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The map label for this gene is menF

Identifier: 226224279

GI number: 226224279

Start: 1716791

End: 1718179

Strand: Reverse

Name: menF

Synonym: Lm4b_01689

Alternate gene names: 226224279

Gene position: 1718179-1716791 (Counterclockwise)

Preceding gene: 226224281

Following gene: 226224278

Centisome position: 58.99

GC content: 39.31

Gene sequence:

>1389_bases
ATGAATACTAAATTGCCTCTTGATTTATTTAATCAAGCGAAACGTAGCGCAAGCCAAGATCAACCGGCTCTATTTAGCTG
GGTAACTGAGTTAGATGAATTAGTGTCTCCAGTCAAATTGTTTAAAAAAGCTGGCACGGCTTTTAAAGGTGAGCGTTTTT
TCTGGCAAAATCCAGAAATGACACTGACCATGACCGGTTTTGGTGTAACGAAACAATTTTTGGCGGAGAAGAAAAAAGAT
GCTTTTCTTGGACTGCAAGAGAAAATTGAGAAGAGACTGCGAACTAGTGTAACCAATGCAACCGTAGATGCTACTGGACC
AATTTACTTTGGTGGTTTTGCCTTTGATCCTGAGCGTGATACAGAGAAAGAATGGCAAAGTTTTAAAGATGGCTTGTTTT
ATTTACCACTATTTATGTTAACGAATAAAGATGGTAAAAGTTACTTAACCGTTAATTTGTCTATCTACTCTGATGATACG
GAAAGCAAATTAGAAGCTGTTTTTAATCAATGGCAAAAAATCATTCATCAAGAGGTTAATGAAGCTGAAATGGCATTACT
TACAGATTTTAAGGAGATTGGCAAAGACCATTTCCTAGAAACTGCGAGTGAAATTATCGATATGATTAATCATTCAGAAG
ATGTAAAAAAAGTGGTTCTAGCTAGACGAATGGGCTTGCGTTTCCAGCGCCAAGTAGATAGTGCCATTATTTTGGAAAAT
ATGCTAGCAACACAAGAAAATAGTTATTTCTTCCTTATTGAAAAGGGAACAGGGGTCTTTTTCGGGGCAACTCCTGAAAG
ATTACTTGCGGTCAATGGGGATGAAATTCATTCGTCATGTGTAGCTGGTTCTACGGAGCGCGGCGCTACCGAAGAAGCGG
ACGAAGCACTTGGAAATGCGCTGTTAAAAGATGCGAAAAACTTGCGAGAGCATTCTTATGTAGTGCAAATGATGGAAGAA
ACATTAAAACCATTTACAACAGGATTGTCGTTATCTAGCCAACCAGTACTTCTGAAAAATCGTGATATTCAACATCTATA
TATGAATATTACCGCGAAGAAAAAACCAGATGTTTCCATGCTTGAAATGGTAAAAGCTCTTCATCCAACGCCAGCTCTTG
GCGGTTTACCTCAAAAAATGGGATTAGCCATTATTCGGATGAAAGAAGAAATGGATCGTGGTTTCTACGGAGCACCGATT
GGCTGGGTTGACTTAAAAGGTTCTGGTGAGTTTGCTGTGGCCATTCGTTCAGGCTTAATTGTCGATTCGCAGGGGTTAAT
CTATGCTGGTTGCGGGATTGTTGGAGATTCTGTACCAAAAGAAGAATTACAAGAAACGGCAGTAAAATTCCAGCCGATGT
TACGCGTATTAGGAGGCATAAAGAAATGA

Upstream 100 bases:

>100_bases
CAATAATTAATGACGAAATGAAAGAAAAAACGCAAACCAAGCAGTTTAGCTCTATTTTTGGTATAATAGAAGATGTGAAT
GTAAAAGGAGAGATAAGCAT

Downstream 100 bases:

>100_bases
CAAACCACGAACAAGTGCTGACAGATTATTTAGCTGCATTTATTGAGGAGCTTGTGCAAGCCGGTGTGAAAGAAGCAATT
ATTAGCCCGGGTTCACGTTC

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MNTKLPLDLFNQAKRSASQDQPALFSWVTELDELVSPVKLFKKAGTAFKGERFFWQNPEMTLTMTGFGVTKQFLAEKKKD
AFLGLQEKIEKRLRTSVTNATVDATGPIYFGGFAFDPERDTEKEWQSFKDGLFYLPLFMLTNKDGKSYLTVNLSIYSDDT
ESKLEAVFNQWQKIIHQEVNEAEMALLTDFKEIGKDHFLETASEIIDMINHSEDVKKVVLARRMGLRFQRQVDSAIILEN
MLATQENSYFFLIEKGTGVFFGATPERLLAVNGDEIHSSCVAGSTERGATEEADEALGNALLKDAKNLREHSYVVQMMEE
TLKPFTTGLSLSSQPVLLKNRDIQHLYMNITAKKKPDVSMLEMVKALHPTPALGGLPQKMGLAIIRMKEEMDRGFYGAPI
GWVDLKGSGEFAVAIRSGLIVDSQGLIYAGCGIVGDSVPKEELQETAVKFQPMLRVLGGIKK

Sequences:

>Translated_462_residues
MNTKLPLDLFNQAKRSASQDQPALFSWVTELDELVSPVKLFKKAGTAFKGERFFWQNPEMTLTMTGFGVTKQFLAEKKKD
AFLGLQEKIEKRLRTSVTNATVDATGPIYFGGFAFDPERDTEKEWQSFKDGLFYLPLFMLTNKDGKSYLTVNLSIYSDDT
ESKLEAVFNQWQKIIHQEVNEAEMALLTDFKEIGKDHFLETASEIIDMINHSEDVKKVVLARRMGLRFQRQVDSAIILEN
MLATQENSYFFLIEKGTGVFFGATPERLLAVNGDEIHSSCVAGSTERGATEEADEALGNALLKDAKNLREHSYVVQMMEE
TLKPFTTGLSLSSQPVLLKNRDIQHLYMNITAKKKPDVSMLEMVKALHPTPALGGLPQKMGLAIIRMKEEMDRGFYGAPI
GWVDLKGSGEFAVAIRSGLIVDSQGLIYAGCGIVGDSVPKEELQETAVKFQPMLRVLGGIKK
>Mature_462_residues
MNTKLPLDLFNQAKRSASQDQPALFSWVTELDELVSPVKLFKKAGTAFKGERFFWQNPEMTLTMTGFGVTKQFLAEKKKD
AFLGLQEKIEKRLRTSVTNATVDATGPIYFGGFAFDPERDTEKEWQSFKDGLFYLPLFMLTNKDGKSYLTVNLSIYSDDT
ESKLEAVFNQWQKIIHQEVNEAEMALLTDFKEIGKDHFLETASEIIDMINHSEDVKKVVLARRMGLRFQRQVDSAIILEN
MLATQENSYFFLIEKGTGVFFGATPERLLAVNGDEIHSSCVAGSTERGATEEADEALGNALLKDAKNLREHSYVVQMMEE
TLKPFTTGLSLSSQPVLLKNRDIQHLYMNITAKKKPDVSMLEMVKALHPTPALGGLPQKMGLAIIRMKEEMDRGFYGAPI
GWVDLKGSGEFAVAIRSGLIVDSQGLIYAGCGIVGDSVPKEELQETAVKFQPMLRVLGGIKK

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=251, Percent_Identity=35.8565737051793, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI87082077, Length=235, Percent_Identity=32.7659574468085, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787518, Length=392, Percent_Identity=22.9591836734694, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788114, Length=254, Percent_Identity=24.4094488188976, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320935, Length=218, Percent_Identity=25.6880733944954, Blast_Score=77, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 51682; Mature: 51682

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKLPLDLFNQAKRSASQDQPALFSWVTELDELVSPVKLFKKAGTAFKGERFFWQNPEM
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCE
TLTMTGFGVTKQFLAEKKKDAFLGLQEKIEKRLRTSVTNATVDATGPIYFGGFAFDPERD
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEECCEEECCCCC
TEKEWQSFKDGLFYLPLFMLTNKDGKSYLTVNLSIYSDDTESKLEAVFNQWQKIIHQEVN
CHHHHHHHHCCHHHEEHHEEECCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHC
EAEMALLTDFKEIGKDHFLETASEIIDMINHSEDVKKVVLARRMGLRFQRQVDSAIILEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
MLATQENSYFFLIEKGTGVFFGATPERLLAVNGDEIHSSCVAGSTERGATEEADEALGNA
HHHCCCCCEEEEEECCCEEEECCCCHHEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHH
LLKDAKNLREHSYVVQMMEETLKPFTTGLSLSSQPVLLKNRDIQHLYMNITAKKKPDVSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEEECCCCCCHHH
LEMVKALHPTPALGGLPQKMGLAIIRMKEEMDRGFYGAPIGWVDLKGSGEFAVAIRSGLI
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEECCEE
VDSQGLIYAGCGIVGDSVPKEELQETAVKFQPMLRVLGGIKK
ECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNTKLPLDLFNQAKRSASQDQPALFSWVTELDELVSPVKLFKKAGTAFKGERFFWQNPEM
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCE
TLTMTGFGVTKQFLAEKKKDAFLGLQEKIEKRLRTSVTNATVDATGPIYFGGFAFDPERD
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEECCEEECCCCC
TEKEWQSFKDGLFYLPLFMLTNKDGKSYLTVNLSIYSDDTESKLEAVFNQWQKIIHQEVN
CHHHHHHHHCCHHHEEHHEEECCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHC
EAEMALLTDFKEIGKDHFLETASEIIDMINHSEDVKKVVLARRMGLRFQRQVDSAIILEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
MLATQENSYFFLIEKGTGVFFGATPERLLAVNGDEIHSSCVAGSTERGATEEADEALGNA
HHHCCCCCEEEEEECCCEEEECCCCHHEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHH
LLKDAKNLREHSYVVQMMEETLKPFTTGLSLSSQPVLLKNRDIQHLYMNITAKKKPDVSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEEECCCCCCHHH
LEMVKALHPTPALGGLPQKMGLAIIRMKEEMDRGFYGAPIGWVDLKGSGEFAVAIRSGLI
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEECCEE
VDSQGLIYAGCGIVGDSVPKEELQETAVKFQPMLRVLGGIKK
ECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]