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The map label for this gene is pit [H]

Identifier: 226224857

GI number: 226224857

Start: 2344364

End: 2345362

Strand: Direct

Name: pit [H]

Synonym: Lm4b_02276

Alternate gene names: 226224857

Gene position: 2344364-2345362 (Clockwise)

Preceding gene: 226224856

Following gene: 226224858

Centisome position: 80.49

GC content: 41.04

Gene sequence:

>999_bases
ATGGAAGGAATGTTTCTCATCACCCTCGTCATCGTACTTGCCGCGCTAGCATTTGACCTGATCAACGGGTTTCATGATAC
AGCTAACGCAATTGCGACTAGTGTCTCTACAAAAGCCTTAAAACCACGACATGCGATTATTCTTGCTGCCGTAATGAACT
TTGTGGGTGCTATTTCATTCACAGGGGTTGCTAAAACAATTACAAAAGACATTGTTAACCCATTCGACTTAGATCACGGC
GAACTTGTTATTTTAGCAGCATTACTTTCAGCTATTGCTTGGAACTTAATCACTTGGTATTTCGGAATTCCTAGTAGTTC
CTCCCATGCCTTGATTGGATCCATCGCCGGTGCAGCCATTGCATCAGCCGGATTTGCAGCAATTGAATACAGCGGATTTA
CTAAAATCATTGTTGGTTTATTAGTATCTCCTGTACTTGCTTTCGTAGTCGGTTACACGATATATTCACTCTTCAAGGTT
TTCCTGAAGAACTTAAACTTAGCCACGACCAATCGGCGCTTCCGGATGATTCAAGTCGGAACTGCTGCGCTACAATCTTA
CACACACGGAACTAATGATGCACAAAAATCAATGGGGATTATCACAATGGCATTAATTGCTAGTGGTTTCCAAACAACCG
ATGATGTGCAATTATGGGTTCAAGTATCCTGTGCGATTGCCATGGCGATTGGTACGAGTATTGGTGGTTGGAAAATCATC
AAAACTGTTGGCGGTAAAATCATGAAAATCAAACCAGTTAATGGTGTAGCGGCTGATTTAAGTTCCGTTATCATTATTTT
CGGTGCTACTTTCATTCATTTACCAGTTAGTACAACACACGTAATCAGCTCTTCTATCCTTGGTGTTGGAACAGCTCACC
GTGTCAAAGGTGTCAAATGGGATACTGCCCAACGTATGATTATTACTTGGGTTATCACACTTCCTATTTCAGCAACAATT
GCAGCACTTATCTTCTATGTACTAAGATTTATTCTTTAA

Upstream 100 bases:

>100_bases
GAAGTTTATGAAAAATTAGAAGACATTGCTGATAGTTGTCAAAGCGTTGCGAATACGCTTGAATCAATTGTCATGAAAAA
TGCGTAAGGGGCCTAGATAG

Downstream 100 bases:

>100_bases
TATTATTTAGAGCCTTGCTAACGCGAGGCTATTTTTATACATTTTTTTGAATAAAACTAATAAATATAATATAAAACGAG
ATTATTTTAAAAACATATTG

Product: low-affinity inorganic phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MEGMFLITLVIVLAALAFDLINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAISFTGVAKTITKDIVNPFDLDHG
ELVILAALLSAIAWNLITWYFGIPSSSSHALIGSIAGAAIASAGFAAIEYSGFTKIIVGLLVSPVLAFVVGYTIYSLFKV
FLKNLNLATTNRRFRMIQVGTAALQSYTHGTNDAQKSMGIITMALIASGFQTTDDVQLWVQVSCAIAMAIGTSIGGWKII
KTVGGKIMKIKPVNGVAADLSSVIIIFGATFIHLPVSTTHVISSSILGVGTAHRVKGVKWDTAQRMIITWVITLPISATI
AALIFYVLRFIL

Sequences:

>Translated_332_residues
MEGMFLITLVIVLAALAFDLINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAISFTGVAKTITKDIVNPFDLDHG
ELVILAALLSAIAWNLITWYFGIPSSSSHALIGSIAGAAIASAGFAAIEYSGFTKIIVGLLVSPVLAFVVGYTIYSLFKV
FLKNLNLATTNRRFRMIQVGTAALQSYTHGTNDAQKSMGIITMALIASGFQTTDDVQLWVQVSCAIAMAIGTSIGGWKII
KTVGGKIMKIKPVNGVAADLSSVIIIFGATFIHLPVSTTHVISSSILGVGTAHRVKGVKWDTAQRMIITWVITLPISATI
AALIFYVLRFIL
>Mature_332_residues
MEGMFLITLVIVLAALAFDLINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAISFTGVAKTITKDIVNPFDLDHG
ELVILAALLSAIAWNLITWYFGIPSSSSHALIGSIAGAAIASAGFAAIEYSGFTKIIVGLLVSPVLAFVVGYTIYSLFKV
FLKNLNLATTNRRFRMIQVGTAALQSYTHGTNDAQKSMGIITMALIASGFQTTDDVQLWVQVSCAIAMAIGTSIGGWKII
KTVGGKIMKIKPVNGVAADLSSVIIIFGATFIHLPVSTTHVISSSILGVGTAHRVKGVKWDTAQRMIITWVITLPISATI
AALIFYVLRFIL

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=162, Percent_Identity=28.3950617283951, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI5803173, Length=160, Percent_Identity=30, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789360, Length=241, Percent_Identity=30.7053941908714, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789907, Length=219, Percent_Identity=31.0502283105023, Blast_Score=107, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI25146401, Length=162, Percent_Identity=33.9506172839506, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71981576, Length=164, Percent_Identity=33.5365853658537, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17539280, Length=166, Percent_Identity=26.5060240963855, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17536725, Length=166, Percent_Identity=25.3012048192771, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI32566716, Length=166, Percent_Identity=28.9156626506024, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319773, Length=155, Percent_Identity=30.3225806451613, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35255; Mature: 35255

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGMFLITLVIVLAALAFDLINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAISF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TGVAKTITKDIVNPFDLDHGELVILAALLSAIAWNLITWYFGIPSSSSHALIGSIAGAAI
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
ASAGFAAIEYSGFTKIIVGLLVSPVLAFVVGYTIYSLFKVFLKNLNLATTNRRFRMIQVG
HHCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEEEHH
TAALQSYTHGTNDAQKSMGIITMALIASGFQTTDDVQLWVQVSCAIAMAIGTSIGGWKII
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
KTVGGKIMKIKPVNGVAADLSSVIIIFGATFIHLPVSTTHVISSSILGVGTAHRVKGVKW
HHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHCCCCHHHHCCCCC
DTAQRMIITWVITLPISATIAALIFYVLRFIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MEGMFLITLVIVLAALAFDLINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAISF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TGVAKTITKDIVNPFDLDHGELVILAALLSAIAWNLITWYFGIPSSSSHALIGSIAGAAI
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
ASAGFAAIEYSGFTKIIVGLLVSPVLAFVVGYTIYSLFKVFLKNLNLATTNRRFRMIQVG
HHCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEEEHH
TAALQSYTHGTNDAQKSMGIITMALIASGFQTTDDVQLWVQVSCAIAMAIGTSIGGWKII
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
KTVGGKIMKIKPVNGVAADLSSVIIIFGATFIHLPVSTTHVISSSILGVGTAHRVKGVKW
HHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHCCCCHHHHCCCCC
DTAQRMIITWVITLPISATIAALIFYVLRFIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]