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The map label for this gene is dagK [H]

Identifier: 226225104

GI number: 226225104

Start: 2605678

End: 2606598

Strand: Reverse

Name: dagK [H]

Synonym: Lm4b_02525

Alternate gene names: 226225104

Gene position: 2606598-2605678 (Counterclockwise)

Preceding gene: 226225106

Following gene: 226225103

Centisome position: 89.49

GC content: 36.92

Gene sequence:

>921_bases
TTGCAGAAGAAAGCGATGATAATATACAATCCGGCAGCGGGTAAAAATAAGTTTAGAAAATTACTTCCGGATGCAGAAAA
AATTTTAACAGAAGCAGATTTTGAAGTAACTTTAGTCCCATCAACTCCAGCACCTAAAAGTACTACATTTATTGCCAAAC
AAGCTGCAGAAGCCGGTTTTGATGTTGTAATTGCAGCAGGTGGAGATGGTACAGTAAATGAAGTTGTTAATGGGTTGATG
CAAGTTGATACCCCACCGAAACTAGGAGTGTTACCGGTTGGAACGACGAATGATTATGCGAGAGCGTTAAATTTTGCTAA
AAATCCACTCGAAGCACTTCGAATTATAGCCAAGCAAGAAACAATTCGTGTCGATATCGGTAAAGCCAATGAGACCGAAT
TTTTCATTAATAACGCAGCTGGTGGGAAAATAACCGAAATAACTTATGCTGTAAAAGAATCAATGAAATCCAAATGGGGC
AGACTTGCTTATCTGTTTAGCGGTTTAACTGTTCTTCCAAAACTATCACCAGTGTACGTCGAAATTGCATATAACGATAA
AATATTTAAAGGTGAGATTTTGCTGTTTTTTGTAAATAAGTCCAACTCGGTCGGTGGTATGGAGACATTGTGTCCTCCTG
CTGAATTAAATAGTGGAATGTTTGAGTTATTAATTTTAAAGAAAGTCTCTCCTAAAACATTATTTCAATTATTTGCTTCT
ATAAAAAAGGGTACACACTTAAGTAGTCCGCATGTAATCCATGCGCGAACAAATAAAGTTACTATAAACAGCGAAGCAGA
TTTAAATGTTAGCTATGATGGTGTATATGGAGGAAAAGCACCGTATACATTAGAGGTAATCCCAGAAGCATTAGAAGTAT
TTGCTGATACAAATCGGATTTCTTCCCGTCTTAGAGGCTAA

Upstream 100 bases:

>100_bases
CCTTCAATTTAACTAGCGTTTTCTTTCAGTCAAACAAGTTGACATCCTAAGGCCAAAATAGGAAACTAAATTATATATAT
AGGAAAGAGGGAGCGGCAAT

Downstream 100 bases:

>100_bases
AGCGTTTACACTAGAAAATTTTCTAGTTTTTATGGTAAATTCATCACAAATCTGTTATCATATGTTTGTTAGGGGAAAAT
AGCTGCCACTATTTTTCACG

Product: putative lipid kinase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MQKKAMIIYNPAAGKNKFRKLLPDAEKILTEADFEVTLVPSTPAPKSTTFIAKQAAEAGFDVVIAAGGDGTVNEVVNGLM
QVDTPPKLGVLPVGTTNDYARALNFAKNPLEALRIIAKQETIRVDIGKANETEFFINNAAGGKITEITYAVKESMKSKWG
RLAYLFSGLTVLPKLSPVYVEIAYNDKIFKGEILLFFVNKSNSVGGMETLCPPAELNSGMFELLILKKVSPKTLFQLFAS
IKKGTHLSSPHVIHARTNKVTINSEADLNVSYDGVYGGKAPYTLEVIPEALEVFADTNRISSRLRG

Sequences:

>Translated_306_residues
MQKKAMIIYNPAAGKNKFRKLLPDAEKILTEADFEVTLVPSTPAPKSTTFIAKQAAEAGFDVVIAAGGDGTVNEVVNGLM
QVDTPPKLGVLPVGTTNDYARALNFAKNPLEALRIIAKQETIRVDIGKANETEFFINNAAGGKITEITYAVKESMKSKWG
RLAYLFSGLTVLPKLSPVYVEIAYNDKIFKGEILLFFVNKSNSVGGMETLCPPAELNSGMFELLILKKVSPKTLFQLFAS
IKKGTHLSSPHVIHARTNKVTINSEADLNVSYDGVYGGKAPYTLEVIPEALEVFADTNRISSRLRG
>Mature_306_residues
MQKKAMIIYNPAAGKNKFRKLLPDAEKILTEADFEVTLVPSTPAPKSTTFIAKQAAEAGFDVVIAAGGDGTVNEVVNGLM
QVDTPPKLGVLPVGTTNDYARALNFAKNPLEALRIIAKQETIRVDIGKANETEFFINNAAGGKITEITYAVKESMKSKWG
RLAYLFSGLTVLPKLSPVYVEIAYNDKIFKGEILLFFVNKSNSVGGMETLCPPAELNSGMFELLILKKVSPKTLFQLFAS
IKKGTHLSSPHVIHARTNKVTINSEADLNVSYDGVYGGKAPYTLEVIPEALEVFADTNRISSRLRG

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=243, Percent_Identity=30.8641975308642, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 33271; Mature: 33271

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKAMIIYNPAAGKNKFRKLLPDAEKILTEADFEVTLVPSTPAPKSTTFIAKQAAEAGF
CCCCEEEEECCCCCHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCC
DVVIAAGGDGTVNEVVNGLMQVDTPPKLGVLPVGTTNDYARALNFAKNPLEALRIIAKQE
EEEEEECCCCCHHHHHHHHEECCCCCCCCEEECCCCHHHHHHHHHCCCHHHHHHHHHCCC
TIRVDIGKANETEFFINNAAGGKITEITYAVKESMKSKWGRLAYLFSGLTVLPKLSPVYV
EEEEEECCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEE
EIAYNDKIFKGEILLFFVNKSNSVGGMETLCPPAELNSGMFELLILKKVSPKTLFQLFAS
EEEECCEEEECEEEEEEEECCCCCCCHHHCCCHHHHCCCCEEEEEEECCCHHHHHHHHHH
IKKGTHLSSPHVIHARTNKVTINSEADLNVSYDGVYGGKAPYTLEVIPEALEVFADTNRI
HHCCCCCCCCEEEEEECCEEEECCCCCCEEEECCEECCCCCEEEEHHHHHHHHHHCHHHH
SSRLRG
HHHHCC
>Mature Secondary Structure
MQKKAMIIYNPAAGKNKFRKLLPDAEKILTEADFEVTLVPSTPAPKSTTFIAKQAAEAGF
CCCCEEEEECCCCCHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCC
DVVIAAGGDGTVNEVVNGLMQVDTPPKLGVLPVGTTNDYARALNFAKNPLEALRIIAKQE
EEEEEECCCCCHHHHHHHHEECCCCCCCCEEECCCCHHHHHHHHHCCCHHHHHHHHHCCC
TIRVDIGKANETEFFINNAAGGKITEITYAVKESMKSKWGRLAYLFSGLTVLPKLSPVYV
EEEEEECCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEE
EIAYNDKIFKGEILLFFVNKSNSVGGMETLCPPAELNSGMFELLILKKVSPKTLFQLFAS
EEEECCEEEECEEEEEEEECCCCCCCHHHCCCHHHHCCCCEEEEEEECCCHHHHHHHHHH
IKKGTHLSSPHVIHARTNKVTINSEADLNVSYDGVYGGKAPYTLEVIPEALEVFADTNRI
HHCCCCCCCCEEEEEECCEEEECCCCCCEEEECCEECCCCCEEEEHHHHHHHHHHCHHHH
SSRLRG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]