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The map label for this gene is trpB

Identifier: 226224229

GI number: 226224229

Start: 1654513

End: 1655715

Strand: Reverse

Name: trpB

Synonym: Lm4b_01639

Alternate gene names: 226224229

Gene position: 1655715-1654513 (Counterclockwise)

Preceding gene: 226224230

Following gene: 226224228

Centisome position: 56.84

GC content: 41.56

Gene sequence:

>1203_bases
ATGACTTATCAAGCACCTGACGAAAATGGCTTTTACGGAAAATTTGGCGGCAGATTTGTACCAGAAACATTAATGAAAGC
AGTGAAAGAATTAGACGAGGCGTACCGGGCTTCCAAAACAGATCCTGCTTTTCAAAAAGAATTAAACTATTATTTAAAAG
AATATGTGGGCCGAGAAACACCACTTTATTTTGCTGAACAATTAACAGCACATGCGGGCGGAGCAAAAATTTATTTAAAA
CGCGAAGATTTAAATCATACTGGCGCACATAAAATCAATAATACTATTGGGCAAGCCTTACTCGCACGTCAAATGGGTAA
ACAAAAAGTAGTGGCAGAAACTGGCGCAGGACAACACGGGGTGGCGACTGCAACTGTTGCAGCACTTTTTAATATGGAAT
GTACTATTTTTATGGGAGAAGAAGATGTGAAACGCCAATCACTCAATGTGTTTAGAATGGAACTTCTTGGTGCAAAAGTA
GTGAGCGTGAAAGCAGGAAGCAGAACATTAAAAGACGCAGTAAATGAGGCGCTCAGATTCTGGGTTGCTAATGTGGAAGA
CACGCATTACATTATGGGATCCGTTCTTGGACCGCATCCATTTCCAGAAATTGTTCGTGATTACCAAAGTGTGATAGGAA
TAGAAGCACGCAAACAACACTTGGAAAAAGAAGGTAAGCTTCCTGATGCAATTGTTGCTTGTGTTGGAGGCGGGAGTAAT
GCGATGGGGTTATTTTATCCATTTGTAGATGATGTTTCTGTTCAAATGCACGGTGTGGAAGCAGCGGGACACGGTTTAGA
AACTGAATTCCATGCAGCGACTATTTCCAAAGGAGAAATAGGGATTTTACACGGAGCGATGATGGACGTTTTACAAGATG
AGAATGGACAAATTTTGGAAGCATTTTCGATTTCAGCTGGCTTAGATTATCCAGGTATTGGTCCGGAGCATAGCTTTTTC
CGTGATTTAGGTCGTGCAGCGTACCATTCTGTTACCGATGATGAAGCCGTTGAAGCATTTCAACTTTTATGTCGTACGGA
AGGAATCATTCCTGCACTCGAAAGCTCGCACGCGATTAGTTATGCAGTCAAACTTGCAAGCCAAATGCGTCCAGAAGAAA
GTATGGTCGTTTGTTTATCTGGTCGCGGAGATAAAGATGTTAATCAACTAAAGGAACGCTTGGAGGGACAAATAAATGAC
TAA

Upstream 100 bases:

>100_bases
CATATGCAGTAGATATTTCCTCTGGCGTAGAAACAAACGGAGAAAAAGATCCGGAGAAAATTAAATGCTTCATTAAAACA
GCAAAAGGAGTGGAATAATA

Downstream 100 bases:

>100_bases
AACATTAACGAATAAACTAGCGAAAAAAGATCATGCTGCCGTAGTTACTTACATTATGGGTGGCGATGGTGGCTTAGATA
ATTTAGAAGAACAGTTATTG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTYQAPDENGFYGKFGGRFVPETLMKAVKELDEAYRASKTDPAFQKELNYYLKEYVGRETPLYFAEQLTAHAGGAKIYLK
REDLNHTGAHKINNTIGQALLARQMGKQKVVAETGAGQHGVATATVAALFNMECTIFMGEEDVKRQSLNVFRMELLGAKV
VSVKAGSRTLKDAVNEALRFWVANVEDTHYIMGSVLGPHPFPEIVRDYQSVIGIEARKQHLEKEGKLPDAIVACVGGGSN
AMGLFYPFVDDVSVQMHGVEAAGHGLETEFHAATISKGEIGILHGAMMDVLQDENGQILEAFSISAGLDYPGIGPEHSFF
RDLGRAAYHSVTDDEAVEAFQLLCRTEGIIPALESSHAISYAVKLASQMRPEESMVVCLSGRGDKDVNQLKERLEGQIND

Sequences:

>Translated_400_residues
MTYQAPDENGFYGKFGGRFVPETLMKAVKELDEAYRASKTDPAFQKELNYYLKEYVGRETPLYFAEQLTAHAGGAKIYLK
REDLNHTGAHKINNTIGQALLARQMGKQKVVAETGAGQHGVATATVAALFNMECTIFMGEEDVKRQSLNVFRMELLGAKV
VSVKAGSRTLKDAVNEALRFWVANVEDTHYIMGSVLGPHPFPEIVRDYQSVIGIEARKQHLEKEGKLPDAIVACVGGGSN
AMGLFYPFVDDVSVQMHGVEAAGHGLETEFHAATISKGEIGILHGAMMDVLQDENGQILEAFSISAGLDYPGIGPEHSFF
RDLGRAAYHSVTDDEAVEAFQLLCRTEGIIPALESSHAISYAVKLASQMRPEESMVVCLSGRGDKDVNQLKERLEGQIND
>Mature_399_residues
TYQAPDENGFYGKFGGRFVPETLMKAVKELDEAYRASKTDPAFQKELNYYLKEYVGRETPLYFAEQLTAHAGGAKIYLKR
EDLNHTGAHKINNTIGQALLARQMGKQKVVAETGAGQHGVATATVAALFNMECTIFMGEEDVKRQSLNVFRMELLGAKVV
SVKAGSRTLKDAVNEALRFWVANVEDTHYIMGSVLGPHPFPEIVRDYQSVIGIEARKQHLEKEGKLPDAIVACVGGGSNA
MGLFYPFVDDVSVQMHGVEAAGHGLETEFHAATISKGEIGILHGAMMDVLQDENGQILEAFSISAGLDYPGIGPEHSFFR
DLGRAAYHSVTDDEAVEAFQLLCRTEGIIPALESSHAISYAVKLASQMRPEESMVVCLSGRGDKDVNQLKERLEGQIND

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=390, Percent_Identity=52.5641025641026, Blast_Score=435, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6321412, Length=384, Percent_Identity=54.1666666666667, Blast_Score=454, Evalue=1e-128,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 43790; Mature: 43659

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYQAPDENGFYGKFGGRFVPETLMKAVKELDEAYRASKTDPAFQKELNYYLKEYVGRET
CCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
PLYFAEQLTAHAGGAKIYLKREDLNHTGAHKINNTIGQALLARQMGKQKVVAETGAGQHG
CHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCC
VATATVAALFNMECTIFMGEEDVKRQSLNVFRMELLGAKVVSVKAGSRTLKDAVNEALRF
HHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHH
WVANVEDTHYIMGSVLGPHPFPEIVRDYQSVIGIEARKQHLEKEGKLPDAIVACVGGGSN
HHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCC
AMGLFYPFVDDVSVQMHGVEAAGHGLETEFHAATISKGEIGILHGAMMDVLQDENGQILE
CCEEEECCCCCCEEEEECCHHCCCCCCCCHHHEEECCCCCHHHHHHHHHHHHCCCCCEEE
AFSISAGLDYPGIGPEHSFFRDLGRAAYHSVTDDEAVEAFQLLCRTEGIIPALESSHAIS
EEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHH
YAVKLASQMRPEESMVVCLSGRGDKDVNQLKERLEGQIND
HHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TYQAPDENGFYGKFGGRFVPETLMKAVKELDEAYRASKTDPAFQKELNYYLKEYVGRET
CCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
PLYFAEQLTAHAGGAKIYLKREDLNHTGAHKINNTIGQALLARQMGKQKVVAETGAGQHG
CHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCC
VATATVAALFNMECTIFMGEEDVKRQSLNVFRMELLGAKVVSVKAGSRTLKDAVNEALRF
HHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHH
WVANVEDTHYIMGSVLGPHPFPEIVRDYQSVIGIEARKQHLEKEGKLPDAIVACVGGGSN
HHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCC
AMGLFYPFVDDVSVQMHGVEAAGHGLETEFHAATISKGEIGILHGAMMDVLQDENGQILE
CCEEEECCCCCCEEEEECCHHCCCCCCCCHHHEEECCCCCHHHHHHHHHHHHCCCCCEEE
AFSISAGLDYPGIGPEHSFFRDLGRAAYHSVTDDEAVEAFQLLCRTEGIIPALESSHAIS
EEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHH
YAVKLASQMRPEESMVVCLSGRGDKDVNQLKERLEGQIND
HHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]