCEEEEEEEEEECCCCHHHHHHCCCEEEEECCHHHHHCCCCCCEECCCEEEEEE
EGPKGGPGMQEMLYPTTYLKSMGLGKQCALLTDGRFSGGTSGLSIGHASPEAANGGTIGL
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEE
VRSGDSIAIDIPNRSITLEVSESELAARRAEQDKLGWKPVDRQRTVSLALKAYASMATSA
EECCCEEEEECCCCEEEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
DKGAVRDKSKLEG
CCCCCCCHHHCCC
>Mature Secondary Structure 
PKYRSATTTHGRNMAGARALWRATGVKEEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLV
CCCCCCCCCCCCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AREIEAAGGIAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCIS
HHHHHHHCCHHHHCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMAAMRLNIPAIFVSGGPMEAGKTKLSDQIIKLDLVDAMMQGADPKVSDAQ
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCH
SEQIERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFLTAGQRIV
HHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHH
ELTKRYYEQDDASVLPRNIANKAAFENAIALDIAMGGSTNTVLHLLAAAQEGEVEFDMTD
HHHHHHHCCCCHHHCCHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHCCCCCEEECHHH
IDRMSRQVPHLCKVAPSTQKYHMEDVHRAGGVMGILGELQRAGLLKDQTRTVLGISLQEQ
HHHHHHHCCHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCHHCCHHEEEECHHHH
LAQYDVKQTQDPAVHTMFRAGPAGIRTTQAFSQDCRWDTLDDDRQEGCIRDKAHAFSQDG
HHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHCCCC
GLAVLKGNLAIDGCIVKTAGVDESILKFRGPAVVYESQEDAVNGILGGQVKAGDVVVIRY
CEEEEECCEEEEEEEEEECCCCHHHHHHCCCEEEEECCHHHHHCCCCCCEECCCEEEEEE
EGPKGGPGMQEMLYPTTYLKSMGLGKQCALLTDGRFSGGTSGLSIGHASPEAANGGTIGL
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEE
VRSGDSIAIDIPNRSITLEVSESELAARRAEQDKLGWKPVDRQRTVSLALKAYASMATSA
EECCCEEEEECCCCEEEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
DKGAVRDKSKLEG
CCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA