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The map label for this gene is cls [H]

Identifier: 226225051

GI number: 226225051

Start: 2551636

End: 2553084

Strand: Reverse

Name: cls [H]

Synonym: Lm4b_02472

Alternate gene names: 226225051

Gene position: 2553084-2551636 (Counterclockwise)

Preceding gene: 226225052

Following gene: 226225050

Centisome position: 87.65

GC content: 35.82

Gene sequence:

>1449_bases
ATGGGACTGTTGGCTTATCTATTAGTGATTTTGCTAATTTTAAATGTATTCTTTGCGGCAGTGACGGTCTTCTTAGAAAG
ACGCGATACATCCGCCACTTGGGCTTGGTTGCTAGTCTTAACTTTTGTTCCGATATTTGGTTTCATTATCTATTTGATTT
TTGGGAGAAAATTATCAGGCAAAAAGATTTTCGATTGGAAGGGACAAGAAAAAATTGGGATACAGGAATCTACTGCCAAT
CAAATAGAAATGATACGGCAAAAAGAATTTCCATTTAGCGATCCGAATGTGAAAAAACATCGTGATTTAATCTATTTATT
ACTCGTAAACGATGGTGCTATTTTAACGCAAGACAATGAAGTGGAACTTTTTGTAGATGGACATGAGAAATTCGATGCGC
TAATTGCTGATATTGAAAAAGCGAAAGATCATATACATCTCATCTATTATATTTTTCATTCTGATGAACTAGGCAATCGC
TTAATGCGCGTGCTGGAAAGAAAAGCAGCAGAAGGTTTAAATGTGAAAATTATTTATGATGCAATGGGTTCAAGGACAAC
GAAAAAATCATTTTTCCGTACGTTTCAAAAAAATGGTGGTCTAGTTAGACCTTTTTTTCCGTCTAAATTACCATTGATTA
ACTTTCGTCTCAACTACCGAAATCACCGGAAATTGGCTATAATCGATGGAGATGTTGGTTATATTGGTGGTTTTAATATT
GGTGATGAATATCTTGGGGCATCGAAGAAATTTGGTTACTGGCGTGATACACATTTACGTGTACATGGAAAAGCTGTTTA
TGCGATGCAAACTCGCTTTATTATGGACTGGAATTCTGCATCATCCACACATAAAATTGATTACAAAGCGCGATACTTCC
CGACTTTTCATGGGAAAGGTCATACAAGTATGCAAATTGTTTCGAGTGGTCCTGATTCCGAATGGCAACAAATCAAAAAT
GGTTACATCAAAATGATCAATGCTGCTAAAAAAACAATTTATTTACAATCTCCTTATTTTATTCCAGATGCAAGTTTGCT
TGAAGCAATCAAAATCGCAGCACTTTCTGGGGTAGATGTCAGGGTTATGATTCCTAATAAGCCTGACCATGCTTTTGTTT
ACCGCGCAACTACTAACTACGCCGGTGAATTAATGGAAACGGGAGCGAAAATCTTTATTTATGATAATGGTTTTATCCAC
GCGAAAACACTCGTTGTAGATGGCGAAATTGCTTCAGTTGGAACCGCGAATATGGATTTCCGGAGTTTCCGACTTAATTT
TGAAGTAAATGCTTTTATTTATGAAAAACAAATGGTGCAAAAACTAGAAGATGCCTTTTTGGAAGATATTCTAAAATCGT
ATCAACTAACACCTGAATTATATGCAAAACGATCTTTATGGATTAAATTTAAAGAAGCGGTAAGCCGACTTTTATCGCCT
ATATTATAG

Upstream 100 bases:

>100_bases
CAATGGTTTTTTTCTTGTGTTGAAACGAACTGGAATAGGCTAATTGCAGATTTTATGATATGATACTACTATCAGTTTGT
GTAAGAAAAGGGGGAACACA

Downstream 100 bases:

>100_bases
AAGTATTAGGGAAAGCTGGTGTAGAAAATGGATGTTTTTGTTGAAATACTAAATGGAATAGGGAGACTACTTCTACAACC
AGCATTGTATGTAGGAATTC

Product: cardiolipin synthase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MGLLAYLLVILLILNVFFAAVTVFLERRDTSATWAWLLVLTFVPIFGFIIYLIFGRKLSGKKIFDWKGQEKIGIQESTAN
QIEMIRQKEFPFSDPNVKKHRDLIYLLLVNDGAILTQDNEVELFVDGHEKFDALIADIEKAKDHIHLIYYIFHSDELGNR
LMRVLERKAAEGLNVKIIYDAMGSRTTKKSFFRTFQKNGGLVRPFFPSKLPLINFRLNYRNHRKLAIIDGDVGYIGGFNI
GDEYLGASKKFGYWRDTHLRVHGKAVYAMQTRFIMDWNSASSTHKIDYKARYFPTFHGKGHTSMQIVSSGPDSEWQQIKN
GYIKMINAAKKTIYLQSPYFIPDASLLEAIKIAALSGVDVRVMIPNKPDHAFVYRATTNYAGELMETGAKIFIYDNGFIH
AKTLVVDGEIASVGTANMDFRSFRLNFEVNAFIYEKQMVQKLEDAFLEDILKSYQLTPELYAKRSLWIKFKEAVSRLLSP
IL

Sequences:

>Translated_482_residues
MGLLAYLLVILLILNVFFAAVTVFLERRDTSATWAWLLVLTFVPIFGFIIYLIFGRKLSGKKIFDWKGQEKIGIQESTAN
QIEMIRQKEFPFSDPNVKKHRDLIYLLLVNDGAILTQDNEVELFVDGHEKFDALIADIEKAKDHIHLIYYIFHSDELGNR
LMRVLERKAAEGLNVKIIYDAMGSRTTKKSFFRTFQKNGGLVRPFFPSKLPLINFRLNYRNHRKLAIIDGDVGYIGGFNI
GDEYLGASKKFGYWRDTHLRVHGKAVYAMQTRFIMDWNSASSTHKIDYKARYFPTFHGKGHTSMQIVSSGPDSEWQQIKN
GYIKMINAAKKTIYLQSPYFIPDASLLEAIKIAALSGVDVRVMIPNKPDHAFVYRATTNYAGELMETGAKIFIYDNGFIH
AKTLVVDGEIASVGTANMDFRSFRLNFEVNAFIYEKQMVQKLEDAFLEDILKSYQLTPELYAKRSLWIKFKEAVSRLLSP
IL
>Mature_481_residues
GLLAYLLVILLILNVFFAAVTVFLERRDTSATWAWLLVLTFVPIFGFIIYLIFGRKLSGKKIFDWKGQEKIGIQESTANQ
IEMIRQKEFPFSDPNVKKHRDLIYLLLVNDGAILTQDNEVELFVDGHEKFDALIADIEKAKDHIHLIYYIFHSDELGNRL
MRVLERKAAEGLNVKIIYDAMGSRTTKKSFFRTFQKNGGLVRPFFPSKLPLINFRLNYRNHRKLAIIDGDVGYIGGFNIG
DEYLGASKKFGYWRDTHLRVHGKAVYAMQTRFIMDWNSASSTHKIDYKARYFPTFHGKGHTSMQIVSSGPDSEWQQIKNG
YIKMINAAKKTIYLQSPYFIPDASLLEAIKIAALSGVDVRVMIPNKPDHAFVYRATTNYAGELMETGAKIFIYDNGFIHA
KTLVVDGEIASVGTANMDFRSFRLNFEVNAFIYEKQMVQKLEDAFLEDILKSYQLTPELYAKRSLWIKFKEAVSRLLSPI
L

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=485, Percent_Identity=27.4226804123711, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1787007, Length=357, Percent_Identity=24.9299719887955, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 55363; Mature: 55232

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLLAYLLVILLILNVFFAAVTVFLERRDTSATWAWLLVLTFVPIFGFIIYLIFGRKLSG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KKIFDWKGQEKIGIQESTANQIEMIRQKEFPFSDPNVKKHRDLIYLLLVNDGAILTQDNE
CEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCC
VELFVDGHEKFDALIADIEKAKDHIHLIYYIFHSDELGNRLMRVLERKAAEGLNVKIIYD
EEEEECCCHHHHHHHHHHHHCCCEEEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEE
AMGSRTTKKSFFRTFQKNGGLVRPFFPSKLPLINFRLNYRNHRKLAIIDGDVGYIGGFNI
CCCCCCHHHHHHHHHHHCCCEECCCCCCCCCEEEEEECCCCCCEEEEEECCCCEECCCCC
GDEYLGASKKFGYWRDTHLRVHGKAVYAMQTRFIMDWNSASSTHKIDYKARYFPTFHGKG
CHHHCCCCCCCCCEECCEEEEECEEEEEEEEEEEEEECCCCCCEEEEEEEEEEEEECCCC
HTSMQIVSSGPDSEWQQIKNGYIKMINAAKKTIYLQSPYFIPDASLLEAIKIAALSGVDV
CCEEEEEECCCCHHHHHHHCCHHEEEECCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEE
RVMIPNKPDHAFVYRATTNYAGELMETGAKIFIYDNGFIHAKTLVVDGEIASVGTANMDF
EEECCCCCCCEEEEEECCCCHHHHHHCCCEEEEEECCEEEEEEEEECCCEEECCCCCCCE
RSFRLNFEVNAFIYEKQMVQKLEDAFLEDILKSYQLTPELYAKRSLWIKFKEAVSRLLSP
EEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEHHHHHHHHHHC
IL
CC
>Mature Secondary Structure 
GLLAYLLVILLILNVFFAAVTVFLERRDTSATWAWLLVLTFVPIFGFIIYLIFGRKLSG
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KKIFDWKGQEKIGIQESTANQIEMIRQKEFPFSDPNVKKHRDLIYLLLVNDGAILTQDNE
CEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCC
VELFVDGHEKFDALIADIEKAKDHIHLIYYIFHSDELGNRLMRVLERKAAEGLNVKIIYD
EEEEECCCHHHHHHHHHHHHCCCEEEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEE
AMGSRTTKKSFFRTFQKNGGLVRPFFPSKLPLINFRLNYRNHRKLAIIDGDVGYIGGFNI
CCCCCCHHHHHHHHHHHCCCEECCCCCCCCCEEEEEECCCCCCEEEEEECCCCEECCCCC
GDEYLGASKKFGYWRDTHLRVHGKAVYAMQTRFIMDWNSASSTHKIDYKARYFPTFHGKG
CHHHCCCCCCCCCEECCEEEEECEEEEEEEEEEEEEECCCCCCEEEEEEEEEEEEECCCC
HTSMQIVSSGPDSEWQQIKNGYIKMINAAKKTIYLQSPYFIPDASLLEAIKIAALSGVDV
CCEEEEEECCCCHHHHHHHCCHHEEEECCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEE
RVMIPNKPDHAFVYRATTNYAGELMETGAKIFIYDNGFIHAKTLVVDGEIASVGTANMDF
EEECCCCCCCEEEEEECCCCHHHHHHCCCEEEEEECCEEEEEEEEECCCEEECCCCCCCE
RSFRLNFEVNAFIYEKQMVQKLEDAFLEDILKSYQLTPELYAKRSLWIKFKEAVSRLLSP
EEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEHHHHHHHHHHC
IL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]