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The map label for this gene is leuC

Identifier: 226224587

GI number: 226224587

Start: 2051003

End: 2052391

Strand: Direct

Name: leuC

Synonym: Lm4b_02001

Alternate gene names: 226224587

Gene position: 2051003-2052391 (Clockwise)

Preceding gene: 226224586

Following gene: 226224588

Centisome position: 70.42

GC content: 42.91

Gene sequence:

>1389_bases
ATGGGGAAAACACTTTTTGATAAACTTTGGAATCGCCATGTCATTTATGGCAAAGAAGGTGAACCGCAATTATTATACGT
TGACCTTCATTTGATTCATGAAGTTACTTCTCCTCAAGCGTTTGAAGGACTTAGAATGGAAAATCGGCCACTGCGTAGAC
CAGATAAAACGTTCGCGACAATGGATCATAACGTTCCAACTGAAGATATTTTCAACATTCAAGACCTTGTTGCTAAAAAA
CAAATCGAAGCACTGCAAACCAATTGCGAGGAATTTGGCGTGACGCTAGCTGATATGGGTAGTGATCGCCAAGGAATCGT
GCATATGGTTGGACCAGAAACTGGGCTAACACAACCTGGAAAAGTCATTGTATGTGGAGATTCGCATACTGCAACTCATG
GAGCATTTGGAGCAATTGGCTTTGGGATTGGTTCTAGTGAAGTAGAGCATGTTTTTGCGACTCAAACCATTTGGCAACAA
AAGCCAAAATCAATGGGCATAGAAATTAACGGCAATCTTCCAAAAGGTGTCTATGCAAAAGATATTATTTTGCATCTGAT
TGCCACGTATGGCGTCGCTTTTGGAACTGGCTATGCCGTTGAGTATTACGGCGAAACTATTCGCAATATGTCGATGGAAG
AACGGATGACGATTTGTAATATGGCAATTGAAGGTGGCGCAAAGATGGGCATGATGGCGCCAGATGAAACTACTTTTGAA
TATGTGCGCGGTCGTGAATACGCTCCCGCTGATATGGATAAAGCAATTAGTGACTGGAAAACGCTCCAAACAGATCCAGA
TGCCGAATATGACCTTCATATCAAAATGGATGCGAGCATTTTGGAACCGTATGTCACTTGGGGAACGAATCCCGAAATGG
GAGTGCCATTTTCTAAAGCATTTCCGGAAATTAAAGATATGAACTATGAGCGTGCTTATGAATATATGGGGCTAAAACCT
GGTCAAACTGCAGAAGAAATCGAGCTTGGCTATGTGTTTATTGGTTCTTGTACAAATGCCAGACTTTCTGACTTAGAAGA
AGCTGCTCGTATCGTCAAAGGAAATAAAGTGAAAAATAATATTCGCGCACTCGTGGTCCCTGGTTCTCGCCAAGTGCGTA
ATGCGGCAGAATCTATCGGACTAGATAAAATTTTTATAGAAGCTGGCTTTGAGTGGCGTGAACCTGGTTGTTCGATGTGT
CTAGGAATGAACCCTGATCAAGTACCAGACGGCGTTCACTGTGCTTCTACTTCGAACCGAAATTTTGAAGGTCGTCAAGG
TAAAGGCGCTCGTACTCATCTTGTTTCACCAGCAATGGCTGCTGCTGCGGCAATTAACGGGCACTTTATCGATATTCGAA
AGGAGGCGGTTATCAGTGGAGGAAATTAA

Upstream 100 bases:

>100_bases
ACGATGCAAGCTGGATTTTTAACTGCTGACCTTGGAGGCAATACTTCCACAACTGATTTTACAAATGAAGTTCTAAAACA
AATTGAAGGAGGAGAATAAA

Downstream 100 bases:

>100_bases
AGTACACATTGGAAAAACAGTAGCGCTAATGAATGACAATATTGATACCGACCAAATCATTCCAAAAAGTTTCTTAAAAC
GCATTGAGCGAACAGGCTTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MGKTLFDKLWNRHVIYGKEGEPQLLYVDLHLIHEVTSPQAFEGLRMENRPLRRPDKTFATMDHNVPTEDIFNIQDLVAKK
QIEALQTNCEEFGVTLADMGSDRQGIVHMVGPETGLTQPGKVIVCGDSHTATHGAFGAIGFGIGSSEVEHVFATQTIWQQ
KPKSMGIEINGNLPKGVYAKDIILHLIATYGVAFGTGYAVEYYGETIRNMSMEERMTICNMAIEGGAKMGMMAPDETTFE
YVRGREYAPADMDKAISDWKTLQTDPDAEYDLHIKMDASILEPYVTWGTNPEMGVPFSKAFPEIKDMNYERAYEYMGLKP
GQTAEEIELGYVFIGSCTNARLSDLEEAARIVKGNKVKNNIRALVVPGSRQVRNAAESIGLDKIFIEAGFEWREPGCSMC
LGMNPDQVPDGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAINGHFIDIRKEAVISGGN

Sequences:

>Translated_462_residues
MGKTLFDKLWNRHVIYGKEGEPQLLYVDLHLIHEVTSPQAFEGLRMENRPLRRPDKTFATMDHNVPTEDIFNIQDLVAKK
QIEALQTNCEEFGVTLADMGSDRQGIVHMVGPETGLTQPGKVIVCGDSHTATHGAFGAIGFGIGSSEVEHVFATQTIWQQ
KPKSMGIEINGNLPKGVYAKDIILHLIATYGVAFGTGYAVEYYGETIRNMSMEERMTICNMAIEGGAKMGMMAPDETTFE
YVRGREYAPADMDKAISDWKTLQTDPDAEYDLHIKMDASILEPYVTWGTNPEMGVPFSKAFPEIKDMNYERAYEYMGLKP
GQTAEEIELGYVFIGSCTNARLSDLEEAARIVKGNKVKNNIRALVVPGSRQVRNAAESIGLDKIFIEAGFEWREPGCSMC
LGMNPDQVPDGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAINGHFIDIRKEAVISGGN
>Mature_461_residues
GKTLFDKLWNRHVIYGKEGEPQLLYVDLHLIHEVTSPQAFEGLRMENRPLRRPDKTFATMDHNVPTEDIFNIQDLVAKKQ
IEALQTNCEEFGVTLADMGSDRQGIVHMVGPETGLTQPGKVIVCGDSHTATHGAFGAIGFGIGSSEVEHVFATQTIWQQK
PKSMGIEINGNLPKGVYAKDIILHLIATYGVAFGTGYAVEYYGETIRNMSMEERMTICNMAIEGGAKMGMMAPDETTFEY
VRGREYAPADMDKAISDWKTLQTDPDAEYDLHIKMDASILEPYVTWGTNPEMGVPFSKAFPEIKDMNYERAYEYMGLKPG
QTAEEIELGYVFIGSCTNARLSDLEEAARIVKGNKVKNNIRALVVPGSRQVRNAAESIGLDKIFIEAGFEWREPGCSMCL
GMNPDQVPDGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAINGHFIDIRKEAVISGGN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=379, Percent_Identity=28.4960422163588, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI41352693, Length=382, Percent_Identity=27.4869109947644, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI8659555, Length=378, Percent_Identity=25.6613756613757, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=58.8362068965517, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1787531, Length=412, Percent_Identity=25.2427184466019, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081781, Length=360, Percent_Identity=23.8888888888889, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI2367097, Length=344, Percent_Identity=26.1627906976744, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI32564738, Length=394, Percent_Identity=30.4568527918782, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI25149337, Length=394, Percent_Identity=30.4568527918782, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=304, Percent_Identity=30.9210526315789, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=453, Percent_Identity=27.1523178807947, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=469, Percent_Identity=53.9445628997868, Blast_Score=496, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6320440, Length=412, Percent_Identity=25.9708737864078, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6322261, Length=376, Percent_Identity=30.5851063829787, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6323335, Length=358, Percent_Identity=30.7262569832402, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI281365315, Length=391, Percent_Identity=28.6445012787724, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI17864292, Length=391, Percent_Identity=28.6445012787724, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI161076999, Length=391, Percent_Identity=28.6445012787724, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI28571643, Length=391, Percent_Identity=27.8772378516624, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI17137564, Length=463, Percent_Identity=25.4859611231102, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24645686, Length=462, Percent_Identity=26.1904761904762, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50943; Mature: 50811

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTLFDKLWNRHVIYGKEGEPQLLYVDLHLIHEVTSPQAFEGLRMENRPLRRPDKTFAT
CCCHHHHHHCCCEEEECCCCCCCEEEEEHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHEE
MDHNVPTEDIFNIQDLVAKKQIEALQTNCEEFGVTLADMGSDRQGIVHMVGPETGLTQPG
CCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCC
KVIVCGDSHTATHGAFGAIGFGIGSSEVEHVFATQTIWQQKPKSMGIEINGNLPKGVYAK
EEEEECCCCCCCCCCCHHEEECCCCHHHHHHHHHHHHHHCCCHHCCEEECCCCCCCHHHH
DIILHLIATYGVAFGTGYAVEYYGETIRNMSMEERMTICNMAIEGGAKMGMMAPDETTFE
HHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
YVRGREYAPADMDKAISDWKTLQTDPDAEYDLHIKMDASILEPYVTWGTNPEMGVPFSKA
HHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHCHHHCCCCCCCCCCCHHHH
FPEIKDMNYERAYEYMGLKPGQTAEEIELGYVFIGSCTNARLSDLEEAARIVKGNKVKNN
CCHHHCCCHHHHHHHCCCCCCCCHHHEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
IRALVVPGSRQVRNAAESIGLDKIFIEAGFEWREPGCSMCLGMNPDQVPDGVHCASTSNR
CEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCEEECCCCCC
NFEGRQGKGARTHLVSPAMAAAAAINGHFIDIRKEAVISGGN
CCCCCCCCCCCHHHCCHHHHHHHHHCCEEEEEEHEEEECCCC
>Mature Secondary Structure 
GKTLFDKLWNRHVIYGKEGEPQLLYVDLHLIHEVTSPQAFEGLRMENRPLRRPDKTFAT
CCHHHHHHCCCEEEECCCCCCCEEEEEHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHEE
MDHNVPTEDIFNIQDLVAKKQIEALQTNCEEFGVTLADMGSDRQGIVHMVGPETGLTQPG
CCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCC
KVIVCGDSHTATHGAFGAIGFGIGSSEVEHVFATQTIWQQKPKSMGIEINGNLPKGVYAK
EEEEECCCCCCCCCCCHHEEECCCCHHHHHHHHHHHHHHCCCHHCCEEECCCCCCCHHHH
DIILHLIATYGVAFGTGYAVEYYGETIRNMSMEERMTICNMAIEGGAKMGMMAPDETTFE
HHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
YVRGREYAPADMDKAISDWKTLQTDPDAEYDLHIKMDASILEPYVTWGTNPEMGVPFSKA
HHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHCHHHCCCCCCCCCCCHHHH
FPEIKDMNYERAYEYMGLKPGQTAEEIELGYVFIGSCTNARLSDLEEAARIVKGNKVKNN
CCHHHCCCHHHHHHHCCCCCCCCHHHEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
IRALVVPGSRQVRNAAESIGLDKIFIEAGFEWREPGCSMCLGMNPDQVPDGVHCASTSNR
CEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCEEECCCCCC
NFEGRQGKGARTHLVSPAMAAAAAINGHFIDIRKEAVISGGN
CCCCCCCCCCCHHHCCHHHHHHHHHCCEEEEEEHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]