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The map label for this gene is comFA

Identifier: 226225061

GI number: 226225061

Start: 2564192

End: 2565511

Strand: Reverse

Name: comFA

Synonym: Lm4b_02482

Alternate gene names: 226225061

Gene position: 2565511-2564192 (Counterclockwise)

Preceding gene: 226225062

Following gene: 226225060

Centisome position: 88.08

GC content: 39.24

Gene sequence:

>1320_bases
ATGGACATCTTCCCGGGAAGGCTTTATCAAGAACGTGAAATAGAGAGTAAAGAGGGCTTAAGAGAAGTAGTAGCAATAGA
ATCAAACAAATGCTTTCGATGTGGAAATACGGATGTGCAGTTATTTGGCAAAATGTCCTGTGCATTATGTAAGAGAGAGG
ATTGTATTTATTGCAGAAATTGCATTGTGATGGGACGAGTAAATAGTTGCCAAAAATTATACTATCAACATGCTAGGTTA
TTACTAGAGACAAAGGAAGTATTATTGAGGTGGGAAGGAGCTTTGTCAGCGGGACAGCGAAAAGCATCCAACGCGATAGT
CGTTAATTTAAAAGCAAAAACGGATATGTTGCTATGGGCAGTTGCCGGATCTGGAAAAACGGAGATGATGTTTGAAGGAA
TGGACTGGGCTTTGCGACAAGGCTTTAGAATTTGTGTTGCTTCTCCGAGAGTGGATGTTTGTTTAGAGCTTCTTCCACGT
TTAAAAGAAGCTTTCCCTAGCATAGATATTGTTTGTCTATATGGAGACTCAAAAGATAACTACCAAGGAGAGCAGTTCGT
GATAGCGACGACCCATCAGCTTATTCGCTTTTATGAAGCGTTTCAAGTTATTTTTATTGATGAAGTAGATGCATTTCCTT
ATGCGAAAGATCCTTTTTTAGAATATGCCGTTATGAAAGCCAGAACAAAAGAAGGTTCAACAATAATAATCACAGCAACC
CCAGAGAAAAAGTGGCAAGAAGAGTGTGTAAGAGGAAAACGTAATTTTGTGAAAATTCCCGGACGATATCACCGAAAGAA
ACTGCCTGTGCCGAGAACGTGCTGGATTGGCCCGTGGAAAAAGAATCTAGCTAAAGGGAAAATAACTCCTAAGCTTATCC
AGTGGATGAATGATGTGAAGAAGAAAGATCAGCCGGCTTTAATTTTCTTTCCAGAAATTGATGCTATGAATAAATTTGCA
AATGCACTTGAAAATAAAGGCTATGCCAGCCCAGTGACAGTTCATTCAGCCGATGAATTGCGTAAAGAGAAAGTGGAATG
GCTACGAAAAGGGAAAATCAAGCTGTTATTAACAACTACAATTTTAGAGAGAGGTGTTACTTTTACAGATGTGCAAGTAG
CGGTGTTTGGAAGTGAAGCTCGTATTTTTACGGAAGCTGCTTTAGTTCAAATTTCAGGAAGGGCAGGTAGGAAATTATCT
CATCCGACTGGAGACGTTTGCTTTTTTCATTACGGTAAAACAATAGAAATGAAGCAAGCTATCTCCCACATTCAAAAAAT
GAATCAACAAGGAGTGATAGAAGGGATGCTAGATGACTAA

Upstream 100 bases:

>100_bases
AGAAATACTTGTTTTGTTTATAAGTATGCGGTCGAAAACAAGGATTTCGTTTCTGCAATAAAAAAAGAAAAAGTGTATAA
CTAAGGGGAGGTGAATACAG

Downstream 100 bases:

>100_bases
TTGCTTACTTTGTTTTCAACCAGTTAGGCAAAGCGCCAGTTGGGAAATAAGTTGGTTATTTGAACCTTTGCCAATTTGTT
GTGATGCATGCTTAGCGGGA

Product: late competence protein comFA

Products: NA

Alternate protein names: ATP-dependent helicase ComFA [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MDIFPGRLYQEREIESKEGLREVVAIESNKCFRCGNTDVQLFGKMSCALCKREDCIYCRNCIVMGRVNSCQKLYYQHARL
LLETKEVLLRWEGALSAGQRKASNAIVVNLKAKTDMLLWAVAGSGKTEMMFEGMDWALRQGFRICVASPRVDVCLELLPR
LKEAFPSIDIVCLYGDSKDNYQGEQFVIATTHQLIRFYEAFQVIFIDEVDAFPYAKDPFLEYAVMKARTKEGSTIIITAT
PEKKWQEECVRGKRNFVKIPGRYHRKKLPVPRTCWIGPWKKNLAKGKITPKLIQWMNDVKKKDQPALIFFPEIDAMNKFA
NALENKGYASPVTVHSADELRKEKVEWLRKGKIKLLLTTTILERGVTFTDVQVAVFGSEARIFTEAALVQISGRAGRKLS
HPTGDVCFFHYGKTIEMKQAISHIQKMNQQGVIEGMLDD

Sequences:

>Translated_439_residues
MDIFPGRLYQEREIESKEGLREVVAIESNKCFRCGNTDVQLFGKMSCALCKREDCIYCRNCIVMGRVNSCQKLYYQHARL
LLETKEVLLRWEGALSAGQRKASNAIVVNLKAKTDMLLWAVAGSGKTEMMFEGMDWALRQGFRICVASPRVDVCLELLPR
LKEAFPSIDIVCLYGDSKDNYQGEQFVIATTHQLIRFYEAFQVIFIDEVDAFPYAKDPFLEYAVMKARTKEGSTIIITAT
PEKKWQEECVRGKRNFVKIPGRYHRKKLPVPRTCWIGPWKKNLAKGKITPKLIQWMNDVKKKDQPALIFFPEIDAMNKFA
NALENKGYASPVTVHSADELRKEKVEWLRKGKIKLLLTTTILERGVTFTDVQVAVFGSEARIFTEAALVQISGRAGRKLS
HPTGDVCFFHYGKTIEMKQAISHIQKMNQQGVIEGMLDD
>Mature_439_residues
MDIFPGRLYQEREIESKEGLREVVAIESNKCFRCGNTDVQLFGKMSCALCKREDCIYCRNCIVMGRVNSCQKLYYQHARL
LLETKEVLLRWEGALSAGQRKASNAIVVNLKAKTDMLLWAVAGSGKTEMMFEGMDWALRQGFRICVASPRVDVCLELLPR
LKEAFPSIDIVCLYGDSKDNYQGEQFVIATTHQLIRFYEAFQVIFIDEVDAFPYAKDPFLEYAVMKARTKEGSTIIITAT
PEKKWQEECVRGKRNFVKIPGRYHRKKLPVPRTCWIGPWKKNLAKGKITPKLIQWMNDVKKKDQPALIFFPEIDAMNKFA
NALENKGYASPVTVHSADELRKEKVEWLRKGKIKLLLTTTILERGVTFTDVQVAVFGSEARIFTEAALVQISGRAGRKLS
HPTGDVCFFHYGKTIEMKQAISHIQKMNQQGVIEGMLDD

Specific function: Involved in transformation (competence for DNA uptake). Required for DNA uptake but not for binding. May provide the driving force for transport of DNA through an aqueous channel [H]

COG id: COG4098

COG function: function code L; Superfamily II DNA/RNA helicase required for DNA uptake (late competence protein)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935 [H]

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50118; Mature: 50118

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIFPGRLYQEREIESKEGLREVVAIESNKCFRCGNTDVQLFGKMSCALCKREDCIYCRN
CCCCCCCCCHHHCCCHHHHHHHHEEECCCCCEECCCCCCEEHHHHHHHHHCCCCCCEECC
CIVMGRVNSCQKLYYQHARLLLETKEVLLRWEGALSAGQRKASNAIVVNLKAKTDMLLWA
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECCCCEEEEE
VAGSGKTEMMFEGMDWALRQGFRICVASPRVDVCLELLPRLKEAFPSIDIVCLYGDSKDN
EECCCCCEEEECCCCHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
YQGEQFVIATTHQLIRFYEAFQVIFIDEVDAFPYAKDPFLEYAVMKARTKEGSTIIITAT
CCCCEEEEEHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECC
PEKKWQEECVRGKRNFVKIPGRYHRKKLPVPRTCWIGPWKKNLAKGKITPKLIQWMNDVK
CCHHHHHHHHCCCCCEEECCCHHHHCCCCCCCEEECCCHHHHHCCCCCCHHHHHHHHHHH
KKDQPALIFFPEIDAMNKFANALENKGYASPVTVHSADELRKEKVEWLRKGKIKLLLTTT
CCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCCEEEEEEEH
ILERGVTFTDVQVAVFGSEARIFTEAALVQISGRAGRKLSHPTGDVCFFHYGKTIEMKQA
HHHCCCEEEEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCEEEEECCCEEEHHHH
ISHIQKMNQQGVIEGMLDD
HHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MDIFPGRLYQEREIESKEGLREVVAIESNKCFRCGNTDVQLFGKMSCALCKREDCIYCRN
CCCCCCCCCHHHCCCHHHHHHHHEEECCCCCEECCCCCCEEHHHHHHHHHCCCCCCEECC
CIVMGRVNSCQKLYYQHARLLLETKEVLLRWEGALSAGQRKASNAIVVNLKAKTDMLLWA
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECCCCEEEEE
VAGSGKTEMMFEGMDWALRQGFRICVASPRVDVCLELLPRLKEAFPSIDIVCLYGDSKDN
EECCCCCEEEECCCCHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
YQGEQFVIATTHQLIRFYEAFQVIFIDEVDAFPYAKDPFLEYAVMKARTKEGSTIIITAT
CCCCEEEEEHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECC
PEKKWQEECVRGKRNFVKIPGRYHRKKLPVPRTCWIGPWKKNLAKGKITPKLIQWMNDVK
CCHHHHHHHHCCCCCEEECCCHHHHCCCCCCCEEECCCHHHHHCCCCCCHHHHHHHHHHH
KKDQPALIFFPEIDAMNKFANALENKGYASPVTVHSADELRKEKVEWLRKGKIKLLLTTT
CCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCCEEEEEEEH
ILERGVTFTDVQVAVFGSEARIFTEAALVQISGRAGRKLSHPTGDVCFFHYGKTIEMKQA
HHHCCCEEEEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCEEEEECCCEEEHHHH
ISHIQKMNQQGVIEGMLDD
HHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412657; 9384377 [H]