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The map label for this gene is moaA

Identifier: 226949238

GI number: 226949238

Start: 2212684

End: 2213643

Strand: Reverse

Name: moaA

Synonym: CLM_2157

Alternate gene names: 226949238

Gene position: 2213643-2212684 (Counterclockwise)

Preceding gene: 226949239

Following gene: 226949237

Centisome position: 53.27

GC content: 28.75

Gene sequence:

>960_bases
ATGTTAGATAAGCATGGAAGGAAAATTAACTATTTAAGAGTATCTGTAACAGATAGATGCAATTTAAGATGTGTTTATTG
TATGCCACCTGAAGGTATTGTAAAAAAAGAACATGATAATATAATGAGATATGAAGAGATATTTAAGGTGGTTAAGTCAG
CTAGTTTATTAGGAGTAAATAAAATTAGATTTACAGGTGGAGAACCTCTTATACTTAAAGATATAGATAAATTAATTTAT
AATACATCAAAAATAAATTCCATTAAAGACATTGCCATGACTACCAATGCTATACTTTTAGAAGATATGGTAGAGGAATT
AAAAAAGGCTGGTCTTAAAAGAGTTAATATAAGTCTTGATTCCTTAAAGGAAGACAGGTTTAAGAGCATTACAAGAGGTG
GGGATATTAATAAAGTTTTTAAAAGCATTGAAAAATCTTTATCCATTGGTATGAAACCAATAAAAATTAATACAGTAATA
ATGAAGGGCATAAATGATGATGAAATAGATGATTTTATGAATTTAACAAAGAAATATCCTATATCTGTAAGATTTATAGA
GCTTATGCCTATAGGAGAAGGTAGAAAATTATACAAAGATGGTTATATAAGTTCAGAAGAAATAATATCCAAGCATAGTG
ACCTTATACCTGTGGAAACTGAGAAAAGTAGCACTGCTCTATTATATAAATTTAAAGAGTCTAAAGAAAATATTGGATTC
ATAAGCCCTATGAGTTGTAAATTTTGCAGCGGCTGTAATAGAGTAAGATTAACTTCAGAGGGCACATTGAAACCTTGCCT
TCATTCTGAAAAGGAAGTAAATCTTAAAAACTATGTAGGAAATAACCAAGCACTTTTATCTAAGATAAATGAAACTATTT
ATAACAAACCCTTAGAGCATCATATGATAGAGGAAAAAGAAAGTAAAAGCAAAAAAATGATGTATCAGATTGGAGGTTAG

Upstream 100 bases:

>100_bases
GGTTATGACTATAGTGAAGCAGGTAATATTGTTTTTATATAAGAAATGATAGATATGTTTCATATACTAAAGAGTAAGCT
TTATATAAAGGAGGGGTTTG

Downstream 100 bases:

>100_bases
CTTATGGAATTAACACATATAAATAAGCAAGGAAGAGCAAAAATGGTGGATGTAGGCAATAAAGGAGATACAGAAAGGGA
AGCTATTGCCTATGGATATG

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MLDKHGRKINYLRVSVTDRCNLRCVYCMPPEGIVKKEHDNIMRYEEIFKVVKSASLLGVNKIRFTGGEPLILKDIDKLIY
NTSKINSIKDIAMTTNAILLEDMVEELKKAGLKRVNISLDSLKEDRFKSITRGGDINKVFKSIEKSLSIGMKPIKINTVI
MKGINDDEIDDFMNLTKKYPISVRFIELMPIGEGRKLYKDGYISSEEIISKHSDLIPVETEKSSTALLYKFKESKENIGF
ISPMSCKFCSGCNRVRLTSEGTLKPCLHSEKEVNLKNYVGNNQALLSKINETIYNKPLEHHMIEEKESKSKKMMYQIGG

Sequences:

>Translated_319_residues
MLDKHGRKINYLRVSVTDRCNLRCVYCMPPEGIVKKEHDNIMRYEEIFKVVKSASLLGVNKIRFTGGEPLILKDIDKLIY
NTSKINSIKDIAMTTNAILLEDMVEELKKAGLKRVNISLDSLKEDRFKSITRGGDINKVFKSIEKSLSIGMKPIKINTVI
MKGINDDEIDDFMNLTKKYPISVRFIELMPIGEGRKLYKDGYISSEEIISKHSDLIPVETEKSSTALLYKFKESKENIGF
ISPMSCKFCSGCNRVRLTSEGTLKPCLHSEKEVNLKNYVGNNQALLSKINETIYNKPLEHHMIEEKESKSKKMMYQIGG
>Mature_319_residues
MLDKHGRKINYLRVSVTDRCNLRCVYCMPPEGIVKKEHDNIMRYEEIFKVVKSASLLGVNKIRFTGGEPLILKDIDKLIY
NTSKINSIKDIAMTTNAILLEDMVEELKKAGLKRVNISLDSLKEDRFKSITRGGDINKVFKSIEKSLSIGMKPIKINTVI
MKGINDDEIDDFMNLTKKYPISVRFIELMPIGEGRKLYKDGYISSEEIISKHSDLIPVETEKSSTALLYKFKESKENIGF
ISPMSCKFCSGCNRVRLTSEGTLKPCLHSEKEVNLKNYVGNNQALLSKINETIYNKPLEHHMIEEKESKSKKMMYQIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=326, Percent_Identity=37.7300613496933, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI22027536, Length=326, Percent_Identity=37.7300613496933, Blast_Score=232, Evalue=4e-61,
Organism=Escherichia coli, GI1786999, Length=329, Percent_Identity=33.434650455927, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI32566329, Length=328, Percent_Identity=38.4146341463415, Blast_Score=229, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17561210, Length=328, Percent_Identity=38.719512195122, Blast_Score=228, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI32566331, Length=318, Percent_Identity=38.6792452830189, Blast_Score=226, Evalue=9e-60,
Organism=Drosophila melanogaster, GI28574488, Length=327, Percent_Identity=35.1681957186544, Blast_Score=210, Evalue=8e-55,
Organism=Drosophila melanogaster, GI28574490, Length=281, Percent_Identity=38.0782918149466, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_CLOB1 (A7FUZ6)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384199.1
- ProteinModelPortal:   A7FUZ6
- SMR:   A7FUZ6
- STRING:   A7FUZ6
- GeneID:   5397415
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_1878
- eggNOG:   COG2896
- HOGENOM:   HBG529885
- OMA:   IPVETDK
- ProtClustDB:   CLSK882997
- BioCyc:   CBOT441770:CLB_1878-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 36402; Mature: 36402

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 13-13 BINDING 26-26 BINDING 63-63 BINDING 67-67 BINDING 94-94 BINDING 118-118 BINDING 155-155 BINDING 189-189

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDKHGRKINYLRVSVTDRCNLRCVYCMPPEGIVKKEHDNIMRYEEIFKVVKSASLLGVN
CCCCCCCEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCE
KIRFTGGEPLILKDIDKLIYNTSKINSIKDIAMTTNAILLEDMVEELKKAGLKRVNISLD
EEEECCCCEEHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHH
SLKEDRFKSITRGGDINKVFKSIEKSLSIGMKPIKINTVIMKGINDDEIDDFMNLTKKYP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCC
ISVRFIELMPIGEGRKLYKDGYISSEEIISKHSDLIPVETEKSSTALLYKFKESKENIGF
CEEEEEEEEECCCCCHHHHCCCCCHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCCCC
ISPMSCKFCSGCNRVRLTSEGTLKPCLHSEKEVNLKNYVGNNQALLSKINETIYNKPLEH
CCCCCCHHHCCCCEEEECCCCCCCHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCHHH
HMIEEKESKSKKMMYQIGG
HHHHHHHHHHHHHEEECCC
>Mature Secondary Structure
MLDKHGRKINYLRVSVTDRCNLRCVYCMPPEGIVKKEHDNIMRYEEIFKVVKSASLLGVN
CCCCCCCEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCE
KIRFTGGEPLILKDIDKLIYNTSKINSIKDIAMTTNAILLEDMVEELKKAGLKRVNISLD
EEEECCCCEEHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHH
SLKEDRFKSITRGGDINKVFKSIEKSLSIGMKPIKINTVIMKGINDDEIDDFMNLTKKYP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCC
ISVRFIELMPIGEGRKLYKDGYISSEEIISKHSDLIPVETEKSSTALLYKFKESKENIGF
CEEEEEEEEECCCCCHHHHCCCCCHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCCCC
ISPMSCKFCSGCNRVRLTSEGTLKPCLHSEKEVNLKNYVGNNQALLSKINETIYNKPLEH
CCCCCCHHHCCCCEEEECCCCCCCHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCHHH
HMIEEKESKSKKMMYQIGG
HHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA