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The map label for this gene is baiH [H]

Identifier: 226224843

GI number: 226224843

Start: 2331421

End: 2333346

Strand: Direct

Name: baiH [H]

Synonym: Lm4b_02262

Alternate gene names: 226224843

Gene position: 2331421-2333346 (Clockwise)

Preceding gene: 226224842

Following gene: 226224844

Centisome position: 80.04

GC content: 43.51

Gene sequence:

>1926_bases
TTGAAATTTAATTCGATGTTTTCACCTATTGATATCGGGCCGATGAAGGTGCCTAACAGATTTGTCGTATCGCCAATGTG
TAACAACTATGCAAATACAGATGGAACTTTATCAGATACTTCCCTTGCTTATTACAAAGAACGAGCACTTGGGGGTTTTG
GGTTTATTACTTTTGAAGCAACGGTTGTAGATGTACGCGCAAAAGGTGGCGCAAATAAAGCATGCCTATATAGTGATCAT
CAAATTGCTAGTTTTAAACGAGTGATTGATGTATGTCACGATGCTGGAGCAAAAATTTCTGTTCAGTTACAGCACGCTGG
ACCAGAAGGAAATTCCAAAGTATCTGGTTATCCTTTAAGAGCAGCCTCAGCAATCGCCTCTGCTGCAGGGCGTAATACTC
CGGAAGCCATTTCGCGTGAAGAACTTTATGAGTTGATTGAACTTTACGGAGAAGCTGCTTTACGAGCAAAAAAAGCGGGC
GCCGATGCGGTTGAAGTTCACTGCGCACATGGTTATCTAGTAAGTAGTTTCTTATCTGCACGGACGAACAAACGTGTGGA
CGAGTTTGGTGGCTGTTTTGAAAACCGAATGCGATTACCTCGGCTTATTATTGAAAGTATTCGTAAACGTGTCGGTCATT
CCATCGCAATTCTTTGCCGAATTAATAGCACGGATGGTGTGGAAGGCGGACTAAGTGTTCAAGATAGCGCAACAGTTGCA
GCTTATTTAGAAGATTGTGGTTTGGATGGTTTGCATGTTTCTCGTAGCGTCCATATTCGTGATGAATACATGTGGGCGCC
AACCGTATTGCATGCAGGATTTAGCTCTGATCTTGTAACACAAATCAAACGCGCTGTTTCTATTCCTGTAATTACTGTGG
GTCGTTTCACAGAGCCGCATTACGCGGAATTAATGGTTCGTGAAGGCCGCGCTGACTTGGTTGCTTTCGGGCGTCAGTCT
TTAGCAGATCCAGAAACACCAAACAAAGCTGCTGCTGGAAAACTAGACGAATTACTTCCTTGCATCGCTTGTCTTCAAGG
TTGCGTTGCTAATATGTATGCCGGAAAACCCATTACTTGTTTAGTAAATCCGTTACTTGGACGTGAATCCGAAGCTTATT
CACCTAAAACTCCAAGTAAAAAAGTAGTTGTCATTGGCGGTGGTGTCGGTGGGCTTTATGCTGGATGGATGGCTGGATCC
CGTGGTCATGATGTGACAGTTTATGAAGCATCAGATATGATTGGCGGTCAAATGCGCTTAGCTGCCTATCCTCCTGGTAA
AGGCGACTTAACAAACATGGTTCGAAGTTACATCAAAAAGTGTGAACAGTTCGATGTCGAAATTAAAACAAACACACCTG
TAACACCAGAACTTATTCAAGAAGTTGCCCCAGATGCCGTCATTATTGCAACTGGCGCCACTCCACTTGTTTTGCCGATA
CCAGGTATTGAGGATGCTGGACTTATCCACGCAGTTGACTTACTTGATGGGAAAGAGAAATGTGGTCAAAAAGTACTCGT
AGTTGGCGGAGGTATGGTTGGTAGTGAAACAGCTGCATTTTTAGGTGAAGCTGGTCATGACGTCACCGTTGTAGAACTTC
GCGATGAAGTCGGAGCAGACGTTATTTCTGAACATCGTAAATTCCTGATGCGTGATTTCGATGAATATAAAATCAAAAGT
GTTACGAATGCAAAAGTAACGAGTTTCTTTGAAGACGGGGTAACCTATTCCCTAGCGGATAACAAAGAACACCGAATCGA
CGGATTTGACTCTGTTGTTCTAGCAATGGGATCAAGAGCCTATAACCCACTGGAAGAAGCTATAAAAAAAATGGTACCCG
AAACCTATGTCATCGGTGATGCTATTCGTGCGCGCCGGGCATTGGATGCAACAAAAGAAGCACTAGACGCTGTATTACAA
TTATAA

Upstream 100 bases:

>100_bases
ACGAAGCATGGCCAGAAATTTCTCCACGCTAAACACCTACTCGCTTATGTGAAAATAATAACGTAGTAGGAAGACTCCCT
GTGAAAGGAATGTGTTATTT

Downstream 100 bases:

>100_bases
TTTATCATTCTACTCGCTGTAATGTGCAGCGAGTAGGATTCATTCGGAGGAAAACAAAATGGAAAATAGAATTTCAGGAA
CGACTAGGCTTCTTGGCTTA

Product: NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MKFNSMFSPIDIGPMKVPNRFVVSPMCNNYANTDGTLSDTSLAYYKERALGGFGFITFEATVVDVRAKGGANKACLYSDH
QIASFKRVIDVCHDAGAKISVQLQHAGPEGNSKVSGYPLRAASAIASAAGRNTPEAISREELYELIELYGEAALRAKKAG
ADAVEVHCAHGYLVSSFLSARTNKRVDEFGGCFENRMRLPRLIIESIRKRVGHSIAILCRINSTDGVEGGLSVQDSATVA
AYLEDCGLDGLHVSRSVHIRDEYMWAPTVLHAGFSSDLVTQIKRAVSIPVITVGRFTEPHYAELMVREGRADLVAFGRQS
LADPETPNKAAAGKLDELLPCIACLQGCVANMYAGKPITCLVNPLLGRESEAYSPKTPSKKVVVIGGGVGGLYAGWMAGS
RGHDVTVYEASDMIGGQMRLAAYPPGKGDLTNMVRSYIKKCEQFDVEIKTNTPVTPELIQEVAPDAVIIATGATPLVLPI
PGIEDAGLIHAVDLLDGKEKCGQKVLVVGGGMVGSETAAFLGEAGHDVTVVELRDEVGADVISEHRKFLMRDFDEYKIKS
VTNAKVTSFFEDGVTYSLADNKEHRIDGFDSVVLAMGSRAYNPLEEAIKKMVPETYVIGDAIRARRALDATKEALDAVLQ
L

Sequences:

>Translated_641_residues
MKFNSMFSPIDIGPMKVPNRFVVSPMCNNYANTDGTLSDTSLAYYKERALGGFGFITFEATVVDVRAKGGANKACLYSDH
QIASFKRVIDVCHDAGAKISVQLQHAGPEGNSKVSGYPLRAASAIASAAGRNTPEAISREELYELIELYGEAALRAKKAG
ADAVEVHCAHGYLVSSFLSARTNKRVDEFGGCFENRMRLPRLIIESIRKRVGHSIAILCRINSTDGVEGGLSVQDSATVA
AYLEDCGLDGLHVSRSVHIRDEYMWAPTVLHAGFSSDLVTQIKRAVSIPVITVGRFTEPHYAELMVREGRADLVAFGRQS
LADPETPNKAAAGKLDELLPCIACLQGCVANMYAGKPITCLVNPLLGRESEAYSPKTPSKKVVVIGGGVGGLYAGWMAGS
RGHDVTVYEASDMIGGQMRLAAYPPGKGDLTNMVRSYIKKCEQFDVEIKTNTPVTPELIQEVAPDAVIIATGATPLVLPI
PGIEDAGLIHAVDLLDGKEKCGQKVLVVGGGMVGSETAAFLGEAGHDVTVVELRDEVGADVISEHRKFLMRDFDEYKIKS
VTNAKVTSFFEDGVTYSLADNKEHRIDGFDSVVLAMGSRAYNPLEEAIKKMVPETYVIGDAIRARRALDATKEALDAVLQ
L
>Mature_641_residues
MKFNSMFSPIDIGPMKVPNRFVVSPMCNNYANTDGTLSDTSLAYYKERALGGFGFITFEATVVDVRAKGGANKACLYSDH
QIASFKRVIDVCHDAGAKISVQLQHAGPEGNSKVSGYPLRAASAIASAAGRNTPEAISREELYELIELYGEAALRAKKAG
ADAVEVHCAHGYLVSSFLSARTNKRVDEFGGCFENRMRLPRLIIESIRKRVGHSIAILCRINSTDGVEGGLSVQDSATVA
AYLEDCGLDGLHVSRSVHIRDEYMWAPTVLHAGFSSDLVTQIKRAVSIPVITVGRFTEPHYAELMVREGRADLVAFGRQS
LADPETPNKAAAGKLDELLPCIACLQGCVANMYAGKPITCLVNPLLGRESEAYSPKTPSKKVVVIGGGVGGLYAGWMAGS
RGHDVTVYEASDMIGGQMRLAAYPPGKGDLTNMVRSYIKKCEQFDVEIKTNTPVTPELIQEVAPDAVIIATGATPLVLPI
PGIEDAGLIHAVDLLDGKEKCGQKVLVVGGGMVGSETAAFLGEAGHDVTVVELRDEVGADVISEHRKFLMRDFDEYKIKS
VTNAKVTSFFEDGVTYSLADNKEHRIDGFDSVVLAMGSRAYNPLEEAIKKMVPETYVIGDAIRARRALDATKEALDAVLQ
L

Specific function: Catalyzes The NADP-Dependent Reduction Of 2,4-Dienoyl- CoA To Yield Trans-2- Enoyl-CoA. [C]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=532, Percent_Identity=32.8947368421053, Blast_Score=281, Evalue=1e-76,
Organism=Escherichia coli, GI1787939, Length=347, Percent_Identity=28.5302593659942, Blast_Score=127, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17559804, Length=337, Percent_Identity=27.5964391691395, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17564188, Length=315, Percent_Identity=27.3015873015873, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17559802, Length=336, Percent_Identity=26.7857142857143, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI72001454, Length=342, Percent_Identity=26.6081871345029, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17565138, Length=256, Percent_Identity=27.34375, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=345, Percent_Identity=25.5072463768116, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=352, Percent_Identity=24.1477272727273, Blast_Score=84, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325086, Length=367, Percent_Identity=25.8855585831063, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321973, Length=355, Percent_Identity=25.3521126760563, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 68969; Mature: 68969

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00383 TYR_PHOSPHATASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFNSMFSPIDIGPMKVPNRFVVSPMCNNYANTDGTLSDTSLAYYKERALGGFGFITFEA
CCCCCCCCCCCCCCCCCCCEEEECCHHCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEE
TVVDVRAKGGANKACLYSDHQIASFKRVIDVCHDAGAKISVQLQHAGPEGNSKVSGYPLR
EEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCH
AASAIASAAGRNTPEAISREELYELIELYGEAALRAKKAGADAVEVHCAHGYLVSSFLSA
HHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHH
RTNKRVDEFGGCFENRMRLPRLIIESIRKRVGHSIAILCRINSTDGVEGGLSVQDSATVA
HHCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHH
AYLEDCGLDGLHVSRSVHIRDEYMWAPTVLHAGFSSDLVTQIKRAVSIPVITVGRFTEPH
HHHHHCCCCCEEECEEEEEECCEEECHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCH
YAELMVREGRADLVAFGRQSLADPETPNKAAAGKLDELLPCIACLQGCVANMYAGKPITC
HHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHE
LVNPLLGRESEAYSPKTPSKKVVVIGGGVGGLYAGWMAGSRGHDVTVYEASDMIGGQMRL
EECHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEE
AAYPPGKGDLTNMVRSYIKKCEQFDVEIKTNTPVTPELIQEVAPDAVIIATGATPLVLPI
EECCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHCCCEEEEEECCCEEEEEC
PGIEDAGLIHAVDLLDGKEKCGQKVLVVGGGMVGSETAAFLGEAGHDVTVVELRDEVGAD
CCCCCCCCEEEEHHCCCHHHCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEECHHHCHH
VISEHRKFLMRDFDEYKIKSVTNAKVTSFFEDGVTYSLADNKEHRIDGFDSVVLAMGSRA
HHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCCC
YNPLEEAIKKMVPETYVIGDAIRARRALDATKEALDAVLQL
CCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFNSMFSPIDIGPMKVPNRFVVSPMCNNYANTDGTLSDTSLAYYKERALGGFGFITFEA
CCCCCCCCCCCCCCCCCCCEEEECCHHCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEE
TVVDVRAKGGANKACLYSDHQIASFKRVIDVCHDAGAKISVQLQHAGPEGNSKVSGYPLR
EEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCH
AASAIASAAGRNTPEAISREELYELIELYGEAALRAKKAGADAVEVHCAHGYLVSSFLSA
HHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHH
RTNKRVDEFGGCFENRMRLPRLIIESIRKRVGHSIAILCRINSTDGVEGGLSVQDSATVA
HHCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHH
AYLEDCGLDGLHVSRSVHIRDEYMWAPTVLHAGFSSDLVTQIKRAVSIPVITVGRFTEPH
HHHHHCCCCCEEECEEEEEECCEEECHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCH
YAELMVREGRADLVAFGRQSLADPETPNKAAAGKLDELLPCIACLQGCVANMYAGKPITC
HHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHE
LVNPLLGRESEAYSPKTPSKKVVVIGGGVGGLYAGWMAGSRGHDVTVYEASDMIGGQMRL
EECHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEE
AAYPPGKGDLTNMVRSYIKKCEQFDVEIKTNTPVTPELIQEVAPDAVIIATGATPLVLPI
EECCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHCCCEEEEEECCCEEEEEC
PGIEDAGLIHAVDLLDGKEKCGQKVLVVGGGMVGSETAAFLGEAGHDVTVVELRDEVGAD
CCCCCCCCEEEEHHCCCHHHCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEECHHHCHH
VISEHRKFLMRDFDEYKIKSVTNAKVTSFFEDGVTYSLADNKEHRIDGFDSVVLAMGSRA
HHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCCC
YNPLEEAIKKMVPETYVIGDAIRARRALDATKEALDAVLQL
CCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491719 [H]