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The map label for this gene is yitJ [H]

Identifier: 226224281

GI number: 226224281

Start: 1719326

End: 1721179

Strand: Reverse

Name: yitJ [H]

Synonym: Lm4b_01691

Alternate gene names: 226224281

Gene position: 1721179-1719326 (Counterclockwise)

Preceding gene: 226224282

Following gene: 226224279

Centisome position: 59.09

GC content: 41.42

Gene sequence:

>1854_bases
ATGAATTTAAGAAAAGATTTAAGTGAAAAAGTATTAATTGCTGACGGCGCGATGGGCACTTTGCTTTATTCTTATGGAGT
GGATCGTTCGTTTGAAGAGCTAAATTTGTCTCATCCGGAAGATATTGTTGCGATTCATAAAGCATACATTGGTGCTGGGG
CGGATATTATTCAAACGAATACGTATGGTGCAAATTATATTAAATTAGCGCGATATGGTTTAGAGGATGAAGTGAAGCGT
ATTAATCAAGCAGCCATTCGGTTAGCAAAGGAAGCGGCAAGAGGGACTGGAACGTATATTTTTGGGACGATTGGTGGAAT
AAATGGCGCAGTGGATGCAAGACTTCCAGCGGCTCCACTCGAAGAAATCAAGCGAAGTTTTAGGGAGCAGCTATACTGTT
TTTTGTTAGATGGTGTGGATGCGATTTTGCTGGAAACGTATTATGATTTAGATGAGCTTAAAACAGTGTTGAAAATTCTT
CGTGAGACTACTGATTTACCGGTAGTTGCGAATGTCTCTATGCATGAGCCGGGTATTCTTCAAAACGGGAAAAAATTGCC
GGATGCGCTGGAAGAACTTATTGCGCTTGGAGCGGATGTAGTTGGTATTAATTGCCGACTTGGTCCTTACCATATGGCGC
GTGCTCTTGAAACAGTGCCGCTGTATGATAATGCTTATTTGGCAGTTTATCCCAACGCTAGTTTGCCAGAGATGCAAGAA
GGAAAAGTAATTTATCAGTCTGACACGGATTATTTTGAACATTATGGGGAGGTTTTTCGGCAAGAAGGTGCTCGGATTAT
TGGTGGTTGTTGTGGCACGACGCCAGATCATATTCGTGCTTTACGAAAAGGCCTTGAAACAACAAAACCCATTTTGGAAA
AAGAAGTTCGGCCGATATTGGAACTTGTTCCAGAAGAAGTGGAGGATGTGGATTCTGGTGAACGTTTGCTTGATAAAGTG
AAGGAACGTTTAACGATTTTGGTTGAACTAGATCCGCCGAGGACTTTTGATACGACGAAATTTTTTGAAGGAGCCCAGGC
GCTTGACGAGGCAGGCGTTGATGCGATTACGATTTCGGATAATTCGCTTGCCACGCCGCGGATTAGTAATATGGCGCTAG
CTTCGATTTTGAAGCATGAATATGGTATTAAACCACTCATTCATTTGACGACGCGGGATCATAATTTGGTTGGGATGCAT
TCGCATGTGATGGGATTTCATAAATTGGGGCTGCATGATGTGCTTGCGATTACAGGTGATCCAACTAAAGTAGGTGATTT
CCCTGGAGCGTCTTCGGTGTTTGATTTAAGGTCTGTGGAATTAGTTCAGCTAATTAAAAAGTTCAATGATGGGATTTCTT
ATACTGGGAAATCACTGAAAGAAAAGGCACGATTCCATGTTGGAGCGGCATTTAATCCGAACGTGCTGAACTTAGAGAAA
GCGGTTAGGTTAATTGAGCGAAAAGTGGAATATGGTGCCGATTATATTATTACACAGCCAATTTATGATGTGAATAAAGC
TATCTTATTAAAAGAAGCCTTGCAAAAAGCAAATATTGATGTGCCACTTTTTATTGGGGTTATGCCATTATTGTCTAGTC
GGAATGCAGAATTTCTGCATAATGAGGTTCCCGGGATTCGTTTGACAGATGATGTGCGGGAGCGAATGAGAGAGGCGGAA
GAGCACGGTCATGCAAATGAAGAAGGCATGGCAATTGCTCGTGAATTAGTGGATGCGATTTGTGAGCATTTCCAAGGGAT
TTATATTATTACGCCATTTTTACGATATGATTTATCGATAGAATTAGCGAAATATGCCCAAAATAAACAGCAAGTCCAAA
TTGGGTCTAAATAA

Upstream 100 bases:

>100_bases
CAGGATTATTACGTTTTTCAGCTGGACTAGAAGATGCCGATGACTTGATTGCTGATTTAAAACAAGCACTTTCTTTCATT
AAGAAGGGAAGTGTTGCGAA

Downstream 100 bases:

>100_bases
AAAAACTGGTTGTCACGAGGACAACCAGTTTTTTTACATGAATAAATTTCTGGATAATAGACTTAAAAGGTAAGCAAGGG
CCAATGTAATACCAAAGATA

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 617; Mature: 617

Protein sequence:

>617_residues
MNLRKDLSEKVLIADGAMGTLLYSYGVDRSFEELNLSHPEDIVAIHKAYIGAGADIIQTNTYGANYIKLARYGLEDEVKR
INQAAIRLAKEAARGTGTYIFGTIGGINGAVDARLPAAPLEEIKRSFREQLYCFLLDGVDAILLETYYDLDELKTVLKIL
RETTDLPVVANVSMHEPGILQNGKKLPDALEELIALGADVVGINCRLGPYHMARALETVPLYDNAYLAVYPNASLPEMQE
GKVIYQSDTDYFEHYGEVFRQEGARIIGGCCGTTPDHIRALRKGLETTKPILEKEVRPILELVPEEVEDVDSGERLLDKV
KERLTILVELDPPRTFDTTKFFEGAQALDEAGVDAITISDNSLATPRISNMALASILKHEYGIKPLIHLTTRDHNLVGMH
SHVMGFHKLGLHDVLAITGDPTKVGDFPGASSVFDLRSVELVQLIKKFNDGISYTGKSLKEKARFHVGAAFNPNVLNLEK
AVRLIERKVEYGADYIITQPIYDVNKAILLKEALQKANIDVPLFIGVMPLLSSRNAEFLHNEVPGIRLTDDVRERMREAE
EHGHANEEGMAIARELVDAICEHFQGIYIITPFLRYDLSIELAKYAQNKQQVQIGSK

Sequences:

>Translated_617_residues
MNLRKDLSEKVLIADGAMGTLLYSYGVDRSFEELNLSHPEDIVAIHKAYIGAGADIIQTNTYGANYIKLARYGLEDEVKR
INQAAIRLAKEAARGTGTYIFGTIGGINGAVDARLPAAPLEEIKRSFREQLYCFLLDGVDAILLETYYDLDELKTVLKIL
RETTDLPVVANVSMHEPGILQNGKKLPDALEELIALGADVVGINCRLGPYHMARALETVPLYDNAYLAVYPNASLPEMQE
GKVIYQSDTDYFEHYGEVFRQEGARIIGGCCGTTPDHIRALRKGLETTKPILEKEVRPILELVPEEVEDVDSGERLLDKV
KERLTILVELDPPRTFDTTKFFEGAQALDEAGVDAITISDNSLATPRISNMALASILKHEYGIKPLIHLTTRDHNLVGMH
SHVMGFHKLGLHDVLAITGDPTKVGDFPGASSVFDLRSVELVQLIKKFNDGISYTGKSLKEKARFHVGAAFNPNVLNLEK
AVRLIERKVEYGADYIITQPIYDVNKAILLKEALQKANIDVPLFIGVMPLLSSRNAEFLHNEVPGIRLTDDVRERMREAE
EHGHANEEGMAIARELVDAICEHFQGIYIITPFLRYDLSIELAKYAQNKQQVQIGSK
>Mature_617_residues
MNLRKDLSEKVLIADGAMGTLLYSYGVDRSFEELNLSHPEDIVAIHKAYIGAGADIIQTNTYGANYIKLARYGLEDEVKR
INQAAIRLAKEAARGTGTYIFGTIGGINGAVDARLPAAPLEEIKRSFREQLYCFLLDGVDAILLETYYDLDELKTVLKIL
RETTDLPVVANVSMHEPGILQNGKKLPDALEELIALGADVVGINCRLGPYHMARALETVPLYDNAYLAVYPNASLPEMQE
GKVIYQSDTDYFEHYGEVFRQEGARIIGGCCGTTPDHIRALRKGLETTKPILEKEVRPILELVPEEVEDVDSGERLLDKV
KERLTILVELDPPRTFDTTKFFEGAQALDEAGVDAITISDNSLATPRISNMALASILKHEYGIKPLIHLTTRDHNLVGMH
SHVMGFHKLGLHDVLAITGDPTKVGDFPGASSVFDLRSVELVQLIKKFNDGISYTGKSLKEKARFHVGAAFNPNVLNLEK
AVRLIERKVEYGADYIITQPIYDVNKAILLKEALQKANIDVPLFIGVMPLLSSRNAEFLHNEVPGIRLTDDVRERMREAE
EHGHANEEGMAIARELVDAICEHFQGIYIITPFLRYDLSIELAKYAQNKQQVQIGSK

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=327, Percent_Identity=27.82874617737, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI13162290, Length=317, Percent_Identity=28.391167192429, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI157266337, Length=313, Percent_Identity=26.1980830670927, Blast_Score=80, Evalue=6e-15,
Organism=Escherichia coli, GI1790450, Length=326, Percent_Identity=30.3680981595092, Blast_Score=117, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17535405, Length=320, Percent_Identity=28.125, Blast_Score=121, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324982, Length=315, Percent_Identity=26.6666666666667, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6322966, Length=310, Percent_Identity=24.8387096774194, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 68621; Mature: 68621

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRKDLSEKVLIADGAMGTLLYSYGVDRSFEELNLSHPEDIVAIHKAYIGAGADIIQTN
CCCHHHCCCCEEEECCCHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHCCCCCEEEEC
TYGANYIKLARYGLEDEVKRINQAAIRLAKEAARGTGTYIFGTIGGINGAVDARLPAAPL
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHH
EEIKRSFREQLYCFLLDGVDAILLETYYDLDELKTVLKILRETTDLPVVANVSMHEPGIL
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
QNGKKLPDALEELIALGADVVGINCRLGPYHMARALETVPLYDNAYLAVYPNASLPEMQE
CCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
GKVIYQSDTDYFEHYGEVFRQEGARIIGGCCGTTPDHIRALRKGLETTKPILEKEVRPIL
CCEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELVPEEVEDVDSGERLLDKVKERLTILVELDPPRTFDTTKFFEGAQALDEAGVDAITISD
HHCCHHHHCCCCHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECC
NSLATPRISNMALASILKHEYGIKPLIHLTTRDHNLVGMHSHVMGFHKLGLHDVLAITGD
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEHHHHHHHHHHCCHHHEEEECCC
PTKVGDFPGASSVFDLRSVELVQLIKKFNDGISYTGKSLKEKARFHVGAAFNPNVLNLEK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEECCCCCCCEECHHH
AVRLIERKVEYGADYIITQPIYDVNKAILLKEALQKANIDVPLFIGVMPLLSSRNAEFLH
HHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHH
NEVPGIRLTDDVRERMREAEEHGHANEEGMAIARELVDAICEHFQGIYIITPFLRYDLSI
CCCCCCEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHCCHH
ELAKYAQNKQQVQIGSK
HHHHHCCCCCCEECCCC
>Mature Secondary Structure
MNLRKDLSEKVLIADGAMGTLLYSYGVDRSFEELNLSHPEDIVAIHKAYIGAGADIIQTN
CCCHHHCCCCEEEECCCHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHCCCCCEEEEC
TYGANYIKLARYGLEDEVKRINQAAIRLAKEAARGTGTYIFGTIGGINGAVDARLPAAPL
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHH
EEIKRSFREQLYCFLLDGVDAILLETYYDLDELKTVLKILRETTDLPVVANVSMHEPGIL
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
QNGKKLPDALEELIALGADVVGINCRLGPYHMARALETVPLYDNAYLAVYPNASLPEMQE
CCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
GKVIYQSDTDYFEHYGEVFRQEGARIIGGCCGTTPDHIRALRKGLETTKPILEKEVRPIL
CCEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELVPEEVEDVDSGERLLDKVKERLTILVELDPPRTFDTTKFFEGAQALDEAGVDAITISD
HHCCHHHHCCCCHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECC
NSLATPRISNMALASILKHEYGIKPLIHLTTRDHNLVGMHSHVMGFHKLGLHDVLAITGD
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEHHHHHHHHHHCCHHHEEEECCC
PTKVGDFPGASSVFDLRSVELVQLIKKFNDGISYTGKSLKEKARFHVGAAFNPNVLNLEK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEECCCCCCCEECHHH
AVRLIERKVEYGADYIITQPIYDVNKAILLKEALQKANIDVPLFIGVMPLLSSRNAEFLH
HHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHH
NEVPGIRLTDDVRERMREAEEHGHANEEGMAIARELVDAICEHFQGIYIITPFLRYDLSI
CCCCCCEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHCCHH
ELAKYAQNKQQVQIGSK
HHHHHCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]