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The map label for this gene is ilvA [H]

Identifier: 227080264

GI number: 227080264

Start: 23256

End: 24788

Strand: Reverse

Name: ilvA [H]

Synonym: VCM66_0027

Alternate gene names: 227080264

Gene position: 24788-23256 (Counterclockwise)

Preceding gene: 227080265

Following gene: 227080263

Centisome position: 0.86

GC content: 52.32

Gene sequence:

>1533_bases
ATGGGGTTAACCAATCAAACTGGCGCAGAGTATCTGCGCCAGATCTTGCGCTCTCCGGTGTATGAGGTAGCGAACGTCAC
GCCATTGCAAACCATGCCGCGTCTTTCGGCGCGGATTGGTAACCAAGTGCAGATCAAACGTGAAGATCGCCAGCCAGTAC
ACTCGTTTAAACTGCGCGGCGCTTACAACATGGTGTCGCACCTAACCGAAGCACAAAAAGCCGCGGGCGTAATTGCCGCT
TCCGCAGGTAATCATGCCCAAGGTATGGCGCTGTCAGGAACGAAACTTGGCATCAAAACCACCATCGTCATGCCACGCAC
CACGCCAGACATTAAAGTTGAAGCGGTACGCGGATTTGGCGGCGAAGTACTGCTGCATGGCAGCAATTTCGATGAAGCCA
AAGCCGAAGCCGAACGTTTATCCAAAGAGCAAGGCTACACCTTTGTACCTCCGTTCGATCACCCTTTGGTGATTGCCGGT
CAAGGCACGATTGGCATGGAAATGCTGCAGCAAAACGGTCATTTGGATTACATTTTTGTGCCGGTCGGTGGCGGCGGTTT
AGCGGCGGGAGTGGCGGTTCTGGTCAAACAGTTGATGCCGGAGATCCAAGTCATCGCGGTTGAACCGGAAGACTCAGCCT
GCCTCAAAGCAGCCTTAGATGCGGGTAAACCTGTGGTGCTGGATCAAGTCAGCATGTTTGCTGATGGTGTGGCAGTAAAA
CGAATTGGCGATGAAACCTTTCGCCTTTGCCAGCAATACATTGACGGCCACGTGACGGTGTCCAGTGATGAAATCTGCGC
GGCGGTGAAAGACATTTTTGAAGATACGCGCGCCATCGCTGAACCTTCTGGCGCATTGGCGCTGGCGGGACTAAAGAAAT
TTGCCGAACAGCAGCAGCTCAAAGGCAAACAGTTGGGTACGGTACTTTCTGGCGCCAACACCAACTTCCACGGTTTGCGT
TATGTGTCGGAGCGCTGCGAACTGGGTGAAAAACGCGAAGGCTTACTGGCGGTGACCATTCCAGAGCGCAAAGGGGCGTT
TTTCGACTTCTGCCAAATTATTGGTAACCGCGCCGTCACCGAATTTAACTACCGTTACAATGATGATCAACTGGCCAATA
TTTTTGTCGGTGTGCGTTTAGTCGGCGGCCCAGATGAGCTGAAATCGATCATTCACGAGCTGCGCCAATCCGGCTATCCG
GTGCAAGATCTCTCGGATGATGAAATGGCCAAGCTACACATTCGCTACATGATTGGTGGTCGCCCTTCCAAACCACTGAC
CGAGCGTTTGTACAGCTTTGAGTTTCCTGAATACCCCGGCGCACTGCTCAAATTCTTGAGCATGCTCGGCACCCACTGGA
ACATCAGCTTGTTCAACTACCGCAACCACGGCGCAGACTACGGCCGCGTGCTGTGTGGTTTTGAACTGGATACACCAGAC
CTAGTGCGCTTTTCAGAGCATTTGGTTGAACTCGGTTATCGCTATAAAGATGAAACCGATAACCCTGCGTATCGTTTCTT
CCTAGCTAAATAA

Upstream 100 bases:

>100_bases
CCAGCGTACCGTTTCTTTGGCGCTGAAAGCTTATGCCAGCATGGCAACCAGCGCCGACAAAGGTGCAGTGCGCGATAAGT
CCAAACTTGAGGGCTAAGCC

Downstream 100 bases:

>100_bases
TCTTCGCCCCACCTTATGTGGGGCTCTTTCTCTTTACCGGCGAGCCATCAGCATCGAGCGAAGTGGCCAAGGTCAAGTGT
TGAAAAATCAACCATTTCAT

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MGLTNQTGAEYLRQILRSPVYEVANVTPLQTMPRLSARIGNQVQIKREDRQPVHSFKLRGAYNMVSHLTEAQKAAGVIAA
SAGNHAQGMALSGTKLGIKTTIVMPRTTPDIKVEAVRGFGGEVLLHGSNFDEAKAEAERLSKEQGYTFVPPFDHPLVIAG
QGTIGMEMLQQNGHLDYIFVPVGGGGLAAGVAVLVKQLMPEIQVIAVEPEDSACLKAALDAGKPVVLDQVSMFADGVAVK
RIGDETFRLCQQYIDGHVTVSSDEICAAVKDIFEDTRAIAEPSGALALAGLKKFAEQQQLKGKQLGTVLSGANTNFHGLR
YVSERCELGEKREGLLAVTIPERKGAFFDFCQIIGNRAVTEFNYRYNDDQLANIFVGVRLVGGPDELKSIIHELRQSGYP
VQDLSDDEMAKLHIRYMIGGRPSKPLTERLYSFEFPEYPGALLKFLSMLGTHWNISLFNYRNHGADYGRVLCGFELDTPD
LVRFSEHLVELGYRYKDETDNPAYRFFLAK

Sequences:

>Translated_510_residues
MGLTNQTGAEYLRQILRSPVYEVANVTPLQTMPRLSARIGNQVQIKREDRQPVHSFKLRGAYNMVSHLTEAQKAAGVIAA
SAGNHAQGMALSGTKLGIKTTIVMPRTTPDIKVEAVRGFGGEVLLHGSNFDEAKAEAERLSKEQGYTFVPPFDHPLVIAG
QGTIGMEMLQQNGHLDYIFVPVGGGGLAAGVAVLVKQLMPEIQVIAVEPEDSACLKAALDAGKPVVLDQVSMFADGVAVK
RIGDETFRLCQQYIDGHVTVSSDEICAAVKDIFEDTRAIAEPSGALALAGLKKFAEQQQLKGKQLGTVLSGANTNFHGLR
YVSERCELGEKREGLLAVTIPERKGAFFDFCQIIGNRAVTEFNYRYNDDQLANIFVGVRLVGGPDELKSIIHELRQSGYP
VQDLSDDEMAKLHIRYMIGGRPSKPLTERLYSFEFPEYPGALLKFLSMLGTHWNISLFNYRNHGADYGRVLCGFELDTPD
LVRFSEHLVELGYRYKDETDNPAYRFFLAK
>Mature_509_residues
GLTNQTGAEYLRQILRSPVYEVANVTPLQTMPRLSARIGNQVQIKREDRQPVHSFKLRGAYNMVSHLTEAQKAAGVIAAS
AGNHAQGMALSGTKLGIKTTIVMPRTTPDIKVEAVRGFGGEVLLHGSNFDEAKAEAERLSKEQGYTFVPPFDHPLVIAGQ
GTIGMEMLQQNGHLDYIFVPVGGGGLAAGVAVLVKQLMPEIQVIAVEPEDSACLKAALDAGKPVVLDQVSMFADGVAVKR
IGDETFRLCQQYIDGHVTVSSDEICAAVKDIFEDTRAIAEPSGALALAGLKKFAEQQQLKGKQLGTVLSGANTNFHGLRY
VSERCELGEKREGLLAVTIPERKGAFFDFCQIIGNRAVTEFNYRYNDDQLANIFVGVRLVGGPDELKSIIHELRQSGYPV
QDLSDDEMAKLHIRYMIGGRPSKPLTERLYSFEFPEYPGALLKFLSMLGTHWNISLFNYRNHGADYGRVLCGFELDTPDL
VRFSEHLVELGYRYKDETDNPAYRFFLAK

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia. Deaminates L-threonine, but also L-serine to a lesser extent [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=297, Percent_Identity=33.3333333333333, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI19923959, Length=272, Percent_Identity=33.8235294117647, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI33469958, Length=317, Percent_Identity=29.0220820189274, Blast_Score=103, Evalue=6e-22,
Organism=Escherichia coli, GI1790207, Length=504, Percent_Identity=70.6349206349206, Blast_Score=711, Evalue=0.0,
Organism=Escherichia coli, GI1789505, Length=295, Percent_Identity=38.3050847457627, Blast_Score=184, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI71991565, Length=344, Percent_Identity=34.0116279069767, Blast_Score=166, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17537387, Length=321, Percent_Identity=34.5794392523364, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17508781, Length=269, Percent_Identity=34.2007434944238, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17535051, Length=327, Percent_Identity=24.4648318042813, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6320930, Length=522, Percent_Identity=48.6590038314176, Blast_Score=468, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6322631, Length=304, Percent_Identity=36.8421052631579, Blast_Score=193, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6319788, Length=288, Percent_Identity=25, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355833, Length=343, Percent_Identity=34.1107871720117, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24645328, Length=279, Percent_Identity=31.1827956989247, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 56174; Mature: 56043

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTNQTGAEYLRQILRSPVYEVANVTPLQTMPRLSARIGNQVQIKREDRQPVHSFKLRG
CCCCCCHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCC
AYNMVSHLTEAQKAAGVIAASAGNHAQGMALSGTKLGIKTTIVMPRTTPDIKVEAVRGFG
HHHHHHHHHHHHHHCCEEEECCCCCCCCEEECCCEECEEEEEEECCCCCCCEEEEEECCC
GEVLLHGSNFDEAKAEAERLSKEQGYTFVPPFDHPLVIAGQGTIGMEMLQQNGHLDYIFV
CEEEEECCCCCHHHHHHHHHHHHCCCEECCCCCCCEEEECCCCHHHHHHHCCCCEEEEEE
PVGGGGLAAGVAVLVKQLMPEIQVIAVEPEDSACLKAALDAGKPVVLDQVSMFADGVAVK
EECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEHHHHHHHHCCHHHH
RIGDETFRLCQQYIDGHVTVSSDEICAAVKDIFEDTRAIAEPSGALALAGLKKFAEQQQL
HCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
KGKQLGTVLSGANTNFHGLRYVSERCELGEKREGLLAVTIPERKGAFFDFCQIIGNRAVT
CHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEE
EFNYRYNDDQLANIFVGVRLVGGPDELKSIIHELRQSGYPVQDLSDDEMAKLHIRYMIGG
EEECEECCHHHEEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECC
RPSKPLTERLYSFEFPEYPGALLKFLSMLGTHWNISLFNYRNHGADYGRVLCGFELDTPD
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEEEECCCHH
LVRFSEHLVELGYRYKDETDNPAYRFFLAK
HHHHHHHHHHHCCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure 
GLTNQTGAEYLRQILRSPVYEVANVTPLQTMPRLSARIGNQVQIKREDRQPVHSFKLRG
CCCCCHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCC
AYNMVSHLTEAQKAAGVIAASAGNHAQGMALSGTKLGIKTTIVMPRTTPDIKVEAVRGFG
HHHHHHHHHHHHHHCCEEEECCCCCCCCEEECCCEECEEEEEEECCCCCCCEEEEEECCC
GEVLLHGSNFDEAKAEAERLSKEQGYTFVPPFDHPLVIAGQGTIGMEMLQQNGHLDYIFV
CEEEEECCCCCHHHHHHHHHHHHCCCEECCCCCCCEEEECCCCHHHHHHHCCCCEEEEEE
PVGGGGLAAGVAVLVKQLMPEIQVIAVEPEDSACLKAALDAGKPVVLDQVSMFADGVAVK
EECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEHHHHHHHHCCHHHH
RIGDETFRLCQQYIDGHVTVSSDEICAAVKDIFEDTRAIAEPSGALALAGLKKFAEQQQL
HCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
KGKQLGTVLSGANTNFHGLRYVSERCELGEKREGLLAVTIPERKGAFFDFCQIIGNRAVT
CHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEE
EFNYRYNDDQLANIFVGVRLVGGPDELKSIIHELRQSGYPVQDLSDDEMAKLHIRYMIGG
EEECEECCHHHEEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECC
RPSKPLTERLYSFEFPEYPGALLKFLSMLGTHWNISLFNYRNHGADYGRVLCGFELDTPD
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEEEECCCHH
LVRFSEHLVELGYRYKDETDNPAYRFFLAK
HHHHHHHHHHHCCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3315862; 3550695; 1379743; 9278503; 3003115; 2473940; 9562556 [H]