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The map label for this gene is pyrG

Identifier: 226225106

GI number: 226225106

Start: 2609280

End: 2610878

Strand: Reverse

Name: pyrG

Synonym: Lm4b_02527

Alternate gene names: 226225106

Gene position: 2610878-2609280 (Counterclockwise)

Preceding gene: 226225107

Following gene: 226225104

Centisome position: 89.64

GC content: 39.09

Gene sequence:

>1599_bases
ATGACAAAGTATATTTTCGTTACAGGTGGCGTAGTTTCGTCAATCGGAAAAGGAATCACAGCAGCATCACTCGGACGTTT
GCTGAAAAATCGTGGACTTAGTGTAACCATTCAAAAATTTGATCCATACATCAACGTAGATCCAGGAACAATGAGTCCAT
ACCAACACGGGGAAGTTTATGTGACAGATGACGGCGCGGAAACGGACCTAGACCTTGGCCATTACGAACGTTTTATCGAT
ATTAACTTAAATAAATACAGCAACGTGACAACTGGTAAAGTTTACTCGGAAGTTATTAAAAAAGAACGTCGCGGGGATTA
CTTAGGTGGAACAGTGCAAGTTATTCCACACATTACAAACGAATTAAAAGACCGTGTTTTCCGTGCAGCGCGCATGACCA
ATTCAGATATTATCATCACTGAAATTGGTGGAACAGTTGGGGATATCGAATCTTTACCATTCTTAGAAGCAATTCGCCAA
ATTAAAAGTGATGTTGGTGCAGAAAACGTACTTTATATCCACACAACTTTAATTCCTTACATCAAAGCAGCTGGCGAAAT
GAAAACTAAACCAACACAACATAGTGTAAAAGAGCTTCGTAGCTTAGGAATTCAACCAAACATTATCGTTGTTCGCACAG
AGCAGCCAGTTTCACAAGAAATGAAAGATAAGATTGCGCTATTCTGTGATATTAAAGCTTCCGAGGTTATTGAATCTCGC
GATGAAGAAACACTTTATAACGTACCACTTTCTCTACAAAAACAAAAAATGGATGATATCGTCCTTGAGCACTTACAATT
AGAAGCACCACAAGCGGAAATGACGGATTGGAAAAACTTAGTACATCGTGTGAAAAATCTTTCCAAAAAAGTTCGTATTG
GCCTAGTTGGTAAATATGTTTCCTTGCAAGATGCTTACCTTTCTGTAGCAGAAGCACTTCGTCATGCAGGATATGATCAT
GATGCAGAAATCGAAATTGACTGGATTGATTCCGAAAAAGTAACAAAAGAAAATGTTGCTGAAATCATGAAAGACGTTGA
TGGTATCTTAGTTCCTGGTGGTTTCGGCGACCGTGCCATTGAAGGTAAAATTGCTGCTATTGAATATGCACGCGTAAACA
AAGTGCCTTATTTTGGTATTTGTTTAGGAATGCAACTAGCAACTGTTGAATTTGCCCGTAATGTTCTTGGTCTTGAAGGA
GCTCATTCTGCAGAAATCGAACCAGAAACAAAACATAACATCATTGATTTATTACCAGAACAAAAGAACATCGAAAACAT
GGGCGGAACACTTCGTTTAGGACTTTATCCAGCGCGTATTAAGCAAGGTACTAAAGCCGAGGCCGCTTATGGTACAACTC
TTGTTGAAGAGCGTCACCGTCATCGTTATGAGTTTAATAACGAATATAGAGAGCAGATGGAAGAAGCTGGTATGATTGTT
TCTGCAACAAGTCCAGATGGTCGTTTAGTTGAAGTAGTTGAATTAGCTGATCATCCTTGGTTTGTCGCTTGTCAATATCA
CCCAGAATTCATTTCTCGTCCAAATCGTCCGCAAAGCTTGTTTAAAGATTTTGTTGGCGCTGCACTTAATAATAAATAA

Upstream 100 bases:

>100_bases
ATAATCACAATATTGTTTTATCTGGCAATTTCCCACCCCAGCAGGAAAATCGTCAGACCCTGCAAGTTTTTCATTTCAGG
AAGGAGTAGATTTTTTTAAC

Downstream 100 bases:

>100_bases
GGTAATAATAAAAATCTCCCAGCCATAATTGGCTGGGAGATTTTTATTATTATTATTTAGTAGCACTAAGGTTCGAAGCT
TTTGTCCAACCAATAAAGGT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MTKYIFVTGGVVSSIGKGITAASLGRLLKNRGLSVTIQKFDPYINVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFID
INLNKYSNVTTGKVYSEVIKKERRGDYLGGTVQVIPHITNELKDRVFRAARMTNSDIIITEIGGTVGDIESLPFLEAIRQ
IKSDVGAENVLYIHTTLIPYIKAAGEMKTKPTQHSVKELRSLGIQPNIIVVRTEQPVSQEMKDKIALFCDIKASEVIESR
DEETLYNVPLSLQKQKMDDIVLEHLQLEAPQAEMTDWKNLVHRVKNLSKKVRIGLVGKYVSLQDAYLSVAEALRHAGYDH
DAEIEIDWIDSEKVTKENVAEIMKDVDGILVPGGFGDRAIEGKIAAIEYARVNKVPYFGICLGMQLATVEFARNVLGLEG
AHSAEIEPETKHNIIDLLPEQKNIENMGGTLRLGLYPARIKQGTKAEAAYGTTLVEERHRHRYEFNNEYREQMEEAGMIV
SATSPDGRLVEVVELADHPWFVACQYHPEFISRPNRPQSLFKDFVGAALNNK

Sequences:

>Translated_532_residues
MTKYIFVTGGVVSSIGKGITAASLGRLLKNRGLSVTIQKFDPYINVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFID
INLNKYSNVTTGKVYSEVIKKERRGDYLGGTVQVIPHITNELKDRVFRAARMTNSDIIITEIGGTVGDIESLPFLEAIRQ
IKSDVGAENVLYIHTTLIPYIKAAGEMKTKPTQHSVKELRSLGIQPNIIVVRTEQPVSQEMKDKIALFCDIKASEVIESR
DEETLYNVPLSLQKQKMDDIVLEHLQLEAPQAEMTDWKNLVHRVKNLSKKVRIGLVGKYVSLQDAYLSVAEALRHAGYDH
DAEIEIDWIDSEKVTKENVAEIMKDVDGILVPGGFGDRAIEGKIAAIEYARVNKVPYFGICLGMQLATVEFARNVLGLEG
AHSAEIEPETKHNIIDLLPEQKNIENMGGTLRLGLYPARIKQGTKAEAAYGTTLVEERHRHRYEFNNEYREQMEEAGMIV
SATSPDGRLVEVVELADHPWFVACQYHPEFISRPNRPQSLFKDFVGAALNNK
>Mature_531_residues
TKYIFVTGGVVSSIGKGITAASLGRLLKNRGLSVTIQKFDPYINVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDI
NLNKYSNVTTGKVYSEVIKKERRGDYLGGTVQVIPHITNELKDRVFRAARMTNSDIIITEIGGTVGDIESLPFLEAIRQI
KSDVGAENVLYIHTTLIPYIKAAGEMKTKPTQHSVKELRSLGIQPNIIVVRTEQPVSQEMKDKIALFCDIKASEVIESRD
EETLYNVPLSLQKQKMDDIVLEHLQLEAPQAEMTDWKNLVHRVKNLSKKVRIGLVGKYVSLQDAYLSVAEALRHAGYDHD
AEIEIDWIDSEKVTKENVAEIMKDVDGILVPGGFGDRAIEGKIAAIEYARVNKVPYFGICLGMQLATVEFARNVLGLEGA
HSAEIEPETKHNIIDLLPEQKNIENMGGTLRLGLYPARIKQGTKAEAAYGTTLVEERHRHRYEFNNEYREQMEEAGMIVS
ATSPDGRLVEVVELADHPWFVACQYHPEFISRPNRPQSLFKDFVGAALNNK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=548, Percent_Identity=46.5328467153285, Blast_Score=498, Evalue=1e-141,
Organism=Homo sapiens, GI28559085, Length=550, Percent_Identity=45.4545454545455, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI28559083, Length=550, Percent_Identity=45.4545454545455, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI221316689, Length=550, Percent_Identity=45.4545454545455, Blast_Score=488, Evalue=1e-138,
Organism=Escherichia coli, GI1789142, Length=537, Percent_Identity=51.9553072625698, Blast_Score=570, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI25148299, Length=603, Percent_Identity=39.3034825870647, Blast_Score=422, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6319432, Length=558, Percent_Identity=43.3691756272401, Blast_Score=473, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6322563, Length=560, Percent_Identity=44.2857142857143, Blast_Score=472, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24664469, Length=549, Percent_Identity=45.7194899817851, Blast_Score=492, Evalue=1e-139,
Organism=Drosophila melanogaster, GI21357815, Length=494, Percent_Identity=43.5222672064777, Blast_Score=415, Evalue=1e-116,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59556; Mature: 59425

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYIFVTGGVVSSIGKGITAASLGRLLKNRGLSVTIQKFDPYINVDPGTMSPYQHGEVY
CCEEEEEECHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFIDINLNKYSNVTTGKVYSEVIKKERRGDYLGGTVQVIPHITN
EECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHEEHHHHHH
ELKDRVFRAARMTNSDIIITEIGGTVGDIESLPFLEAIRQIKSDVGAENVLYIHTTLIPY
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHH
IKAAGEMKTKPTQHSVKELRSLGIQPNIIVVRTEQPVSQEMKDKIALFCDIKASEVIESR
HHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCEEEEEECCHHHHHHCC
DEETLYNVPLSLQKQKMDDIVLEHLQLEAPQAEMTDWKNLVHRVKNLSKKVRIGLVGKYV
CCCEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECHH
SLQDAYLSVAEALRHAGYDHDAEIEIDWIDSEKVTKENVAEIMKDVDGILVPGGFGDRAI
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCCCCCCC
EGKIAAIEYARVNKVPYFGICLGMQLATVEFARNVLGLEGAHSAEIEPETKHNIIDLLPE
CCEEEEEEEHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCHHHHCCC
QKNIENMGGTLRLGLYPARIKQGTKAEAAYGTTLVEERHRHRYEFNNEYREQMEEAGMIV
CCCHHHCCCEEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEE
SATSPDGRLVEVVELADHPWFVACQYHPEFISRPNRPQSLFKDFVGAALNNK
EECCCCCCEEEEEECCCCCEEEEEEECHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKYIFVTGGVVSSIGKGITAASLGRLLKNRGLSVTIQKFDPYINVDPGTMSPYQHGEVY
CEEEEEECHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFIDINLNKYSNVTTGKVYSEVIKKERRGDYLGGTVQVIPHITN
EECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHEEHHHHHH
ELKDRVFRAARMTNSDIIITEIGGTVGDIESLPFLEAIRQIKSDVGAENVLYIHTTLIPY
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHH
IKAAGEMKTKPTQHSVKELRSLGIQPNIIVVRTEQPVSQEMKDKIALFCDIKASEVIESR
HHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCEEEEEECCHHHHHHCC
DEETLYNVPLSLQKQKMDDIVLEHLQLEAPQAEMTDWKNLVHRVKNLSKKVRIGLVGKYV
CCCEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECHH
SLQDAYLSVAEALRHAGYDHDAEIEIDWIDSEKVTKENVAEIMKDVDGILVPGGFGDRAI
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCCCCCCC
EGKIAAIEYARVNKVPYFGICLGMQLATVEFARNVLGLEGAHSAEIEPETKHNIIDLLPE
CCEEEEEEEHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCHHHHCCC
QKNIENMGGTLRLGLYPARIKQGTKAEAAYGTTLVEERHRHRYEFNNEYREQMEEAGMIV
CCCHHHCCCEEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEE
SATSPDGRLVEVVELADHPWFVACQYHPEFISRPNRPQSLFKDFVGAALNNK
EECCCCCCEEEEEECCCCCEEEEEEECHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]