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The map label for this gene is ilvE [H]

Identifier: 227080266

GI number: 227080266

Start: 26643

End: 27602

Strand: Reverse

Name: ilvE [H]

Synonym: VCM66_0029

Alternate gene names: 227080266

Gene position: 27602-26643 (Counterclockwise)

Preceding gene: 227080267

Following gene: 227080265

Centisome position: 0.95

GC content: 53.65

Gene sequence:

>960_bases
TTGCGCATAAGGAAGGCAATCATGGCAACAAAAACTGCTGATTATATTTGGTTCAATGGCGAGATGATCCCATGGGCGAA
TGCGACCGTTCACGTACTCACCCATGCAATGCACTACGGCACTTCGGTGTTTGAAGGTGTTCGCTGCTACAACACCCCAA
AAGGCCCTATCATCTTCCGCCATCGCGAACACGCGCAGCGCCTGCTTGATTCAGCCAAAATCTACCGCTTTCCAATCCCT
TATTCGATCGAGACCATCATGGAAGCGACGCGCGAAACCTTACGCGTCAACAAACTCGATAACGCTTACATTCGCCCATT
GGCGTATGTGGGTAACGTGGGTCTTGGCGTGTGCCCGCCAGTCGGCACTGAAATGGATCTGATGATTGCCGCTTTCCCTT
GGGGGGCTTACCTCGGTGAAGAAGCGCTAGAAAATGGCGTGGATGCCATGGTTTCAAGCTGGCATCGTGCGGCACCGAAC
ACCATTCCAACCGCGGCAAAAGCTGGCGGTAATTACCTATCTTCTCTACTGGTTGGTGGCGAAGCACGTCGCCACGGTTA
CGCGGAAGGCATTGCGCTGAGTGTGGATGGTTATCTCTCTGAAGGCGCAGGCGAAAACATTTTCGTGGTGAAAAACGGCG
TGATCCTGACCCCACCGACCACCAGCTCTATCCTGCCGGGTATCACCCGTGATTCGATCATGACGCTGGCACGCGACATG
GGTTATGAAGTGCGCGAAGCCAACATCTCCCGTGAAGCGCTCTATCTGGCCGATGAAATTTTCATGACAGGTACCGCCGC
CGAAGTGGTTCCCGTGCGTAGCGTGGATAAGATTACCGTAGGCGCAGGCAAGCGCGGCCCCATCACCAAGGTAGTACAAG
ATGCTTACTTTGGTCTGTTCAAAGGCACCACAGAAGATAAGTGGGGCTGGTTGGATTACGTTTACCCAGCAGCGAAGTAA

Upstream 100 bases:

>100_bases
CCCTGACCAATCAAATTGAAAAACTGTGGGATGTGCGTACTGTGGAAGTCACAGCGATCAGAAATGACGAGCTGCCCAAT
AACAACTTACAACAAAAGAT

Downstream 100 bases:

>100_bases
GGAGTTTCCATGCCTAAGTACCGTTCCGCCACCACCACACACGGACGTAACATGGCGGGTGCGCGCGCATTATGGCGCGC
CACCGGCGTCAAAGAGGAAG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MRIRKAIMATKTADYIWFNGEMIPWANATVHVLTHAMHYGTSVFEGVRCYNTPKGPIIFRHREHAQRLLDSAKIYRFPIP
YSIETIMEATRETLRVNKLDNAYIRPLAYVGNVGLGVCPPVGTEMDLMIAAFPWGAYLGEEALENGVDAMVSSWHRAAPN
TIPTAAKAGGNYLSSLLVGGEARRHGYAEGIALSVDGYLSEGAGENIFVVKNGVILTPPTTSSILPGITRDSIMTLARDM
GYEVREANISREALYLADEIFMTGTAAEVVPVRSVDKITVGAGKRGPITKVVQDAYFGLFKGTTEDKWGWLDYVYPAAK

Sequences:

>Translated_319_residues
MRIRKAIMATKTADYIWFNGEMIPWANATVHVLTHAMHYGTSVFEGVRCYNTPKGPIIFRHREHAQRLLDSAKIYRFPIP
YSIETIMEATRETLRVNKLDNAYIRPLAYVGNVGLGVCPPVGTEMDLMIAAFPWGAYLGEEALENGVDAMVSSWHRAAPN
TIPTAAKAGGNYLSSLLVGGEARRHGYAEGIALSVDGYLSEGAGENIFVVKNGVILTPPTTSSILPGITRDSIMTLARDM
GYEVREANISREALYLADEIFMTGTAAEVVPVRSVDKITVGAGKRGPITKVVQDAYFGLFKGTTEDKWGWLDYVYPAAK
>Mature_319_residues
MRIRKAIMATKTADYIWFNGEMIPWANATVHVLTHAMHYGTSVFEGVRCYNTPKGPIIFRHREHAQRLLDSAKIYRFPIP
YSIETIMEATRETLRVNKLDNAYIRPLAYVGNVGLGVCPPVGTEMDLMIAAFPWGAYLGEEALENGVDAMVSSWHRAAPN
TIPTAAKAGGNYLSSLLVGGEARRHGYAEGIALSVDGYLSEGAGENIFVVKNGVILTPPTTSSILPGITRDSIMTLARDM
GYEVREANISREALYLADEIFMTGTAAEVVPVRSVDKITVGAGKRGPITKVVQDAYFGLFKGTTEDKWGWLDYVYPAAK

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=294, Percent_Identity=27.891156462585, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI296010904, Length=291, Percent_Identity=27.1477663230241, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI38176287, Length=291, Percent_Identity=27.1477663230241, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI296010906, Length=291, Percent_Identity=27.1477663230241, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI258614015, Length=259, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI48994963, Length=307, Percent_Identity=71.6612377850163, Blast_Score=481, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17565728, Length=283, Percent_Identity=29.6819787985866, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17568601, Length=284, Percent_Identity=28.8732394366197, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322002, Length=287, Percent_Identity=27.5261324041812, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322608, Length=335, Percent_Identity=26.2686567164179, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34984; Mature: 34984

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIRKAIMATKTADYIWFNGEMIPWANATVHVLTHAMHYGTSVFEGVRCYNTPKGPIIFR
CCHHHHHHHHCCCCEEEECCEECCCCCCHHHHHHHHHHHCHHHHCCCCCCCCCCCCEEEE
HREHAQRLLDSAKIYRFPIPYSIETIMEATRETLRVNKLDNAYIRPLAYVGNVGLGVCPP
CHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
VGTEMDLMIAAFPWGAYLGEEALENGVDAMVSSWHRAAPNTIPTAAKAGGNYLSSLLVGG
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHCC
EARRHGYAEGIALSVDGYLSEGAGENIFVVKNGVILTPPTTSSILPGITRDSIMTLARDM
CHHHCCCCCCEEEEECCCCCCCCCCEEEEEECCEEECCCCCHHHCCCCCHHHHHHHHHHC
GYEVREANISREALYLADEIFMTGTAAEVVPVRSVDKITVGAGKRGPITKVVQDAYFGLF
CCHHHHCCCCHHHHHHHHHHHCCCCCHHEEECCCCCEEEECCCCCCCHHHHHHHHHHHHH
KGTTEDKWGWLDYVYPAAK
CCCCCCCCCCEEEECCCCC
>Mature Secondary Structure
MRIRKAIMATKTADYIWFNGEMIPWANATVHVLTHAMHYGTSVFEGVRCYNTPKGPIIFR
CCHHHHHHHHCCCCEEEECCEECCCCCCHHHHHHHHHHHCHHHHCCCCCCCCCCCCEEEE
HREHAQRLLDSAKIYRFPIPYSIETIMEATRETLRVNKLDNAYIRPLAYVGNVGLGVCPP
CHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
VGTEMDLMIAAFPWGAYLGEEALENGVDAMVSSWHRAAPNTIPTAAKAGGNYLSSLLVGG
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHCC
EARRHGYAEGIALSVDGYLSEGAGENIFVVKNGVILTPPTTSSILPGITRDSIMTLARDM
CHHHCCCCCCEEEEECCCCCCCCCCEEEEEECCEEECCCCCHHHCCCCCHHHHHHHHHHC
GYEVREANISREALYLADEIFMTGTAAEVVPVRSVDKITVGAGKRGPITKVVQDAYFGLF
CCHHHHCCCCHHHHHHHHHHHCCCCCHHEEECCCCCEEEECCCCCCCHHHHHHHHHHHHH
KGTTEDKWGWLDYVYPAAK
CCCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]