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The map label for this gene is ilvA

Identifier: 226224589

GI number: 226224589

Start: 2052978

End: 2054246

Strand: Direct

Name: ilvA

Synonym: Lm4b_02003

Alternate gene names: 226224589

Gene position: 2052978-2054246 (Clockwise)

Preceding gene: 226224588

Following gene: 226224590

Centisome position: 70.48

GC content: 40.74

Gene sequence:

>1269_bases
ATGGAATTAGTTGATTTATTAGTAACAGAAGAAGATGTCGAAAAGGCCTATGAAGTCTTAAAGGCGGTCGTCAAACATAC
GCCACTAGAATATGATTTTTATCTTTCGGAAAAATATCATTGCAATGTCTATCTGAAACGCGAAGATTTGCAAAGAGTGC
GTTCTTTTAAATTACGTGGTGCTTTTTATGCTATTTCTAGATTATCAGCGGAACAATTGGAAAAAGGTGTGGTATGTGCA
AGTGCGGGAAACCATGCGCAAGGAGTTGCATATACGTGCAAACGAATGACCGTCCCAGCTACGATTTTTATGCCAACAAC
TACCCCGCAACAAAAAGTATCACAAGTAAAATTTTTCGGTGGTAGTAATGTCGAAGTGGTTTTAGTTGGCGATACGTTTG
ATGCTTCTGCAACTGCCGCGAAAGAATTTGCTGCAACACATGGCCAAACTTTCATCCCTCCGTTTGATGATCCTGATATT
ATCGCTGGCCAAGGCTCTCTTGCTGTGGAGATGGTGAAAGATTTAAATAAAGCACATGAGCAAGCAGATTATGTTTTTGC
AGGAATCGGTGGTGGTGGCTTAATTAGCGGTGTTGCAACGTATCTCAAAGCAAAGAGCCCGATTACAAAAATCATCGGGG
TAGAACCTGATGGCGCGCCTTCAATGACAGCAGCTTTAAAACAAAATCAAGTCGTAACACTAGATAAAATCGATAAATTT
GTTGATGGCGCTGCGGTAAAAGAAGTTGGTGGCTTAACGTTCCAACATGCGAAAGTATTAGTTGATGAAGTGACAACCGT
CTCTGAGGGAGCTGTTTGTTCTACGATTTTGGATATGTATACGAAGCAAGCAATTGTTGCAGAACCTGCTGGAGCACTAT
CCGTTGCCGCACTTGAAATTTACCGTGAAGAGATTAAAGACAAGACCGTTGTCTGTATTGTAAGTGGCGGAAACAACGAC
ATCAACCGGATGCAAGAAATTGAAGAGCGTTCCCTCTTACATGAAGGATTAAAACATTATTTTATCGTCAATTTCTCGCA
GCGTCCTGGGGCTTTGAAAGAATTTGTTAACGATGTGCTCGGTCCACATGACGATATCACCAAATTCGAATATACGAAAA
AAGTGAATCGAGGTAATGGGCCTGTAATTATCGGTGTTTTGTTGCAAGATAAAAATGATTATGAAGGATTGCTTGAGCGG
GTTGCTGCTTTTGACCCGAGCTATATTCCGATTAATGATAATCAAACCCTATACACATTACTTGTATAA

Upstream 100 bases:

>100_bases
TAATGGACTGGATGATATCGCCATTACATTTGAACATATTGATGCGATTAAAGCCTATGAACAAAAAGTGGATTCAATAT
AAGAAAAGGTAGTGAGGATG

Downstream 100 bases:

>100_bases
AAAGAGTTCTCGTATGATGCGAGAACTCTTTTTATATTACAGTTTACTATCCTGTTATAGTAACAGTTTATTTTAAAATT
TGGTTTAAAATCTTTTGTTT

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MELVDLLVTEEDVEKAYEVLKAVVKHTPLEYDFYLSEKYHCNVYLKREDLQRVRSFKLRGAFYAISRLSAEQLEKGVVCA
SAGNHAQGVAYTCKRMTVPATIFMPTTTPQQKVSQVKFFGGSNVEVVLVGDTFDASATAAKEFAATHGQTFIPPFDDPDI
IAGQGSLAVEMVKDLNKAHEQADYVFAGIGGGGLISGVATYLKAKSPITKIIGVEPDGAPSMTAALKQNQVVTLDKIDKF
VDGAAVKEVGGLTFQHAKVLVDEVTTVSEGAVCSTILDMYTKQAIVAEPAGALSVAALEIYREEIKDKTVVCIVSGGNND
INRMQEIEERSLLHEGLKHYFIVNFSQRPGALKEFVNDVLGPHDDITKFEYTKKVNRGNGPVIIGVLLQDKNDYEGLLER
VAAFDPSYIPINDNQTLYTLLV

Sequences:

>Translated_422_residues
MELVDLLVTEEDVEKAYEVLKAVVKHTPLEYDFYLSEKYHCNVYLKREDLQRVRSFKLRGAFYAISRLSAEQLEKGVVCA
SAGNHAQGVAYTCKRMTVPATIFMPTTTPQQKVSQVKFFGGSNVEVVLVGDTFDASATAAKEFAATHGQTFIPPFDDPDI
IAGQGSLAVEMVKDLNKAHEQADYVFAGIGGGGLISGVATYLKAKSPITKIIGVEPDGAPSMTAALKQNQVVTLDKIDKF
VDGAAVKEVGGLTFQHAKVLVDEVTTVSEGAVCSTILDMYTKQAIVAEPAGALSVAALEIYREEIKDKTVVCIVSGGNND
INRMQEIEERSLLHEGLKHYFIVNFSQRPGALKEFVNDVLGPHDDITKFEYTKKVNRGNGPVIIGVLLQDKNDYEGLLER
VAAFDPSYIPINDNQTLYTLLV
>Mature_422_residues
MELVDLLVTEEDVEKAYEVLKAVVKHTPLEYDFYLSEKYHCNVYLKREDLQRVRSFKLRGAFYAISRLSAEQLEKGVVCA
SAGNHAQGVAYTCKRMTVPATIFMPTTTPQQKVSQVKFFGGSNVEVVLVGDTFDASATAAKEFAATHGQTFIPPFDDPDI
IAGQGSLAVEMVKDLNKAHEQADYVFAGIGGGGLISGVATYLKAKSPITKIIGVEPDGAPSMTAALKQNQVVTLDKIDKF
VDGAAVKEVGGLTFQHAKVLVDEVTTVSEGAVCSTILDMYTKQAIVAEPAGALSVAALEIYREEIKDKTVVCIVSGGNND
INRMQEIEERSLLHEGLKHYFIVNFSQRPGALKEFVNDVLGPHDDITKFEYTKKVNRGNGPVIIGVLLQDKNDYEGLLER
VAAFDPSYIPINDNQTLYTLLV

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=323, Percent_Identity=31.2693498452012, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI19923959, Length=311, Percent_Identity=28.6173633440514, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI33469958, Length=321, Percent_Identity=30.2180685358255, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1790207, Length=396, Percent_Identity=37.3737373737374, Blast_Score=207, Evalue=7e-55,
Organism=Escherichia coli, GI1789505, Length=325, Percent_Identity=36, Blast_Score=179, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI71991565, Length=354, Percent_Identity=36.7231638418079, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17537387, Length=345, Percent_Identity=30.4347826086957, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17508781, Length=318, Percent_Identity=26.4150943396226, Blast_Score=105, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320930, Length=361, Percent_Identity=38.781163434903, Blast_Score=230, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6322631, Length=318, Percent_Identity=34.9056603773585, Blast_Score=173, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6319788, Length=288, Percent_Identity=28.125, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21355833, Length=349, Percent_Identity=32.9512893982808, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24645328, Length=253, Percent_Identity=31.2252964426877, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR001721
- InterPro:   IPR011820 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 46093; Mature: 46093

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELVDLLVTEEDVEKAYEVLKAVVKHTPLEYDFYLSEKYHCNVYLKREDLQRVRSFKLRG
CCHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEEEHHHHHHHHHHHHHH
AFYAISRLSAEQLEKGVVCASAGNHAQGVAYTCKRMTVPATIFMPTTTPQQKVSQVKFFG
HHHHHHHCCHHHHHCCEEEECCCCCCCCHHEEHHEEECCEEEEECCCCHHHHHHHEEEEC
GSNVEVVLVGDTFDASATAAKEFAATHGQTFIPPFDDPDIIAGQGSLAVEMVKDLNKAHE
CCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHH
QADYVFAGIGGGGLISGVATYLKAKSPITKIIGVEPDGAPSMTAALKQNQVVTLDKIDKF
HCCEEEEECCCCHHHHHHHHHHHCCCCHHHEEECCCCCCCHHHHHHCCCCEEEHHHHHHH
VDGAAVKEVGGLTFQHAKVLVDEVTTVSEGAVCSTILDMYTKQAIVAEPAGALSVAALEI
HCCHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHH
YREEIKDKTVVCIVSGGNNDINRMQEIEERSLLHEGLKHYFIVNFSQRPGALKEFVNDVL
HHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHC
GPHDDITKFEYTKKVNRGNGPVIIGVLLQDKNDYEGLLERVAAFDPSYIPINDNQTLYTL
CCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCEEEEE
LV
EC
>Mature Secondary Structure
MELVDLLVTEEDVEKAYEVLKAVVKHTPLEYDFYLSEKYHCNVYLKREDLQRVRSFKLRG
CCHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEEEHHHHHHHHHHHHHH
AFYAISRLSAEQLEKGVVCASAGNHAQGVAYTCKRMTVPATIFMPTTTPQQKVSQVKFFG
HHHHHHHCCHHHHHCCEEEECCCCCCCCHHEEHHEEECCEEEEECCCCHHHHHHHEEEEC
GSNVEVVLVGDTFDASATAAKEFAATHGQTFIPPFDDPDIIAGQGSLAVEMVKDLNKAHE
CCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHH
QADYVFAGIGGGGLISGVATYLKAKSPITKIIGVEPDGAPSMTAALKQNQVVTLDKIDKF
HCCEEEEECCCCHHHHHHHHHHHCCCCHHHEEECCCCCCCHHHHHHCCCCEEEHHHHHHH
VDGAAVKEVGGLTFQHAKVLVDEVTTVSEGAVCSTILDMYTKQAIVAEPAGALSVAALEI
HCCHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHH
YREEIKDKTVVCIVSGGNNDINRMQEIEERSLLHEGLKHYFIVNFSQRPGALKEFVNDVL
HHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHC
GPHDDITKFEYTKKVNRGNGPVIIGVLLQDKNDYEGLLERVAAFDPSYIPINDNQTLYTL
CCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCEEEEE
LV
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA