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The map label for this gene is spl

Identifier: 226225053

GI number: 226225053

Start: 2554638

End: 2555843

Strand: Reverse

Name: spl

Synonym: Lm4b_02474

Alternate gene names: 226225053

Gene position: 2555843-2554638 (Counterclockwise)

Preceding gene: 226225054

Following gene: 226225052

Centisome position: 87.75

GC content: 37.23

Gene sequence:

>1206_bases
TTGAAAAAGAATACGTTTATTGCGATTTCACTCGCAGCAGTTATCAGTTTGACGCCAGCTTTTACGACTAATGTTTTCGC
GGACGTGAATACAGACATTCAAAACCAAGATAAAAAAATTAACGACATCAAGTCTAAAAAAACAGATTTACAATCAGATC
TTTCTGGTTTAGTAGCAGACCTTGAGAAAGCTCAAGAAAAAGCTAAATCTCTACAAGGAGAATTTGATAAAACAGGCAAA
GAACTAAAAAAACTTAATGAAGATATTAAAAGTATCAATGAACGTATTAAAGAACGCGAAACTGTTTTAAAAGAACGTGC
TCGTGCGATGCAAAAAACTTCTAATTCTAATGCTTATCTTGAAGTTATCTTAGATGCAGAAAATCTTTCTGACTTAGTTG
GTCGTGTTTCTGCAGTTAACCAATTAGTTGATTCTGATAAATCTATTTTAGAAGATCAACAAAATGATGAAAAAGCTTTA
AAAACAAAACAAACTGCTGTTAAGAAAAAACAAGAAGATCAAGCAACAGCAATTCATGAATATGAAGCACAACAAAATAA
AATCGAAGCACAAAAAGCTGAAAAAGAAGCAATCGTTGCTCAACTAGCTTCTGATCAAGCAAGTGCTGAAAATGCAAAAG
CTGGTCTTGTAAGTGAACGTGATAAAGCAGCTAAAGAAGCTACAGCACGTGCGACAGCTTTACGTGAAGCAACATCTTCA
AACGTTGGACAAGAATCAAGTAGTTCAGCTACTTCTACATCAAGTGGAAAATCTAATACAACGAAAAATGTTGCATCAAA
TGATAACAATTCAGCACCAAGCGCAGCAACACCATCTAGTGGCGGATACTCAGCAATGATTGCTGCAGCTAACGCACAAC
TTGGAAAACCTTATAGCCTTGGAGCAACTGGACCAAGCGCATTTGACTGTTCAGGATTTACTTCTTATGCTTTCCGTGCA
GCTGGTGTTTCTCTTCCAAGAACATCTGGTGGACAATATGCAGCAGCATCTAAAATCAGCGCTAGCCAAGCAAAACCAGG
TGACTTAGTATTCTTTAACTATGGTAGCGGAATTGCTCACGTTGGAATTTATGTTGGTGGCGGTCAAATGATTAACGCTC
AAAACAATGGCGTTAAATATGACAACATCACTAGCGGTTACTGGGCTAAATATCTTGTAGGATATGGCCGCGTAGCTAAC
TTCTAA

Upstream 100 bases:

>100_bases
TATCCGCAGATTCTTGAAAGTCTAACTCAGAAAATTACTTAAATCGAAATAAAAATAATTTAAAAGAAAAAGCTAGGGAG
CTTTTTAGGAGGTAAACTTT

Downstream 100 bases:

>100_bases
TTATTATTGAAAAGTTAAAAAGCACCTTTGTCGTTTTAAACAAAGGTGCTTTTTTATATGGTATGGTAAAGGGAATTATG
AAGTTCCTAGTCAAATAGAG

Product: peptidoglycan lytic protein P45

Products: NA

Alternate protein names: Invasion-associated protein [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKKNTFIAISLAAVISLTPAFTTNVFADVNTDIQNQDKKINDIKSKKTDLQSDLSGLVADLEKAQEKAKSLQGEFDKTGK
ELKKLNEDIKSINERIKERETVLKERARAMQKTSNSNAYLEVILDAENLSDLVGRVSAVNQLVDSDKSILEDQQNDEKAL
KTKQTAVKKKQEDQATAIHEYEAQQNKIEAQKAEKEAIVAQLASDQASAENAKAGLVSERDKAAKEATARATALREATSS
NVGQESSSSATSTSSGKSNTTKNVASNDNNSAPSAATPSSGGYSAMIAAANAQLGKPYSLGATGPSAFDCSGFTSYAFRA
AGVSLPRTSGGQYAAASKISASQAKPGDLVFFNYGSGIAHVGIYVGGGQMINAQNNGVKYDNITSGYWAKYLVGYGRVAN
F

Sequences:

>Translated_401_residues
MKKNTFIAISLAAVISLTPAFTTNVFADVNTDIQNQDKKINDIKSKKTDLQSDLSGLVADLEKAQEKAKSLQGEFDKTGK
ELKKLNEDIKSINERIKERETVLKERARAMQKTSNSNAYLEVILDAENLSDLVGRVSAVNQLVDSDKSILEDQQNDEKAL
KTKQTAVKKKQEDQATAIHEYEAQQNKIEAQKAEKEAIVAQLASDQASAENAKAGLVSERDKAAKEATARATALREATSS
NVGQESSSSATSTSSGKSNTTKNVASNDNNSAPSAATPSSGGYSAMIAAANAQLGKPYSLGATGPSAFDCSGFTSYAFRA
AGVSLPRTSGGQYAAASKISASQAKPGDLVFFNYGSGIAHVGIYVGGGQMINAQNNGVKYDNITSGYWAKYLVGYGRVAN
F
>Mature_401_residues
MKKNTFIAISLAAVISLTPAFTTNVFADVNTDIQNQDKKINDIKSKKTDLQSDLSGLVADLEKAQEKAKSLQGEFDKTGK
ELKKLNEDIKSINERIKERETVLKERARAMQKTSNSNAYLEVILDAENLSDLVGRVSAVNQLVDSDKSILEDQQNDEKAL
KTKQTAVKKKQEDQATAIHEYEAQQNKIEAQKAEKEAIVAQLASDQASAENAKAGLVSERDKAAKEATARATALREATSS
NVGQESSSSATSTSSGKSNTTKNVASNDNNSAPSAATPSSGGYSAMIAAANAQLGKPYSLGATGPSAFDCSGFTSYAFRA
AGVSLPRTSGGQYAAASKISASQAKPGDLVFFNYGSGIAHVGIYVGGGQMINAQNNGVKYDNITSGYWAKYLVGYGRVAN
F

Specific function: This major extracellular protein may be involved in the invasion of non-professional phagocytic cells by Listeria [H]

COG id: COG3883

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=114, Percent_Identity=40.3508771929825, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786421, Length=120, Percent_Identity=35, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788501, Length=98, Percent_Identity=38.7755102040816, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788001, Length=113, Percent_Identity=38.0530973451327, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR003646
- InterPro:   IPR013247 [H]

Pfam domain/function: PF01476 LysM; PF00877 NLPC_P60; PF08239 SH3_3 [H]

EC number: NA

Molecular weight: Translated: 42570; Mature: 42570

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNTFIAISLAAVISLTPAFTTNVFADVNTDIQNQDKKINDIKSKKTDLQSDLSGLVAD
CCCCEEEEEEHHHHHHHCCHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LEKAQEKAKSLQGEFDKTGKELKKLNEDIKSINERIKERETVLKERARAMQKTSNSNAYL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
EVILDAENLSDLVGRVSAVNQLVDSDKSILEDQQNDEKALKTKQTAVKKKQEDQATAIHE
EEEECHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YEAQQNKIEAQKAEKEAIVAQLASDQASAENAKAGLVSERDKAAKEATARATALREATSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
NVGQESSSSATSTSSGKSNTTKNVASNDNNSAPSAATPSSGGYSAMIAAANAQLGKPYSL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEC
GATGPSAFDCSGFTSYAFRAAGVSLPRTSGGQYAAASKISASQAKPGDLVFFNYGSGIAH
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCCEEE
VGIYVGGGQMINAQNNGVKYDNITSGYWAKYLVGYGRVANF
EEEEECCCEEEECCCCCEECCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKNTFIAISLAAVISLTPAFTTNVFADVNTDIQNQDKKINDIKSKKTDLQSDLSGLVAD
CCCCEEEEEEHHHHHHHCCHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LEKAQEKAKSLQGEFDKTGKELKKLNEDIKSINERIKERETVLKERARAMQKTSNSNAYL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
EVILDAENLSDLVGRVSAVNQLVDSDKSILEDQQNDEKALKTKQTAVKKKQEDQATAIHE
EEEECHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YEAQQNKIEAQKAEKEAIVAQLASDQASAENAKAGLVSERDKAAKEATARATALREATSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
NVGQESSSSATSTSSGKSNTTKNVASNDNNSAPSAATPSSGGYSAMIAAANAQLGKPYSL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEC
GATGPSAFDCSGFTSYAFRAAGVSLPRTSGGQYAAASKISASQAKPGDLVFFNYGSGIAH
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCCEEE
VGIYVGGGQMINAQNNGVKYDNITSGYWAKYLVGYGRVANF
EEEEECCCEEEECCCCCEECCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1514809; 11679669; 1459966 [H]