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The map label for this gene is ycdT [H]

Identifier: 226224516

GI number: 226224516

Start: 1973084

End: 1974211

Strand: Direct

Name: ycdT [H]

Synonym: Lm4b_01929

Alternate gene names: 226224516

Gene position: 1973084-1974211 (Clockwise)

Preceding gene: 226224515

Following gene: 226224517

Centisome position: 67.74

GC content: 37.59

Gene sequence:

>1128_bases
TTGTTCGAACTAGTTAATTCATACATAGATAGTTTAGCGCTCTTTCTCGCTATCCTTTTTATCCAAGGGATGTCTTTTCG
AAAAATAAGACAATATCGCCCAAATTGGTTTCAAAATGGTGGTAGACGGCTATTTCTTTCCATCCTACTAGGCGTCTACT
ATGGTTTAGCAGGTATTTATTTTATTTATGAAGGTGCTTACGAAAATAATCCCGTTATTTACACGAATATGCGGATTTTG
ATTTTGATGGTAACAAGTGTTTTCGGTGGCCGCGTTCCACTCATTTTCGCCTATTTAGTAATGCTTTTTGGTCGGATTAG
TTTTGATATTGCTTCTCCTGTTACTAGTCGCTATGTCATTTTGATGACGCTCATTTTTGCCGCGTGTTTACTTGTTACTT
TTTGGAAAAAACAACGCTTTTCGAGATTTGTTGCTTTAATCATTTTAAACTTCCCAGCGATTCTTTATTATTTCGTGAAT
AATTTTGACGAAGGACGAATCCTGCAAGGTTTTGAGATTATCGAGTATTTCCTGCTATTCTTTGTAACCGCCTTACTGGT
ATTTTACGCCTGCAATTACATTGATAAAAGTAATCTCGTTATCCAAAATTTGACGGAAACTGCGATGACGGATAGTTTAA
CTAATTTACCAAACATGCGCTTTTTCACCCAACAGTTTGGTTGGATTTTTGCGAAATCGCAAAAAAGGAAAAAATCTTTA
TCTCTTTTCATCATTGATATTGATCATTTTAAAGAAATAAACGATTTTCATGGTCATCAAGCTGGTAATACCGTTCTTGC
TCAGTTTAGTAGCATTCTTAAAAATCGCACCTTCCCGCCAAGAACCATGTTTGCACGAATTGGTGGCGAAGAATTTGCTG
TTTTACTTCAAAACGTTGGTACAGAGCAAGCTGCTCTAATTGCTGATTTTTTCCGTGAAGAAGTGGAACGAGCGAAGTTT
CCTTATAATCCCGCAAGTGGCAAAGTTACTGTATCTATAGGTGTTGCTTCTGCTAGTTCCAACTTTTCAACAACCGAATC
ACTTTTCGAAGCTGCTGACCAAGCGCTCTATCAAGCAAAACAAAACGGCCGCAATCAAATTGCTGTTCATCAGGGGGAAC
TCGAATGA

Upstream 100 bases:

>100_bases
GATCAAGCGCTATATAATGCGAAACGAAATGGACGAAACCAAGTGCATATTCGCGAAACAGAACCAGTTGTTTAAGAGAG
TAAAAAGGAGATGAGTCCAT

Downstream 100 bases:

>100_bases
AACGATTCATCTTAATTTTCATTTTAATTATCCTTATTGGCGCCGGATTTTTCTTTTTCCTTCGTCCAGAACCTAAAAAA
ACAGTGAGTGCACCAAAAGA

Product: GGDEF domain protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MFELVNSYIDSLALFLAILFIQGMSFRKIRQYRPNWFQNGGRRLFLSILLGVYYGLAGIYFIYEGAYENNPVIYTNMRIL
ILMVTSVFGGRVPLIFAYLVMLFGRISFDIASPVTSRYVILMTLIFAACLLVTFWKKQRFSRFVALIILNFPAILYYFVN
NFDEGRILQGFEIIEYFLLFFVTALLVFYACNYIDKSNLVIQNLTETAMTDSLTNLPNMRFFTQQFGWIFAKSQKRKKSL
SLFIIDIDHFKEINDFHGHQAGNTVLAQFSSILKNRTFPPRTMFARIGGEEFAVLLQNVGTEQAALIADFFREEVERAKF
PYNPASGKVTVSIGVASASSNFSTTESLFEAADQALYQAKQNGRNQIAVHQGELE

Sequences:

>Translated_375_residues
MFELVNSYIDSLALFLAILFIQGMSFRKIRQYRPNWFQNGGRRLFLSILLGVYYGLAGIYFIYEGAYENNPVIYTNMRIL
ILMVTSVFGGRVPLIFAYLVMLFGRISFDIASPVTSRYVILMTLIFAACLLVTFWKKQRFSRFVALIILNFPAILYYFVN
NFDEGRILQGFEIIEYFLLFFVTALLVFYACNYIDKSNLVIQNLTETAMTDSLTNLPNMRFFTQQFGWIFAKSQKRKKSL
SLFIIDIDHFKEINDFHGHQAGNTVLAQFSSILKNRTFPPRTMFARIGGEEFAVLLQNVGTEQAALIADFFREEVERAKF
PYNPASGKVTVSIGVASASSNFSTTESLFEAADQALYQAKQNGRNQIAVHQGELE
>Mature_375_residues
MFELVNSYIDSLALFLAILFIQGMSFRKIRQYRPNWFQNGGRRLFLSILLGVYYGLAGIYFIYEGAYENNPVIYTNMRIL
ILMVTSVFGGRVPLIFAYLVMLFGRISFDIASPVTSRYVILMTLIFAACLLVTFWKKQRFSRFVALIILNFPAILYYFVN
NFDEGRILQGFEIIEYFLLFFVTALLVFYACNYIDKSNLVIQNLTETAMTDSLTNLPNMRFFTQQFGWIFAKSQKRKKSL
SLFIIDIDHFKEINDFHGHQAGNTVLAQFSSILKNRTFPPRTMFARIGGEEFAVLLQNVGTEQAALIADFFREEVERAKF
PYNPASGKVTVSIGVASASSNFSTTESLFEAADQALYQAKQNGRNQIAVHQGELE

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=259, Percent_Identity=30.8880308880309, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787802, Length=142, Percent_Identity=35.2112676056338, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=36.4705882352941, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788381, Length=194, Percent_Identity=29.3814432989691, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786584, Length=159, Percent_Identity=31.4465408805031, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI145693134, Length=166, Percent_Identity=33.7349397590361, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082007, Length=194, Percent_Identity=30.4123711340206, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=29.585798816568, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=29.8780487804878, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787541, Length=193, Percent_Identity=30.5699481865285, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787056, Length=162, Percent_Identity=32.0987654320988, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=185, Percent_Identity=25.4054054054054, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788956, Length=162, Percent_Identity=33.9506172839506, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081977, Length=185, Percent_Identity=29.7297297297297, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42944; Mature: 42944

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCEEEEECCCCC
>Mature Secondary Structure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HCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]