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The map label for this gene is leuA

Identifier: 226224585

GI number: 226224585

Start: 2048409

End: 2049947

Strand: Direct

Name: leuA

Synonym: Lm4b_01999

Alternate gene names: 226224585

Gene position: 2048409-2049947 (Clockwise)

Preceding gene: 226224584

Following gene: 226224586

Centisome position: 70.33

GC content: 42.04

Gene sequence:

>1539_bases
ATGAAGAAAATCCAGTTTTTTGATACTACACTTAGAGATGGCGAACAAACCCCTGGAGTTAATTTTGACGTAAAGGAGAA
AATCCAGATTGCCTTACAACTCGAAAAACTTGGAATAGATGTGATTGAAGCCGGCTTTCCGATTTCTTCTCCTGGAGATT
TTGAATGCGTTAAGGCGATTGCAAAAGCGATTAAACATTGTTCTGTAACAGGGCTAGCGCGCTGTGTTGAAGGAGATATT
GACCGTGCCGAGGAAGCGCTAAAAGACGCTGTTTCTCCGCAAATACATATCTTCCTTGCAACGAGCGATGTGCATATGGA
ATACAAATTAAAAATGAGTCGGGCGGAAGTTCTTGCTTCCATTAAACACCACATTAGTTATGCGAGACAAAAATTTGATG
TTGTACAGTTTTCACCAGAAGATGCAACTCGGTCAGATCGTGCTTTTCTGATTGAAGCAGTTCAAACAGCGATTGATGCA
GGAGCAACTGTCATCAACATTCCAGATACGGTCGGATATACAAACCCCACCGAATTTGGGCAATTATTCCAAGATTTGCG
CCGCGAAATAAAGCAATTTGATGATATTATTTTCGCTTCTCATTGCCATGATGACCTTGGTATGGCGACAGCAAACGCAC
TTGCTGCAATCGAAAATGGCGCAAGACGCGTCGAAGGTACGATTAATGGGATTGGTGAGCGTGCTGGGAATACGGCACTG
GAAGAAGTTGCGGTAGCGCTTCATATTCGCAAAGATTTTTATCAAGCTGAAACAAACATTGTATTGAATCAATTTAAAAA
TTCAAGTGATTTGATTAGCCGCTTGTCTGGTATGCCTGTTCCACGTAACAAAGCTGTAATTGGTGGCAATGCTTACGCGC
ATGAATCTGGTATTCATCAAGATGGTGTCCTAAAAAATCCTGATACGTATGAAATCATCACCCCTGCCCTTGTCGGCGTG
GATAAAAACTCTCTTCCACTTGGCAAACTTTCTGGCAAACATGCCTTCAATACGCGCATGGAAGAAATGGGCTACACACT
AACCGAACAAGAACAAAAAGATGCCTTTAAACGTTTCAAACAACTAGCAGATGCGAAAAAAGAAGTAACAGAAGAAGACT
TGCACGCGTTAATTCTCGGACAATCTTCCGAGTCAGCCGATGATTTCGAACTAAAACATTTGCAAGTTCAATATGTTACT
GGCGGCGTCCAAGGTGCGATTGTTCGCATTGAAGAACGTGATGGCGCTCTAGTAGAAGACGCAGCAACTGGTTCAGGTAG
CATTGAAGCGATTTATAATACGATTAACCGTCTTATGAAACAAGATATTGAATTAACCGATTATCGTATCCAAGCAATTA
CAGCCGGTCAAGATGCCCAAGCAGAAGTCCATGTCGTCATTAAAAATGACAAAGGCGCTGTGTTCCATGGTATCGGTATT
GATTTTGACGTTTTAACTGCTAGTGCTAAAGCTTATTTGCAAGCATCAGGCAAAAGCAAAACCGCCAGTAAGCAAGCTGA
TTTCGAGGAGGTAAAATAA

Upstream 100 bases:

>100_bases
ATATGAGTTTAAGGAAGAACCTCTTTCTTTCCTAAACTGATACTGAAAGCAGGAAAAATCCAAGCTTCCCTATCTAATAT
AAAACAAGAGGTGAAATCGA

Downstream 100 bases:

>100_bases
CGTGACCTATAAAATTACTTCCTTAGCTGGCGACGGAATTGGTCCAGAAATTATGACTTCTGGACTTCAAGTATTAGAGG
CAGTCGAAAAAAAATACAAC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MKKIQFFDTTLRDGEQTPGVNFDVKEKIQIALQLEKLGIDVIEAGFPISSPGDFECVKAIAKAIKHCSVTGLARCVEGDI
DRAEEALKDAVSPQIHIFLATSDVHMEYKLKMSRAEVLASIKHHISYARQKFDVVQFSPEDATRSDRAFLIEAVQTAIDA
GATVINIPDTVGYTNPTEFGQLFQDLRREIKQFDDIIFASHCHDDLGMATANALAAIENGARRVEGTINGIGERAGNTAL
EEVAVALHIRKDFYQAETNIVLNQFKNSSDLISRLSGMPVPRNKAVIGGNAYAHESGIHQDGVLKNPDTYEIITPALVGV
DKNSLPLGKLSGKHAFNTRMEEMGYTLTEQEQKDAFKRFKQLADAKKEVTEEDLHALILGQSSESADDFELKHLQVQYVT
GGVQGAIVRIEERDGALVEDAATGSGSIEAIYNTINRLMKQDIELTDYRIQAITAGQDAQAEVHVVIKNDKGAVFHGIGI
DFDVLTASAKAYLQASGKSKTASKQADFEEVK

Sequences:

>Translated_512_residues
MKKIQFFDTTLRDGEQTPGVNFDVKEKIQIALQLEKLGIDVIEAGFPISSPGDFECVKAIAKAIKHCSVTGLARCVEGDI
DRAEEALKDAVSPQIHIFLATSDVHMEYKLKMSRAEVLASIKHHISYARQKFDVVQFSPEDATRSDRAFLIEAVQTAIDA
GATVINIPDTVGYTNPTEFGQLFQDLRREIKQFDDIIFASHCHDDLGMATANALAAIENGARRVEGTINGIGERAGNTAL
EEVAVALHIRKDFYQAETNIVLNQFKNSSDLISRLSGMPVPRNKAVIGGNAYAHESGIHQDGVLKNPDTYEIITPALVGV
DKNSLPLGKLSGKHAFNTRMEEMGYTLTEQEQKDAFKRFKQLADAKKEVTEEDLHALILGQSSESADDFELKHLQVQYVT
GGVQGAIVRIEERDGALVEDAATGSGSIEAIYNTINRLMKQDIELTDYRIQAITAGQDAQAEVHVVIKNDKGAVFHGIGI
DFDVLTASAKAYLQASGKSKTASKQADFEEVK
>Mature_512_residues
MKKIQFFDTTLRDGEQTPGVNFDVKEKIQIALQLEKLGIDVIEAGFPISSPGDFECVKAIAKAIKHCSVTGLARCVEGDI
DRAEEALKDAVSPQIHIFLATSDVHMEYKLKMSRAEVLASIKHHISYARQKFDVVQFSPEDATRSDRAFLIEAVQTAIDA
GATVINIPDTVGYTNPTEFGQLFQDLRREIKQFDDIIFASHCHDDLGMATANALAAIENGARRVEGTINGIGERAGNTAL
EEVAVALHIRKDFYQAETNIVLNQFKNSSDLISRLSGMPVPRNKAVIGGNAYAHESGIHQDGVLKNPDTYEIITPALVGV
DKNSLPLGKLSGKHAFNTRMEEMGYTLTEQEQKDAFKRFKQLADAKKEVTEEDLHALILGQSSESADDFELKHLQVQYVT
GGVQGAIVRIEERDGALVEDAATGSGSIEAIYNTINRLMKQDIELTDYRIQAITAGQDAQAEVHVVIKNDKGAVFHGIGI
DFDVLTASAKAYLQASGKSKTASKQADFEEVK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=494, Percent_Identity=45.5465587044534, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320071, Length=366, Percent_Identity=30.0546448087432, Blast_Score=163, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6320019, Length=366, Percent_Identity=29.7814207650273, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324682, Length=537, Percent_Identity=25.1396648044693, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324225, Length=531, Percent_Identity=24.2937853107345, Blast_Score=120, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24582381, Length=265, Percent_Identity=25.2830188679245, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56065; Mature: 56065

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIQFFDTTLRDGEQTPGVNFDVKEKIQIALQLEKLGIDVIEAGFPISSPGDFECVKAI
CCCEEEECHHHCCCCCCCCCCCCHHHHHHEEEEHHHHCCEEEECCCCCCCCCCHHHHHHH
AKAIKHCSVTGLARCVEGDIDRAEEALKDAVSPQIHIFLATSDVHMEYKLKMSRAEVLAS
HHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEEECCEEEEEEEHHHHHHHHHH
IKHHISYARQKFDVVQFSPEDATRSDRAFLIEAVQTAIDAGATVINIPDTVGYTNPTEFG
HHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHH
QLFQDLRREIKQFDDIIFASHCHDDLGMATANALAAIENGARRVEGTINGIGERAGNTAL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHH
EEVAVALHIRKDFYQAETNIVLNQFKNSSDLISRLSGMPVPRNKAVIGGNAYAHESGIHQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCC
DGVLKNPDTYEIITPALVGVDKNSLPLGKLSGKHAFNTRMEEMGYTLTEQEQKDAFKRFK
CCCCCCCCCCEEECHHHEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
QLADAKKEVTEEDLHALILGQSSESADDFELKHLQVQYVTGGVQGAIVRIEERDGALVED
HHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEEEEEECCCCEEEEEEECCCCCEEEC
AATGSGSIEAIYNTINRLMKQDIELTDYRIQAITAGQDAQAEVHVVIKNDKGAVFHGIGI
CCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCEEEECCC
DFDVLTASAKAYLQASGKSKTASKQADFEEVK
CHHHHHHHHHHHHEECCCCCCCCCCCCHHHCC
>Mature Secondary Structure
MKKIQFFDTTLRDGEQTPGVNFDVKEKIQIALQLEKLGIDVIEAGFPISSPGDFECVKAI
CCCEEEECHHHCCCCCCCCCCCCHHHHHHEEEEHHHHCCEEEECCCCCCCCCCHHHHHHH
AKAIKHCSVTGLARCVEGDIDRAEEALKDAVSPQIHIFLATSDVHMEYKLKMSRAEVLAS
HHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEEECCEEEEEEEHHHHHHHHHH
IKHHISYARQKFDVVQFSPEDATRSDRAFLIEAVQTAIDAGATVINIPDTVGYTNPTEFG
HHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHH
QLFQDLRREIKQFDDIIFASHCHDDLGMATANALAAIENGARRVEGTINGIGERAGNTAL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHH
EEVAVALHIRKDFYQAETNIVLNQFKNSSDLISRLSGMPVPRNKAVIGGNAYAHESGIHQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCC
DGVLKNPDTYEIITPALVGVDKNSLPLGKLSGKHAFNTRMEEMGYTLTEQEQKDAFKRFK
CCCCCCCCCCEEECHHHEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
QLADAKKEVTEEDLHALILGQSSESADDFELKHLQVQYVTGGVQGAIVRIEERDGALVED
HHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEEEEEECCCCEEEEEEECCCCCEEEC
AATGSGSIEAIYNTINRLMKQDIELTDYRIQAITAGQDAQAEVHVVIKNDKGAVFHGIGI
CCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCEEEECCC
DFDVLTASAKAYLQASGKSKTASKQADFEEVK
CHHHHHHHHHHHHEECCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]