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The map label for this gene is trpA

Identifier: 226224228

GI number: 226224228

Start: 1653747

End: 1654520

Strand: Reverse

Name: trpA

Synonym: Lm4b_01638

Alternate gene names: 226224228

Gene position: 1654520-1653747 (Counterclockwise)

Preceding gene: 226224229

Following gene: 226224227

Centisome position: 56.8

GC content: 38.89

Gene sequence:

>774_bases
ATGACTAAAACATTAACGAATAAACTAGCGAAAAAAGATCATGCTGCCGTAGTTACTTACATTATGGGTGGCGATGGTGG
CTTAGATAATTTAGAAGAACAGTTATTGTTTCTCGAAAAATCTGGTGTAAGCGCGATTGAAATTGGTATTCCTTTTTCTG
ATCCGGTTGCCGACGGTCCGATTATTCAACTTGCTGGGTTACGTGCTTTAAAGGAGCAAGTGAGTTTAGAAGCCATTTTA
AATAAATTGGCTAGGAGTAAAGTGCAAATTCCGCTAATTATTATGAGTTATATTAATCCGATTTTTCATTTAGGTATCCC
GAAATTTGTTGAATTGGTGCAGAAAACGCCGGTGAAAGGGCTTATTATTCCTGATTTACCTTATGAGCACCAAACACTGA
TTACGCCAGAACTTCAAGGGACAGATATCGCACTTATTCCGCTTGTTTCATTGACAAGTCCGAAAGAGCGCCTCGAAGAA
ATTGCGAAACAGGCAGAAGGGTTTATTTATGCAGTAACAGTTAACGGAACTACCGGTGTGAGGAGTGAATTTGATGCCCA
TATTGATAACCATTTAGCTTATTTAAAAAGCATTAGCCCTGTTCCAGTTCTCGCAGGTTTTGGTGTTTCTTCTATTGAAC
ATGTGGAAAAATTTGCGCATGTATGTGATGGTGTAATAATCGGTAGTAAAGTCGTGCAAATGTTACATGAAGAAAAAACG
GCAGAGCTAGGAGCGTTTTTACAAAAGGCCGCAGAAGTTCGAATCGAAAACTAA

Upstream 100 bases:

>100_bases
GCAAGCCAAATGCGTCCAGAAGAAAGTATGGTCGTTTGTTTATCTGGTCGCGGAGATAAAGATGTTAATCAACTAAAGGA
ACGCTTGGAGGGACAAATAA

Downstream 100 bases:

>100_bases
TTACTACTTACAATTTCTCTTTAAATATGGTAAAGTCTGGTGTATAGAATGTTAAAGAGGAGTTGTAAGGATGTCTGTTG
AAGTTAAACCAGAGCATGCG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTKTLTNKLAKKDHAAVVTYIMGGDGGLDNLEEQLLFLEKSGVSAIEIGIPFSDPVADGPIIQLAGLRALKEQVSLEAIL
NKLARSKVQIPLIIMSYINPIFHLGIPKFVELVQKTPVKGLIIPDLPYEHQTLITPELQGTDIALIPLVSLTSPKERLEE
IAKQAEGFIYAVTVNGTTGVRSEFDAHIDNHLAYLKSISPVPVLAGFGVSSIEHVEKFAHVCDGVIIGSKVVQMLHEEKT
AELGAFLQKAAEVRIEN

Sequences:

>Translated_257_residues
MTKTLTNKLAKKDHAAVVTYIMGGDGGLDNLEEQLLFLEKSGVSAIEIGIPFSDPVADGPIIQLAGLRALKEQVSLEAIL
NKLARSKVQIPLIIMSYINPIFHLGIPKFVELVQKTPVKGLIIPDLPYEHQTLITPELQGTDIALIPLVSLTSPKERLEE
IAKQAEGFIYAVTVNGTTGVRSEFDAHIDNHLAYLKSISPVPVLAGFGVSSIEHVEKFAHVCDGVIIGSKVVQMLHEEKT
AELGAFLQKAAEVRIEN
>Mature_256_residues
TKTLTNKLAKKDHAAVVTYIMGGDGGLDNLEEQLLFLEKSGVSAIEIGIPFSDPVADGPIIQLAGLRALKEQVSLEAILN
KLARSKVQIPLIIMSYINPIFHLGIPKFVELVQKTPVKGLIIPDLPYEHQTLITPELQGTDIALIPLVSLTSPKERLEEI
AKQAEGFIYAVTVNGTTGVRSEFDAHIDNHLAYLKSISPVPVLAGFGVSSIEHVEKFAHVCDGVIIGSKVVQMLHEEKTA
ELGAFLQKAAEVRIEN

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=217, Percent_Identity=30.4147465437788, Blast_Score=102, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321412, Length=239, Percent_Identity=35.5648535564854, Blast_Score=152, Evalue=5e-38,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 27902; Mature: 27771

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTLTNKLAKKDHAAVVTYIMGGDGGLDNLEEQLLFLEKSGVSAIEIGIPFSDPVADGP
CCCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
IIQLAGLRALKEQVSLEAILNKLARSKVQIPLIIMSYINPIFHLGIPKFVELVQKTPVKG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCE
LIIPDLPYEHQTLITPELQGTDIALIPLVSLTSPKERLEEIAKQAEGFIYAVTVNGTTGV
EEECCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCEEEEEEECCCCCC
RSEFDAHIDNHLAYLKSISPVPVLAGFGVSSIEHVEKFAHVCDGVIIGSKVVQMLHEEKT
HHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AELGAFLQKAAEVRIEN
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKTLTNKLAKKDHAAVVTYIMGGDGGLDNLEEQLLFLEKSGVSAIEIGIPFSDPVADGP
CCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
IIQLAGLRALKEQVSLEAILNKLARSKVQIPLIIMSYINPIFHLGIPKFVELVQKTPVKG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCE
LIIPDLPYEHQTLITPELQGTDIALIPLVSLTSPKERLEEIAKQAEGFIYAVTVNGTTGV
EEECCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCEEEEEEECCCCCC
RSEFDAHIDNHLAYLKSISPVPVLAGFGVSSIEHVEKFAHVCDGVIIGSKVVQMLHEEKT
HHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AELGAFLQKAAEVRIEN
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]