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The map label for this gene is gldA [H]

Identifier: 226949296

GI number: 226949296

Start: 2282265

End: 2283356

Strand: Direct

Name: gldA [H]

Synonym: CLM_2215

Alternate gene names: 226949296

Gene position: 2282265-2283356 (Clockwise)

Preceding gene: 226949294

Following gene: 226949302

Centisome position: 54.92

GC content: 33.33

Gene sequence:

>1092_bases
ATGAGTAAAATAATAATTTCTCCAAGTAAATATGTTCAAGGGAAAAGAGAACTATCCAAATTTGGAGTTCACGCTAGATC
CTTAGGTAAAAAATTTTTAATAATAGCTAGTCCTAATGGGATAAAAAGAACTAAAACTACTTTAGAAAAAAGTTGTAAAA
ATTTTAATATACAATTAATATTTGAGCCATTTAATGGAGAATGCTGCAAAGAAGAAATAGATAGATTGTGTTCTTATGTA
GAATCTAATAACTGTAATGTAGTAGTTGGTATTGGGGGAGGTAAAATTTTTGATACAGCAAAAGCTGTAGCTTATTATAA
AAATACTCCGGTAGTTATAGCCCCAACTGTAGCTTCCACTGATGCGCCTTGTAGTGCTCTTTCGATAATATACACAAAGG
AAGGAACTTTCTCAGAGTACCTATTGCTTCCTAAAAATCCTGAATTAGTATTAGTGGACACTTCTATGGTAGCTAAGGCT
CCTGCAAGACTTTTAGCGGCAGGTATGGGAAATGCTTTAGCAACTTATTTTGAAGCTAGAGCTTGTAAAAATTCAAATTC
AGATACTTTAGCTGGTGGTAAAGGCACTAATAGTGCTTTTGCATTAGCTAAACTTTGCTATGAAACCCTTTTAAGAGATG
GACTTAAGGCAAAACTTGCTGTAGAAAATAAAGTTTCCACTAAAGCTGTAGAAAATATCATCGAAGCTAATACTTTCTTA
AGTGGAATTGGATTTGAAAGCTGTGGTTTAGCAGCAGCCCATGCCATCCATAATGGATTTACAGTATTAGAACAATGTCA
TCATTTGTACCATGGTGAAAAGGTTGCTTTTGGAACTTTAGTACAACTAGTTTTAGAGAACAGTCCACTAGAAGAAATTG
AAGAAGTTATAGAATTCTGTTTAGATGTGAACCTTCCAATAACTCTAGAAGATATGGGAATTAAGGAAATAAAAGAAGAA
GACATTATGAATGTAGCTAAAGCTACCTGCGCTGAAGGAGAAAGTATTTTCAATATGCCTTTTAAAGTAACTTACGAGGA
TGTTTATGCTGCTATTCTTACTGCAGATAATTTAGCAGATTTGTATAAATAG

Upstream 100 bases:

>100_bases
AACTGAAAATTTAAATATTTTAACAATAAATTGATAAAAATTTAACTATTTTCTGATATAATAAATATAGAACTAAATAT
ACTTTTTAGGAGGAAAGAAT

Downstream 100 bases:

>100_bases
TATATAAACGAATTATTTTTCAACATTTTTGTTAATTATTTAACCTCTTTAGAAAGGGTTCATTTATTTATAGTTTTATT
ATTATTGAATAAATGGAACT

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MSKIIISPSKYVQGKRELSKFGVHARSLGKKFLIIASPNGIKRTKTTLEKSCKNFNIQLIFEPFNGECCKEEIDRLCSYV
ESNNCNVVVGIGGGKIFDTAKAVAYYKNTPVVIAPTVASTDAPCSALSIIYTKEGTFSEYLLLPKNPELVLVDTSMVAKA
PARLLAAGMGNALATYFEARACKNSNSDTLAGGKGTNSAFALAKLCYETLLRDGLKAKLAVENKVSTKAVENIIEANTFL
SGIGFESCGLAAAHAIHNGFTVLEQCHHLYHGEKVAFGTLVQLVLENSPLEEIEEVIEFCLDVNLPITLEDMGIKEIKEE
DIMNVAKATCAEGESIFNMPFKVTYEDVYAAILTADNLADLYK

Sequences:

>Translated_363_residues
MSKIIISPSKYVQGKRELSKFGVHARSLGKKFLIIASPNGIKRTKTTLEKSCKNFNIQLIFEPFNGECCKEEIDRLCSYV
ESNNCNVVVGIGGGKIFDTAKAVAYYKNTPVVIAPTVASTDAPCSALSIIYTKEGTFSEYLLLPKNPELVLVDTSMVAKA
PARLLAAGMGNALATYFEARACKNSNSDTLAGGKGTNSAFALAKLCYETLLRDGLKAKLAVENKVSTKAVENIIEANTFL
SGIGFESCGLAAAHAIHNGFTVLEQCHHLYHGEKVAFGTLVQLVLENSPLEEIEEVIEFCLDVNLPITLEDMGIKEIKEE
DIMNVAKATCAEGESIFNMPFKVTYEDVYAAILTADNLADLYK
>Mature_362_residues
SKIIISPSKYVQGKRELSKFGVHARSLGKKFLIIASPNGIKRTKTTLEKSCKNFNIQLIFEPFNGECCKEEIDRLCSYVE
SNNCNVVVGIGGGKIFDTAKAVAYYKNTPVVIAPTVASTDAPCSALSIIYTKEGTFSEYLLLPKNPELVLVDTSMVAKAP
ARLLAAGMGNALATYFEARACKNSNSDTLAGGKGTNSAFALAKLCYETLLRDGLKAKLAVENKVSTKAVENIIEANTFLS
GIGFESCGLAAAHAIHNGFTVLEQCHHLYHGEKVAFGTLVQLVLENSPLEEIEEVIEFCLDVNLPITLEDMGIKEIKEED
IMNVAKATCAEGESIFNMPFKVTYEDVYAAILTADNLADLYK

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. Exhibits a rather broad substrate specificity since it can also oxidize 1,2-prop

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=362, Percent_Identity=55.524861878453, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1786815, Length=295, Percent_Identity=31.5254237288136, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 39394; Mature: 39263

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIIISPSKYVQGKRELSKFGVHARSLGKKFLIIASPNGIKRTKTTLEKSCKNFNIQLI
CCCEEECCHHHHHHHHHHHHHCHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEE
FEPFNGECCKEEIDRLCSYVESNNCNVVVGIGGGKIFDTAKAVAYYKNTPVVIAPTVAST
EECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHEEECCCCEEEEECCCCC
DAPCSALSIIYTKEGTFSEYLLLPKNPELVLVDTSMVAKAPARLLAAGMGNALATYFEAR
CCCCCEEEEEEECCCCCCEEEEECCCCCEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHH
ACKNSNSDTLAGGKGTNSAFALAKLCYETLLRDGLKAKLAVENKVSTKAVENIIEANTFL
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHH
SGIGFESCGLAAAHAIHNGFTVLEQCHHLYHGEKVAFGTLVQLVLENSPLEEIEEVIEFC
HCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
LDVNLPITLEDMGIKEIKEEDIMNVAKATCAEGESIFNMPFKVTYEDVYAAILTADNLAD
HCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEHHHHHHHHHHHHHHHH
LYK
HCC
>Mature Secondary Structure 
SKIIISPSKYVQGKRELSKFGVHARSLGKKFLIIASPNGIKRTKTTLEKSCKNFNIQLI
CCEEECCHHHHHHHHHHHHHCHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEE
FEPFNGECCKEEIDRLCSYVESNNCNVVVGIGGGKIFDTAKAVAYYKNTPVVIAPTVAST
EECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHEEECCCCEEEEECCCCC
DAPCSALSIIYTKEGTFSEYLLLPKNPELVLVDTSMVAKAPARLLAAGMGNALATYFEAR
CCCCCEEEEEEECCCCCCEEEEECCCCCEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHH
ACKNSNSDTLAGGKGTNSAFALAKLCYETLLRDGLKAKLAVENKVSTKAVENIIEANTFL
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHH
SGIGFESCGLAAAHAIHNGFTVLEQCHHLYHGEKVAFGTLVQLVLENSPLEEIEEVIEFC
HCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
LDVNLPITLEDMGIKEIKEEDIMNVAKATCAEGESIFNMPFKVTYEDVYAAILTADNLAD
HCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEHHHHHHHHHHHHHHHH
LYK
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA