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The map label for this gene is tagO [H]

Identifier: 226225067

GI number: 226225067

Start: 2570104

End: 2571156

Strand: Reverse

Name: tagO [H]

Synonym: Lm4b_02488

Alternate gene names: 226225067

Gene position: 2571156-2570104 (Counterclockwise)

Preceding gene: 226225068

Following gene: 226225063

Centisome position: 88.27

GC content: 35.23

Gene sequence:

>1053_bases
TTGCTAATATGGAGTATATTGATTAGCTTTGCAGTTGGTATCATTATGGTGCCGATAATTAGGAAGTTTGCTTTCCGAAT
TAATGCGGTTGATACACCGCGTGAAAGACACAAACATACAAAAACTACAGCCACTTTAGGTGGATTAGCTATTTTTATAA
GTTTTTCGGTAGGGATGATCTTAGCCCCAATAGATAAATCAGGTTTCTTGCCAATTTATTTCGGAGCATTAATTATTATT
GCGACAGGCTTTATAGATGATATATTTGATTTATCACCAAAATGGAAAATGTTAGGACAGATTTTAGCTGCTCTTTGCGT
AACTGTCTGGGGAGACATTACTATTAACTTTATTAACGTCCCATTCTACGGACAAGTAGATTTTGGGTATTTTGCGATTC
CATTATCGATTATTTGGATTGTTGCAATTGTGAATGCGTTAAACTTAATTGATGGATTAGATGGTCTCGCTGGTGGTATT
TCTATTATTGCTTTAATGACCATTGCAGGTATGGCTTTGCTTTTGAAAGATGTTTTTGTTGCGCCGGTTGCGTTAATTCT
TGTAGCAGCAGTTGCGGCTTTCTTGATTTATAATTTCCCGCCTGCTAGTATTTTTATGGGAGACACTGGAGCGTTATTCC
TTGGTTATATGATTGCTGTTTTATCACTTATGGGTTTTAAAAACGTTACTTTCATCTCATTGTTAGTACCACTTATTATT
TTAGGTGTACCTTTGTCAGATACTTTTTTTGCTATTGTAAGAAGACTAAAAGAGCGGATACCGATATCTTCAGCGGATAG
ATCTCATATTCACCATAGGCTGATGGCGCTAGGATTTACAGAGAAACAGACGGTGTTACTAATCTACTGTATGGCATTAC
TATTCTCTATGACGGGCTTCGTATTTTCGTTTTCCACTACTTGGGGAGCAATGTTATTACTGTTATTATTAATTTTTAGT
ATTGAGATTATTGTTGAATTTATTGGGTTAATTGGTGAGGATTACCGACCAATCTTAAATTTACTTCAAAAAATACATCG
AAAACGAAAATAA

Upstream 100 bases:

>100_bases
GGTCAAGGGGTTGGAGTTGCTTTAGATTTAGATGTGCTACAAAAATATACTAAATCAACAGAAAGAATTTTGCTAAATAA
AGGATGGAGCTGAAAAAATA

Downstream 100 bases:

>100_bases
AAAGACCAACAACTATTTATTAGTTGTTGGTCTTTTTTTAATTAGAAGTGCTAGTTGTGTTAGAACTTTCGTCTGAATAC
GGTGCTGCACCAGGGAATGG

Product: TagO teichoic acid linkage unit synthesis protein

Products: UMP; Undecaprenyl N-Acetyl-Alpha-D-Glucosaminyl Pyrophosphate. [C]

Alternate protein names: UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase; Undecaprenyl-Phosphate GlcNAc-1-phosphate transferase [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MLIWSILISFAVGIIMVPIIRKFAFRINAVDTPRERHKHTKTTATLGGLAIFISFSVGMILAPIDKSGFLPIYFGALIII
ATGFIDDIFDLSPKWKMLGQILAALCVTVWGDITINFINVPFYGQVDFGYFAIPLSIIWIVAIVNALNLIDGLDGLAGGI
SIIALMTIAGMALLLKDVFVAPVALILVAAVAAFLIYNFPPASIFMGDTGALFLGYMIAVLSLMGFKNVTFISLLVPLII
LGVPLSDTFFAIVRRLKERIPISSADRSHIHHRLMALGFTEKQTVLLIYCMALLFSMTGFVFSFSTTWGAMLLLLLLIFS
IEIIVEFIGLIGEDYRPILNLLQKIHRKRK

Sequences:

>Translated_350_residues
MLIWSILISFAVGIIMVPIIRKFAFRINAVDTPRERHKHTKTTATLGGLAIFISFSVGMILAPIDKSGFLPIYFGALIII
ATGFIDDIFDLSPKWKMLGQILAALCVTVWGDITINFINVPFYGQVDFGYFAIPLSIIWIVAIVNALNLIDGLDGLAGGI
SIIALMTIAGMALLLKDVFVAPVALILVAAVAAFLIYNFPPASIFMGDTGALFLGYMIAVLSLMGFKNVTFISLLVPLII
LGVPLSDTFFAIVRRLKERIPISSADRSHIHHRLMALGFTEKQTVLLIYCMALLFSMTGFVFSFSTTWGAMLLLLLLIFS
IEIIVEFIGLIGEDYRPILNLLQKIHRKRK
>Mature_350_residues
MLIWSILISFAVGIIMVPIIRKFAFRINAVDTPRERHKHTKTTATLGGLAIFISFSVGMILAPIDKSGFLPIYFGALIII
ATGFIDDIFDLSPKWKMLGQILAALCVTVWGDITINFINVPFYGQVDFGYFAIPLSIIWIVAIVNALNLIDGLDGLAGGI
SIIALMTIAGMALLLKDVFVAPVALILVAAVAAFLIYNFPPASIFMGDTGALFLGYMIAVLSLMGFKNVTFISLLVPLII
LGVPLSDTFFAIVRRLKERIPISSADRSHIHHRLMALGFTEKQTVLLIYCMALLFSMTGFVFSFSTTWGAMLLLLLLIFS
IEIIVEFIGLIGEDYRPILNLLQKIHRKRK

Specific function: Catalyzes the formation of undecaprenyl-PP-N- acetylglucosamine. Involved in the synthesis of anionic cell-wall polymers as it mediates the initiation of the linkage unit formation that appears to be common to the two types of teichoic acids attached to t

COG id: COG0472

COG function: function code M; UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790218, Length=280, Percent_Identity=29.6428571428571, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1786275, Length=327, Percent_Identity=24.7706422018349, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000715
- InterPro:   IPR018480 [H]

Pfam domain/function: PF00953 Glycos_transf_4 [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 38575; Mature: 38575

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS01348 MRAY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIWSILISFAVGIIMVPIIRKFAFRINAVDTPRERHKHTKTTATLGGLAIFISFSVGMI
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAPIDKSGFLPIYFGALIIIATGFIDDIFDLSPKWKMLGQILAALCVTVWGDITINFINV
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEC
PFYGQVDFGYFAIPLSIIWIVAIVNALNLIDGLDGLAGGISIIALMTIAGMALLLKDVFV
CEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APVALILVAAVAAFLIYNFPPASIFMGDTGALFLGYMIAVLSLMGFKNVTFISLLVPLII
HHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LGVPLSDTFFAIVRRLKERIPISSADRSHIHHRLMALGFTEKQTVLLIYCMALLFSMTGF
HCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VFSFSTTWGAMLLLLLLIFSIEIIVEFIGLIGEDYRPILNLLQKIHRKRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLIWSILISFAVGIIMVPIIRKFAFRINAVDTPRERHKHTKTTATLGGLAIFISFSVGMI
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAPIDKSGFLPIYFGALIIIATGFIDDIFDLSPKWKMLGQILAALCVTVWGDITINFINV
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEC
PFYGQVDFGYFAIPLSIIWIVAIVNALNLIDGLDGLAGGISIIALMTIAGMALLLKDVFV
CEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APVALILVAAVAAFLIYNFPPASIFMGDTGALFLGYMIAVLSLMGFKNVTFISLLVPLII
HHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LGVPLSDTFFAIVRRLKERIPISSADRSHIHHRLMALGFTEKQTVLLIYCMALLFSMTGF
HCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VFSFSTTWGAMLLLLLLIFSIEIIVEFIGLIGEDYRPILNLLQKIHRKRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-Acetyl-D-Glucosamine; Undecaprenyl Phosphate [C]

Specific reaction: UDP-N-Acetyl-D-Glucosamine + Undecaprenyl Phosphate = UMP + Undecaprenyl N-Acetyl-Alpha-D-Glucosaminyl Pyrophosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12101296; 9384377 [H]