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The map label for this gene is leuB

Identifier: 226224586

GI number: 226224586

Start: 2049949

End: 2051001

Strand: Direct

Name: leuB

Synonym: Lm4b_02000

Alternate gene names: 226224586

Gene position: 2049949-2051001 (Clockwise)

Preceding gene: 226224585

Following gene: 226224587

Centisome position: 70.38

GC content: 43.02

Gene sequence:

>1053_bases
GTGACCTATAAAATTACTTCCTTAGCTGGCGACGGAATTGGTCCAGAAATTATGACTTCTGGACTTCAAGTATTAGAGGC
AGTCGAAAAAAAATACAACCATACGTTTGAAATTGAATCTCATCCCTTTGGCGGCGCAGGAATTGACGTAGCACAAGACC
CAATCCCTTCTTCCACCTTAAAAGCGTGCCGAGACGCCGACGCCATTTTACTCGGAGCAATTGGTGGTCCAAAATGGGAT
AATGCGCCGAAACGTCCCGAAGATGGCCTACTTGCACTTCGAAAAGCGCTTGGTCTTTTCGCCAACATTCGACCTATCCA
AGTCCCAAGCTCCATCACCCACCTTTCTCCTTTAAAAAAGGAAATTGTTGAGAACACTGATTTTGTCGTTGTTCGTGAAT
TGACTGGTGGACTTTATTTCGGAGAACCGAAATATTGGGATAATGAGGCAGCAGTGGATTCATTAACTTATACCCGTGCA
GAAATCGAGCGAATTATCGAGAAAGCTTTCGAAATCGCCGCTACTAGGAATAAAAAAGTTACTTCAGTAGATAAAGCGAA
TGTGCTTGCTTCTAGTAAATTATGGCGAAAAATCGCTGATGAAGTCGCAAGTCGTCATCCAGATATCACACTAGAACATC
TATACGTCGACGCTGCCGCAATGCTTATGATTCAGCGACCAACGACCTTTGATGTTATCGTGACAGAAAACTTATTTGGC
GATATTTTAAGTGATGAAGCGTCTGTTATTACTGGCTCGCTTGGAATGCTCCCTTCTGCTAGCCACGCGGAAAACGGACC
ATCTTTATACGAACCAATTCACGGATCTGCGCCAGATATCGCTAACCAAAATATCGCGAATCCAATGTCGATGATATCCT
CTGTATCCATGATGCTTCGTCAATCTTTCTCTCTTTTTGAAGAAGCAGATGCGATTGATGCAGCTACAGCAAGAACGATG
CAAGCTGGATTTTTAACTGCTGACCTTGGAGGCAATACTTCCACAACTGATTTTACAAATGAAGTTCTAAAACAAATTGA
AGGAGGAGAATAA

Upstream 100 bases:

>100_bases
GATTTTGACGTTTTAACTGCTAGTGCTAAAGCTTATTTGCAAGCATCAGGCAAAAGCAAAACCGCCAGTAAGCAAGCTGA
TTTCGAGGAGGTAAAATAAC

Downstream 100 bases:

>100_bases
AATGGGGAAAACACTTTTTGATAAACTTTGGAATCGCCATGTCATTTATGGCAAAGAAGGTGAACCGCAATTATTATACG
TTGACCTTCATTTGATTCAT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MTYKITSLAGDGIGPEIMTSGLQVLEAVEKKYNHTFEIESHPFGGAGIDVAQDPIPSSTLKACRDADAILLGAIGGPKWD
NAPKRPEDGLLALRKALGLFANIRPIQVPSSITHLSPLKKEIVENTDFVVVRELTGGLYFGEPKYWDNEAAVDSLTYTRA
EIERIIEKAFEIAATRNKKVTSVDKANVLASSKLWRKIADEVASRHPDITLEHLYVDAAAMLMIQRPTTFDVIVTENLFG
DILSDEASVITGSLGMLPSASHAENGPSLYEPIHGSAPDIANQNIANPMSMISSVSMMLRQSFSLFEEADAIDAATARTM
QAGFLTADLGGNTSTTDFTNEVLKQIEGGE

Sequences:

>Translated_350_residues
MTYKITSLAGDGIGPEIMTSGLQVLEAVEKKYNHTFEIESHPFGGAGIDVAQDPIPSSTLKACRDADAILLGAIGGPKWD
NAPKRPEDGLLALRKALGLFANIRPIQVPSSITHLSPLKKEIVENTDFVVVRELTGGLYFGEPKYWDNEAAVDSLTYTRA
EIERIIEKAFEIAATRNKKVTSVDKANVLASSKLWRKIADEVASRHPDITLEHLYVDAAAMLMIQRPTTFDVIVTENLFG
DILSDEASVITGSLGMLPSASHAENGPSLYEPIHGSAPDIANQNIANPMSMISSVSMMLRQSFSLFEEADAIDAATARTM
QAGFLTADLGGNTSTTDFTNEVLKQIEGGE
>Mature_349_residues
TYKITSLAGDGIGPEIMTSGLQVLEAVEKKYNHTFEIESHPFGGAGIDVAQDPIPSSTLKACRDADAILLGAIGGPKWDN
APKRPEDGLLALRKALGLFANIRPIQVPSSITHLSPLKKEIVENTDFVVVRELTGGLYFGEPKYWDNEAAVDSLTYTRAE
IERIIEKAFEIAATRNKKVTSVDKANVLASSKLWRKIADEVASRHPDITLEHLYVDAAAMLMIQRPTTFDVIVTENLFGD
ILSDEASVITGSLGMLPSASHAENGPSLYEPIHGSAPDIANQNIANPMSMISSVSMMLRQSFSLFEEADAIDAATARTMQ
AGFLTADLGGNTSTTDFTNEVLKQIEGGE

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=347, Percent_Identity=36.0230547550432, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI28178821, Length=354, Percent_Identity=28.2485875706215, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI28178816, Length=350, Percent_Identity=28.2857142857143, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI4758582, Length=355, Percent_Identity=30.7042253521127, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI28178838, Length=327, Percent_Identity=31.1926605504587, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178819, Length=192, Percent_Identity=31.7708333333333, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI87081683, Length=354, Percent_Identity=47.7401129943503, Blast_Score=328, Evalue=2e-91,
Organism=Escherichia coli, GI1788101, Length=362, Percent_Identity=32.5966850828729, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1787381, Length=391, Percent_Identity=26.5984654731458, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71986051, Length=358, Percent_Identity=32.4022346368715, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17550882, Length=351, Percent_Identity=29.0598290598291, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=357, Percent_Identity=29.6918767507003, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25144293, Length=357, Percent_Identity=30.5322128851541, Blast_Score=117, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=356, Percent_Identity=46.0674157303371, Blast_Score=296, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6322097, Length=363, Percent_Identity=34.435261707989, Blast_Score=159, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6324709, Length=355, Percent_Identity=35.7746478873239, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6324291, Length=354, Percent_Identity=31.638418079096, Blast_Score=136, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24643268, Length=353, Percent_Identity=35.9773371104816, Blast_Score=177, Evalue=9e-45,
Organism=Drosophila melanogaster, GI24643270, Length=353, Percent_Identity=35.9773371104816, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24661184, Length=354, Percent_Identity=30.225988700565, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI281362242, Length=346, Percent_Identity=29.7687861271676, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648872, Length=346, Percent_Identity=29.7687861271676, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161078637, Length=357, Percent_Identity=27.7310924369748, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI161078635, Length=357, Percent_Identity=27.7310924369748, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI161078633, Length=357, Percent_Identity=27.7310924369748, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24650122, Length=357, Percent_Identity=27.7310924369748, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI161078639, Length=262, Percent_Identity=29.3893129770992, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI20130355, Length=346, Percent_Identity=23.4104046242775, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 37794; Mature: 37663

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYKITSLAGDGIGPEIMTSGLQVLEAVEKKYNHTFEIESHPFGGAGIDVAQDPIPSSTL
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHH
KACRDADAILLGAIGGPKWDNAPKRPEDGLLALRKALGLFANIRPIQVPSSITHLSPLKK
HHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHH
EIVENTDFVVVRELTGGLYFGEPKYWDNEAAVDSLTYTRAEIERIIEKAFEIAATRNKKV
HHHCCCCEEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TSVDKANVLASSKLWRKIADEVASRHPDITLEHLYVDAAAMLMIQRPTTFDVIVTENLFG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCCEEEEEECHHHH
DILSDEASVITGSLGMLPSASHAENGPSLYEPIHGSAPDIANQNIANPMSMISSVSMMLR
HHHCCCHHHEECCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QSFSLFEEADAIDAATARTMQAGFLTADLGGNTSTTDFTNEVLKQIEGGE
HHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TYKITSLAGDGIGPEIMTSGLQVLEAVEKKYNHTFEIESHPFGGAGIDVAQDPIPSSTL
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHH
KACRDADAILLGAIGGPKWDNAPKRPEDGLLALRKALGLFANIRPIQVPSSITHLSPLKK
HHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHH
EIVENTDFVVVRELTGGLYFGEPKYWDNEAAVDSLTYTRAEIERIIEKAFEIAATRNKKV
HHHCCCCEEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TSVDKANVLASSKLWRKIADEVASRHPDITLEHLYVDAAAMLMIQRPTTFDVIVTENLFG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCCEEEEEECHHHH
DILSDEASVITGSLGMLPSASHAENGPSLYEPIHGSAPDIANQNIANPMSMISSVSMMLR
HHHCCCHHHEECCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QSFSLFEEADAIDAATARTMQAGFLTADLGGNTSTTDFTNEVLKQIEGGE
HHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]