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The map label for this gene is ycdT [H]

Identifier: 226224515

GI number: 226224515

Start: 1971922

End: 1973058

Strand: Direct

Name: ycdT [H]

Synonym: Lm4b_01928

Alternate gene names: 226224515

Gene position: 1971922-1973058 (Clockwise)

Preceding gene: 226224513

Following gene: 226224516

Centisome position: 67.7

GC content: 36.41

Gene sequence:

>1137_bases
ATGCCAAATATTCTTGATTCATTATGGAACAGCATGGCGCTTTTTTTATCAGCGCTTTTTTTTCACGGAATGGCACTGCG
ATCTATTAGAGAAAAAAAGCCTGCTTGGTTCCAAATTAAATTTTCTAATGAAATTATTAATTATGCACTGGGCATTTACT
ACGGCCTTCTAGGTGTTTATTTTATTATCCGCGGATTACCCGGCACAGATTCTGGGATTTATACAGATATGCTACTTAAT
ATTTTAATCGTCCTGCATTTATTTTCTTCGGCAGGTCCTGCTACTATCGCGCTATTACTAATTGTCGCAGGAAAACTTTT
TCTCGGTGATGCGCTGTTTGCCAATTTGATTTACGCGTTTTTAATTATCGGTTTTCACTTTGTATGCATGGAAGTCGCTA
AACTTCGACTTAGTTCCGTTCAAAAAGTCGTTTTAGTCAAACTTAGTGCGATTCCCCTTATGCTATTTTATTTACATCAA
AAAATACATTTATTGTATACCATAAATGACTTACCTGTTTGGATGCTCTATTTTTTGGTTTCTTTTTTTATTACGTTTAT
TATTGTTTCAGCGGCTTATTATATCGATACATCGAATAAGCTAATTTATGATTTGCAGCAATCCACCATCCTTGACCCGC
TAACAGGTTTGACGAATTTCCGTCATTTTGAAAAGGCGTTCGAAGCAGCATTTAACCATGCTACGTTAAAAAAATCGAAT
CTAAGTGTGATTATTATTGATATTGATTATTTTAAACGGGTAAATGATACATATGGCCATTTAGTCGGAAATGGAGTTTT
ATCCACTTTTAGTCAAATGCTTTTAAAAATCAGCTTCCCGCCAAACACAGTGATATCAAGGATTGGCGGCGAGGAATTCG
CCATTATTTTGCCAAATATTAATGTCAGTGAAACGGAGCATTTGGCGGAGAAAATCCGACGTAAAGTCGAAAAAATTGAT
GTTCCGATTGTTGCTTCTAGCTCCGTTATTACTATTTCTGCTGGTATCGCAAATTATGACGGCAGAAATTATCCAAATCC
GAACGAGCTACTAAATGCAGCCGATCAAGCGCTATATAATGCGAAACGAAATGGACGAAACCAAGTGCATATTCGCGAAA
CAGAACCAGTTGTTTAA

Upstream 100 bases:

>100_bases
TTCTTTGTACTGCGTCTCAGCTAAAAATTCATTTATTTTTCCTTCTGCCTATATTAACAGTTCAGCTGATTAGTTATGCT
GAGATATGGGAGATTTTTTT

Downstream 100 bases:

>100_bases
GAGAGTAAAAAGGAGATGAGTCCATTTGTTCGAACTAGTTAATTCATACATAGATAGTTTAGCGCTCTTTCTCGCTATCC
TTTTTATCCAAGGGATGTCT

Product: GGDEF domain protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MPNILDSLWNSMALFLSALFFHGMALRSIREKKPAWFQIKFSNEIINYALGIYYGLLGVYFIIRGLPGTDSGIYTDMLLN
ILIVLHLFSSAGPATIALLLIVAGKLFLGDALFANLIYAFLIIGFHFVCMEVAKLRLSSVQKVVLVKLSAIPLMLFYLHQ
KIHLLYTINDLPVWMLYFLVSFFITFIIVSAAYYIDTSNKLIYDLQQSTILDPLTGLTNFRHFEKAFEAAFNHATLKKSN
LSVIIIDIDYFKRVNDTYGHLVGNGVLSTFSQMLLKISFPPNTVISRIGGEEFAIILPNINVSETEHLAEKIRRKVEKID
VPIVASSSVITISAGIANYDGRNYPNPNELLNAADQALYNAKRNGRNQVHIRETEPVV

Sequences:

>Translated_378_residues
MPNILDSLWNSMALFLSALFFHGMALRSIREKKPAWFQIKFSNEIINYALGIYYGLLGVYFIIRGLPGTDSGIYTDMLLN
ILIVLHLFSSAGPATIALLLIVAGKLFLGDALFANLIYAFLIIGFHFVCMEVAKLRLSSVQKVVLVKLSAIPLMLFYLHQ
KIHLLYTINDLPVWMLYFLVSFFITFIIVSAAYYIDTSNKLIYDLQQSTILDPLTGLTNFRHFEKAFEAAFNHATLKKSN
LSVIIIDIDYFKRVNDTYGHLVGNGVLSTFSQMLLKISFPPNTVISRIGGEEFAIILPNINVSETEHLAEKIRRKVEKID
VPIVASSSVITISAGIANYDGRNYPNPNELLNAADQALYNAKRNGRNQVHIRETEPVV
>Mature_377_residues
PNILDSLWNSMALFLSALFFHGMALRSIREKKPAWFQIKFSNEIINYALGIYYGLLGVYFIIRGLPGTDSGIYTDMLLNI
LIVLHLFSSAGPATIALLLIVAGKLFLGDALFANLIYAFLIIGFHFVCMEVAKLRLSSVQKVVLVKLSAIPLMLFYLHQK
IHLLYTINDLPVWMLYFLVSFFITFIIVSAAYYIDTSNKLIYDLQQSTILDPLTGLTNFRHFEKAFEAAFNHATLKKSNL
SVIIIDIDYFKRVNDTYGHLVGNGVLSTFSQMLLKISFPPNTVISRIGGEEFAIILPNINVSETEHLAEKIRRKVEKIDV
PIVASSSVITISAGIANYDGRNYPNPNELLNAADQALYNAKRNGRNQVHIRETEPVV

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=42.0731707317073, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=37.6543209876543, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87082007, Length=205, Percent_Identity=34.6341463414634, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787802, Length=156, Percent_Identity=37.1794871794872, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=31.9526627218935, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081881, Length=183, Percent_Identity=32.2404371584699, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788381, Length=181, Percent_Identity=29.8342541436464, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787816, Length=170, Percent_Identity=34.7058823529412, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788085, Length=197, Percent_Identity=28.9340101522843, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787541, Length=157, Percent_Identity=32.484076433121, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787056, Length=171, Percent_Identity=29.8245614035088, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=199, Percent_Identity=27.6381909547739, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42521; Mature: 42389

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNILDSLWNSMALFLSALFFHGMALRSIREKKPAWFQIKFSNEIINYALGIYYGLLGVY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHH
FIIRGLPGTDSGIYTDMLLNILIVLHLFSSAGPATIALLLIVAGKLFLGDALFANLIYAF
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIIGFHFVCMEVAKLRLSSVQKVVLVKLSAIPLMLFYLHQKIHLLYTINDLPVWMLYFLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHH
SFFITFIIVSAAYYIDTSNKLIYDLQQSTILDPLTGLTNFRHFEKAFEAAFNHATLKKSN
HHHHHHHHHHHHHEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
LSVIIIDIDYFKRVNDTYGHLVGNGVLSTFSQMLLKISFPPNTVISRIGGEEFAIILPNI
CEEEEEEHHHHHHHCHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCCEEEEECCC
NVSETEHLAEKIRRKVEKIDVPIVASSSVITISAGIANYDGRNYPNPNELLNAADQALYN
CCCHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
AKRNGRNQVHIRETEPVV
HHHCCCCEEEEECCCCCC
>Mature Secondary Structure 
PNILDSLWNSMALFLSALFFHGMALRSIREKKPAWFQIKFSNEIINYALGIYYGLLGVY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHH
FIIRGLPGTDSGIYTDMLLNILIVLHLFSSAGPATIALLLIVAGKLFLGDALFANLIYAF
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIIGFHFVCMEVAKLRLSSVQKVVLVKLSAIPLMLFYLHQKIHLLYTINDLPVWMLYFLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHH
SFFITFIIVSAAYYIDTSNKLIYDLQQSTILDPLTGLTNFRHFEKAFEAAFNHATLKKSN
HHHHHHHHHHHHHEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
LSVIIIDIDYFKRVNDTYGHLVGNGVLSTFSQMLLKISFPPNTVISRIGGEEFAIILPNI
CEEEEEEHHHHHHHCHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCCEEEEECCC
NVSETEHLAEKIRRKVEKIDVPIVASSSVITISAGIANYDGRNYPNPNELLNAADQALYN
CCCHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
AKRNGRNQVHIRETEPVV
HHHCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]