Click here to switch to the map view.

The map label for this gene is cwlO [H]

Identifier: 226225052

GI number: 226225052

Start: 2553208

End: 2554518

Strand: Reverse

Name: cwlO [H]

Synonym: Lm4b_02473

Alternate gene names: 226225052

Gene position: 2554518-2553208 (Counterclockwise)

Preceding gene: 226225053

Following gene: 226225051

Centisome position: 87.7

GC content: 38.22

Gene sequence:

>1311_bases
ATGTTAAAAAAATATGGGGTAGTAACGATTCTTAGCCTATTGGTTATTTCCGCGCCACTAACTGGTGCGCACGCGGATAC
TATCAATGATATGCAAAAGCGCCAAAATGAAATTGAACAAAAAAAATCAGAACTAGATAAAAATATAGACTCGAAAAACT
CGGAATTAAATCATTTAGAAAGTGCTGAAAAAGACGCTGCAAAAGAATTAGAATCACTTATGAAAAGTCTTGATGCCACT
AACAAGAAATTAAAAGAGCAGGAAGATAAAGTGAGCTCTGAAAACGAGAAACTAAAGAAATTGCAAAAAGAAATGGAAAA
ACTGCGCAATGATATTCGCGATCGTCAAAAAGTACTTGATAATCGAGCGCGTGCTATCCAAACAACTGGAACAGCCACTT
CTTATTTAGATATGATTTTTGAAGCAGATGATTTTAAAGAACTTGTTGACCGCGTGACAGTTGTATCGGCAATTGTAAAA
GCGGACCAAAACATTATGCAAGACCAAAAAGATGATCAAGATAAACTAAAAGTAGCAGAATCTGCTTCTCAGAAAAAACT
AGAGAATCTAAAAGTACTTGCAGTGGAATTAGAAGTTTCGAAAAACAATATGGAAAGTCAAAAACAAGAAAAAAATGATT
TAGTTATGGCTCTAGCAAACAAAAAAGACTTAACTAAGAGCGAACAAACCCTTTTAGCTAGTGAACAAGGTGCGCTTACT
GATGAAGAAAAACGACTTGCTTCTAATATTGCAGGTGAAAAAGCAAAACAAGAGGCTGCAATTAAAGCCGCTGAAGAGAA
GCGAATGCAAGAAGCCGCAGCAGCAAGCGCTAAAAGTTCCTCTGTTGTTAAACAACAACCAAGTTCAGATTCAAGTGGAG
CTACAGAAACAGTAAGTTCCGGTGGAGGCCAATTTATTAAACCAGCTTCTGGTATATTAACTTCTGGATTTAGCGAACGA
ACAAATCCCGTGACTGGCAAATACGAATCCCACAAAGGTCAAGATATTGCAGGTGGCGGTACAGTTACTGTTTCAGCTGC
AGCATCAGGAACAGTTGTATTTTCAGGGTTTGGCGCATCAGGTAGTGGATTTGGTGGTTATGGTTATGTAGTAAAAATTG
ACCATGGCAATGGATTCCAGACACTATACGGACATATGCGTGCAGGTAGCTTGAAAGTAGTTACCGGCCAACAAGTTTCC
CAAGGCCAACCGATTGGTATTATGGGATCAACAGGTCAATCGACAGGACAACATCTACATTTTGAAATACATAAAAACGG
TATTCCAGTCGATCCAGCACCTTATATTTAA

Upstream 100 bases:

>100_bases
AAGCACCTTTGTCGTTTTAAACAAAGGTGCTTTTTTATATGGTATGGTAAAGGGAATTATGAAGTTCCTAGTCAAATAGA
GAATAGAAAAGGAGCTTAAA

Downstream 100 bases:

>100_bases
TTCAAAAACCATTGATGAAAAATCAATGGTTTTTTTCTTGTGTTGAAACGAACTGGAATAGGCTAATTGCAGATTTTATG
ATATGATACTACTATCAGTT

Product: cell wall binding protein

Products: NA

Alternate protein names: D-gamma-glutamyl-meso-diaminopimelyl DL-endopeptidase [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MLKKYGVVTILSLLVISAPLTGAHADTINDMQKRQNEIEQKKSELDKNIDSKNSELNHLESAEKDAAKELESLMKSLDAT
NKKLKEQEDKVSSENEKLKKLQKEMEKLRNDIRDRQKVLDNRARAIQTTGTATSYLDMIFEADDFKELVDRVTVVSAIVK
ADQNIMQDQKDDQDKLKVAESASQKKLENLKVLAVELEVSKNNMESQKQEKNDLVMALANKKDLTKSEQTLLASEQGALT
DEEKRLASNIAGEKAKQEAAIKAAEEKRMQEAAAASAKSSSVVKQQPSSDSSGATETVSSGGGQFIKPASGILTSGFSER
TNPVTGKYESHKGQDIAGGGTVTVSAAASGTVVFSGFGASGSGFGGYGYVVKIDHGNGFQTLYGHMRAGSLKVVTGQQVS
QGQPIGIMGSTGQSTGQHLHFEIHKNGIPVDPAPYI

Sequences:

>Translated_436_residues
MLKKYGVVTILSLLVISAPLTGAHADTINDMQKRQNEIEQKKSELDKNIDSKNSELNHLESAEKDAAKELESLMKSLDAT
NKKLKEQEDKVSSENEKLKKLQKEMEKLRNDIRDRQKVLDNRARAIQTTGTATSYLDMIFEADDFKELVDRVTVVSAIVK
ADQNIMQDQKDDQDKLKVAESASQKKLENLKVLAVELEVSKNNMESQKQEKNDLVMALANKKDLTKSEQTLLASEQGALT
DEEKRLASNIAGEKAKQEAAIKAAEEKRMQEAAAASAKSSSVVKQQPSSDSSGATETVSSGGGQFIKPASGILTSGFSER
TNPVTGKYESHKGQDIAGGGTVTVSAAASGTVVFSGFGASGSGFGGYGYVVKIDHGNGFQTLYGHMRAGSLKVVTGQQVS
QGQPIGIMGSTGQSTGQHLHFEIHKNGIPVDPAPYI
>Mature_436_residues
MLKKYGVVTILSLLVISAPLTGAHADTINDMQKRQNEIEQKKSELDKNIDSKNSELNHLESAEKDAAKELESLMKSLDAT
NKKLKEQEDKVSSENEKLKKLQKEMEKLRNDIRDRQKVLDNRARAIQTTGTATSYLDMIFEADDFKELVDRVTVVSAIVK
ADQNIMQDQKDDQDKLKVAESASQKKLENLKVLAVELEVSKNNMESQKQEKNDLVMALANKKDLTKSEQTLLASEQGALT
DEEKRLASNIAGEKAKQEAAIKAAEEKRMQEAAAASAKSSSVVKQQPSSDSSGATETVSSGGGQFIKPASGILTSGFSER
TNPVTGKYESHKGQDIAGGGTVTVSAAASGTVVFSGFGASGSGFGGYGYVVKIDHGNGFQTLYGHMRAGSLKVVTGQQVS
QGQPIGIMGSTGQSTGQHLHFEIHKNGIPVDPAPYI

Specific function: Shows a cell wall hydrolytic DL-endopeptidase activity [H]

COG id: COG3883

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C40 family [H]

Homologues:

Organism=Escherichia coli, GI87081989, Length=121, Percent_Identity=38.8429752066116, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082297, Length=134, Percent_Identity=32.8358208955224, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 47033; Mature: 47033

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKYGVVTILSLLVISAPLTGAHADTINDMQKRQNEIEQKKSELDKNIDSKNSELNHLE
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SAEKDAAKELESLMKSLDATNKKLKEQEDKVSSENEKLKKLQKEMEKLRNDIRDRQKVLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRARAIQTTGTATSYLDMIFEADDFKELVDRVTVVSAIVKADQNIMQDQKDDQDKLKVAE
HHHHEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
SASQKKLENLKVLAVELEVSKNNMESQKQEKNDLVMALANKKDLTKSEQTLLASEQGALT
HHHHHHHHCCEEEEEEEEECCCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHCCCCCCC
DEEKRLASNIAGEKAKQEAAIKAAEEKRMQEAAAASAKSSSVVKQQPSSDSSGATETVSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHC
GGGQFIKPASGILTSGFSERTNPVTGKYESHKGQDIAGGGTVTVSAAASGTVVFSGFGAS
CCCCEECCCHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEECCCCEEEEEECCCC
GSGFGGYGYVVKIDHGNGFQTLYGHMRAGSLKVVTGQQVSQGQPIGIMGSTGQSTGQHLH
CCCCCCEEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCCCCEEE
FEIHKNGIPVDPAPYI
EEEECCCCCCCCCCCC
>Mature Secondary Structure
MLKKYGVVTILSLLVISAPLTGAHADTINDMQKRQNEIEQKKSELDKNIDSKNSELNHLE
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SAEKDAAKELESLMKSLDATNKKLKEQEDKVSSENEKLKKLQKEMEKLRNDIRDRQKVLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRARAIQTTGTATSYLDMIFEADDFKELVDRVTVVSAIVKADQNIMQDQKDDQDKLKVAE
HHHHEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
SASQKKLENLKVLAVELEVSKNNMESQKQEKNDLVMALANKKDLTKSEQTLLASEQGALT
HHHHHHHHCCEEEEEEEEECCCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHCCCCCCC
DEEKRLASNIAGEKAKQEAAIKAAEEKRMQEAAAASAKSSSVVKQQPSSDSSGATETVSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHC
GGGQFIKPASGILTSGFSERTNPVTGKYESHKGQDIAGGGTVTVSAAASGTVVFSGFGAS
CCCCEECCCHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEECCCCEEEEEECCCC
GSGFGGYGYVVKIDHGNGFQTLYGHMRAGSLKVVTGQQVSQGQPIGIMGSTGQSTGQHLH
CCCCCCEEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCCCCEEE
FEIHKNGIPVDPAPYI
EEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA