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The map label for this gene is trpF

Identifier: 226224230

GI number: 226224230

Start: 1655718

End: 1656326

Strand: Reverse

Name: trpF

Synonym: Lm4b_01640

Alternate gene names: 226224230

Gene position: 1656326-1655718 (Counterclockwise)

Preceding gene: 226224231

Following gene: 226224229

Centisome position: 56.87

GC content: 38.42

Gene sequence:

>609_bases
ATGATTGTAAAAATTTGTGGATTGAAAAAAGCAGTAGATGTAGCGGCTGCGGTCGATAATGGCGCAGATATGATTGGCTT
CGTTTTCGCAAAAAGTAAACGCCAAGTTACGGTGGAAAAGGCACATGAATTAGCTAAAAATATTCCCGCTAACGTCAAAA
AAGTCGGGGTATTCGTTAATCCCACTGAAGAAGAGTTAAAGGCAGCAATCAAAGGTGTGCCATTAGACATTGTTCAACTT
CACGGACAAGAACCCGCAAAACAAGCAAATCGCACAGATGCAGAAGTAATCAAAGCTTTTCCTGTGAAAGATGGGAAACT
TCCTACCAATATAAATGACTATCCAAACGCTTATATTTTACTTGATGCGCCAGCAGAGGAATATGAAGGCGGCAGCGGAA
AAACATTTGATTGGGATAAAATAAATAGAGACATGCTTACAAAAAACAAATTAATTATAGCTGGCGGATTAAATGCTCAG
AATGTACAAGAAGCCATTAAACGCTTTGAACCATATGCAGTAGATATTTCCTCTGGCGTAGAAACAAACGGAGAAAAAGA
TCCGGAGAAAATTAAATGCTTCATTAAAACAGCAAAAGGAGTGGAATAA

Upstream 100 bases:

>100_bases
TGGCTCGGGTTAGTCAAAAATATAATGCAGTACTTGTTGGAGAAGCGCTAATGCGAGAAGCGACTCCAGAAGCAGCGGCG
AAAAGTTTGAAGGTGACACG

Downstream 100 bases:

>100_bases
TAATGACTTATCAAGCACCTGACGAAAATGGCTTTTACGGAAAATTTGGCGGCAGATTTGTACCAGAAACATTAATGAAA
GCAGTGAAAGAATTAGACGA

Product: N-(5'-phosphoribosyl)anthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MIVKICGLKKAVDVAAAVDNGADMIGFVFAKSKRQVTVEKAHELAKNIPANVKKVGVFVNPTEEELKAAIKGVPLDIVQL
HGQEPAKQANRTDAEVIKAFPVKDGKLPTNINDYPNAYILLDAPAEEYEGGSGKTFDWDKINRDMLTKNKLIIAGGLNAQ
NVQEAIKRFEPYAVDISSGVETNGEKDPEKIKCFIKTAKGVE

Sequences:

>Translated_202_residues
MIVKICGLKKAVDVAAAVDNGADMIGFVFAKSKRQVTVEKAHELAKNIPANVKKVGVFVNPTEEELKAAIKGVPLDIVQL
HGQEPAKQANRTDAEVIKAFPVKDGKLPTNINDYPNAYILLDAPAEEYEGGSGKTFDWDKINRDMLTKNKLIIAGGLNAQ
NVQEAIKRFEPYAVDISSGVETNGEKDPEKIKCFIKTAKGVE
>Mature_202_residues
MIVKICGLKKAVDVAAAVDNGADMIGFVFAKSKRQVTVEKAHELAKNIPANVKKVGVFVNPTEEELKAAIKGVPLDIVQL
HGQEPAKQANRTDAEVIKAFPVKDGKLPTNINDYPNAYILLDAPAEEYEGGSGKTFDWDKINRDMLTKNKLIIAGGLNAQ
NVQEAIKRFEPYAVDISSGVETNGEKDPEKIKCFIKTAKGVE

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=205, Percent_Identity=32.6829268292683, Blast_Score=79, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320210, Length=218, Percent_Identity=32.1100917431193, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_LISMC (C1KVS6)

Other databases:

- EMBL:   FM242711
- RefSeq:   YP_002758337.1
- GeneID:   7701609
- GenomeReviews:   FM242711_GR
- KEGG:   lmc:Lm4b_01640
- OMA:   SKICGIT
- ProtClustDB:   PRK01222
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 21962; Mature: 21962

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVKICGLKKAVDVAAAVDNGADMIGFVFAKSKRQVTVEKAHELAKNIPANVKKVGVFVN
CEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCEEHHHHHHHHHHCCCCCCEEEEEEEC
PTEEELKAAIKGVPLDIVQLHGQEPAKQANRTDAEVIKAFPVKDGKLPTNINDYPNAYIL
CCHHHHHHHHCCCCEEEEEECCCCCHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEE
LDAPAEEYEGGSGKTFDWDKINRDMLTKNKLIIAGGLNAQNVQEAIKRFEPYAVDISSGV
EECCHHHCCCCCCCCCCHHHCCHHHHCCCCEEEEECCCHHHHHHHHHHCCCEEEEECCCC
ETNGEKDPEKIKCFIKTAKGVE
CCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIVKICGLKKAVDVAAAVDNGADMIGFVFAKSKRQVTVEKAHELAKNIPANVKKVGVFVN
CEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCEEHHHHHHHHHHCCCCCCEEEEEEEC
PTEEELKAAIKGVPLDIVQLHGQEPAKQANRTDAEVIKAFPVKDGKLPTNINDYPNAYIL
CCHHHHHHHHCCCCEEEEEECCCCCHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEE
LDAPAEEYEGGSGKTFDWDKINRDMLTKNKLIIAGGLNAQNVQEAIKRFEPYAVDISSGV
EECCHHHCCCCCCCCCCHHHCCHHHHCCCCEEEEECCCHHHHHHHHHHCCCEEEEECCCC
ETNGEKDPEKIKCFIKTAKGVE
CCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA