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The map label for this gene is leuS

Identifier: 226224263

GI number: 226224263

Start: 1695242

End: 1697653

Strand: Reverse

Name: leuS

Synonym: Lm4b_01673

Alternate gene names: 226224263

Gene position: 1697653-1695242 (Counterclockwise)

Preceding gene: 226224266

Following gene: 226224262

Centisome position: 58.28

GC content: 40.88

Gene sequence:

>2412_bases
GTGACATTTAATCACAAAAAAATGGAACCAAAATGGCAACAATATTGGAGTGAACATAACACTTTCAAAACAACAGAAGA
TAAAAATAAAGATAACTTCTATGCACTAGATATGTTCCCTTACCCATCTGGAGCAGGTCTTCACGTAGGACATCCAGAAG
GTTATACAGCAACAGATATTTTGTCTCGTATGAAACGAATGCAAGGGAAGAACGTCCTTCATCCAATCGGTTGGGATGCT
TTTGGTCTTCCTGCAGAGCAATATGCAATTGATACTGGTAACGACCCAGAAGAATTTACCGCACTGAATATTGCCAACTT
TACTCGCCAAATTAAATCACTTGGTTTTTCCTACGACTGGGACCGTGAAATCAATACAACAGATCCTGAATATTATAAAT
GGACACAGTGGATTTTTGAAAAACTATATGAAAAAGGTTTAGCTTATGAAGCCGAAATCGCTGTAAACTGGTGTCCGGCA
CTTGGTACTGTTTTAGCCAATGAAGAAGTTATCGATGGCAAAAGTGAACGCGGTGGCTTCCCAGTTTTCCGCAAACCAAT
GCGTCAATGGATGCTTAAAATTACCGCGTACGCAGATCGTTTGCTAGATGATCTTGATCTAGTGGATTGGCCTGAAAATA
TTAAAGATATGCAACGTAACTGGATTGGTCGTTCAGAAGGCGCAGAAGTTACTTTTAAAATTAAAGATAGCGATGAAACG
TTTAATGTCTTTACCACACGTCCAGATACACTTTTTGGCGCAACATATACCGTTTTTGCACCAGAACACGAACTTATCGA
AAAAATCACAACACCAGAACAAAAAGAAGCAGTTGAAGCTTATAAAAAACAAGTAGAACTTAAAAGCGAACTGGAAAGAA
CCGACCTTGCAAAAGATAAAACAGGTGTCTTCACAGGAGCTTATGCGATTAATCCAATCAACGGCGAAGAAGTGCCAATT
TGGATTGCGGACTACGTGTTGATTCAATATGGAACAGGTGCGATTATGGCCGTACCAGCTCACGATGAACGTGACTTTGA
ATTTGCGCAACAATTTGGTTTGAATATTCGTCCTGTTCTTGAAGGCGGCGACGTGACAAAAGAAGCATTCACTGGCGACG
GACCACATATTAACTCCGATTTCTTGAACGGACTAGCTAAAGCCGAAGCGATTACCGCTGCAATTGATTGGCTAGAAAAA
GAAGGCATCGGTAGCCGTAAAATCACGTATCGTTTGCGCGACTGGTTGTTCAGCAGACAACGTTACTGGGGTGAACCAAT
TCCAGTTATTCACTGGGAAGACGGGGAAACAACGCTTGTACCAGAAGACGAACTACCACTACTTCTGCCAAAAGCAACAG
AAATCAAACCATCAGGAACAGGCGAATCACCACTTGCTAACTTGCATGACTGGGTAAACGTAACAGACAAAAACGGTCGA
AAAGGTCGTCGCGAAACAAATACAATGCCACAATGGGCTGGCTCAAGCTGGTATTTCTTGCGTTATATCGATCCAAATAA
CAGCGAAGCAATTGCTGACAAAGAAAAACTAGCAGAATGGCTTCCAGTTGACGTTTATATCGGCGGGGCAGAGCATGCAG
TACTTCACCTTCTTTATGCACGTTTCTGGCATAAATTCTTGTACGATATCGGTGTAGTTCCAACAAAAGAACCTTTCCAA
AAACTATTTAACCAAGGTATGATTCTTGGCGAAAATAACGAAAAAATGTCCAAATCAAGAGGTAATGTTGTTAACCCTGA
TGAAGTAGTAGAAAAATACGGCGCAGACACACTTCGCTTGTACGAAATGTTCATGGGACCACTAGAAGCTTCGATTGCAT
GGAACGAAAATGGACTGGAAGGTGCACGTAAATTCCTAGACCGCATCTGGCGCTTGCTAGTAACTGAAGAAGGCACACTC
GCTGAAAAAGTGACAACTGATGCGAATGCTAACTTAGAAAAAGCATATCATCATATGGTGAAAACAGTTACCAACCATTA
CGAAAACCTGCGTTTCAATACCGGGATTTCGCAACTGATGATTTTCATCAATGAAGCATATAAACAAGATACGATTCCAA
AACAATATGTCGAAGGTTTCGTTCAACTCTTGTCACCAATCGCACCACACCTTGCAGAAGAATTATGGGAAATCCTAGGT
CACACCGAAACAATCAGCTACGTAGCTTGGCCAACATACGACGAAACAAAACTAGTAGAAGATGAAGTAGAAATCGTACT
TCAAGTAAACGGCAAAGTAAAAAGCAAAATCACCGTTGCCAAATCTCTCGAAAAAGAAGAACTAGAAAAAATTGCACAAG
AAGACAACAAAATGAAAGAAAACCTAGAAGGCAAAACAATCCGTAAAGTGATTGTCGTTCCAGGGAAACTTGTAAATATT
GTTGCGAACTAA

Upstream 100 bases:

>100_bases
GCGGGAGAAGCATCTCTCGTCCCATGGCGATAAGCCGTTGGGATGTAGGGATTTTTTTGTAGCCAAAATTCGATATTTTA
AGGAAAAGAGGATGAGTAAA

Downstream 100 bases:

>100_bases
TAATGGAAACTGCTGGCTTCTGGCTGGCAGTTTTATTTTTGACTAATTTTTTGGTATAATTACGGAAAATGGAATGGAGA
AAAGTTAATGAGTAATGGTA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 803; Mature: 802

Protein sequence:

>803_residues
MTFNHKKMEPKWQQYWSEHNTFKTTEDKNKDNFYALDMFPYPSGAGLHVGHPEGYTATDILSRMKRMQGKNVLHPIGWDA
FGLPAEQYAIDTGNDPEEFTALNIANFTRQIKSLGFSYDWDREINTTDPEYYKWTQWIFEKLYEKGLAYEAEIAVNWCPA
LGTVLANEEVIDGKSERGGFPVFRKPMRQWMLKITAYADRLLDDLDLVDWPENIKDMQRNWIGRSEGAEVTFKIKDSDET
FNVFTTRPDTLFGATYTVFAPEHELIEKITTPEQKEAVEAYKKQVELKSELERTDLAKDKTGVFTGAYAINPINGEEVPI
WIADYVLIQYGTGAIMAVPAHDERDFEFAQQFGLNIRPVLEGGDVTKEAFTGDGPHINSDFLNGLAKAEAITAAIDWLEK
EGIGSRKITYRLRDWLFSRQRYWGEPIPVIHWEDGETTLVPEDELPLLLPKATEIKPSGTGESPLANLHDWVNVTDKNGR
KGRRETNTMPQWAGSSWYFLRYIDPNNSEAIADKEKLAEWLPVDVYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQ
KLFNQGMILGENNEKMSKSRGNVVNPDEVVEKYGADTLRLYEMFMGPLEASIAWNENGLEGARKFLDRIWRLLVTEEGTL
AEKVTTDANANLEKAYHHMVKTVTNHYENLRFNTGISQLMIFINEAYKQDTIPKQYVEGFVQLLSPIAPHLAEELWEILG
HTETISYVAWPTYDETKLVEDEVEIVLQVNGKVKSKITVAKSLEKEELEKIAQEDNKMKENLEGKTIRKVIVVPGKLVNI
VAN

Sequences:

>Translated_803_residues
MTFNHKKMEPKWQQYWSEHNTFKTTEDKNKDNFYALDMFPYPSGAGLHVGHPEGYTATDILSRMKRMQGKNVLHPIGWDA
FGLPAEQYAIDTGNDPEEFTALNIANFTRQIKSLGFSYDWDREINTTDPEYYKWTQWIFEKLYEKGLAYEAEIAVNWCPA
LGTVLANEEVIDGKSERGGFPVFRKPMRQWMLKITAYADRLLDDLDLVDWPENIKDMQRNWIGRSEGAEVTFKIKDSDET
FNVFTTRPDTLFGATYTVFAPEHELIEKITTPEQKEAVEAYKKQVELKSELERTDLAKDKTGVFTGAYAINPINGEEVPI
WIADYVLIQYGTGAIMAVPAHDERDFEFAQQFGLNIRPVLEGGDVTKEAFTGDGPHINSDFLNGLAKAEAITAAIDWLEK
EGIGSRKITYRLRDWLFSRQRYWGEPIPVIHWEDGETTLVPEDELPLLLPKATEIKPSGTGESPLANLHDWVNVTDKNGR
KGRRETNTMPQWAGSSWYFLRYIDPNNSEAIADKEKLAEWLPVDVYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQ
KLFNQGMILGENNEKMSKSRGNVVNPDEVVEKYGADTLRLYEMFMGPLEASIAWNENGLEGARKFLDRIWRLLVTEEGTL
AEKVTTDANANLEKAYHHMVKTVTNHYENLRFNTGISQLMIFINEAYKQDTIPKQYVEGFVQLLSPIAPHLAEELWEILG
HTETISYVAWPTYDETKLVEDEVEIVLQVNGKVKSKITVAKSLEKEELEKIAQEDNKMKENLEGKTIRKVIVVPGKLVNI
VAN
>Mature_802_residues
TFNHKKMEPKWQQYWSEHNTFKTTEDKNKDNFYALDMFPYPSGAGLHVGHPEGYTATDILSRMKRMQGKNVLHPIGWDAF
GLPAEQYAIDTGNDPEEFTALNIANFTRQIKSLGFSYDWDREINTTDPEYYKWTQWIFEKLYEKGLAYEAEIAVNWCPAL
GTVLANEEVIDGKSERGGFPVFRKPMRQWMLKITAYADRLLDDLDLVDWPENIKDMQRNWIGRSEGAEVTFKIKDSDETF
NVFTTRPDTLFGATYTVFAPEHELIEKITTPEQKEAVEAYKKQVELKSELERTDLAKDKTGVFTGAYAINPINGEEVPIW
IADYVLIQYGTGAIMAVPAHDERDFEFAQQFGLNIRPVLEGGDVTKEAFTGDGPHINSDFLNGLAKAEAITAAIDWLEKE
GIGSRKITYRLRDWLFSRQRYWGEPIPVIHWEDGETTLVPEDELPLLLPKATEIKPSGTGESPLANLHDWVNVTDKNGRK
GRRETNTMPQWAGSSWYFLRYIDPNNSEAIADKEKLAEWLPVDVYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQK
LFNQGMILGENNEKMSKSRGNVVNPDEVVEKYGADTLRLYEMFMGPLEASIAWNENGLEGARKFLDRIWRLLVTEEGTLA
EKVTTDANANLEKAYHHMVKTVTNHYENLRFNTGISQLMIFINEAYKQDTIPKQYVEGFVQLLSPIAPHLAEELWEILGH
TETISYVAWPTYDETKLVEDEVEIVLQVNGKVKSKITVAKSLEKEELEKIAQEDNKMKENLEGKTIRKVIVVPGKLVNIV
AN

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=875, Percent_Identity=33.4857142857143, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI94721239, Length=187, Percent_Identity=28.8770053475936, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI94721241, Length=187, Percent_Identity=28.8770053475936, Blast_Score=80, Evalue=8e-15,
Organism=Escherichia coli, GI1786861, Length=861, Percent_Identity=43.0894308943089, Blast_Score=697, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=408, Percent_Identity=25.4901960784314, Blast_Score=108, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71997510, Length=809, Percent_Identity=28.8009888751545, Blast_Score=323, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI71997517, Length=802, Percent_Identity=28.6783042394015, Blast_Score=322, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI212645227, Length=372, Percent_Identity=26.8817204301075, Blast_Score=135, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17510661, Length=454, Percent_Identity=24.8898678414097, Blast_Score=90, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17554638, Length=244, Percent_Identity=27.0491803278689, Blast_Score=73, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17541896, Length=214, Percent_Identity=27.1028037383178, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323414, Length=856, Percent_Identity=35.7476635514019, Blast_Score=480, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6319395, Length=189, Percent_Identity=29.1005291005291, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325097, Length=228, Percent_Identity=28.5087719298246, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21355409, Length=824, Percent_Identity=32.2815533980583, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI28574730, Length=699, Percent_Identity=22.8898426323319, Blast_Score=103, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24668547, Length=699, Percent_Identity=22.8898426323319, Blast_Score=103, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24668543, Length=699, Percent_Identity=22.8898426323319, Blast_Score=103, Evalue=6e-22,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 91940; Mature: 91809

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFNHKKMEPKWQQYWSEHNTFKTTEDKNKDNFYALDMFPYPSGAGLHVGHPEGYTATDI
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEECCCCCCCCHHHH
LSRMKRMQGKNVLHPIGWDAFGLPAEQYAIDTGNDPEEFTALNIANFTRQIKSLGFSYDW
HHHHHHHCCCCCCCCCCCCCCCCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
DREINTTDPEYYKWTQWIFEKLYEKGLAYEAEIAVNWCPALGTVLANEEVIDGKSERGGF
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHCCCHHCCCCCCCCCC
PVFRKPMRQWMLKITAYADRLLDDLDLVDWPENIKDMQRNWIGRSEGAEVTFKIKDSDET
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCE
FNVFTTRPDTLFGATYTVFAPEHELIEKITTPEQKEAVEAYKKQVELKSELERTDLAKDK
EEEEECCCCCCCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
TGVFTGAYAINPINGEEVPIWIADYVLIQYGTGAIMAVPAHDERDFEFAQQFGLNIRPVL
CCEEEEEEEECCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCHHHHHHHHCCCEEEEE
EGGDVTKEAFTGDGPHINSDFLNGLAKAEAITAAIDWLEKEGIGSRKITYRLRDWLFSRQ
CCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RYWGEPIPVIHWEDGETTLVPEDELPLLLPKATEIKPSGTGESPLANLHDWVNVTDKNGR
HHCCCCCCEEEECCCCEEECCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCC
KGRRETNTMPQWAGSSWYFLRYIDPNNSEAIADKEKLAEWLPVDVYIGGAEHAVLHLLYA
CCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHH
RFWHKFLYDIGVVPTKEPFQKLFNQGMILGENNEKMSKSRGNVVNPDEVVEKYGADTLRL
HHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCHHHHHCCCCCCCHHHHHHHHCCHHHHH
YEMFMGPLEASIAWNENGLEGARKFLDRIWRLLVTEEGTLAEKVTTDANANLEKAYHHMV
HHHHHCCHHCEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHH
KTVTNHYENLRFNTGISQLMIFINEAYKQDTIPKQYVEGFVQLLSPIAPHLAEELWEILG
HHHHHHHHCCEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
HTETISYVAWPTYDETKLVEDEVEIVLQVNGKVKSKITVAKSLEKEELEKIAQEDNKMKE
CCCEEEEEEECCCCCCHHHHCCEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
NLEGKTIRKVIVVPGKLVNIVAN
CCCCCCEEEEEECCCHHEEEECC
>Mature Secondary Structure 
TFNHKKMEPKWQQYWSEHNTFKTTEDKNKDNFYALDMFPYPSGAGLHVGHPEGYTATDI
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEECCCCCCCCHHHH
LSRMKRMQGKNVLHPIGWDAFGLPAEQYAIDTGNDPEEFTALNIANFTRQIKSLGFSYDW
HHHHHHHCCCCCCCCCCCCCCCCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
DREINTTDPEYYKWTQWIFEKLYEKGLAYEAEIAVNWCPALGTVLANEEVIDGKSERGGF
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHCCCHHCCCCCCCCCC
PVFRKPMRQWMLKITAYADRLLDDLDLVDWPENIKDMQRNWIGRSEGAEVTFKIKDSDET
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCE
FNVFTTRPDTLFGATYTVFAPEHELIEKITTPEQKEAVEAYKKQVELKSELERTDLAKDK
EEEEECCCCCCCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
TGVFTGAYAINPINGEEVPIWIADYVLIQYGTGAIMAVPAHDERDFEFAQQFGLNIRPVL
CCEEEEEEEECCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCHHHHHHHHCCCEEEEE
EGGDVTKEAFTGDGPHINSDFLNGLAKAEAITAAIDWLEKEGIGSRKITYRLRDWLFSRQ
CCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RYWGEPIPVIHWEDGETTLVPEDELPLLLPKATEIKPSGTGESPLANLHDWVNVTDKNGR
HHCCCCCCEEEECCCCEEECCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCC
KGRRETNTMPQWAGSSWYFLRYIDPNNSEAIADKEKLAEWLPVDVYIGGAEHAVLHLLYA
CCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHH
RFWHKFLYDIGVVPTKEPFQKLFNQGMILGENNEKMSKSRGNVVNPDEVVEKYGADTLRL
HHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCHHHHHCCCCCCCHHHHHHHHCCHHHHH
YEMFMGPLEASIAWNENGLEGARKFLDRIWRLLVTEEGTLAEKVTTDANANLEKAYHHMV
HHHHHCCHHCEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHH
KTVTNHYENLRFNTGISQLMIFINEAYKQDTIPKQYVEGFVQLLSPIAPHLAEELWEILG
HHHHHHHHCCEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
HTETISYVAWPTYDETKLVEDEVEIVLQVNGKVKSKITVAKSLEKEELEKIAQEDNKMKE
CCCEEEEEEECCCCCCHHHHCCEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
NLEGKTIRKVIVVPGKLVNIVAN
CCCCCCEEEEEECCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]