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The map label for this gene is leuD

Identifier: 226224588

GI number: 226224588

Start: 2052378

End: 2052959

Strand: Direct

Name: leuD

Synonym: Lm4b_02002

Alternate gene names: 226224588

Gene position: 2052378-2052959 (Clockwise)

Preceding gene: 226224587

Following gene: 226224589

Centisome position: 70.46

GC content: 40.38

Gene sequence:

>582_bases
GTGGAGGAAATTAAAGTACACATTGGAAAAACAGTAGCGCTAATGAATGACAATATTGATACCGACCAAATCATTCCAAA
AAGTTTCTTAAAACGCATTGAGCGAACAGGCTTTGGCGAATTTCTCTTTGATAGTTGGCGCTACCTGCCGAACCGTAAAC
CAAACCCAGACTTCCCGCTCAATGCTCCAGATCGTCAGGAAGCGACTATTTTAATTACGGGAGATAACTTTGGTTGCGGG
TCCTCCAGAGAACATGCTGCGTGGGCACTACTCGACTACCGTTTCCGTGTAATTATCGCTGGCAGTTATAGTGATATATT
TTATATGAATTGTACAAAAAACGGCGTGCTCCCTATCGTTCTTCCTAGAGAGGCGCGTGAAAAATTAGCGAAAATTGCAG
CCGATGAAAATGTGACCATTGACTTGCCAAATCAACAAGTTATCAGTTCAGTTGGTACCTACCCGTTTGAGATTGATGCT
ACTTGGAAAAATAAATTTATTAATGGACTGGATGATATCGCCATTACATTTGAACATATTGATGCGATTAAAGCCTATGA
ACAAAAAGTGGATTCAATATAA

Upstream 100 bases:

>100_bases
CAAGGTAAAGGCGCTCGTACTCATCTTGTTTCACCAGCAATGGCTGCTGCTGCGGCAATTAACGGGCACTTTATCGATAT
TCGAAAGGAGGCGGTTATCA

Downstream 100 bases:

>100_bases
GAAAAGGTAGTGAGGATGATGGAATTAGTTGATTTATTAGTAACAGAAGAAGATGTCGAAAAGGCCTATGAAGTCTTAAA
GGCGGTCGTCAAACATACGC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MEEIKVHIGKTVALMNDNIDTDQIIPKSFLKRIERTGFGEFLFDSWRYLPNRKPNPDFPLNAPDRQEATILITGDNFGCG
SSREHAAWALLDYRFRVIIAGSYSDIFYMNCTKNGVLPIVLPREAREKLAKIAADENVTIDLPNQQVISSVGTYPFEIDA
TWKNKFINGLDDIAITFEHIDAIKAYEQKVDSI

Sequences:

>Translated_193_residues
MEEIKVHIGKTVALMNDNIDTDQIIPKSFLKRIERTGFGEFLFDSWRYLPNRKPNPDFPLNAPDRQEATILITGDNFGCG
SSREHAAWALLDYRFRVIIAGSYSDIFYMNCTKNGVLPIVLPREAREKLAKIAADENVTIDLPNQQVISSVGTYPFEIDA
TWKNKFINGLDDIAITFEHIDAIKAYEQKVDSI
>Mature_193_residues
MEEIKVHIGKTVALMNDNIDTDQIIPKSFLKRIERTGFGEFLFDSWRYLPNRKPNPDFPLNAPDRQEATILITGDNFGCG
SSREHAAWALLDYRFRVIIAGSYSDIFYMNCTKNGVLPIVLPREAREKLAKIAADENVTIDLPNQQVISSVGTYPFEIDA
TWKNKFINGLDDIAITFEHIDAIKAYEQKVDSI

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=193, Percent_Identity=49.2227979274611, Blast_Score=181, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6321429, Length=188, Percent_Identity=47.8723404255319, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 21942; Mature: 21942

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEIKVHIGKTVALMNDNIDTDQIIPKSFLKRIERTGFGEFLFDSWRYLPNRKPNPDFPL
CCCEEEEECCEEEEECCCCCCHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCC
NAPDRQEATILITGDNFGCGSSREHAAWALLDYRFRVIIAGSYSDIFYMNCTKNGVLPIV
CCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEEEEEEEECCCCEEEEEECCCCEEEEE
LPREAREKLAKIAADENVTIDLPNQQVISSVGTYPFEIDATWKNKFINGLDDIAITFEHI
ECHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCEEEECCHHHHHHCCCHHHEEHHHHH
DAIKAYEQKVDSI
HHHHHHHHHHCCC
>Mature Secondary Structure
MEEIKVHIGKTVALMNDNIDTDQIIPKSFLKRIERTGFGEFLFDSWRYLPNRKPNPDFPL
CCCEEEEECCEEEEECCCCCCHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCC
NAPDRQEATILITGDNFGCGSSREHAAWALLDYRFRVIIAGSYSDIFYMNCTKNGVLPIV
CCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEEEEEEEECCCCEEEEEECCCCEEEEE
LPREAREKLAKIAADENVTIDLPNQQVISSVGTYPFEIDATWKNKFINGLDDIAITFEHI
ECHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCEEEECCHHHHHHCCCHHHEEHHHHH
DAIKAYEQKVDSI
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]