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The map label for this gene is tyrS [H]

Identifier: 226948471

GI number: 226948471

Start: 1422265

End: 1423467

Strand: Direct

Name: tyrS [H]

Synonym: CLM_1358

Alternate gene names: 226948471

Gene position: 1422265-1423467 (Clockwise)

Preceding gene: 226948470

Following gene: 226948472

Centisome position: 34.23

GC content: 29.09

Gene sequence:

>1203_bases
ATGAAAAGTATAGAAGAACAAATAAAAATAATATCTAAAGGTGTAGACGAAATTATAAATGTAGAGGATTTAAAAAATAA
ACTAGAAAGATCACAAAAAACAGGAAAACCTCTGACAGTTAAGTTAGGATTAGACCCTACAGCACCTGACATTCATATAG
GTCATTCAGTAGTTTTAAGAAAAATAAGACAACTGCAAGACTTAGGACATAGAGCAGTTATAATAATAGGAGACTTTACA
GGAAGAATAGGAGATCCAACAGGAAAGTCTAAAACTAGAAAACCATTGACACAAGAAGAAGTTATGTTTAATGCTAAAAC
CTATGAAGAACAAATATTTAAAATAATAGATAAAGATAAGACTGATTTAAAATTTAATAGCGAATGGTTAGGAAAATTAT
CTTTAAGAGATACTATAGAGTTAACTTCAAAATATACAGTGGCTAGAATGCTTGAAAGAGAAGATTTTAAAAAGAGATTT
GAAAGTCACTCTAGTATAGGAATACACGAATTCTTTTACCCATTATTACAAGCCTTTGATTCTGTTAGTCTAAATGCGGA
TATAGAATTAGGAGGAACAGACCAAAGATTTAATCTTCTTATGGGAAGAACTATACAAAAGGATTTTGATCAAGAAAGCC
AAGTAGCTATTCTTATGCCATTATTAGAAGGAACAGATGGAAAAGACAAAATGAGCAAAAGTTTAGGAAACTATATAGGA
ATAAATGAAGATGCCAATGATATATACGGAAAGGTTATGTCTATACCAGATGGAATGATAGTTAAACACTATGAATTAGC
TACAGACATACATCCAGATGAAGTAGCAAAAATAAAAACAAGATTAGAAGAGGAAAATGTTAATCCAAGAGATATTAAAA
TGGAACTTGCTAAAGAAATAGTAAGACTATATCATGGAGAAGAAGCATCTGTAAAAGCAGAAGTAAACTTCAAAAATATA
TTCCAACAAGGTAATATACCAGAAGATATAGATACAATAAAGGTAAATTTAACTGAAACAAATATAGTAGACTTGATAGT
TAAAGCAAAATTTGCTCCTAGTAAAAACGAAGCTAGAAGATTAGTGAAACAAGGAGCAGTAAAAATAAATGGAGAAAAAG
TAGACGAAGAATGTAAAGTAAAAAATGAAGATATACTTCAAGTAGGAAAGAAGAAGTTTATAAAAATATTAAGTCAAAAT
TAA

Upstream 100 bases:

>100_bases
AAGCGCTTAGAAAATTAATATTTAAATCTAAAATAATAAAATAATTACCATATGCATATGGCCTTACAAAAAATATTATA
ATTTTAGGGAGGCTATAAAA

Downstream 100 bases:

>100_bases
TTTTATAAAAGTGGATTACTTATATTGTGAGTATAGATTAAAAAATTAATTATAAAAAACATTATCATTTAATTTAAAAT
GGTAATGTTTTTTTTATGAG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase 2; TyrRS 2 [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKSIEEQIKIISKGVDEIINVEDLKNKLERSQKTGKPLTVKLGLDPTAPDIHIGHSVVLRKIRQLQDLGHRAVIIIGDFT
GRIGDPTGKSKTRKPLTQEEVMFNAKTYEEQIFKIIDKDKTDLKFNSEWLGKLSLRDTIELTSKYTVARMLEREDFKKRF
ESHSSIGIHEFFYPLLQAFDSVSLNADIELGGTDQRFNLLMGRTIQKDFDQESQVAILMPLLEGTDGKDKMSKSLGNYIG
INEDANDIYGKVMSIPDGMIVKHYELATDIHPDEVAKIKTRLEEENVNPRDIKMELAKEIVRLYHGEEASVKAEVNFKNI
FQQGNIPEDIDTIKVNLTETNIVDLIVKAKFAPSKNEARRLVKQGAVKINGEKVDEECKVKNEDILQVGKKKFIKILSQN

Sequences:

>Translated_400_residues
MKSIEEQIKIISKGVDEIINVEDLKNKLERSQKTGKPLTVKLGLDPTAPDIHIGHSVVLRKIRQLQDLGHRAVIIIGDFT
GRIGDPTGKSKTRKPLTQEEVMFNAKTYEEQIFKIIDKDKTDLKFNSEWLGKLSLRDTIELTSKYTVARMLEREDFKKRF
ESHSSIGIHEFFYPLLQAFDSVSLNADIELGGTDQRFNLLMGRTIQKDFDQESQVAILMPLLEGTDGKDKMSKSLGNYIG
INEDANDIYGKVMSIPDGMIVKHYELATDIHPDEVAKIKTRLEEENVNPRDIKMELAKEIVRLYHGEEASVKAEVNFKNI
FQQGNIPEDIDTIKVNLTETNIVDLIVKAKFAPSKNEARRLVKQGAVKINGEKVDEECKVKNEDILQVGKKKFIKILSQN
>Mature_400_residues
MKSIEEQIKIISKGVDEIINVEDLKNKLERSQKTGKPLTVKLGLDPTAPDIHIGHSVVLRKIRQLQDLGHRAVIIIGDFT
GRIGDPTGKSKTRKPLTQEEVMFNAKTYEEQIFKIIDKDKTDLKFNSEWLGKLSLRDTIELTSKYTVARMLEREDFKKRF
ESHSSIGIHEFFYPLLQAFDSVSLNADIELGGTDQRFNLLMGRTIQKDFDQESQVAILMPLLEGTDGKDKMSKSLGNYIG
INEDANDIYGKVMSIPDGMIVKHYELATDIHPDEVAKIKTRLEEENVNPRDIKMELAKEIVRLYHGEEASVKAEVNFKNI
FQQGNIPEDIDTIKVNLTETNIVDLIVKAKFAPSKNEARRLVKQGAVKINGEKVDEECKVKNEDILQVGKKKFIKILSQN

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=410, Percent_Identity=27.3170731707317, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI1787925, Length=428, Percent_Identity=27.3364485981308, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25145802, Length=373, Percent_Identity=27.8820375335121, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25145805, Length=308, Percent_Identity=25.3246753246753, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6325160, Length=424, Percent_Identity=25.9433962264151, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI20130383, Length=412, Percent_Identity=26.4563106796117, Blast_Score=139, Evalue=5e-33,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 45449; Mature: 45449

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIEEQIKIISKGVDEIINVEDLKNKLERSQKTGKPLTVKLGLDPTAPDIHIGHSVVLR
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHH
KIRQLQDLGHRAVIIIGDFTGRIGDPTGKSKTRKPLTQEEVMFNAKTYEEQIFKIIDKDK
HHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHCCCC
TDLKFNSEWLGKLSLRDTIELTSKYTVARMLEREDFKKRFESHSSIGIHEFFYPLLQAFD
CCCEECHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
SVSLNADIELGGTDQRFNLLMGRTIQKDFDQESQVAILMPLLEGTDGKDKMSKSLGNYIG
CCCCCCEEEECCCCHHHHHHHCCHHHHHCCCCCCEEEEEEHHCCCCCHHHHHHHHCCEEC
INEDANDIYGKVMSIPDGMIVKHYELATDIHPDEVAKIKTRLEEENVNPRDIKMELAKEI
CCCCHHHHHHHHHCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VRLYHGEEASVKAEVNFKNIFQQGNIPEDIDTIKVNLTETNIVDLIVKAKFAPSKNEARR
HHHHCCCCCCEEEEECHHHHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCHHHHHH
LVKQGAVKINGEKVDEECKVKNEDILQVGKKKFIKILSQN
HHHHCCEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSIEEQIKIISKGVDEIINVEDLKNKLERSQKTGKPLTVKLGLDPTAPDIHIGHSVVLR
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHH
KIRQLQDLGHRAVIIIGDFTGRIGDPTGKSKTRKPLTQEEVMFNAKTYEEQIFKIIDKDK
HHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHCCCC
TDLKFNSEWLGKLSLRDTIELTSKYTVARMLEREDFKKRFESHSSIGIHEFFYPLLQAFD
CCCEECHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
SVSLNADIELGGTDQRFNLLMGRTIQKDFDQESQVAILMPLLEGTDGKDKMSKSLGNYIG
CCCCCCEEEECCCCHHHHHHHCCHHHHHCCCCCCEEEEEEHHCCCCCHHHHHHHHCCEEC
INEDANDIYGKVMSIPDGMIVKHYELATDIHPDEVAKIKTRLEEENVNPRDIKMELAKEI
CCCCHHHHHHHHHCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VRLYHGEEASVKAEVNFKNIFQQGNIPEDIDTIKVNLTETNIVDLIVKAKFAPSKNEARR
HHHHCCCCCCEEEEECHHHHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCHHHHHH
LVKQGAVKINGEKVDEECKVKNEDILQVGKKKFIKILSQN
HHHHCCEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]