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The map label for this gene is menD

Identifier: 226224278

GI number: 226224278

Start: 1715052

End: 1716794

Strand: Reverse

Name: menD

Synonym: Lm4b_01688

Alternate gene names: 226224278

Gene position: 1716794-1715052 (Counterclockwise)

Preceding gene: 226224279

Following gene: 226224277

Centisome position: 58.94

GC content: 40.73

Gene sequence:

>1743_bases
ATGACAAACCACGAACAAGTGCTGACAGATTATTTAGCTGCATTTATTGAGGAGCTTGTGCAAGCCGGTGTGAAAGAAGC
AATTATTAGCCCGGGTTCACGTTCCACTCCGCTTGCACTGATGATGGCAGAGCATCCGATTTTGAAGATTTATGTGGATG
TTGACGAACGCTCGGCTGGCTTTTTTGCTTTAGGTCTAGCGAAAGCATCGAAACGTCCAGTTGTTCTACTTTGTACTTCA
GGAACGGCAGCTGCAAACTATTTTCCAGCAGTTGCTGAAGCTAATTTATCACAAATCCCATTGATTGTTTTAACTGCTGA
TCGTCCACATGAGCTTCGAAATGTTGGAGCACCACAAGCGATGGATCAGTTGCATTTATATGGTTCACATGTCAAGGATT
TTACGGATATGGCACTTCCTGAAAACAGTGAAGAAATGCTTCGCTACGCAAAATGGCATGGAAGTCGCGCGGTGGATATT
GCAATGAAAACTCCACGTGGCCCTGTGCACCTCAATTTCCCATTACGCGAACCGCTTGTGCCAATTCTAGAACCTTCACC
GTTCACAGCTACCGGAAAAAAACATCATCACGTACATATTTATTATACGCATGAAGTATTGGACGATAGCTCGATTCAAA
AAATGGTGACAGAATGCACTGGTAAAAAAGGCGTATTCGTCGTTGGTCCAATCGATAAGAAAGAATTGGAGCAGCCAATG
GTTGATTTAGCGAAAAAACTAGGTTGGCCGATTTTAGCTGATCCGCTTTCTGGACTCCGTTCTTACGGGGCGCTAGATGA
AGTAGTGATTGACCAATATGATGCATTCTTAAAAGAAGCAGAGATTATGGATAAATTAACACCAGAAGTAGTTATTCGTT
TTGGAAGTATGCCAGTTTCTAAACCACTGAAAAACTGGTTAGAACAACTTTCAGATATTCGTTTTTATGTGGTGGATCCG
GGTGCTGCTTGGAAAGATCCGATTAAAGCGGTAACAGACATGATTCACTGTGATGAACGATTTTTACTAGATATCATGCA
ACAAAATATGCCTGATGATGCGAAAGATGCAGCGTGGTTAAATGGATGGACATCGTATAATAAAGTGGCACGAGAAATCG
TTTTGGCTGAAATGGCGAACACAACCATTTTGGAAGAAGGTAAGATAGTTGCGGAATTACGCCGTTTGTTACCGGACAAA
GCGGGACTCTTTATTGGTAATAGTATGCCGATTCGCGATGTTGACACGTACTTTTCACAAATTGATAAGAAAATAAAAAT
GCTAGCAAATCGCGGGGCTAATGGTATTGACGGAGTTGTTTCTTCGGCGTTAGGTGCTAGTGTTGTCTTCCAACCAATGT
TTTTACTAATTGGTGATTTATCCTTTTATCATGATATGAACGGGTTACTTATGGCGAAAAAATATAAGATGAACCTAACG
ATTGTGATTGTTAATAATGATGGCGGCGGGATATTCTCGTTCTTACCTCAAGCAAACGAGCCGAAGTATTTTGAATCATT
ATTTGGTACATCTACTGAACTTGATTTCCGTTTTGCAGCTGCTTTTTATGATGCAGATTATCACGAGGCTAAGTCGGTAG
ATGAACTAGAAGAAGCAATCGATAAAGCTAGCTACCATAAAGGTTTGGATATTATTGAAGTGAAAACAAATCGTCATGAA
AATAAAGCAAATCATCAAGCACTTTGGGTTAAAATTGCGGACGCGTTAAAGGCATTAGATTAA

Upstream 100 bases:

>100_bases
CTGGTTGCGGGATTGTTGGAGATTCTGTACCAAAAGAAGAATTACAAGAAACGGCAGTAAAATTCCAGCCGATGTTACGC
GTATTAGGAGGCATAAAGAA

Downstream 100 bases:

>100_bases
TATGCTAGTAAACGGTCGACATTATCATCTGACAACTGCTATAAGTGGAGAAAAGCCAGCTTTACTGATGTTACATGGTT
TTACTGGAACAAGCGAAACA

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 580; Mature: 579

Protein sequence:

>580_residues
MTNHEQVLTDYLAAFIEELVQAGVKEAIISPGSRSTPLALMMAEHPILKIYVDVDERSAGFFALGLAKASKRPVVLLCTS
GTAAANYFPAVAEANLSQIPLIVLTADRPHELRNVGAPQAMDQLHLYGSHVKDFTDMALPENSEEMLRYAKWHGSRAVDI
AMKTPRGPVHLNFPLREPLVPILEPSPFTATGKKHHHVHIYYTHEVLDDSSIQKMVTECTGKKGVFVVGPIDKKELEQPM
VDLAKKLGWPILADPLSGLRSYGALDEVVIDQYDAFLKEAEIMDKLTPEVVIRFGSMPVSKPLKNWLEQLSDIRFYVVDP
GAAWKDPIKAVTDMIHCDERFLLDIMQQNMPDDAKDAAWLNGWTSYNKVAREIVLAEMANTTILEEGKIVAELRRLLPDK
AGLFIGNSMPIRDVDTYFSQIDKKIKMLANRGANGIDGVVSSALGASVVFQPMFLLIGDLSFYHDMNGLLMAKKYKMNLT
IVIVNNDGGGIFSFLPQANEPKYFESLFGTSTELDFRFAAAFYDADYHEAKSVDELEEAIDKASYHKGLDIIEVKTNRHE
NKANHQALWVKIADALKALD

Sequences:

>Translated_580_residues
MTNHEQVLTDYLAAFIEELVQAGVKEAIISPGSRSTPLALMMAEHPILKIYVDVDERSAGFFALGLAKASKRPVVLLCTS
GTAAANYFPAVAEANLSQIPLIVLTADRPHELRNVGAPQAMDQLHLYGSHVKDFTDMALPENSEEMLRYAKWHGSRAVDI
AMKTPRGPVHLNFPLREPLVPILEPSPFTATGKKHHHVHIYYTHEVLDDSSIQKMVTECTGKKGVFVVGPIDKKELEQPM
VDLAKKLGWPILADPLSGLRSYGALDEVVIDQYDAFLKEAEIMDKLTPEVVIRFGSMPVSKPLKNWLEQLSDIRFYVVDP
GAAWKDPIKAVTDMIHCDERFLLDIMQQNMPDDAKDAAWLNGWTSYNKVAREIVLAEMANTTILEEGKIVAELRRLLPDK
AGLFIGNSMPIRDVDTYFSQIDKKIKMLANRGANGIDGVVSSALGASVVFQPMFLLIGDLSFYHDMNGLLMAKKYKMNLT
IVIVNNDGGGIFSFLPQANEPKYFESLFGTSTELDFRFAAAFYDADYHEAKSVDELEEAIDKASYHKGLDIIEVKTNRHE
NKANHQALWVKIADALKALD
>Mature_579_residues
TNHEQVLTDYLAAFIEELVQAGVKEAIISPGSRSTPLALMMAEHPILKIYVDVDERSAGFFALGLAKASKRPVVLLCTSG
TAAANYFPAVAEANLSQIPLIVLTADRPHELRNVGAPQAMDQLHLYGSHVKDFTDMALPENSEEMLRYAKWHGSRAVDIA
MKTPRGPVHLNFPLREPLVPILEPSPFTATGKKHHHVHIYYTHEVLDDSSIQKMVTECTGKKGVFVVGPIDKKELEQPMV
DLAKKLGWPILADPLSGLRSYGALDEVVIDQYDAFLKEAEIMDKLTPEVVIRFGSMPVSKPLKNWLEQLSDIRFYVVDPG
AAWKDPIKAVTDMIHCDERFLLDIMQQNMPDDAKDAAWLNGWTSYNKVAREIVLAEMANTTILEEGKIVAELRRLLPDKA
GLFIGNSMPIRDVDTYFSQIDKKIKMLANRGANGIDGVVSSALGASVVFQPMFLLIGDLSFYHDMNGLLMAKKYKMNLTI
VIVNNDGGGIFSFLPQANEPKYFESLFGTSTELDFRFAAAFYDADYHEAKSVDELEEAIDKASYHKGLDIIEVKTNRHEN
KANHQALWVKIADALKALD

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=570, Percent_Identity=30.5263157894737, Blast_Score=234, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 64599; Mature: 64468

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNHEQVLTDYLAAFIEELVQAGVKEAIISPGSRSTPLALMMAEHPILKIYVDVDERSAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEEECCCCCC
FFALGLAKASKRPVVLLCTSGTAAANYFPAVAEANLSQIPLIVLTADRPHELRNVGAPQA
EEEEEEHHCCCCCEEEEECCCCCCHHCCCHHHCCCCCCCCEEEEECCCCHHHHHCCCCHH
MDQLHLYGSHVKDFTDMALPENSEEMLRYAKWHGSRAVDIAMKTPRGPVHLNFPLREPLV
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEECCCCCCCCC
PILEPSPFTATGKKHHHVHIYYTHEVLDDSSIQKMVTECTGKKGVFVVGPIDKKELEQPM
CCCCCCCCCCCCCCCEEEEEEEEHHHHCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHH
VDLAKKLGWPILADPLSGLRSYGALDEVVIDQYDAFLKEAEIMDKLTPEVVIRFGSMPVS
HHHHHHCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCH
KPLKNWLEQLSDIRFYVVDPGAAWKDPIKAVTDMIHCDERFLLDIMQQNMPDDAKDAAWL
HHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHH
NGWTSYNKVAREIVLAEMANTTILEEGKIVAELRRLLPDKAGLFIGNSMPIRDVDTYFSQ
CCCHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHH
IDKKIKMLANRGANGIDGVVSSALGASVVFQPMFLLIGDLSFYHDMNGLLMAKKYKMNLT
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEE
IVIVNNDGGGIFSFLPQANEPKYFESLFGTSTELDFRFAAAFYDADYHEAKSVDELEEAI
EEEEECCCCCCEEECCCCCCCHHHHHHCCCCCCCCEEEEEEECCCCHHHHCCHHHHHHHH
DKASYHKGLDIIEVKTNRHENKANHQALWVKIADALKALD
HHHHHHCCCEEEEEECCCCCCCCCCHHHHEEHHHHHHHCC
>Mature Secondary Structure 
TNHEQVLTDYLAAFIEELVQAGVKEAIISPGSRSTPLALMMAEHPILKIYVDVDERSAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEEECCCCCC
FFALGLAKASKRPVVLLCTSGTAAANYFPAVAEANLSQIPLIVLTADRPHELRNVGAPQA
EEEEEEHHCCCCCEEEEECCCCCCHHCCCHHHCCCCCCCCEEEEECCCCHHHHHCCCCHH
MDQLHLYGSHVKDFTDMALPENSEEMLRYAKWHGSRAVDIAMKTPRGPVHLNFPLREPLV
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEECCCCCCCCC
PILEPSPFTATGKKHHHVHIYYTHEVLDDSSIQKMVTECTGKKGVFVVGPIDKKELEQPM
CCCCCCCCCCCCCCCEEEEEEEEHHHHCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHH
VDLAKKLGWPILADPLSGLRSYGALDEVVIDQYDAFLKEAEIMDKLTPEVVIRFGSMPVS
HHHHHHCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCH
KPLKNWLEQLSDIRFYVVDPGAAWKDPIKAVTDMIHCDERFLLDIMQQNMPDDAKDAAWL
HHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHH
NGWTSYNKVAREIVLAEMANTTILEEGKIVAELRRLLPDKAGLFIGNSMPIRDVDTYFSQ
CCCHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHH
IDKKIKMLANRGANGIDGVVSSALGASVVFQPMFLLIGDLSFYHDMNGLLMAKKYKMNLT
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEE
IVIVNNDGGGIFSFLPQANEPKYFESLFGTSTELDFRFAAAFYDADYHEAKSVDELEEAI
EEEEECCCCCCEEECCCCCCCHHHHHHCCCCCCCCEEEEEEECCCCHHHHCCHHHHHHHH
DKASYHKGLDIIEVKTNRHENKANHQALWVKIADALKALD
HHHHHHCCCEEEEEECCCCCCCCCCHHHHEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]